DNA Barcodes最新文献

筛选
英文 中文
A case study of the forensic application of DNA Barcoding to sharkfin identification in the Mexican Pacific DNA条形码法证应用于墨西哥太平洋鲨鱼鉴定的案例研究
DNA Barcodes Pub Date : 1900-01-01 DOI: 10.1515/DNA-2015-0012
Héctor Espinosa, Christian Lambarri, Armando J. Martínez, Andrea Jiménez
{"title":"A case study of the forensic application of DNA Barcoding to sharkfin identification in the Mexican Pacific","authors":"Héctor Espinosa, Christian Lambarri, Armando J. Martínez, Andrea Jiménez","doi":"10.1515/DNA-2015-0012","DOIUrl":"https://doi.org/10.1515/DNA-2015-0012","url":null,"abstract":"","PeriodicalId":446240,"journal":{"name":"DNA Barcodes","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128986918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Fishing for barcodes in the Torrent: from COI to complete mitogenomes on NGS platforms 在洪流中寻找条形码:从COI到NGS平台上的完整有丝分裂基因组
DNA Barcodes Pub Date : 1900-01-01 DOI: 10.1515/DNA-2015-0019
D. Hinsinger, R. Debruyne, Maeva Thomas, G. Denys, M. Mennesson, Jose Utage, A. Dettai
{"title":"Fishing for barcodes in the Torrent: from COI to complete mitogenomes on NGS platforms","authors":"D. Hinsinger, R. Debruyne, Maeva Thomas, G. Denys, M. Mennesson, Jose Utage, A. Dettai","doi":"10.1515/DNA-2015-0019","DOIUrl":"https://doi.org/10.1515/DNA-2015-0019","url":null,"abstract":"samples, adaptable on any desktop NGS platform. It enables to extend from the prevalent barcoding approach by shifting from the single COI to complete mitogenome sequencing. Abstract: The adoption of Next-Generation Sequencing by the fit between the classical COI barcode and the Sanger-based sequencing method. Here we describe a framework for the sequencing and multiplexing of mitogenomes on NGS platforms that implements (I) a universal long-range PCR-based amplification technique, (II) a two-level multiplexing approach ( i.e. divergence-based and specific tag indexing), and (III) a dedicated demultiplexing and assembling script from an Ion Torrent sequencing platform. We provide a case study of mitogenomes obtained for two vouchered individuals of daces Leuciscus burdigalensis and L. oxyrrhis and show that this workflow enables to recover over 100 mitogenomes per sequencing chip on a PGM sequencer, bringing the individual cost down below 7,50€ per mitogenome (as of current 2015 sequencing costs). The use of several kilobases for identification purposes, as involved in the improved DNA-barcode we propose, stress the need for data reliability, especially through metadata. Based on both scientific and economic considerations, this framework presents a relevant approach for multiplexing","PeriodicalId":446240,"journal":{"name":"DNA Barcodes","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130795754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 29
DNA Barcoding of Red Sea Fishes from Saudi Arabia – The first approach 来自沙特阿拉伯红海鱼类的DNA条形码-第一种方法
DNA Barcodes Pub Date : 1900-01-01 DOI: 10.2478/DNA-2014-0003
Subrata Trivedi, Raafat Affan, A. Alessa, A. Ansari, Bishal Dhar, P. Mahadani, S. Ghosh
{"title":"DNA Barcoding of Red Sea Fishes from Saudi Arabia – The first approach","authors":"Subrata Trivedi, Raafat Affan, A. Alessa, A. Ansari, Bishal Dhar, P. Mahadani, S. Ghosh","doi":"10.2478/DNA-2014-0003","DOIUrl":"https://doi.org/10.2478/DNA-2014-0003","url":null,"abstract":"","PeriodicalId":446240,"journal":{"name":"DNA Barcodes","volume":"388 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129172017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 27
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信