Biomedical Spectroscopy and Imaging最新文献

{"title":"The use of FTIR and Raman spectroscopy in combination with chemometrics for analysis of biomolecules in biomedical fluids: A review","authors":"A. Rohman, A. Windarsih, E. Lukitaningsih, M. Rafi, K. Betania, N. Fadzillah","doi":"10.3233/bsi-200189","DOIUrl":"https://doi.org/10.3233/bsi-200189","url":null,"abstract":"Fourier transform infrared (FTIR) and Raman spectroscopy are complementary techniques, typically called vibrational spectroscopy. Both techniques allow simple, rapid, non-destructive, specific, providing fingerprint spectra, and real-time \u0000analytical method for analysis of molecules in different states. Besides, these methods are simple without any excessive sample \u0000pre-treatment, therefore, they are sometimes called as “green analytical methods”. Biofluids have several biomolecules such as \u0000lipid, protein, nucleic acids, and carbohydrates. These biomolecules can be used as biomarkers to detect some types of diseases, \u0000since biomolecules are in direct contact with the human organs. FTIR and Raman spectra of biofluids are complex in nature, \u0000therefore sophisticated statistical techniques, known as chemometrics, must be used to solve the analytical problems related to \u0000quantitative analysis purposes. The objective of this review is to show the capability of FTIR and Raman spectroscopic techniques in combination with chemometrics techniques to analyze the biomolecules in biofluids through an extensive literature \u0000review. During performing this review, several databases in Science citation index, Scopus PubMed, and Google Scholar related \u0000to the topics are identified and downloaded. With the present review, it is known that FTIR and Raman techniques are rapid \u0000method for screening certain diseases by identifying the level changes of some biomolecules. In the future, this method will be \u0000widely used for clinicians as new diagnostic tools for many diseases.","PeriodicalId":44239,"journal":{"name":"Biomedical Spectroscopy and Imaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/bsi-200189","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41629619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review of applications of Raman spectroscopy in immunology","authors":"N. Chaudhary, Claire Wynne, A. Meade","doi":"10.3233/bsi-200198","DOIUrl":"https://doi.org/10.3233/bsi-200198","url":null,"abstract":"Vibrational spectroscopic techniques have recently gained increasing clinical importance as non-invasive, rapid and inexpensive methods to obtain information on the content of biological samples. For some time Raman spectroscopy has been involved in preclinical applications, mainly in the cancer space, with evolving applications towards new horizons in the dermatology and companion diagnostics arena. It is attractive as an analytical technique due to its exquisite sensitivity, labelfree operation and low water detectivity such that in-vivo applications are possible. In cytometry, Raman spectroscopy has been applied to the analysis of single cells providing a label-free alternative to cell classification approaches in the laboratory. In this review we collate in-vitro, ex-vivo and in-vivo examples of research using Raman spectroscopy for the detection, quantification and analysis of immune signaling at the cellular level. While cancer biology has recently focussed on the role of immunological signals in the development of the disease, it is timely to examine these applications as research in this space evolves.","PeriodicalId":44239,"journal":{"name":"Biomedical Spectroscopy and Imaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/bsi-200198","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69857085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Potential of Utilizing Mid-Energy X-Rays for In-Line Phase Sensitive Breast Cancer Imaging.","authors":"F H Omoumi,&nbsp;M U Ghani,&nbsp;M D Wong,&nbsp;Y Li,&nbsp;B Zheng,&nbsp;A Yan,&nbsp;P A Jenkins,&nbsp;X Wu,&nbsp;H Liu","doi":"10.3233/BSI-200204","DOIUrl":"https://doi.org/10.3233/BSI-200204","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this study is to demonstrate the potential of utilizing mid-energy x-rays for in-line phase-sensitive breast cancer imaging by phantom studies.</p><p><strong>Methods: </strong>The midenergy (50-80kV) in-line phase sensitive imaging prototype was used to acquire images of the contrast-detail mammography (CDMAM) phantom, an ACR accreditation phantom, and an acrylic edge phantom. The low-dose mid-energy phase-sensitive images were acquired at 60 kV with a radiation dose of 0.9 mGy, while the high-energy phase-sensitive images were acquired at 90 kV with a radiation dose of 1.2 mGy. The Phase-Attenuation Duality (PAD) principle for soft tissue was used for the phase retrieval. A blind observer study was conducted and paired-sample T-test were performed to compare the mean differences in the two imaging systems.</p><p><strong>Results: </strong>The correct detection ratio for the CDMAM phantom for phase-contrast images acquired by the low-dose mid-energy system was 56.91%, whereas images acquired by the high-energy system correctly revealed only 40.97% of discs. The correct detection ratios were 57.88% and 43.41% for phase-retrieved images acquired by the low-dose mid-energy and high-energy imaging systems, respectively. The reading scores for all three groups of objects in the ACR phantom were higher for the mid energy imaging system as compared to the high-energy system for both phase-contrast and phase- retrieved images. The calculated edge enhancement index (EEI) from the acrylic edge phantom image for the mid-energy system was higher than that calculated for the high-energy imaging system. The quantitative analyses showed a higher Contrast to Noise Ratio (CNR) as well as a higher Figure of Merit (FOM) in images acquired by the low-dose mid-energy imaging system.</p><p><strong>Conclusion: </strong>The PAD based retrieval method can be applied in mid-energy system without remarkably affecting the image quality, and in fact, it improves the lesion detectability with a patient dose saving of 25%.</p>","PeriodicalId":44239,"journal":{"name":"Biomedical Spectroscopy and Imaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/BSI-200204","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39242653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Label-free digital pathology by infrared imaging","authors":"F. Großerueschkamp, K. Gerwert","doi":"10.3233/bsi-200196","DOIUrl":"https://doi.org/10.3233/bsi-200196","url":null,"abstract":"","PeriodicalId":44239,"journal":{"name":"Biomedical Spectroscopy and Imaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/bsi-200196","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69857021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A gold nanoparticle conjugate with photosystem I and photosystem II for development of a biohybrid water-splitting photocatalyst","authors":"Kousuke Kawahara, Natsuko Inoue-Kahino, Keisuke Namie, Yuki Kato, T. Tomo, Y. Shibata, Y. Kashino, T. Noguchi","doi":"10.3233/bsi-200200","DOIUrl":"https://doi.org/10.3233/bsi-200200","url":null,"abstract":"","PeriodicalId":44239,"journal":{"name":"Biomedical Spectroscopy and Imaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/bsi-200200","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69857128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel application of confocal Raman microscopy to determine the microstructure of fermented dairy products including the spatial distribution of proteins, lipids and carbohydrates","authors":"Anita P. Pax, J. Sheehan","doi":"10.3233/bsi-200201","DOIUrl":"https://doi.org/10.3233/bsi-200201","url":null,"abstract":"","PeriodicalId":44239,"journal":{"name":"Biomedical Spectroscopy and Imaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/bsi-200201","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69857167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of optical properties of Ba0.2Sr0.8TiO3 thin films for a glucose sensor implementation","authors":"E. Palupi, H. Alatas, Irzaman, Y. Suryana, A. Aridarma, R. Umam, B. B. Andriana, H. Sato","doi":"10.3233/bsi-200197","DOIUrl":"https://doi.org/10.3233/bsi-200197","url":null,"abstract":"","PeriodicalId":44239,"journal":{"name":"Biomedical Spectroscopy and Imaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/bsi-200197","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69857072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determining T2 relaxation time and stroke onset relationship in ischaemic stroke within apparent diffusion coefficient-defined lesions. A user-independent method for quantifying the impact of stroke in the human brain.","authors":"Michael J. Knight, Robin A. Damion, Bryony L. McGarry, R. Bosnell, K. Jokivarsi, O. Gröhn, P. Jezzard, G. Harston, D. Carone, J. Kennedy, S. El-Tawil, Jennifer Elliot, K. Muir, P. Clatworthy, R. Kauppinen","doi":"10.3233/BSI-190185","DOIUrl":"https://doi.org/10.3233/BSI-190185","url":null,"abstract":"Background and Objective\u0000In hyperacute ischaemic stroke, T2 of cerebral water increases with time. Quantifying this change may be informative of the extent of tissue damage and onset time. Our objective was to develop a user-unbiased method to measure the effect of cerebral ischaemia on T2 to study stroke onset time-dependency in human acute stroke lesions.\u0000\u0000\u0000Methods\u0000Six rats were subjected to permanent middle cerebral occlusion to induce focal ischaemia, and a consecutive cohort of acute stroke patients (n = 38) were recruited within 9 hours from symptom onset. T1-weighted structural, T2 relaxometry, and diffusion MRI for apparent diffusion coefficient (ADC) were acquired. Ischaemic lesions were defined as regions of lowered ADC. The median T2 difference (ΔT2) between lesion and contralateral non-ischaemic control region was determined by the newly-developed spherical reference method, and data compared to that obtained by the mirror reference method. Linear regressions and receiver operating characteristics (ROC) were compared between the two methods.\u0000\u0000\u0000Results\u0000ΔT2 increases linearly in rat brain ischaemia by 1.9 ± 0.8 ms/h during the first 6 hours, as determined by the spherical reference method. In patients, ΔT2 linearly increases by 1.6 ± 1.4 and 1.9 ± 0.9 ms/h in the lesion, as determined by the mirror reference and spherical reference method, respectively. ROC analyses produced areas under the curve of 0.83 and 0.71 for the spherical and mirror reference methods, respectively.\u0000\u0000\u0000Conclusions\u0000Data from the spherical reference method showed that the median T2 increase in the ischaemic lesion is correlated with stroke onset time in a rat as well as in a human patient cohort, opening the possibility of using the approach as a timing tool in clinics.","PeriodicalId":44239,"journal":{"name":"Biomedical Spectroscopy and Imaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/BSI-190185","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41539078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of a single distance phase retrieval algorithm on spatial resolution in X-ray inline phase sensitive imaging.","authors":"Muhammad U. Ghani, Bradley Gregory, Farid H Omoumi, B. Zheng, A. Yan, Xizeng Wu, Hong Liu","doi":"10.3233/BSI-190186","DOIUrl":"https://doi.org/10.3233/BSI-190186","url":null,"abstract":"A single-projection based phase retrieval method based on the phase attenuation duality principle (PAD) was used to compare the spatial resolution of the acquired phase sensitive and PAD processed phase retrieved images. An inline phase sensitive prototype was used to acquire the phase sensitive images. The prototype incorporates a micro-focus x-ray source and a flat panel detector with a 50 μm pixel pitch. A phantom composed of a 2 cm thick 50-50 adipose-glandular mimicking slab sandwiched with a 0.82 cm thick slanted PMMA sharp edge was used. Phase sensitive image of the phantom was acquired at 120 kV, 3.35 mAs with a 16 μm tube focal spot size under a geometric magnification (M) of 2.5. The PAD based method was applied to the acquired phase sensitive image for the retrieval of phase values. With necessary data processing, modulation transfer function (MTF) curves were determined for the estimation and comparison of the spatial resolution. The PAD processed phase retrieved values of the phantom were in good agreement with the theoretically calculated values. Phase sensitive images showed higher spatial resolution at all spatial frequencies compared to the phase retrieved images. It was noted that the high-frequency signal components in the retrieved image were suppressed that resulted in lower MTF values. When compared to the phase sensitive image, the cutoff resolution (10% MTF) for phase retrieved image dropped 32% from 15.6 lp/mm (32μm) to 10.6 lp/mm (47μm). The resolution offered by this phase sensitive prototype is radiographically enough to detect breast cancer.","PeriodicalId":44239,"journal":{"name":"Biomedical Spectroscopy and Imaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/BSI-190186","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44792735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of polymer and gold functionalization on the magnetic properties of magnetite nanoparticles","authors":"Staci Adams, Sara Bonabi, A’Lester C. Allen, G. Roseman, A. P. Ramirez, G. Millhauser, Jin Z. Zhang","doi":"10.3233/BSI-180182","DOIUrl":"https://doi.org/10.3233/BSI-180182","url":null,"abstract":"","PeriodicalId":44239,"journal":{"name":"Biomedical Spectroscopy and Imaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/BSI-180182","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46747095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}