Biomedical Spectroscopy and Imaging最新文献_第8页

Building up spectral libraries for mapping erythrocytes by hyperspectral dark field microscopy 建立高光谱暗场显微镜测定红细胞的光谱文库

Biomedical Spectroscopy and Imaging Pub Date : 2016-01-01 DOI: 10.3233/BSI-160133

Marco Conti, R. Scanferlato, M. Louka, A. Sansone, Carla Marzetti, C. Ferreri

{"title":"Building up spectral libraries for mapping erythrocytes by hyperspectral dark field microscopy","authors":"Marco Conti, R. Scanferlato, M. Louka, A. Sansone, Carla Marzetti, C. Ferreri","doi":"10.3233/BSI-160133","DOIUrl":"https://doi.org/10.3233/BSI-160133","url":null,"abstract":"BACKGROUND: Red blood cells (RBC) are obtained by non-invasive methods and widely used for diagnostic tests of health status. Hyperspectral Dark Field Microscopy (HDFM) is a promising technique for nanoscale bio imaging and spectral analysis without additional sample preparation. OBJECTIVE: Develop a protocol for human RBC characterization by HDFM, checking the feasibility of a reference spectral library that can image and afford a new comprehensive descriptor of RBC status. METHOD: A step-by-step protocol for HDFM measurement of human RBC was for the first time established using 5 µl of EDTA-treated whole blood from healthy adults (n = 30). Hyperspectral characteristics of solutions/suspensions at biological concentrations of phospholipids, hemoglobin, spectrin, cholesterol and protoporphyrin IX, as the most relevant RBC components, were also determined. RESULTS: A library made of 8 end-member spectra and classification of their spectral distribution carried out by Single Angle Mapper (SAM) were determined, furnishing a comprehensive mapping and descriptor of healthy human RBC. The spectra of single components allowed some of the RBC spectral bands to be attributed. CONCLUSIONS: This work reports for the first time the hyperspectral optical imaging of the human RBC by a library made of 8 scattering spectra, whose spectral signatures are compared with those of the main RBC molecular components. The percent distribution of the spectral end-members was also achieved, thus giving for the first time the HDFM mapping of human healthy RBCs. The protocol developed herein allows the clinical potential of hyperspectral imaging to be developed for the use of RBC mapping in health and disease.","PeriodicalId":44239,"journal":{"name":"Biomedical Spectroscopy and Imaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/BSI-160133","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69856242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

The effect of irradiance and integration time in in vivo normal skin Raman measurements assessed by multivariate statistical analysis 通过多元统计分析评估辐照度和积分时间对体内正常皮肤拉曼测量的影响

Biomedical Spectroscopy and Imaging Pub Date : 2016-01-01 DOI: 10.3233/BSI-160140

A. Elka, Violetta Moulia, P. Spyridonos, N. Kourkoumelis

{"title":"The effect of irradiance and integration time in in vivo normal skin Raman measurements assessed by multivariate statistical analysis","authors":"A. Elka, Violetta Moulia, P. Spyridonos, N. Kourkoumelis","doi":"10.3233/BSI-160140","DOIUrl":"https://doi.org/10.3233/BSI-160140","url":null,"abstract":"BACKGROUND: The successful discrimination of the subtle spectral characteristics of human skin in Raman spectra requires optimal acquisition parameters. We explore the translational momentum of Raman spectroscopy towards clinical practice by fine-tuning two basic experimental parameters (irradiance and integration time) of a portable Raman system used in skin measurements. OBJECTIVE: The aim of this study is to construct a generic protocol for recording the optimal Raman signal for in vivo skin measurements. METHODS: In vivo spectra were collected from two individuals of normal Fitzpatrick type III skin type. We assessed two different irradiation setups with three different integration times each by separating the raw signal from the noise using multivariate analysis. RESULTS: Our results showed that under a time threshold no optimal measurement conditions can be achieved. On the other hand, increased laser power and acquisition time do not offer a significant advantage over the selected lower values. Baseline correction is the most critical component for analysing normalized skin Raman spectra. CONCLUSIONS: A simple working protocol based on multivariate statistics offers the relative adjustment of irradiance and signal integration time among other experimental parameters that must be examined for optimal Raman measurements of skin.","PeriodicalId":44239,"journal":{"name":"Biomedical Spectroscopy and Imaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/BSI-160140","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69856496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A dual-modal optical system combining depth-sensitive laser induced fluorescence (LIF) spectroscopy and optical coherence tomography (OCT) for analyzing layered biological tissue 一种结合深度敏感激光诱导荧光(LIF)光谱和光学相干断层扫描(OCT)的双峰光学系统，用于分析层状生物组织

Biomedical Spectroscopy and Imaging Pub Date : 2016-01-01 DOI: 10.3233/BSI-160147

K. M. Khan, Ragesh Kumar, Hemant Krishna, K. D. Rao, S. Majumder

引用次数: 4

Design and first applications of a flexible Raman micro-spectroscopic system for biological imaging 生物成像柔性拉曼微光谱系统的设计和首次应用

Biomedical Spectroscopy and Imaging Pub Date : 2016-01-01 DOI: 10.3233/BSI-160141

R. Kiselev, I. Schie, S. Aškrabić, C. Krafft, J. Popp

引用次数: 23

Cholesterol: An evergreen molecule in biology 胆固醇:生物学中的常绿分子

Biomedical Spectroscopy and Imaging Pub Date : 2016-01-01 DOI: 10.3233/BSI-160159

G. Kumar, A. Chattopadhyay

引用次数: 15

The early development and application of FTIR difference spectroscopy to membrane proteins: A personal perspective FTIR差分光谱在膜蛋白上的早期发展和应用:个人观点

Biomedical Spectroscopy and Imaging Pub Date : 2016-01-01 DOI: 10.3233/BSI-160148

K. Rothschild

{"title":"The early development and application of FTIR difference spectroscopy to membrane proteins: A personal perspective","authors":"K. Rothschild","doi":"10.3233/BSI-160148","DOIUrl":"https://doi.org/10.3233/BSI-160148","url":null,"abstract":"Membrane proteins facilitate some of the most important cellular processes including energy conversion, ion trans- port and signal transduction. While conventional infrared absorption provides information about membrane protein secondary structure, a major challenge is to develop a dynamic picture of the functioning of membrane proteins at the molecular level. The introduction of FTIR difference spectroscopy around 1980 to study structural changes in membrane proteins along with a number of associated techniques including protein isotope labeling, site-directed mutagenesis, polarization dichroism, atten- uated total reflection and time-resolved spectroscopy have led to significant progress towards this goal. It is now possible to routinely detect conformational changes of individual amino acid residues, backbone peptides, binding ligands, chromophores and even internal water molecules under physiological conditions with time-resolution down to nanoseconds. The advent of ultrafast pulsed-IR lasers has pushed this time-resolution down to femtoseconds. The early development of FTIR difference spectroscopy as applied to membrane proteins with special focus on bacteriorhodopsin is reviewed from a personal perspective.","PeriodicalId":44239,"journal":{"name":"Biomedical Spectroscopy and Imaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/BSI-160148","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69856960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Analysis of protein microarrays by FTIR imaging 蛋白质微阵列的FTIR成像分析

Biomedical Spectroscopy and Imaging Pub Date : 2016-01-01 DOI: 10.3233/BSI-160137

J. D. Meutter, Kheiro-Mouna Derfoufi, E. Goormaghtigh

{"title":"Analysis of protein microarrays by FTIR imaging","authors":"J. D. Meutter, Kheiro-Mouna Derfoufi, E. Goormaghtigh","doi":"10.3233/BSI-160137","DOIUrl":"https://doi.org/10.3233/BSI-160137","url":null,"abstract":"BACKGROUND: Proteins are sensitive to environmental conditions. Whether they are produced for therapeutic purposes or for fundamental research, the integrity of their structure and post-traductional modifications are key issues. Measuring glycosylation or phosphorylation level as well as their secondary structure most often rely on complex and indirect experiments. Infrared spectroscopy presents a series of advantages related to its multivariate character. There is a lack of high-throughput methods able to analyse these parameters. OBJECTIVE: In this paper we attempted to combine protein microarrays and infrared imaging for high throughput analysis of proteins. METHODS: A protein microarrayer was used to produce protein microarrays on BaF2 slides transparent in the mid-infrared. Spot density was about 25 spots/mm 2 . A 128 × 128 focal plane array infrared detector was used to record images of the protein microarrays. RESULTS: We show that 100 µm diameter spot are easily analyzed. Spots obtained with low protein concentrations, resulting in an average of a single protein monolayer (ca 3 fg/µm 2 for a 66 kDa protein) provided good quality spectra. CONCLUSIONS: Infrared imaging is a label free, high throughput method, able to analyse protein microarrays and to take advantage from the wide information available in the infrared spectra.","PeriodicalId":44239,"journal":{"name":"Biomedical Spectroscopy and Imaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/BSI-160137","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69856826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Structural response of genomic DNA from grapevine (Vitis vinifera L.) varieties to microwaves irradiation: A Fourier transform infrared spectroscopy assessment 葡萄品种基因组DNA对微波辐射的结构响应:傅里叶变换红外光谱评估

Biomedical Spectroscopy and Imaging Pub Date : 2016-01-01 DOI: 10.3233/BSI-160138

C. Tripon, C. Muntean, E. Surducan, I. Bratu, A. Halmagyi, A. Coste

引用次数: 1

An infrared spectroscopic blood test for non-small cell lung carcinoma and subtyping into pulmonary squamous cell carcinoma or adenocarcinoma 非小细胞肺癌的红外光谱血液测试和肺鳞状细胞癌或腺癌亚型

Biomedical Spectroscopy and Imaging Pub Date : 2016-01-01 DOI: 10.3233/BSI-160144

J. Ollesch, D. Theegarten, M. Altmayer, K. Darwiche, T. Hager, G. Stamatis, K. Gerwert

{"title":"An infrared spectroscopic blood test for non-small cell lung carcinoma and subtyping into pulmonary squamous cell carcinoma or adenocarcinoma","authors":"J. Ollesch, D. Theegarten, M. Altmayer, K. Darwiche, T. Hager, G. Stamatis, K. Gerwert","doi":"10.3233/BSI-160144","DOIUrl":"https://doi.org/10.3233/BSI-160144","url":null,"abstract":"BACKGROUND: Lung cancer is the leading cause of death for male and female cancer patients alike. Early diagnosis improves prognosis. A blood test would be a valuable support. OBJECTIVE: Infrared spectroscopy provides a label-free biochemical fingerprint of a sample. A study was conducted on 161 patients with initial cancer suspicion to identify and verify spectral biomarker candidate patterns to detect non-small cell lung carcinoma (NSCLC). METHODS: Blood serum and plasma samples were analysed with an automated FTIR spectroscopic system. Two pattern recognition algorithms and two classifiers were applied. Monte Carlo cross validation was performed with linear discriminant analysis and random forest classification. RESULTS: Marker patterns for the discrimination of cancer from clinically relevant disease control patients were identified in FTIR spectra of blood samples. An accuracy of up to 79% was achieved. Squamous cell and adenocarcinoma patients were separable with an accuracy of 80%. CONCLUSIONS: The study demonstrates the applicability of FTIR spectroscopic blood testing for lung cancer detection. Evidence for cancer subtype discrimination is given. With an improved performance, the method could be developed as a routine diagnostic tool for blood testing detecting NSCLC.","PeriodicalId":44239,"journal":{"name":"Biomedical Spectroscopy and Imaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/BSI-160144","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69856592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Cholesterol and nicotinic acetylcholine receptor: An intimate nanometer-scale spatial relationship spanning the billion year time-scale 胆固醇和尼古丁乙酰胆碱受体:一个亲密的纳米尺度空间关系跨越十亿年的时间尺度

Biomedical Spectroscopy and Imaging Pub Date : 2016-01-01 DOI: 10.3233/BSI-160158

F. Barrantes

引用次数: 3