DNA Barcodes最新文献

Ground-truthing phylotype assignments for Antarctic invertebrates 南极无脊椎动物的真实的种型分配

DNA Barcodes Pub Date : 2017-01-27 DOI: 10.1515/DNA-2017-0001

Paul Czechowski, L. Clarke, A. Cooper, M. Stevens

{"title":"Ground-truthing phylotype assignments for Antarctic invertebrates","authors":"Paul Czechowski, L. Clarke, A. Cooper, M. Stevens","doi":"10.1515/DNA-2017-0001","DOIUrl":"https://doi.org/10.1515/DNA-2017-0001","url":null,"abstract":"Biodiversity information from Antarctic terrestrial habitats helps conservation efforts, but the distribution and diversity particularly of microinvertebrates remains poorly understood. Springtails, mites, tardigrades, nematodes and rotifers are difficult to identify using morphological features, hence DNA-based metabarcoding methods are well suited for their study. We compared taxonomy assignments of a high throughput sequencing metabarcoding approach using one ribosomal DNA (18S rDNA) and one mitochondrial DNA (cytochrome c oxidase subunit I - COI) marker with morphological reference data. Specifically, we compared metabarcoding or morphological taxonomic assignments on multiple taxonomic levels in an artificial DNA blend containing Australian invertebrates, and in seven extracts of Antarctic soils containing known micro-faunal taxa. Avoiding arbitrary application of metabarcoding analysis parameters, we calibrated those parameters with metabarcoding data from non-Antarctic soils. Metabarcoding approaches employing 18S rDNA and COI markers enabled detection of small and cryptic Antarctic invertebrates, and on low taxonomic ranks 18S data outperformed COI data in this respect. Morphological taxonomy determination did not outperform metabarcoding approaches. Our study demonstrates how barcoding markers can be tested prior to their application to specific taxonomic groups, and that taxonomy fidelity of markers needs to be validated in relation to environment, taxa, and available reference information.","PeriodicalId":446240,"journal":{"name":"DNA Barcodes","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126657232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Barcoding, phylogeography and speciesboundaries in clownfishes of the Indian Ocean 印度洋小丑鱼的条形码、系统地理学和物种边界

DNA Barcodes Pub Date : 2015-05-27 DOI: 10.1515/DNA-2015-0002

K. Dhaneesh, T. T. A. Kumar, Appukuttan Nair Biju Kumar

引用次数: 6

Sequencing of COI gene in four rodent pests for species identification 四种啮齿动物COI基因序列测定及种类鉴定

DNA Barcodes Pub Date : 2015-01-30 DOI: 10.1515/DNA-2015-0001

R. Lakshminarayanan, Achiraman Shanmugam, Archunan Govindaraju

引用次数: 3

Estimating the effectiveness of species identification by sequencing of two chloroplast DNA loci (matK and rbcL) in selected groups of Polish flora 通过对波兰植物群中两个叶绿体DNA位点(matK和rbcL)的测序来估计物种鉴定的有效性

DNA Barcodes Pub Date : 2015-01-19 DOI: 10.1515/DNA-2015-0003

M. Combik, Z. Mirek

引用次数: 2

A successful case of DNA barcoding used in an international trade dispute DNA条形码在国际贸易争端中的成功案例

DNA Barcodes Pub Date : 2014-08-27 DOI: 10.2478/DNA-2014-0004

Chen Jian, Qiu Deyi, Yue Qiaoyun, H. Jia, Liu Dexing, Wei Xiaoya, Zheng Leiqing

引用次数: 5

DNA barcoding of the vegetable Leafminer Liriomyza sativae Blanchard (Diptera: Agromyzidae) in Bangladesh 孟加拉国蔬菜叶蚁Liriomyza sativae Blanchard的DNA条形码分析(双翅目:蛾科)

DNA Barcodes Pub Date : 2014-08-27 DOI: 10.2478/DNA-2014-0005

S. Amin, S. Scheffer, M. Lewis, M. K. Pasha, B. A. Bhuiya

引用次数: 7

Towards a DNA barcode library for Egyptian flora,with a preliminary focus on ornamental treesand shrubs of two major gardens 建立埃及植物的DNA条形码库，初步关注两个主要花园的观赏树木和灌木

DNA Barcodes Pub Date : 2013-09-06 DOI: 10.2478/DNA-2013-0004

H. Elansary

引用次数: 11

Taxonomic rank of Indian tortoise:revisit with DNA barcoding perspective 印度龟的分类等级:用DNA条形码的观点重新审视

DNA Barcodes Pub Date : 2013-05-06 DOI: 10.2478/DNA-2013-0003

S. Kundu, K. C. Das, S. Ghosh

{"title":"Taxonomic rank of Indian tortoise:revisit with DNA barcoding perspective","authors":"S. Kundu, K. C. Das, S. Ghosh","doi":"10.2478/DNA-2013-0003","DOIUrl":"https://doi.org/10.2478/DNA-2013-0003","url":null,"abstract":"Taxonomic rank of terrestrial tortoise inhabiting in India and adjacent countries has been an unresolved issue. Wild living species of Manouria and Indotestudo genera have been classiied through conventional taxonomy, but not adequately discriminated by mitochondrial cytochrome b gene. Cytochrome oxidase (COI) marker has been quite successful to achieve the exact species level information. There needs of an accurate sequence based effort for the extant northeast (NE) Indian tortoises to identify them accurately and to provide locality information. To estimate the Kimura-2-Parameter (K2P) divergences and to construct the NeighbourJoining (NJ) phylogeny, we generated six partial COI sequences derived from expert-identiied tortoise specimens. Both BLASTn and Bold-IDs revealed the deinitive identity of Manouria, whereas the congeners of Indotestudo remain inconclusive by only a two-fold interspeciic divergence gap with the other named Indian tortoise species. The NJ phylogeny readily differentiated the three Indian tortoise genera with their respective conspecies but depicted a nonsynonomous group of Indotestudo congeners. This study shows that the molecular identiication of Manouria emys phayrei, Indotestudo elongata and an allopatric population of Indotestudo forstenii in NE India corresponds correctly with existing morphological key support.","PeriodicalId":446240,"journal":{"name":"DNA Barcodes","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128907568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

DNA Barcoding:A tool for species identification from herbal juices DNA条形码:一种草本果汁物种鉴定工具

DNA Barcodes Pub Date : 2013-02-14 DOI: 10.2478/DNA-2013-0002

P. Mahadani, S. Ghosh

引用次数: 29

An integrated and validated DNA-based protocol developedto fight against commercial frauds – A case of fraudulentsubstitutions for deer products 为打击商业欺诈而开发的基于dna的综合验证协议-鹿产品欺诈性替代案例

DNA Barcodes Pub Date : 2013-02-14 DOI: 10.2478/DNA-2013-0001

W. M. Sin, Y. K. Tam, S. Tsui, C. Ng, Cs Mok, Wy Ha

引用次数: 7