Journal of Transplantation最新文献

{"title":"Does Rabbit Antithymocyte Globulin (Thymoglobuline®) Have a Role in Avoiding Delayed Graft Function in the Modern Era of Kidney Transplantation?","authors":"Lluís Guirado","doi":"10.1155/2018/4524837","DOIUrl":"https://doi.org/10.1155/2018/4524837","url":null,"abstract":"<p><p>Delayed graft function (DGF) increases the risk of graft loss by up to 40%, and recent developments in kidney donation have increased the risk of its occurrence. Lowering the risk of DGF, however, is challenging due to a complicated etiology in which ischemia-reperfusion injury (IRI) leads to acute tubular necrosis. Among various strategies explored, the choice of induction therapy is one consideration. Rabbit antithymocyte globulin (rATG [Thymoglobuline]) has complex immunomodulatory effects that are relevant to DGF. In addition to a rapid and profound T-cell depletion, rATG inhibits leukocyte migration and adhesion. Experimental studies of rATG have demonstrated attenuated IRI-related tissue damage in reperfused tissues, consistent with histological evidence from transplant recipients. Starting rATG intraoperatively instead of postoperatively can improve kidney graft function and reduce the incidence of DGF. rATG is effective in preventing acute rejection in kidney transplant recipients at high immunological risk, supporting delayed calcineurin inhibitor (CNI) introduction which protects the graft from early insults. A reduced rate of DGF has been reported with rATG (started intraoperatively) and delayed CNI therapy compared to IL-2RA induction with immediate CNI in patients at high immunological risk, but not in lower-risk patients. Overall, induction with rATG induction is the preferred choice for supporting delayed introduction of CNI therapy to avoid DGF in high-risk patients but shows no benefit versus IL-2RA in lower-risk individuals. Evidence is growing that intraoperative rATG ameliorates IRI, and it seems reasonable to routinely start rATG before reperfusion.</p>","PeriodicalId":45795,"journal":{"name":"Journal of Transplantation","volume":"2018 ","pages":"4524837"},"PeriodicalIF":2.5,"publicationDate":"2018-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/4524837","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36402419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ramadan Fasting in Kidney Transplant Recipients: A Single-Centre Retrospective Study.","authors":"Ihab A Ibrahim,&nbsp;Ehab A Hassan,&nbsp;Abdelrahman M Alkhan,&nbsp;Mohamed A Hussein,&nbsp;Ahmed F Alhabashi,&nbsp;Tariq Z Ali,&nbsp;Yasir Z Shah,&nbsp;Ibrahim S Alahmadi,&nbsp;Mohamed S Abdelsalam,&nbsp;Mohamed E Rashwan,&nbsp;Ammar Abdulbaki,&nbsp;Dieter C Broering,&nbsp;Hassan A Aleid","doi":"10.1155/2018/4890978","DOIUrl":"https://doi.org/10.1155/2018/4890978","url":null,"abstract":"<p><strong>Background: </strong>Fasting during the lunar month of Ramadan is mandatory to all healthy adult Muslims. Renal transplant recipients are often worried about the impact of fluid and electrolyte deprivation during fasting on the function of their allograft. We aimed to examine the effect of fasting Ramadan on the graft function in renal transplant recipients.</p><p><strong>Methods: </strong>This retrospective cohort study included patients who underwent kidney transplantation in our tertiary referral center. Baseline pre-Ramadan estimated glomerular filtration rate (eGFR), mean arterial pressure (MAP), and urinary protein excretion were compared to those during and after Ramadan within and between the fasting and non-fasting groups.</p><p><strong>Results: </strong>The study population included 280 kidney transplant recipients who chose to fast during the Ramadan month (June-July 2014) and 285 recipients who did not fast. In the fasting group, baseline eGFR did not change from that during or post-Ramadan (72.6 ± 23.7 versus 72.3 ± 24.5 mL/min/1.73 m2, <i>P</i> = 0.53; and 72.6 ± 23.7 versus 72 ± 23.2 mL/min/1.73 m2, <i>P</i> = 0.14, respectively). Compared to baseline, there were no significant differences between the fasting and the non-fasting groups in terms of mean percent changes in eGFR, MAP, and urinary protein excretion.</p><p><strong>Conclusion: </strong>Fasting during the month of Ramadan did not have significant adverse effects on renal allograft function.</p>","PeriodicalId":45795,"journal":{"name":"Journal of Transplantation","volume":"2018 ","pages":"4890978"},"PeriodicalIF":2.5,"publicationDate":"2018-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/4890978","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36284868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Renal Transplants from Older Deceased Donors: Use of Preimplantation Biopsy and Differential Allocation to Dual or Single Kidney Transplant according to Histological Score Has No Advantages over Allocation to Single Kidney Transplant by Simple Clinical Indication.","authors":"Costanza Casati, Valeriana Giuseppina Colombo, Marialuisa Perrino, Ornella Marina Rossetti, Marialuisa Querques, Alessandro Giacomoni, Agnese Binaggia, Giacomo Colussi","doi":"10.1155/2018/4141756","DOIUrl":"10.1155/2018/4141756","url":null,"abstract":"<p><strong>Background: </strong>Grafts from elderly donors (ECD) are increasingly allocated to single (SKT) or dual (DKT) kidney transplantation according to biopsy score. Indications and benefits of either procedure lack universal agreement.</p><p><strong>Methods: </strong>A total of 302 ECD-transplants in period from Jan 1, 2000, to Dec 31, 2015, were allocated to SKT (SKT_pre) on clinical grounds alone (before Dec 2010, pre-DKT era, <i>n</i> = 170) or according to a clinical-histological protocol (after Dec 2010, DKT era, <i>n</i> = 132) to DKT (<i>n</i> = 48), SKT biopsy-based protocol (\"high-risk\", SKT_hr, <i>n</i> = 51), or SKT clinically based protocol (\"low-risk\", SKT_lr, <i>n</i> = 33). Graft and patient survival were compared between the two periods and between different transplant categories.</p><p><strong>Results: </strong>Graft and overall survival in recipients from ECD in pre-DKT and DKT era did not differ (5-year graft survival 87.7% and 84.2%, resp.); equal survival in the 2 ECD periods was shown in both donor age ranges of 60-69 and >70-years, and in low-risk or high-risk ECD categories. Within the DKT protocol SKT_hr showed worst graft and overall survival in the 60-69 donor age range; DKT did not result in significantly better outcome than SKT from ECD in either era. One-year posttransplant creatinine clearance in recipients did not differ between any ECD transplant category. At 3 and 5 years after transplantation there were significantly higher total dialysis-free recipient life years from an equal donor number in the pre-DKT era than in the DKT protocol.</p><p><strong>Conclusions: </strong>Use of a biopsy-based protocol to allocate grafts from aged donors to SKT or DKT did not result in better short term graft survival than a clinically based protocol with allocation only to SKT and reduced overall recipient dialysis-free life years in time.</p>","PeriodicalId":45795,"journal":{"name":"Journal of Transplantation","volume":"2018 ","pages":"4141756"},"PeriodicalIF":2.5,"publicationDate":"2018-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5976897/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36189923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pilot Analysis of Late Conversion to Belatacept in Kidney Transplant Recipients for Biopsy-Proven Chronic Tacrolimus Toxicity.","authors":"Shruti Gupta, Ivy Rosales, David Wojciechowski","doi":"10.1155/2018/1968029","DOIUrl":"10.1155/2018/1968029","url":null,"abstract":"<p><strong>Background: </strong>Calcineurin inhibitors are associated with chronic nephrotoxicity, manifesting as interstitial fibrosis/tubular atrophy (IF/TA) and arteriolar hyalinosis. Conversion from tacrolimus to belatacept may be one strategy to preserve renal function.</p><p><strong>Methods: </strong>We conducted a retrospective review of renal transplant patients followed at our institution who were converted to belatacept and found to have chronic tacrolimus toxicity on biopsy. The primary outcome was eGFR at conversion as compared to eGFR at 3, 6, 12, and 24 months after conversion. We also assessed incidence of infection and rates of allograft survival at 1 year.</p><p><strong>Results: </strong>The average time between transplant and conversion was 11.9 years. There was no decrease in eGFR at any postconversion time point as compared with preconversion. The mean eGFR at time of preconversion was 32.9 mL/min, as compared with 35.6 mL/min at 3 months (<i>p</i> = 0.09), 34.1 mL/min at 6 months (<i>p</i> = 0.63), 34.9 mL/min at 12 months (<i>p</i> = 0.57), and 39.6 mL/min at 24 months after conversion (<i>p</i> = 0.92). Four of 7 patients had increases in their eGFR after conversion. All grafts were functioning at 1 year after conversion.</p><p><strong>Conclusion: </strong>While this study was limited by a small number of patients, belatacept conversion stabilized eGFR at all time points in patients with late allograft function due to chronic tacrolimus toxicity, with a trend towards increased eGFR at 3 months.</p>","PeriodicalId":45795,"journal":{"name":"Journal of Transplantation","volume":"2018 ","pages":"1968029"},"PeriodicalIF":2.5,"publicationDate":"2018-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/1968029","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36182731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Kinetics of Anti-HLA Antibodies in the First Year after Kidney Transplantation: In Whom and When Should They Be Monitored?","authors":"Maria Cristina Ribeiro de Castro,&nbsp;Erick A Barbosa,&nbsp;Renata P Souza,&nbsp;Fabiana Agena,&nbsp;Patrícia S de Souza,&nbsp;Gabriella Maciel,&nbsp;Hélcio Rodrigues,&nbsp;Nicolas Panajotopoulos,&nbsp;Daísa S David,&nbsp;Flávio J de Paula,&nbsp;Elias David-Neto","doi":"10.1155/2018/8316860","DOIUrl":"https://doi.org/10.1155/2018/8316860","url":null,"abstract":"<p><p>The impact of the kinetics of the anti-HLA antibodies after KTx on the occurrence of acute rejection as well as the better time-point to monitor anti-HLA Abs after transplantation is not completely defined. This prospective study followed 150 patients over 12 months after transplantation. Serum IgG anti-HLA Abs were detected by single antigen beads after typing donors and recipients for loci A, B, C, DR, and DQ. Before KTx, 89 patients did not present anti-HLA Abs and 2% developed \"de novo\" Abs during the 1st year, 39 patients were sensitized without DSAs, and 13% developed DSA after surgery; all of them presented ABMR. Sensitized patients presented higher acute rejection rates (36.4% versus 13.5%, <i>p</i> < 0.001), although 60% of the patients did not present ABMR. Patients, in whom DSA-MFI decreased during the first two weeks after surgery, did not develop ABMR. Those who sustained their levels presented a rate of 22% of ABMR. 85% of patients developed ABMR when MFIs increased early after transplantation (which occurred in 30% of the DSA positive patients). In the ABMR group, we observed an iDSA-MFI sharp drop on the fourth day and then an increase between the 7th and 14th POD, which suggests DSA should be monitored at this moment in sensitized patients for better ABMR prediction.</p>","PeriodicalId":45795,"journal":{"name":"Journal of Transplantation","volume":"2018 ","pages":"8316860"},"PeriodicalIF":2.5,"publicationDate":"2018-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/8316860","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36177855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preoperative Low-Density Lipoprotein Apheresis for Preventing Recurrence of Focal Segmental Glomerulosclerosis after Kidney Transplantation.","authors":"Akihito Sannomiya,&nbsp;Toru Murakami,&nbsp;Ichiro Koyama,&nbsp;Kosaku Nitta,&nbsp;Ichiro Nakajima,&nbsp;Shohei Fuchinoue","doi":"10.1155/2018/8926786","DOIUrl":"https://doi.org/10.1155/2018/8926786","url":null,"abstract":"<p><strong>Background: </strong>Focal segmental glomerulosclerosis (FSGS) often develops rapidly and frequently progresses to renal failure, while the recurrence rate after kidney transplantation is 20-50%. We performed low-density lipoprotein (LDL) apheresis before kidney transplantation in FSGS patients to prevent recurrence.</p><p><strong>Methods: </strong>Five adult patients with chronic renal failure due to FSGS undergoing living related donor kidney transplantation were investigated retrospectively. LDL apheresis was done 1-2 times before transplantation. Postoperative renal function and recurrence of FSGS were assessed.</p><p><strong>Results: </strong>The patients were two men and three women aged 24 to 41 years. The observation period ranged from 60 days to 22 months. Preoperative LDL apheresis was performed once in one patient and twice in four patients. Blood LDL cholesterol levels were normal before LDL apheresis and remained normal both after LDL apheresis and after kidney transplantation. Additional LDL apheresis was performed once in one patient with mild proteinuria after transplantation. The renal graft survived in all patients and there was no evidence of recurrent FSGS.</p><p><strong>Conclusions: </strong>Although the observation period was short, FSGS did not recur in all 5 patients receiving preoperative LDL apheresis. These results suggest that LDL apheresis can be effective in preventing recurrence of FSGS after kidney transplantation.</p>","PeriodicalId":45795,"journal":{"name":"Journal of Transplantation","volume":"2018 ","pages":"8926786"},"PeriodicalIF":2.5,"publicationDate":"2018-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/8926786","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36138550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Direct Antiviral Agents in Treatment of Chronic Hepatitis C Infection in Renal Transplant Recipients.","authors":"Sourabh Sharma, Debabrata Mukherjee, Ranjith K Nair, Bhaskar Datt, Ananth Rao","doi":"10.1155/2018/7579689","DOIUrl":"10.1155/2018/7579689","url":null,"abstract":"<p><strong>Background: </strong>Since the introduction of direct antiviral agents (DAAs), morbidity of HCV has considerably decreased but still no guidelines have been formulated in renal transplant recipients (RTRs). We studied efficacy and tolerability of direct antiviral agents in RTRs.</p><p><strong>Methods: </strong>This prospective observational study was conducted at Army Hospital Research & Referral, Delhi, from June 2016 to May 2017. Forty-five HCV infected RTRs with stable graft function were included.</p><p><strong>Results: </strong>Median time between renal transplantation and the start of anti-HCV therapy was 36 months (1-120 months). The majority (66.7%) were infected with genotype 3. Baseline median HCV RNA level was 542648 IU/ml (1189-55028534 IU/ml). Sofosbuvir-Ribavirin combination (24 weeks) was given to 30 patients including 3 cirrhotics, Ledipasvir-Sofosbuvir combination to 8 patients, and Daclatasvir-Sofosbuvir combination to 7 patients, including 2 cirrhotics. Rapid virological response was observed in 29 patients treated with Sofosbuvir/Ribavirin, all 8 patients on Sofosbuvir/Ledipasvir, and all 7 patients on Sofosbuvir/Daclatasvir. End treatment response and sustained virological response (12 weeks) were achieved in all patients irrespective of genotype or treatment regimen. Decrease in mean HCV RNA level and transaminase level was statistically significant (<i>p</i> < 0.01). Ribavirin was significantly associated with anaemia (<i>p</i> = 0.032).</p><p><strong>Conclusions: </strong>DAA regimens are well tolerated and highly efficacious. Response to DAA is good irrespective of genotype, drug combination, initial HCV RNA level, age or sex of patient, or graft age. However, Sofosbuvir/Ledipasvir and Sofosbuvir/Daclatasvir combination is preferable.</p>","PeriodicalId":45795,"journal":{"name":"Journal of Transplantation","volume":"2018 ","pages":"7579689"},"PeriodicalIF":2.5,"publicationDate":"2018-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5896212/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36127275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corticosteroid Weaning in Stable Heart Transplant Patients: Guidance by Serum Cortisol Level.","authors":"David A Baran,&nbsp;Cheryl Rosenfeld,&nbsp;Mark J Zucker","doi":"10.1155/2018/3740395","DOIUrl":"https://doi.org/10.1155/2018/3740395","url":null,"abstract":"<p><strong>Background: </strong>Despite earlier studies describing the feasibility of steroid weaning in heart transplant patients, the majority of patients are maintained on steroid therapy for life. We examined a strategy based on a single morning serum cortisol measurement.</p><p><strong>Methods: </strong>We assigned stable posttransplant patients, who were maintained on tacrolimus, mycophenolate mofetil, and corticosteroids, into one of two groups based on a screening morning cortisol level. Patients with a cortisol < 8 micrograms/deciliter were assigned to a \"maintenance\" group and the others were assigned to the weaning group and steroids were tapered off over 4-6 weeks. Patients were monitored on subsequent office visits for adrenal insufficiency and allograft rejection.</p><p><strong>Results: </strong>Thirty-one patients were enrolled (6 patients in the maintenance group and 25 in the steroid-weaning group). Mean follow-up was 10.2 ± 4 years for the weaning group and 9.0 ± 4.9 years in the maintenance group (<i>p</i> = 0.6). No cases of rejection were noted, nor did any patient resume steroid treatment following discontinuation.</p><p><strong>Conclusion: </strong>Steroids can be safely discontinued in stable heart transplant patients with an AM serum cortisol ≥ 8 micrograms/deciliter with appropriate outpatient follow-up. In this study, no patient suffered late rejection or clinically noted adrenal insufficiency.</p>","PeriodicalId":45795,"journal":{"name":"Journal of Transplantation","volume":"2018 ","pages":"3740395"},"PeriodicalIF":2.5,"publicationDate":"2018-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/3740395","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36024408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mending a Broken Heart: Treatment of Stress-Induced Heart Failure after Solid Organ Transplantation.","authors":"N Thao Galván,&nbsp;Kayla Kumm,&nbsp;Michael Kueht,&nbsp;Cindy P Ha,&nbsp;Dor Yoeli,&nbsp;Ronald T Cotton,&nbsp;Abbas Rana,&nbsp;Christine A O'Mahony,&nbsp;Glenn Halff,&nbsp;John A Goss","doi":"10.1155/2018/9739236","DOIUrl":"https://doi.org/10.1155/2018/9739236","url":null,"abstract":"<p><p>Stress-induced heart failure, also known as Broken Heart Syndrome or Takotsubo Syndrome, is a phenomenon characterized as rare but well described in the literature, with increasing incidence. While more commonly associated with postmenopausal women with psychiatric disorders, this entity is found in the postoperative patient. The nonischemic cardiogenic shock manifests as biventricular failure with significant decreases in ejection fraction and cardiac function. In a review of over 3000 kidney and liver transplantations over the course of 17 years within two transplant centers, we describe a series of 7 patients with Takotsubo Syndrome after solid organ transplantation. Furthermore, we describe a novel approach of successfully treating the transient, though potentially fatal, cardiogenic shock with a percutaneous ventricular assistance device in two liver transplant patients, while treating one kidney transplant patient medically and the remaining four liver transplant patients with an intra-aortic balloon pump. We describe our experience with Takotsubo's Syndrome and compare the three modalities of treatment and cardiac augmentation. Our series is novel in introducing the percutaneous ventricular assist device as a more minimally invasive intervention in treating nonischemic heart failure in the solid organ transplant patient, while serving as a comprehensive overview of treatment modalities for stress-induced heart failure.</p>","PeriodicalId":45795,"journal":{"name":"Journal of Transplantation","volume":"2018 ","pages":"9739236"},"PeriodicalIF":2.5,"publicationDate":"2018-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/9739236","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36024409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation of a Survey Questionnaire on Organ Donation: An Arabic World Scenario.","authors":"Rajvir Singh, Tulika Mehta Agarwal, Hassan Al-Thani, Yousuf Al Maslamani, Ayman El-Menyar","doi":"10.1155/2018/9309486","DOIUrl":"10.1155/2018/9309486","url":null,"abstract":"<p><strong>Objective: </strong>To validate a questionnaire for measuring factors influencing organ donation and transplant.</p><p><strong>Methods: </strong>The constructed questionnaire was based on the theory of planned behavior by Ajzen Icek and had 45 questions including general inquiry and demographic information. Four experts on the topic, Arabic culture, and the Arabic and English languages established content validity through review. It was quantified by content validity index (CVI). Construct validity was established by principal component analysis (PCA), whereas internal consistency was checked by Cronbach's Alpha and intraclass correlation coefficient (ICC). Statistical analysis was performed by SPSS 22.0 statistical package.</p><p><strong>Results: </strong>Content validity in the form of S-CVI/Average and S-CVI/UA was 0.95 and 0.82, respectively, suggesting adequate relevance content of the questionnaire. Factor analysis indicated that the construct validity for each domain (knowledge, attitudes, beliefs, and intention) was 65%, 71%, 77%, and 70%, respectively. Cronbach's Alpha and ICC coefficients were 0.90, 0.67, 0.75, and 0.74 and 0.82, 0.58, 0.61, and 0.74, respectively, for the domains.</p><p><strong>Conclusion: </strong>The questionnaire consists of 39 items on knowledge, attitudes, beliefs, and intention domains which is valid and reliable tool to use for organ donation and transplant survey.</p>","PeriodicalId":45795,"journal":{"name":"Journal of Transplantation","volume":"2018 ","pages":"9309486"},"PeriodicalIF":2.5,"publicationDate":"2018-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5822804/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35956765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}