NOTES AND QUERIES最新文献_第10页

Modular Cloning by Golden Gate Assembly and Possible Application in Pathway Design. 金门组装模块化克隆及其在通路设计中的应用。

IF 1.2 3区文学

NOTES AND QUERIES Pub Date : 2023-06-28 DOI: 10.2533/chimia.2023.437

Zrinka Raguz Nakic, Christin Peters

{"title":"Modular Cloning by Golden Gate Assembly and Possible Application in Pathway Design.","authors":"Zrinka Raguz Nakic, Christin Peters","doi":"10.2533/chimia.2023.437","DOIUrl":"10.2533/chimia.2023.437","url":null,"abstract":"Preparation of expression vectors using conventional cloning strategies is laborious and not suitable for the design of metabolic pathways or enzyme cascades, which usually requires the preparation of a vector library to identify productive clones. Recently, Modular Cloning as a novel cloning technique in synthetic biology has been developed. Modular Cloning relies on Golden Gate assembly and supports preparation of individual expression vectors in one-step and one-pot reactions, thus allowing rapid generation of vector libraries. A number of Modular Cloning toolkits for specific applications has been established, providing a collection of distinct genetic elements such as promoters, ribosome binding sites and tags, that can be combined individually in one-step using defined fusion sites. Modular Cloning has been successfully applied to generate various strains for producing value-added compounds. This was achieved by orchestrating complex pathways involving up to 20 enzymes. Due to the novelty of the genetic approach, industrial applications are still rare. In addition, some applications are limited due to the lack of high-throughput screening methods. This shifts the bottleneck from library preparation to screening capacity and needs to be addressed by future developments to pave the path for the establishment of Modular Cloning in industrial applications.","PeriodicalId":45476,"journal":{"name":"NOTES AND QUERIES","volume":"12 1","pages":"437-441"},"PeriodicalIF":1.2,"publicationDate":"2023-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82888450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"文学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Euripides’ Bacchae and Shakespeare’s Theseus in a Midsummer Night’s Dream 欧里庇得斯的《酒神》与莎士比亚的《仲夏夜之梦》

IF 0.1 3区文学

NOTES AND QUERIES Pub Date : 2023-06-28 DOI: 10.1093/notesj/gjad044

B. Sokol

引用次数: 0

A Potential Inspiration for Dracula’s R. M. Renfield 德古拉的r·m·伦菲尔德的潜在灵感

IF 0.1 3区文学

NOTES AND QUERIES Pub Date : 2023-06-26 DOI: 10.1093/notesj/gjad046

L. Davydov

引用次数: 0

Fear of COVID-19 and future anxiety among Polish university students during a pandemic. 大流行病期间波兰大学生对 COVID-19 的恐惧和对未来的焦虑。

IF 2.2 3区文学

NOTES AND QUERIES Pub Date : 2023-06-26 eCollection Date: 2023-01-01 DOI: 10.5114/hpr/165874

Monika Guszkowska, Anna Bodasińska

{"title":"Fear of COVID-19 and future anxiety among Polish university students during a pandemic.","authors":"Monika Guszkowska, Anna Bodasińska","doi":"10.5114/hpr/165874","DOIUrl":"10.5114/hpr/165874","url":null,"abstract":"Background: The pandemic poses a threat to human health and life, and anxiety is the most common psychological problem occurring during the COVID-19 pandemic. The aim of the study was to establish the differences in the fear of COVID-19 infection and the future anxiety experienced by students during the pandemic by gender, field of study, place of study and paid employment, and their relationships to psychological well-being.Participants and procedure: The study involved 1294 university students aged 18-30. The Future Anxiety Scale and short version of the Psychological General Well-Being Index were used. In addition, students stated whether they were concerned about the possibility of contracting the coronavirus and falling ill with COVID-19.Results: One-third of respondents stated that they felt fear of contracting the coronavirus. Women experienced a significantly stronger fear of COVID-19 and future anxiety than men. The observed interaction effects show that female students of medical faculties experienced a higher fear of COVID-19 than women studying fields of study related to physical activity and working students experienced a slightly higher future anxiety. A positive, but weak correlation between fear of COVID-19 and future anxiety was found. Psychological well-being correlated poorly with fear of COVID-19 and moderately with future anxiety.Conclusions: Because future anxiety was stronger in women, medical students, some master's students and working people, preventive measures should be taken to prevent excessive anxiety and to promote mental health in these groups.","PeriodicalId":45476,"journal":{"name":"NOTES AND QUERIES","volume":"1 1","pages":"252-261"},"PeriodicalIF":2.2,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10670792/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83147896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"文学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Finding (Or Not) an Educator for a Prince—On the Recent Discovery of a Missing Letter from Leonhard Euler 为王子寻找（或不寻找）教育家——论最近发现的一封遗失的欧拉信件

IF 0.1 3区文学

NOTES AND QUERIES Pub Date : 2023-06-23 DOI: 10.1093/notesj/gjad045

P. Beeley, A. Kleinert

引用次数: 0

Twelfth Night’s Moai: Le Solution Pour Maria’s ‘Fustian Riddle’ 第十二夜的Moai：为玛丽亚的“扶桑之谜”找到解决方案

IF 0.1 3区文学

NOTES AND QUERIES Pub Date : 2023-06-23 DOI: 10.1093/notesj/gjad043

Brett D. Jones

引用次数: 0

An Early Printing of an Anonymous Tract by Francis Bacon 弗朗西斯·培根《佚名小册子》的早期印本

3区文学

NOTES AND QUERIES Pub Date : 2023-06-21 DOI: 10.1093/notesj/gjad038

Alan Stewart

引用次数: 0

Andrew Bond: Surgeon Imprisoned in Paris with Thomas Paine 安德鲁·邦德:和托马斯·潘恩一起被囚禁在巴黎的外科医生

3区文学

NOTES AND QUERIES Pub Date : 2023-06-21 DOI: 10.1093/notesj/gjad041

Max Cooper

引用次数: 0

Monitoring of antibiotic use in broiler turkey flocks in the Warmia and Mazury province in 2019-2021. 2019-2021 年沃尔米亚省和马祖里省肉用火鸡群抗生素使用情况监测。

IF 2.7 3区文学

NOTES AND QUERIES Pub Date : 2023-06-16 eCollection Date: 2023-06-01 DOI: 10.2478/jvetres-2023-0032

Marcin Śmialek, Teresa Konieczka, Paweł Konieczka, Joanna Kowalczyk, Andrzej Koncicki, Krzysztof Kozłowski, Jan Jankowski

{"title":"Monitoring of antibiotic use in broiler turkey flocks in the Warmia and Mazury province in 2019-2021.","authors":"Marcin Śmialek, Teresa Konieczka, Paweł Konieczka, Joanna Kowalczyk, Andrzej Koncicki, Krzysztof Kozłowski, Jan Jankowski","doi":"10.2478/jvetres-2023-0032","DOIUrl":"10.2478/jvetres-2023-0032","url":null,"abstract":"Introduction: The increasing resistance of bacteria to antibiotics has obliged the EU Member States to reduce by 50% the use of antibiotics in animal production by 2030. This study was undertaken with the aim to analyse the use of antibiotics in flocks of broiler turkeys reared in the Warmia and Mazury province in a two-year period.Material and methods: From data from 238 production records of turkey flocks provided by the County Veterinary Inspectorates, the use of antibiotics (mg/kg) was analysed in turkey flocks reared in 2019-2021. The data provided the year of rearing, turkey sex and immunoprophylactic measures.Results: A significant decrease in antimicrobial use was reported in the male turkey flocks in 2021 (157 mg/kg body weight) in comparison to 2020 (241 mg) and 2019 (299 mg). In both male and female turkeys, the use of antimicrobials gradually decreased from 2019 to 2021. Significantly lower antibiotic use was reported in turkey flocks using autogenous vaccines.Conclusion: The positive trend shown in this study proves the possibility of meeting the EU recommendations for 50% reduction in the use of antibiotics in animal production by 2030. More emphasis should be placed on minimising the risk of infectious diseases requiring antibiotic therapy with welfare, biosecurity, immunomodulation and specific prophylaxis measures.","PeriodicalId":45476,"journal":{"name":"NOTES AND QUERIES","volume":"1 1","pages":"243-249"},"PeriodicalIF":2.7,"publicationDate":"2023-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10740319/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83127512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"文学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Locrine and Robert Greene’s Dramatic Canon 洛克林与罗伯特·格林的戏剧经典

IF 0.1 3区文学

NOTES AND QUERIES Pub Date : 2023-06-02 DOI: 10.1093/notesj/gjad039

M. Jackson

引用次数: 0