Reports of Biochemistry and Molecular Biology最新文献

{"title":"Expression Level of lncRNA CYTOR in Iranian Cervical Cancer Patients.","authors":"Ali Rajabi,&nbsp;Narges Dastmalchi,&nbsp;Neda Shokri,&nbsp;Samaneh Tayefeh-Gholami,&nbsp;Seyyed Mohammad Yaghoubi,&nbsp;Reza Safaralizadeh","doi":"10.52547/rbmb.12.1.120","DOIUrl":"10.52547/rbmb.12.1.120","url":null,"abstract":"<p><strong>Background: </strong>A critical role has been known for lncRNAs in the initiation and development of cancers. Therefore, lncRNAs have been reported as the possible biomarkers in relation to the diagnosis and therapy of malignancies. This project examined the change in CYTOR lncRNA expression in human cervical cancer samples as compared with adjacent healthy ones.</p><p><strong>Methods: </strong>We provided one hundred fifteen pairs of tumorous and adjacent healthy tissue specimens of cervical cancer patients. RNAs were isolated from tissue specimens and cDNAs were synthesized. We considered quantitative Real-time PCR (qRT-PCR) to examine the expression levels of CYTOR lncRNA. In addition, the biomarker activity of CYTOR and the associations between the lncRNA and clinicopathological characteristics were evaluated.</p><p><strong>Results: </strong>The significant increased expression of CYTOR was obtained in cancerous samples as compared with non-cancerous ones (P< 0.0001). A significant correlation was indicated between CYTOR expression and the squamous subtype of cervical cancer (p=0.046). The receiver operating characteristic (ROC) curve-related AUC (area under the curve), specificity, and sensitivity were calculated 0.88, 81.74%, and 80%, respectively, which may introduce CYTOR as a potential biomarker.</p><p><strong>Conclusion: </strong>CYTOR may be an effective oncogene and biomarker in cervical cancer cases given its increased expression in human cervical cancer tissues.</p>","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"12 1","pages":"120-126"},"PeriodicalIF":1.7,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10505465/pdf/rbmb-12-120.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10675077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Cancer Effect of <i>Dorema Ammoniacum Gum</i> by Targeting Metabolic Reprogramming by Regulating <i>APC, P53, KRAS</i> Gene Expression in HT-29 Human Colon Cancer Cells.","authors":"Elham Ghodousi-Dehnavi,&nbsp;Mohammad Arjmand,&nbsp;Ziba Akbari,&nbsp;Mansour Aminzadeh,&nbsp;Reza Haji Hosseini","doi":"10.52547/rbmb.12.1.127","DOIUrl":"10.52547/rbmb.12.1.127","url":null,"abstract":"<p><strong>Background: </strong>Colorectal cancer is a heterogeneous disease that leads to metabolic disorders due to multiple upstream genetic and molecular changes and interactions. The development of new therapies, especially herbal medicines, has received much global attention. <i>Dorema ammoniacum</i> is a medicinal plant. Its gum is used in healing known ailments. Studying metabolome profiles based on nuclear magnetic resonance 1HNMR as a non-invasive and reproducible tool can identify metabolic changes as a reflection of intracellular fluxes, especially in drug responses. This study aimed to investigate the anti-cancer effects of different gum extracts on metabolic changes and their impact on gene expression in HT-29 cell.</p><p><strong>Methods: </strong>Extraction of <i>Dorema ammoniacum</i> gum with hexane, chloroform, and dichloromethane organic solvents was performed. Cell inhibition growth percentage and IC₅₀ were assessed. Following treating the cells with dichloromethane extract, <i>p53, APC</i>, and <i>KRAS</i> gene expression were determined. 1HNMR spectroscopy was conducted. Eventually, systems biology software tools interpreted combined metabolites and genes simultaneously.</p><p><strong>Results: </strong>The lowest determined IC₅₀ concentration was related to dichloromethane solvent, and the highest was hexane and chloroform. The expression of the <i>KRAS</i> oncogene gene decreased significantly after treatment with dichloromethane extract compared to the control group, and the expression of tumor suppressor gene <i>p53</i> and <i>APC</i> increased significantly. Most gene-altered convergent metabolic phenotypes.</p><p><strong>Conclusion: </strong>This study's results indicate that the dichloromethane solvent of <i>Dorema ammoniacum</i> gum exhibits its antitumor properties by altering the expression of genes involved in HT-29 cells and the consequent change in downstream metabolic reprogramming.</p>","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"12 1","pages":"127-135"},"PeriodicalIF":1.7,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10505474/pdf/rbmb-12-127.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10302574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Cannabinoid Type 2 Receptor Activation in Renal Fibrosis Induced by Unilateral Ureteric Obstruction in Rats.","authors":"Mahmoud El Tohamy,&nbsp;Mohamed Adel,&nbsp;Fayza Rashad El-Menabawy,&nbsp;Gad El Mawla Gad,&nbsp;Randa El-Gamal,&nbsp;Hanaa El Serougy","doi":"10.52547/rbmb.12.1.59","DOIUrl":"10.52547/rbmb.12.1.59","url":null,"abstract":"<p><strong>Background: </strong>Chronic kidney disease (CKD) ends mostly with renal fibrosis. The effect of CB2 receptor on renal fibrosis has been unclear. The aim of this study was to investigate the effect of CB2 receptor on renal fibrosis and the mechanisms behind it.</p><p><strong>Methods: </strong>50 adult male Sprague-Dawley rats were divided into 5 groups; normal, sham; rats had their ureters only manipulated, UUO; rats had their left ureters ligated, and JWH post; rats had their left ureters ligated and they received JWH 133 for 14 days, JWH pre+post; rats received JWH 133 for 14 days before and after UUO procedure. Serum creatinine and BUN were assessed together with tissue MDA, GSH, and catalase. Histopathological evaluation of the renal tissue by H&E and Masson's trichrome was done. Immunohistochemical staining for TGF-β1, AQP1, Caspase-3, LC3B and p62 was performed. AQP1 and CB2 receptors genes expression was detected by quantitative RT-PCR.</p><p><strong>Results: </strong>UUO had caused severe damage in the renal tissue with reduction of the renal function parameter accompanied by increase in the collagen deposition with increase TGF-β1 and decrease AQP1 expression.</p><p><strong>Conclusions: </strong>The improvement of these parameters with JWH-133 suggests an anti-fibrotic role of CB2 receptor activation through reduction of oxidative stress, apoptosis, and autophagy.</p>","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"12 1","pages":"59-73"},"PeriodicalIF":1.7,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10505471/pdf/rbmb-12-59.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10311389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RT-qPCR Analysis of LAMP3 (CD208) Gene Expression in Oral Lichen Planus and Oral Squamous Cell Carcinoma.","authors":"Farnaz Mohajertehran,&nbsp;Nooshin Mohtasham,&nbsp;Mojtaba Ahmadi,&nbsp;Mehdi Shahabinejad,&nbsp;Maryam Mohammadi","doi":"10.52547/rbmb.12.1.36","DOIUrl":"10.52547/rbmb.12.1.36","url":null,"abstract":"<p><strong>Background: </strong>Many new studies have been conducted on cellular proteins to use them as prognostic markers or in target therapy through determining the increase or decrease in their expression in the lichen planus and OSCC. LAMP3 protein is one of these proteins which has been recently considered. Thus, considering the unknown etiology of lichen planus, significance of their early diagnosis and treatment and lack of a suitable and final treatment for this disease and oral cancers, and preventing the progression of lichen planus, which can turn into OSCC, we decided to investigate the level of expression of this gene and its effect on the progression, study the connection between these two conditions and the probable factors contributing to their etiopathogenesis.</p><p><strong>Methods: </strong>In this study, ninety-four paraffin blocks tissue samples of patients were obtained together with their demographic documents. LAMP3 expression was measured RT-qPCR method.</p><p><strong>Results: </strong>The results show that there is not any significant difference between age and sex population of our study. in squamous cell carcinoma the amount of expression of LAMP3 was higher than lichen planus and healthy margin. Average LAMP3 Gene expression in grade III was higher than group grade I & II in which considering significant level of 5%, it is statistically significant.</p><p><strong>Conclusions: </strong>According to the findings of this study, it can be concluded that the expression of the LAMP3 gene in SCC lesions is higher than in healthy tissue. Hence, LAMP3 gene expression can be used as a diagnostic biomarker.</p>","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"12 1","pages":"36-41"},"PeriodicalIF":1.7,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10505466/pdf/rbmb-12-36.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10311391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of lncRNA MEG3 Rs7158663 Polymorphism and Serum Expression with Colorectal Cancer in Egyptian Patients.","authors":"Mona Elhelaly Elsherbeny,&nbsp;Alyaa Ramadan Elsergany,&nbsp;Olfat Gamil Shaker","doi":"10.52547/rbmb.12.1.102","DOIUrl":"10.52547/rbmb.12.1.102","url":null,"abstract":"<p><strong>Background: </strong>Colorectal cancer (CRC) is considered the third most common cancer around the world and second in terms of mortality. A significant aspect in its development is genetics. The risk of CRC and other clinicopathologic characteristics were investigated in this work in relation to the long non-coding RNA (lncRNA) MEG3 rs7158663 polymorphism, MEG3 expression, in an Egyptian population.</p><p><strong>Methods: </strong>160 CRC patients and 160 healthy controls were enrolled in this case-control study. The lncRNA MEG3 rs7158663 was examined using TaqMan Real-time PCR. RT-PCR was used to assess the levels of serum MEG3 expression.</p><p><strong>Results: </strong>A significant higher expression of 'A' allele (risk allele) and A/A genotype in CRC cases vs. control subjects (P <0.001) Participants with A/A genotype had 4.8 times higher odds to exhibit CRC. Serum MEG3 gene expression was generally low in CRC patients, and it was considerably lower in those with the rs7158663 AA genotype than those with the GG genotype (P <0.001). It was found that CRC patients with the rs7158663 GA genotype had lower serum MEG3 expression levels than those with the GG genotype (P <0.001).</p><p><strong>Conclusions: </strong>MEG3 low expression and MEG3 rs7158663 (AA) were associated with CRC risk in Egyptian patients and may serve as a diagnostic and prognostic marker for CRC patients.</p>","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"12 1","pages":"102-111"},"PeriodicalIF":1.7,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10505460/pdf/rbmb-12-102.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10302576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protein Expression and Purification of Romiplostim and Analysis of Its Secretory Production Using an <i>In Silico</i> Investigated Signal Peptide in <i>E. Coli</i>.","authors":"Masoud Hashemzaei,&nbsp;Manica Negahdaripour,&nbsp;Reza Heidari,&nbsp;Mohammad Bagher Ghoshoon","doi":"10.52547/rbmb.12.1.27","DOIUrl":"10.52547/rbmb.12.1.27","url":null,"abstract":"<p><strong>Background: </strong>Romiplostim is a thrombopoietin receptor agonist approved for the treatment of immune thrombocytopenia. It is produced by recombinant DNA technology in <i>Escherichia coli</i>. Many researchers have studied the periplasmic or extracellular production of recombinant proteins in <i>E. coli</i> by using signal peptide sequences due to its advantages compared to intracellular production. In this study, the effect of the pelB signal peptide on Romiplostim production was analyzed.</p><p><strong>Methods: </strong>The nucleotide sequence of Romiplostim was codon optimized for expression in <i>E. coli</i> BL21. For analysis of the effect of the pelB signal peptide, pET-22b (+) and pET-15b plasmids were used. The probability of signal peptide cleavage and pathway was predicted by using the SignalP 5.0 program, and expression, purification, and biological activity of the recombinant protein were analyzed.</p><p><strong>Results: </strong><i>In-silico</i> analysis predicted the correct cleavage of the pelB signal peptide. However, the experimental results showed intracellular accumulation of the protein in fusion with this signal peptide without any detectable protein band in periplasmic or extracellular spaces. The <i>in-vivo</i> experiment of purified protein without signal peptide exhibited a significant increment in platelets compared to the control group.</p><p><strong>Conclusions: </strong>Romiplostim was expressed in <i>E. coli</i> with and without signal peptide. The latest one showed suitable <i>in-vivo</i> bioactivity. Despite the results of <i>in-silico</i> prediction, the pelB signal peptide could not transport the protein into the periplasm or extracellular environment in the experimental condition. Trying different signal peptides and more <i>in-silico</i> analysis might be helpful for the efficient secretion of the Romiplostim protein.</p>","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"12 1","pages":"27-35"},"PeriodicalIF":1.7,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10505470/pdf/rbmb-12-27.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10309471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulatory Role of circRNA-0067835 in Behcet Disease through Targeting Micro RNA-155: Implication of <i>ATG1, AKT</i> and <i>MTOR</i>.","authors":"Shimaa Saad El-Din,&nbsp;Laila Ahmed Rashed,&nbsp;Doaa Saeed Mohamed,&nbsp;Mervat Eissa,&nbsp;Reham Mohammad Raafat Hamed,&nbsp;Rania Elsayed Hussein","doi":"10.52547/rbmb.12.1.195","DOIUrl":"10.52547/rbmb.12.1.195","url":null,"abstract":"<p><strong>Background: </strong>Autophagy has been proven to contribute to maintaining eukaryotic cells' normal intracellular homeostasis, whereas autophagy malfunction may predispose to Behcet Disease (BD). The accumulation of the products of autophagic degradation as well as impairment in autophagic flux in cases with BD, may be attributed to dysregulated miRNA-155 expression. This study attempts to determine the contribution of circRNA-0067835 in miRNA-155-mediated modulation of the autophagy axis as well as to investigate its impact on the production of pro-inflammatory cytokines in BD.</p><p><strong>Methods: </strong>This study was carried out on 40 cases with BD and 40 healthy control subjects. The collection of serum samples was done before performing a real-time PCR to estimate the relative gene expression of ATG1, AKT, miRNA-155, mTOR, TAB2, and circRNA-0067835. Additionally, IL-1β, IL-17, and TNF-α serum levels were determined by ELISA.</p><p><strong>Results: </strong>Behcet Disease (BD) patients had significantly upregulated circRNA-0067835, with subsequent downregulation of its target gene, miRNA-155 than controls (P<0.05). In addition, decreased miRNA-155 gene expression was correlated with significantly increased TAB2 gene expression levels in BD patients compared to the controls (P<0.05). Furthermore, enhanced production of pro-inflammatory cytokines was detected in cases with BD than in controls.</p><p><strong>Conclusion: </strong>The correlation between circRNA-0067835 and miRNA-155 fairly contributes to the regulation of cytokine production in BD via the modulation of autophagy. The investigation of the circRNA-0067835 and the microRNA-155 and their downstream adaptor molecules could be a potential therapeutic agent for BD.</p>","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"12 1","pages":"195-204"},"PeriodicalIF":1.7,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10505462/pdf/rbmb-12-195.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10311390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bone Marrow-Derived Mesenchymal Stem Cells and Pioglitazone or Exendin-4 Synergistically Improve Insulin Resistance via Multiple Modulatory Mechanisms in High-Fat Diet/Streptozotocin-Induced Diabetes in Rats.","authors":"Mohamed Mesbah Mohamed,&nbsp;Laila Ahmed Rashed,&nbsp;Noha Ahmed El-Boghdady,&nbsp;Mahmoud Mohamed Said","doi":"10.52547/rbmb.12.1.42","DOIUrl":"10.52547/rbmb.12.1.42","url":null,"abstract":"<p><strong>Background: </strong>Diabetes mellitus (DM) is a metabolic disease, characterized by hyperglycemia resulting from defects in insulin secretion and/or insulin action. The current study was designed to assess the therapeutic potential of bone marrow-derived mesenchymal stem cells (BM-MSCs) alone and in combination with pioglitazone (Pz) or exendin-4 (Ex) in high-fat diet/streptozotocin (HFD/STZ)-induced diabetes in rats.</p><p><strong>Methods: </strong>The rats were subjected to the HFD for three weeks before being injected with a single low dosage of STZ (35 mg/kg bw). The animals were assigned to different treatment groups after type II diabetes mellitus (T2DM) induction was confirmed.</p><p><strong>Results: </strong>Severe insulin resistance was verified in untreated HFD/STZ T2DM rats, along with the exaggeration of oxidative stress, inflammation, apoptosis, and autophagy suppression in the adipose tissues. Monotherapy of HFD/T2DM rats with BM-MSCs and Pz or Ex alleviated diabetic complications by increasing insulin sensitivity, decreasing apoptosis and inflammation as evidenced by a decrease in serum tumor necrosis factor-alpha, caspase-3, and nuclear factor-kappa B (NF-κB) genes expression and Janus kinase (JNK) protein expression, and enhancing autophagy as revealed by upregulation in beclin and LC3, as well as peroxisome proliferator-activated receptor-γ coactivator-1 alpha (PGC-1α) genes expression in the adipose tissues. An augmented ameliorative efficacy was recorded in combined treatments. The biochemical and molecular results were confirmed by histological investigation of pancreatic tissues.</p><p><strong>Conclusions: </strong>Combining Pz or Ex with BM-MSCs is a synergistic therapeutic option that reduces insulin resistance and subsequent complications in T2DM via multiple molecular mechanisms.</p>","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"12 1","pages":"42-58"},"PeriodicalIF":1.7,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10505456/pdf/rbmb-12-42.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10302578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome Data Reveal Geographic Heterogeneity in Gene Expression in Patients with Prostate Cancer.","authors":"Yi Zheng,&nbsp;Yang Wang,&nbsp;Haitian He,&nbsp;Zhuping Zou,&nbsp;Huiling Lu,&nbsp;Jinlong Li,&nbsp;Jun Cai,&nbsp;Kebing Wang","doi":"10.52547/rbmb.12.1.92","DOIUrl":"10.52547/rbmb.12.1.92","url":null,"abstract":"<p><strong>Background: </strong>The incidence of prostate cancer (PC) exhibits geographical heterogeneity. However, the metabolic mechanisms underlying this geographic heterogeneity remain unclear. This study aimed to reveal the metabolic mechanism of the geographic heterogeneity in the incidence of PC.This study aimed to investigate the anti-cancer effects of different gum extracts on metabolic changes and their impact on gene expression in HT-29 cell.</p><p><strong>Methods: </strong>Transcriptomic data from public databases were obtained and analyzed to screen geographic-differentially expressed genes and metabolic pathways. Associations between these differentially expressed genes and the incidence of PC were determined to identify genes that were highly associated with PC incidence. A co-expression network analysis was performed to identify geographic-specific regulatory pathways.</p><p><strong>Results: </strong>A total of 175 differentially expressed genes were identified in four countries and were associated with the regulation of DNA replication and the metabolism of pyrimidine, nucleotides, purines, and galactose.Additionally, the expression of the genes <i>CLVS2</i>, <i>SCGB1A1</i>, <i>KCNK3</i>, <i>HHIPL2</i>, <i>MMP26</i>, <i>KCNJ15</i>, and <i>PNMT</i> was highly correlated with the incidence of PC. Geographic-specific differentially expressed genes in low-incidence areas were highly correlated with <i>KCNJ15</i>, <i>MMP26</i>, <i>KCNK3</i>, and <i>SCCB1A1</i>, which play a major role in ion channel-related functions.</p><p><strong>Conclusions: </strong>This study suggests that geographic heterogeneity in PC incidence is associated with the expression levels of genes associated with amino acid metabolism, lipid metabolism, and ion channels.</p>","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"12 1","pages":"92-101"},"PeriodicalIF":1.7,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10505463/pdf/rbmb-12-92.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10311386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of miR-18a-5p, miR-19a-3p, and miR-20a-5p on <i>In Vitro</i> Cardiomyocyte Differentiation of Human Endometrium Tissue-Derived Stem Cells Through Regulation of Smad4 Expression.","authors":"Behnaz Maleki,&nbsp;Mahdi Noureddini,&nbsp;Somayeh Saadat,&nbsp;Javad Verdi,&nbsp;Alireza Farrokhian,&nbsp;Hossein Ghanbarian,&nbsp;Ebrahim Cheraghi,&nbsp;Behrang Alani","doi":"10.52547/rbmb.12.1.136","DOIUrl":"10.52547/rbmb.12.1.136","url":null,"abstract":"<p><strong>Background: </strong>Smad4 regulates the expression of the genes required for heart homeostasis. Regarding the central role of microRNAs in cardiac biology, we investigated the expression of the three Smad4-targeting miRNAs, namely miR-18a-5p, miR-19a-3p, and miR-20a-5p, as well as Smad4 during differentiation of human endometrium-derived mesenchymal stem cells (hEMSCs) into cardiomyocytes (CMs).</p><p><strong>Methods: </strong>To evaluate mesenchymal phenotype and multi-lineage differentiation ability of hEMSCs, immunophenotyping by flow cytometry and differentiation into osteoblasts and adipocytes were performed, respectively. For transdifferentiation into CMs, hEMSCs were exposed to a cardiomyogenic medium composed of 5-aza and bFGF for 30 days. The comparison between transcriptional expression levels of Nkx2-5, GATA4, Smad4, TNNT2, TBX5, miR-18a-5p, miR-19a-3p, and miR-20a-5p by qRT-PCR, as well as protein levels of Nkx2-5, Smad4, and cTnT by immunofluorescence staining, was conducted in every 6 days.</p><p><strong>Results: </strong><i>In vitro</i>, the mesenchymal stem cell phenotype of hEMSCs and their potency for differentiation into other MSCs were confirmed. Differentiated hEMSCs had morphological characteristics of CMs. The percentage of positive cells for Nkx2-5, Smad4, and cTnT proteins was increased following induction and culminated on the 24th day. Also, mRNA levels of Nkx2-5, GATA4, Smad4, TNNT2, and TBX5 exhibited the same trend. The expression of investigated miRNAs was significantly decreased sequentially. A significant negative correlation between expressions of Smad4 and investigated miRNAs was observed.</p><p><strong>Conclusion: </strong>Our results indicate that miR-18a-5p, miR-19a-3p, and miR-20a-5p are involved in the cardiac differentiation propensity of hEMSCs potentially by regulation of Smad levels. Although, more mechanistic experiments are required to confirm this idea.</p>","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"12 1","pages":"136-146"},"PeriodicalIF":1.7,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10505458/pdf/rbmb-12-136.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10311388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}