Candidatus Phytoplasma aurantifolia’ (16SrII-C) associated with phyllody disease of snake gourd from India

IF 0.6 Q4 PLANT SCIENCES
C. N. Lakshminarayana Reddy, M. Mantesh, A. Chowdappa, H. Shridhar, V. Venkataravanappa
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引用次数: 0

Abstract

Typical symptoms of little leaf such as yellowing, phyllody, virescence, and witches' broom were observed on snake gourd in the Jalna district of Maharashtra state, India. Total genomic DNA extracted from symptomatic plants was subjected to PCR assay using the 16S rRNA gene specific primer pair. Pairwise comparison of the 16S rRNA gene sequence (1.2 kb) of the Snake gourd phytoplasma isolate showed it shared 100% sequence identity with Crotalaria phyllody-[16SrII-C] phytoplasma (Candidatus Phytoplasma aurantifolia). Further virtual RFLP analysis of 16Sr RNA gene sequence of F2 region of the snake gourd phytoplasma isolate showed a similarity coefficient of 0.99 with the 16SrII-C subgroup. Here we have described Candidatus Phytoplasma aurantifolia (16SrII-C) infecting snake gourd in India.

与印度蛇葫植物病害有关的 "枳壳植原体"(16SrII-C)菌株
在印度马哈拉施特拉邦贾尔纳地区的蛇瓜上观察到了典型的小叶病症状,如黄化、叶斑病、病毒病和女巫扫帚病。使用 16S rRNA 基因特异引物对从有症状植物中提取的总基因组 DNA 进行 PCR 检测。蛇葫植物支原体分离物的 16S rRNA 基因序列(1.2 kb)配对比较显示,它与 Crotalaria phyllody-[16SrII-C] 植物支原体(Candidatus Phytoplasma aurantifolia)具有 100%的序列同一性。对蛇瓜植原体 F2 区 16Sr RNA 基因序列的进一步虚拟 RFLP 分析表明,其与 16SrII-C 亚群的相似系数为 0.99。在此,我们描述了感染印度蛇葫芦的枳壳蝇蛆病原菌(16SrII-C)。
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来源期刊
CiteScore
1.90
自引率
0.00%
发文量
36
期刊介绍: Australasian Plant Disease Notes is an international journal for publication of short reports from all countries which describe original research that transcends national boundaries. All aspects of plant pathology are covered including new geographical records of diseases or pathogens, new pathogens or pathogen strains, taxonomic papers, quarantine notes, disease management and diagnostic methods. Australasian Plant Disease Notes is a publication of the Australasian Plant Pathology Society.
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