A review of applications of Raman spectroscopy in immunology

IF 0.3 Q4 SPECTROSCOPY

Biomedical Spectroscopy and Imaging Pub Date : 2020-01-01 DOI:10.3233/bsi-200198

N. Chaudhary, Claire Wynne, A. Meade

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引用次数: 4

Abstract

Vibrational spectroscopic techniques have recently gained increasing clinical importance as non-invasive, rapid and inexpensive methods to obtain information on the content of biological samples. For some time Raman spectroscopy has been involved in preclinical applications, mainly in the cancer space, with evolving applications towards new horizons in the dermatology and companion diagnostics arena. It is attractive as an analytical technique due to its exquisite sensitivity, labelfree operation and low water detectivity such that in-vivo applications are possible. In cytometry, Raman spectroscopy has been applied to the analysis of single cells providing a label-free alternative to cell classification approaches in the laboratory. In this review we collate in-vitro, ex-vivo and in-vivo examples of research using Raman spectroscopy for the detection, quantification and analysis of immune signaling at the cellular level. While cancer biology has recently focussed on the role of immunological signals in the development of the disease, it is timely to examine these applications as research in this space evolves.

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拉曼光谱在免疫学中的应用综述

近年来，振动光谱技术作为一种无创、快速、廉价的获取生物样品含量信息的方法，在临床中具有越来越重要的意义。一段时间以来，拉曼光谱已经参与临床前应用，主要是在癌症领域，随着皮肤病学和伴随诊断领域的应用向新的领域发展。它是一种有吸引力的分析技术，由于其精致的灵敏度，无标签操作和低水检测，使体内应用成为可能。在细胞术中，拉曼光谱已被应用于单个细胞的分析，为实验室中的细胞分类方法提供了一种无标记的替代方法。在这篇综述中，我们整理了利用拉曼光谱在细胞水平上检测、定量和分析免疫信号的体外、离体和体内研究实例。虽然癌症生物学最近关注免疫信号在疾病发展中的作用，但随着这一领域研究的发展，检查这些应用是及时的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Biomedical Spectroscopy and Imaging SPECTROSCOPY-

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期刊介绍： Biomedical Spectroscopy and Imaging (BSI) is a multidisciplinary journal devoted to the timely publication of basic and applied research that uses spectroscopic and imaging techniques in different areas of life science including biology, biochemistry, biotechnology, bionanotechnology, environmental science, food science, pharmaceutical science, physiology and medicine. Scientists are encouraged to submit their work for publication in the form of original articles, brief communications, rapid communications, reviews and mini-reviews. Techniques covered include, but are not limited, to the following: • Vibrational Spectroscopy (Infrared, Raman, Teraherz) • Circular Dichroism Spectroscopy • Magnetic Resonance Spectroscopy (NMR, ESR) • UV-vis Spectroscopy • Mössbauer Spectroscopy • X-ray Spectroscopy (Absorption, Emission, Photoelectron, Fluorescence) • Neutron Spectroscopy • Mass Spectroscopy • Fluorescence Spectroscopy • X-ray and Neutron Scattering • Differential Scanning Calorimetry • Atomic Force Microscopy • Surface Plasmon Resonance • Magnetic Resonance Imaging • X-ray Imaging • Electron Imaging • Neutron Imaging • Raman Imaging • Infrared Imaging • Terahertz Imaging • Fluorescence Imaging • Near-infrared spectroscopy.