Madeline Robles, Rachael M Carew, Carolyn Rando, Sherry Nakhaeizadeh, Ruth M Morgan
{"title":"Sex estimation from virtual models: exploring the potential of stereolithic 3D crania models for morphoscopic trait scoring.","authors":"Madeline Robles, Rachael M Carew, Carolyn Rando, Sherry Nakhaeizadeh, Ruth M Morgan","doi":"10.1093/fsr/owad017","DOIUrl":"https://doi.org/10.1093/fsr/owad017","url":null,"abstract":"<p><p>Modern computed tomography (CT) databases are becoming an accepted resource for the practice and development of identification methods in forensic anthropology. However, the utility of 3D models created using free and open-source visualization software such as 3D Slicer has not yet been thoroughly assessed for morphoscopic biological profiling methods where virtual methods of analysis are becoming more common. This paper presents a study that builds on the initial findings from Robles et al. (2020) to determine the feasibility of estimating sex on stereolithic (STL) 3D cranial models produced from CT scans from a modern, living UK population (<i>n</i> = 80) using equation 2 from the Walker's (2008) morphoscopic method. Kendall's coefficients of concordance (KCC) indicated substantial agreement using cranial features scores in an inter-observer test and a video-inter-observer test. Fleiss' Kappa scores showed moderate agreement (0.50) overall between inter-observer sex estimations, and for observer sex estimations in comparison to recorded sexes (0.56). It was found that novice users could virtually employ morphoscopic sex estimation methods effectively on STL 3D cranial models from modern individuals. This study also highlights the potential that digital databases of modern living populations can offer forensic anthropology.</p><p><strong>Key points: </strong>First example of Walker's (2008) method applied to a living UK population.Open-source software is a valuable resource for crime reconstruction approaches.Male scoring bias was observed in method application.Forensic anthropologists would benefit from virtual anthropology training to use and interpret 3D models.Digital databases offer more ethical, diverse, modern populations for future research.</p>","PeriodicalId":45852,"journal":{"name":"Forensic Sciences Research","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10445579/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10260841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The potential of using non-coding RNAs in forensic science applications.","authors":"Yawen Li, Zhuoqun Wang, Dikeledi Ishmael, Yehui Lvy","doi":"10.1093/fsr/owad003","DOIUrl":"https://doi.org/10.1093/fsr/owad003","url":null,"abstract":"<p><p>With the continuous development and integration of molecular biology and forensic science, non-coding RNAs (ncRNAs), especially ncRNAs with regulatory functions such as microRNA, long non-coding RNA, and circular RNA, have recently been actively explored by forensic scholars. In this study, we review the literature on these ncRNAs in various fields of forensic science, including postmortem interval determination, wound age estimation, forensic age assessment, cause of death analysis, and body fluid identification, aiming to evaluate the current research and provide a perspective for future applications.</p>","PeriodicalId":45852,"journal":{"name":"Forensic Sciences Research","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10445561/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10262456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Incidental Findings in Forensic Investigations: A Narrative Review","authors":"Giulia Fasani, S. Gino, Giulia Sguazzi","doi":"10.3390/forensicsci3020026","DOIUrl":"https://doi.org/10.3390/forensicsci3020026","url":null,"abstract":"Incidental findings (IFs) are unexpected disclosures that do not fall within the aim of a test and have a potential impact on an individual’s life. In the forensic field, IFs can be considered information that is not related to the cause of death, the dynamic of the event, or the scope of the investigation. Questions regarding how forensic professionals should consider, address, and report IFs form the focus of our study. This narrative review was performing following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines with the aim of defining and summarizing evidence about IFs, analyzing their ethical and legal management, and emphasizing the importance of adequate informed consent. The current state of the art regarding IFs has revealed the need for guidelines with the purpose of making the best decisions for the parties involved since the circumstances are extremely delicate, and therefore, forensic geneticists require protection and support. For this reason, elaboration of informed consent, which considers the possibility of making accidental discoveries, could operate as an immediate solution to fill the current gaps. However, the development of clear international recommendations is the best solution to such a complex issue.","PeriodicalId":45852,"journal":{"name":"Forensic Sciences Research","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76873376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Zughaibi, Latifa Al-Qumsani, A. Mirza, Amal A. Almostady, Jude Basrawi, S. Tabrez, Faiz Alsolami, Rami Al-Makki, Sami Al-Ghamdi, Abdullah Al-Ghamdi, Abdulnasser E. Alzahrani, Majda Altowairqi, Hassan Alharbi, M. Peace, M. Halwani, A. Al-Asmari
{"title":"Comparison between Blood, Non-Blood Fluids and Tissue Specimens for the Analysis of Cannabinoid Metabolites in Cannabis-Related Post-Mortem Cases","authors":"T. Zughaibi, Latifa Al-Qumsani, A. Mirza, Amal A. Almostady, Jude Basrawi, S. Tabrez, Faiz Alsolami, Rami Al-Makki, Sami Al-Ghamdi, Abdullah Al-Ghamdi, Abdulnasser E. Alzahrani, Majda Altowairqi, Hassan Alharbi, M. Peace, M. Halwani, A. Al-Asmari","doi":"10.3390/forensicsci3020025","DOIUrl":"https://doi.org/10.3390/forensicsci3020025","url":null,"abstract":"Cannabis use is widespread and is one of the most common drugs encountered in forensic-related analysis (antemortem and postmortem cases). However, the correlation between illicit cannabis use and death is rarely investigated, even while taking into consideration its role in the central nervous system depression and cardiovascular disorders. Few studies have discussed other non-blood specimens; this has brought a special interest in analyzing THC and its metabolites in different body parts in order to make precise forensic decisions. Herein, we are investigating the presence of Δ9-tetrahydrocannabinol (THC) and its metabolites:(11-hydroxy-Δ9-tetrahydrocannabinol (THC-OH) and 11-nor-Δ9- tetrahydrocannabinol-9-carboxy (THC-COOH)) in different postmortem specimens. Forty-three cases of bodily fluids and tissue post-mortem samples, previously found to be cannabinoid-positive were analyzed in the current investigation using alkaline hydrolysis followed by solid phase extraction and LC-MS/MS for THC and its metabolites concentration. In the current study, the highest median THC-COOH and THC-OH concentrations were detected in bile samples (1380 ng/mL and 8 ng/mL, respectively), while the highest THC median concentration was detected in gastric contents (48 ng/mL). This can be explained due to the postmortem distribution of blood to other bodily fluids and tissues and the accumulation in bile following multiple doses. Furthermore, high THC levels in gastric contents can be explained by the undergoing cycles of entero-hepatic circulation which resulted in a significant increase in THC in gastric contents. THC-COOH can be the best indicator to detect cannabinoids in toxicology studies, thus the inclusion of active THC metabolites is essential in death investigations. Additionally, THC-OH concentrations in postmortem cases could be influenced by body mass index. In this study, all types were specimens found to be suitable for testing cannabinoid metabolites, except for vitreous humor which showed low rates of detectability for cannabinoid metabolites.","PeriodicalId":45852,"journal":{"name":"Forensic Sciences Research","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82654934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"How to sample a seizure plant: the role of the visualization spatial distribution analysis of <i>Lophophora williamsii</i> as an example.","authors":"Jiaman Lin, Keming Yun, Qiran Sun, Ping Xiang, Lina Wu, Shuo Yang, Junling Dun, Shanlin Fu, Hang Chen","doi":"10.1093/fsr/owad014","DOIUrl":"10.1093/fsr/owad014","url":null,"abstract":"<p><p>Natural compounds in plants are often unevenly distributed, and determining the best sampling locations to obtain the most representative results is technically challenging. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) can provide the basis for formulating sampling guideline. For a succulent plant sample, ensuring the authenticity and <i>in situ</i> nature of the spatial distribution analysis results during MSI analysis also needs to be thoroughly considered. In this study, we developed a well-established and reliable MALDI-MSI method based on preservation methods, slice conditions, auxiliary matrices, and MALDI parameters to detect and visualize the spatial distribution of mescaline <i>in situ</i> in <i>Lophophora williamsii</i>. The MALDI-MSI results were validated using liquid chromatography-tandem mass spectrometry. Low-temperature storage at -80°C and drying of \"bookmarks\" were the appropriate storage methods for succulent plant samples and their flower samples, and cutting into 40 μm thick sections at -20°C using gelatin as the embedding medium is the appropriate sectioning method. The use of DCTB (trans-2-[3-(4-tert-butylphenyl)-2-methyl-2-propenylidene]malononitrile) as an auxiliary matrix and a laser intensity of 45 are favourable MALDI parameter conditions for mescaline analysis. The region of interest semi-quantitative analysis revealed that mescaline is concentrated in the epidermal tissues of <i>L. williamsii</i> as well as in the meristematic tissues of the crown. The study findings not only help to provide a basis for determining the best sampling locations for mescaline in <i>L. williamsii</i>, but they also provide a reference for the optimization of storage and preparation conditions for raw plant organs before MALDI detection.</p><p><strong>Key points: </strong>An accurate <i>in situ</i> MSI method for fresh water-rich succulent plants was obtained based on multi-parameter comparative experiments.Spatial imaging analysis of mescaline in <i>Lophophora williamsii</i> was performed using the above method.Based on the above results and previous results, a sampling proposal for forensic medicine practice is tentatively proposed.</p>","PeriodicalId":45852,"journal":{"name":"Forensic Sciences Research","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/50/7f/owad014.PMC10445667.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10262458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Stéphane Sauvagère, Amaury Pussiau, Sylvain Hubac, Audrey Gouello, Alexandre Poussard, J. Lavigne, Amel Larnane, C. Siatka, Francis Hermitte
{"title":"Innovations in Forensic Sciences for Human Identification by DNA in the French Gendarmerie during the Last 10 Years","authors":"Stéphane Sauvagère, Amaury Pussiau, Sylvain Hubac, Audrey Gouello, Alexandre Poussard, J. Lavigne, Amel Larnane, C. Siatka, Francis Hermitte","doi":"10.3390/forensicsci3020024","DOIUrl":"https://doi.org/10.3390/forensicsci3020024","url":null,"abstract":"The IRCGN (Institut de Recherche Criminelle de la Gendarmerie Nationale) is a forensic science institute built by the French Gendarmerie which has the ability to exploit crime scene evidence. Any piece of evidence, anywhere in the world, in any environment, can be examined by IRCGN teams deployed in just a few hours. During the past 10 years, experts specializing in genetics have developed innovative genetic engineering technologies for application in forensic sciences. In this review, we highlight the main innovations and the creation of new tools for human identification, which are fully suited to the French Gendarmerie’s needs. Devices developed by the IRCGN are specific to the Gendarmerie’s purposes.","PeriodicalId":45852,"journal":{"name":"Forensic Sciences Research","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83118480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparison of Derivatization Methods for Groomed Latent Print Residues Analysis via Gas Chromatography","authors":"Jessica Kindell, Candice M. Bridge","doi":"10.3390/forensicsci3020023","DOIUrl":"https://doi.org/10.3390/forensicsci3020023","url":null,"abstract":"The practice of latent print analysis is comprised of a visual examination and the comparison of the fingerprint pattern from a questioned print to an exemplar(s). When a questioned print is either smudged or contains little pattern detail, the print comparison would be considered an inconclusive determination. However, in these scenarios, the latent print residues (LPRs) could provide associative information to supplement the current ACE-V (Analysis, Comparison, Examination-Verification) process. Advancements using analytical techniques allow for the analysis of LPR chemistry; however, derivatization is generally required to increase the abundance of components not traditionally observed in gas chromatography. This study aimed to determine whether two derivatization reagents, boron trifluoride in methanol (BF3-MeOH) and N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA), provide a better recovery of LPR components from a porous or non-porous substrate. Five volunteers deposited groomed latent print samples onto two substrates: a microfiber filter (porous) and a microscope slide (non-porous). The residues were derivatized or evaporated prior to the gas chromatography-mass spectrometry (GC-MS) analysis. The percent recoveries were higher, >83%, in the DCM extracted samples for both substrates compared to those samples prepared in hexanes. DCM/MSTFA derivatization provided the recovery of fatty acids that ranged from 20 to 30% for both substrates and a recovery of squalene at a rate of 2.37% for the filter sample and 4.2% for the slide sample. These rates were higher than the recovery rates obtained for the hexanes/BF3-MeOH-derivatized samples, with a range of 1–8% for the fatty acids recovery rates and 0.6–0.85% for squalene from both substrates. Overall, the MSTFA derivatization reagent produced higher recoveries for LPR on porous and non-porous substrates while providing a LPR chromatographic profile similar to that of a non-derivatized sample. The use of DCM as a solvent provided a wider range of LPR components recovered than hexanes and, thus, should be used as the extraction solvent when derivatizing samples, regardless of the substrate.","PeriodicalId":45852,"journal":{"name":"Forensic Sciences Research","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72905761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Angela Silva-Bessa, M. T. Ferreira, R. Dinis-Oliveira
{"title":"Among Bodies: Portuguese Cemeterial Exhumations Three Years after a Pandemic","authors":"Angela Silva-Bessa, M. T. Ferreira, R. Dinis-Oliveira","doi":"10.3390/forensicsci3020022","DOIUrl":"https://doi.org/10.3390/forensicsci3020022","url":null,"abstract":"On 19 March 2020, a “state of emergency” was declared in Portugal due to the manifestation of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), with an exponential rate of infection and high mortality and morbidity rates. Since then, the state of emergency was declared a further fifteen times until 30 April 2021, during which a total of 16,974 deaths associated with COVID-19 were acknowledged in the country. Over the aforementioned period, guidelines were followed regarding the handling of suspected cases in autopsy rooms, mortuaries, and cemeteries. However, no procedures have been established regarding the handling of human remains during and after cemeterial exhumations. Furthermore, little is known about the virus survival and its spatial distribution in postmortem human tissues. Given that the minimum Portuguese legal period of inhumation is ending and cemeteries have been facing limited burial space and soon will start exhuming buried individuals, the authors believe it is important to reflect on the matter.","PeriodicalId":45852,"journal":{"name":"Forensic Sciences Research","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81814368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. M. Barone, Rosa Maria Di Maggio, Silvia Mesturini
{"title":"A Complementary Remote-Sensing Method to Find Persons Missing in Water: Two Case Studies","authors":"P. M. Barone, Rosa Maria Di Maggio, Silvia Mesturini","doi":"10.3390/forensicsci3020021","DOIUrl":"https://doi.org/10.3390/forensicsci3020021","url":null,"abstract":"This short communication discusses how a specific geoarchaeological remote-sensing (RS) method, such as analyzing satellite images through NDWI (Normalized Difference Water Index), can be used to aid in searching and locating persons missing in watercourses. Thanks to its high capacity to analyze changes in the surface water area, this index can remotely detect the presence of anomalies related to disappearances in water bodies and provide valuable information that can reduce the use of human resources and help pinpoint likely areas of search. Two real-life cases of missing persons in rivers in which the NDWI index was used are presented, and the results obtained are discussed, emphasizing the importance of NDWI analysis as a complementary method to different approaches, especially non-invasive and remote-sensed ones, when positively searching for missing persons.","PeriodicalId":45852,"journal":{"name":"Forensic Sciences Research","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73552100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparing Traditional Age Estimation at the Defense POW/MIA Accounting Agency to Age Estimation Using Random Forest Regression","authors":"Kyle A. McCormick","doi":"10.3390/forensicsci3020020","DOIUrl":"https://doi.org/10.3390/forensicsci3020020","url":null,"abstract":"Age estimation from developmental traits is typically assessed in isolation, where an age range is derived from known individuals that exhibit that degree of fusion. There are no objective means for incorporating developmental evidence from multiple areas of the skeleton into one cohesive age estimate. This limitation is obvious in the casework at the Defense POW/MIA Accounting Agency (DPAA), where subjectivity is introduced into age estimates based on multiple age indictors. This holds true even when age is derived from one source, The 1957 study by McKern and Stewart). This study uses 388 individuals from the McKern and Stewart study and 41 individuals from the Battle of Tarawa and uses Random Forest Regression (RFR) to estimate an age interval using multiple age indicators. These RFR estimates are compared to age estimates from the Forensic Anthropology Reports (FARs). Overall, FAR age estimates are more accurate (92.7%) than those from the two RFR models (80.5% and 76.6%). This increase in accuracy comes at the cost of some precision (FARs average age interval of 8.1 years and RFR average age intervals of 6.3 and 6.4 years). The RFR models prefer age indicators with late fusion, such as the medial clavicle, and the pubic symphysis, which exhibit a combination of developmental and degenerative ages in morphology. Some avenues for further research are discussed.","PeriodicalId":45852,"journal":{"name":"Forensic Sciences Research","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80054817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}