C. N. Lakshminarayana Reddy, M. Mantesh, A. Chowdappa, H. Shridhar, V. Venkataravanappa
{"title":"Candidatus Phytoplasma aurantifolia’ (16SrII-C) associated with phyllody disease of snake gourd from India","authors":"C. N. Lakshminarayana Reddy, M. Mantesh, A. Chowdappa, H. Shridhar, V. Venkataravanappa","doi":"10.1007/s13314-024-00526-5","DOIUrl":"10.1007/s13314-024-00526-5","url":null,"abstract":"<div><p>Typical symptoms of little leaf such as yellowing, phyllody, virescence, and witches' broom were observed on snake gourd in the Jalna district of Maharashtra state, India. Total genomic DNA extracted from symptomatic plants was subjected to PCR assay using the 16S rRNA gene specific primer pair. Pairwise comparison of the 16S rRNA gene sequence (1.2 kb) of the Snake gourd phytoplasma isolate showed it shared 100% sequence identity with Crotalaria phyllody-[16SrII-C] phytoplasma (<i>Candidatus</i> Phytoplasma aurantifolia). Further virtual RFLP analysis of 16Sr RNA gene sequence of F2 region of the snake gourd phytoplasma isolate showed a similarity coefficient of 0.99 with the 16SrII-C subgroup. Here we have described <i>Candidatus</i> Phytoplasma aurantifolia (16SrII-C) infecting snake gourd in India.</p></div>","PeriodicalId":45862,"journal":{"name":"Australasian Plant Disease Notes","volume":"19 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139807586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Karyn Engelbrecht, Inge Raubenheimer, Trudy Paap, Emma Neethling, Francois Roets
{"title":"Detection of Fusarium euwallaceae and its vector Euwallacea fornicatus on pear (Pyrus communis) and in deciduous fruit orchards in South Africa","authors":"Karyn Engelbrecht, Inge Raubenheimer, Trudy Paap, Emma Neethling, Francois Roets","doi":"10.1007/s13314-023-00524-z","DOIUrl":"10.1007/s13314-023-00524-z","url":null,"abstract":"<div><p>We present the first report of <i>Fusarium euwallaceae</i> and its ambrosia beetle vector <i>Euwallacea fornicatus</i> in deciduous fruit tree orchards in South Africa. <i>Fusarium euwallaceae</i>, is shown to be pathogenic to pear (<i>Pyrus communis</i>) and the beetle can establish viable colonies in this host.</p></div>","PeriodicalId":45862,"journal":{"name":"Australasian Plant Disease Notes","volume":"19 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13314-023-00524-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139614423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"First report of Cercospora Rumicis in Australia","authors":"Sophia Eleanor Callaghan, Lucy Horne","doi":"10.1007/s13314-023-00522-1","DOIUrl":"10.1007/s13314-023-00522-1","url":null,"abstract":"<div><p>A leaf spot disease was observed on red veined sorrel (<i>Rumex sanguineus</i>) growing in a commercial greenhouse in northern NSW, Australia, in July 2021. <i>Cercospora rumicis</i> was found in association with the leaf spot and was identified morphologically and by sequencing two gene regions, histone-3 (HIS) and calmodulin (CAL). Koch’s postulates were fulfilled through pathogenicity tests, including the inoculation of potted plants and a detached leaf assay. This is the first report of <i>Cercospora rumicis</i> in Australia.</p></div>","PeriodicalId":45862,"journal":{"name":"Australasian Plant Disease Notes","volume":"18 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138473351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aleksandra S. Orina, Tatiana Yu Gagkaeva, Olga P. Gavrilova
{"title":"Novel cruciferous plant host of Fusarium temperatum, a species mainly associated with maize","authors":"Aleksandra S. Orina, Tatiana Yu Gagkaeva, Olga P. Gavrilova","doi":"10.1007/s13314-023-00523-0","DOIUrl":"10.1007/s13314-023-00523-0","url":null,"abstract":"<div><p>Using phylogenetic analysis of three loci of <i>Fusarium subglutinans</i> sensu lato strains isolated from wheat and rape, the strains were re-identified as <i>F. temperatum</i>. This is the first report of this fungus in a cruciferous plant’s mycobiota<i>.</i> Analysis of <i>F. temperatum</i> pathogenicity to rape revealed that the pathogen reduced the germination of seeds by 15%, and decreased seedling length by 13–18%. <i>Fusarium temperatum</i> strains did not produce fumonisins, but produced beauvericin and moniliformin.</p></div>","PeriodicalId":45862,"journal":{"name":"Australasian Plant Disease Notes","volume":"18 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138473290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Geographic range extension of Ustilago sporoboli-indici on Sporobolus natalensis in Australia","authors":"D.I. Officer, R.G. Shivas, J.S. Vitelli","doi":"10.1007/s13314-023-00521-2","DOIUrl":"10.1007/s13314-023-00521-2","url":null,"abstract":"<div><p>Field surveys during 2021–2023 found that the leaf smut fungus <i>Ustilago sporoboli-indici</i> was widespread on <i>Sporobolus natalensis</i> from northern New South Wales to northern Queensland. The geographic range of <i>Ustilago sporoboli-indici</i> has extended to 1500 km following the first Australian record of <i>U. sporoboli-indici</i> in south-east Queensland in 2017. <i>Ustilago sporoboli-indici</i> was not observed on other cohabitating species of <i>Sporobolus.</i></p></div>","PeriodicalId":45862,"journal":{"name":"Australasian Plant Disease Notes","volume":"18 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134796459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pablo Llumiquinga, Wladimir J. Enríquez, Karina Proaño, Patricio Gallegos, Carlos Gutiérrez-Gutiérrez
{"title":"Detection and molecular characterization of the northern root-knot nematode, Meloidogyne hapla, infesting a tree tomato field in Ecuador","authors":"Pablo Llumiquinga, Wladimir J. Enríquez, Karina Proaño, Patricio Gallegos, Carlos Gutiérrez-Gutiérrez","doi":"10.1007/s13314-023-00520-3","DOIUrl":"10.1007/s13314-023-00520-3","url":null,"abstract":"<div><p><i>Meloidogyne hapla</i> was detected for the sixth time in Ecuador; however this species was recorded for the first time infesting a tree tomato (<i>Solanum betaceum</i> Cav.) crop in this country. It represents the first record for this species in the Tungurahua province, Ecuador. These results confirm a wider geographical distribution pattern of this species in Ecuador and a wide range of host species. The identification was based on the morphology of female perineal pattern and molecular data. Contrasting morphological hypotheses with molecular data provided rapid and accurate identification of <i>M</i>. <i>hapla</i>.</p></div>","PeriodicalId":45862,"journal":{"name":"Australasian Plant Disease Notes","volume":"18 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134795503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Viengvilay Vorlachith, Somchith Khanthilad, Phitsamay Phitsanoukane, Khamphou Sengdala, Khonesavane Chittarhat, Jillian Lyall, Len Tesoriero, Jordan Bailey, Wendy Forbes, Sophia Callaghan, Adam Williams, Lester Burgess
{"title":"First report of Plasmodiophora brassicae in Lao People’s Democratic Republic","authors":"Viengvilay Vorlachith, Somchith Khanthilad, Phitsamay Phitsanoukane, Khamphou Sengdala, Khonesavane Chittarhat, Jillian Lyall, Len Tesoriero, Jordan Bailey, Wendy Forbes, Sophia Callaghan, Adam Williams, Lester Burgess","doi":"10.1007/s13314-023-00519-w","DOIUrl":"10.1007/s13314-023-00519-w","url":null,"abstract":"<div><p>Cabbage production is promoted as a cash crop for small-land holders to support poverty alleviation on the Bolaven Plateau in Champasak Province, Lao People’s Democratic Republic (PDR). A stunted cabbage crop in Paksong was inspected due to a near total yield loss. Examination of the root systems revealed the presence of enlarged roots. Subsequently the disease was found in another round-head cabbage crop, and in a Chinese cabbage crop in a polyhouse. Air-dried clubroot specimens were forwarded to Australia where molecular methods confirmed the presence of <i>Plasmodiophora brassicae.</i> This is the first report of the obligate pathogen <i>Plasmodiophora brassicae</i> in the Lao PDR.</p></div>","PeriodicalId":45862,"journal":{"name":"Australasian Plant Disease Notes","volume":"18 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13314-023-00519-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50040007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Matthew H. Laurence, Allison A. Mertin, Clara Pang, Ossie Wildman, Andrew M. Daly, Brett A. Summerell
{"title":"First report of Phytophthora plurivora in Australia","authors":"Matthew H. Laurence, Allison A. Mertin, Clara Pang, Ossie Wildman, Andrew M. Daly, Brett A. Summerell","doi":"10.1007/s13314-023-00518-x","DOIUrl":"10.1007/s13314-023-00518-x","url":null,"abstract":"<div><p>As part of the International Plant Sentinel Network program monitoring high priority plant species from the United Kingdom, soil samples were collected from an asymptomatic <i>Quercus robur</i> (English Oak) at the Blue Mountains Botanic Garden, Mount Tomah (NSW). The soil samples were baited for <i>Phytophthora</i> with detection based on a <i>Phytophthora</i> genus specific Taqman™ assay. Species were identified using Sanger sequencing and phylogenetic analysis of the mitochondrial DNA subunit cytochrome c oxidase subunit 1 (<i>COI</i>) and the nuclear ribosomal DNA internal transcribed spacer 1, 5.8S, ITS2 and partial 28S regions (ITS). Isolates of <i>Phytophthora</i> that were recovered were identical to the <i>Phytophthora plurivora</i> ex-type strain based on phylogenetic inference. This is the first identification of <i>Phytophthora plurivora</i> in Australia.</p></div>","PeriodicalId":45862,"journal":{"name":"Australasian Plant Disease Notes","volume":"18 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50021577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Molecular characterization of a begomovirus associated with leaf crumpling and severe mosaic disease of bell pepper","authors":"Priya Lager, Yogesh Kumar","doi":"10.1007/s13314-023-00517-y","DOIUrl":"10.1007/s13314-023-00517-y","url":null,"abstract":"<div><p>Bell pepper plant leaves showing typical begomovirus-like symptoms (crumpling, mosaic, and stunting) were collected from Jalandhar region of Punjab, India. Total DNA was isolated from symptomatic plant leaves, and species-specific primers were used to amplify and detect begomovirus. The full-length genome was amplified using the rolling circle amplification method, cloned into the pBluescript II KS (+) vector, and sequenced. A BLAST search of the current DNA-A isolate revealed the highest percentage of identity (98.83%) with other isolates. Betasatellite sequences on BLAST search showed a close similarity of 98.10% with other isolates. Phylogenetic analysis with other distinct begomovirus species revealed that the virus’s current DNA-A was closely related to the <i>Papaya leaf crumple virus</i> and the <i>Chenopodium leaf distortion virus</i>. For the present isolates of DNA-A and betasatellite, no major recombination event was found. In the present study, molecular identification and characterization of the <i>Papaya leaf crumple virus</i> with <i>Papaya leaf curl betasatellite</i> adds another host, and to the best of our knowledge, this is the first report of the present virus infecting the bell pepper plant worldwide.</p></div>","PeriodicalId":45862,"journal":{"name":"Australasian Plant Disease Notes","volume":"18 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50015059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Giorgio Gusella, Chiara Di Pietro, Laura Vecchio, Giuseppe Campo, Giancarlo Polizzi
{"title":"Branch canker and dieback of Meryta denhamii caused by Neofusicoccum parvum and Neoscytalidium dimidiatum in Italy","authors":"Giorgio Gusella, Chiara Di Pietro, Laura Vecchio, Giuseppe Campo, Giancarlo Polizzi","doi":"10.1007/s13314-023-00515-0","DOIUrl":"10.1007/s13314-023-00515-0","url":null,"abstract":"<div><p>A recent survey conducted in the historical botanical garden Villa Caronia, Sicily, identified the causal organisms of branch cankers and dieback on a mature <i>Meryta denhamii</i>. Laboratory isolations and sequencing of three loci, namely ITS, <i>tef1-α</i>, and <i>tub2</i>, showed the presence of two species belonging to Botryosphaeriaceae, including <i>Neofusicoccum parvum</i> and <i>Neoscytalidium dimidiatum.</i> Pathogenicity tests confirmed that these species are able to induce canker symptoms on this host. To our knowledge, this is the first report, worldwide, of <i>N. parvum</i> and <i>Ne. dimidiatum</i> causing branch canker and dieback on <i>M. denhamii</i>.</p></div>","PeriodicalId":45862,"journal":{"name":"Australasian Plant Disease Notes","volume":"18 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41516567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}