Journal of Physics-Photonics最新文献

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Smartphone-based diagnostics with coherent and infrared imaging for cultural heritage 基于智能手机的文化遗产相干和红外成像诊断技术
IF 3.8
Journal of Physics-Photonics Pub Date : 2024-08-14 DOI: 10.1088/2515-7647/ad6abc
Claudia Daffara, Dario Ambrosini
{"title":"Smartphone-based diagnostics with coherent and infrared imaging for cultural heritage","authors":"Claudia Daffara, Dario Ambrosini","doi":"10.1088/2515-7647/ad6abc","DOIUrl":"https://doi.org/10.1088/2515-7647/ad6abc","url":null,"abstract":"Nondestructive optical techniques are crucial in heritage science for monitoring the condition of artworks in full field. Various imaging methods based on infrared and interferometry techniques have been proposed, but they often require specialized training and expensive equipment. This paper explores the emerging field of smartphone science and its potential to revolutionize artwork diagnostics, especially for cultural institutions with limited budgets. The smartphone science approach is divided into using the device ‘as is’ or enhancing it with add-on sensors. After a concise overview of smartphone sensing in different fields, the paper demonstrates smartphone-based optical diagnostics on traditional wooden painting models, employing coherent techniques like laser speckle imaging and moiré fringe technique, and infrared techniques like reflectography and thermography. The comparison of obtained results with established instrumentation in the field clearly shows that smartphone-based diagnostics have the potential to greatly contribute to cultural heritage preservation and conservation, transforming the field’s accessibility and cost-effectiveness.","PeriodicalId":44008,"journal":{"name":"Journal of Physics-Photonics","volume":"3 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142222046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Topological THz on-chip valley–spin converter 拓扑太赫兹片上山谷-自旋转换器
IF 3.8
Journal of Physics-Photonics Pub Date : 2024-08-13 DOI: 10.1088/2515-7647/ad68de
Yudong Ren, Xinrui Li, Ning Han, Li Zhang, Rui Zhao, Qiaolu Chen, Yuze Hu, Mingyu Tong, Song Han, Yihao Yang
{"title":"Topological THz on-chip valley–spin converter","authors":"Yudong Ren, Xinrui Li, Ning Han, Li Zhang, Rui Zhao, Qiaolu Chen, Yuze Hu, Mingyu Tong, Song Han, Yihao Yang","doi":"10.1088/2515-7647/ad68de","DOIUrl":"https://doi.org/10.1088/2515-7647/ad68de","url":null,"abstract":"Topological photonics offers a powerful platform for next-generation nanophotonic chips, capitalizing on their remarkable resilience to disorder and defects. Among the two-dimensional (2D) photonic topological insulators, valley-Hall (VH) and pseudo-spin-Hall (PSH) topological insulators have emerged as the most practical designs, as they do not require breaking time-reversal symmetry. These photonic topological insulators support robust edge states, demonstrating promising potential for a wide range of applications, from on-chip communication to optical computing and sensing. However, the conversion between distinct topological phases (VH and PSH) in terahertz (THz) band has not been achieved. Here we experimentally demonstrate a THz on-chip spin–valley converter through adiabatic evolution in 2D parameter space without closing the bulk bandgap. By leveraging the adiabatic phase transition, we confirm the high-efficiency conversion between two valley states in a valley–spin–valley converter. In addition, we verify the robustness of THz PSH topological energy transport through sharply twisted corners. Our findings not only advance the understanding of topological phases in photonics but also hold promise for the development of innovative photonic devices with enhanced performance and functionality.","PeriodicalId":44008,"journal":{"name":"Journal of Physics-Photonics","volume":"6 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142222047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Multi-modal flexible and inexpensive plasmonic metasurface for wide range of refractive index sensing 用于大范围折射率传感的多模式灵活廉价质子元表面
IF 3.8
Journal of Physics-Photonics Pub Date : 2024-08-07 DOI: 10.1088/2515-7647/ad6963
Vaswati Biswas and R Vijaya
{"title":"Multi-modal flexible and inexpensive plasmonic metasurface for wide range of refractive index sensing","authors":"Vaswati Biswas and R Vijaya","doi":"10.1088/2515-7647/ad6963","DOIUrl":"https://doi.org/10.1088/2515-7647/ad6963","url":null,"abstract":"A plasmonic metasurface containing nanobumps of sub-wavelength feature size arranged in a hexagonal pattern on a flexible substrate and covered with a thin film of gold is investigated as a refractive index (RI) sensor. The chosen polymer patterns coated with gold aid in activating the surface plasmon polariton modes. Using numerical calculations, it is shown that this surface can exhibit plasmonic effect with extremely shallow pattern height of 92.5 nm and minimal thickness of 25 nm of gold over it. The excitation of the plasmonic modes is confirmed using electric field profiles calculated at the relevant wavelengths. As the surface is highly sensitive to changes in the cladding index, and the chosen design aids in exciting three plasmon modes that are suitably well-separated in wavelength, this surface can be used for an extremely wide range of RI sensing because each mode contributes uniquely to a different range of RI. The results establish that the metasurface is suitable for a variety of applications, including gas detection with a sensitivity of 633 nm RIU−1 using mode-1, identifying SARS-CoV-2 viral molecules with a sensitivity of 428 nm RIU−1 using mode-2 and 238 nm RIU−1 using mode-3, and discriminating between normal and diseased brain tissues in the cerebrospinal fluid in the high-index range using mode-3. The prototype metasurface is made using a cost-effective soft lithography technique using an economical master mould. The inexpensive technique of fabrication, use of very thin metal film, and wavelength of detection lying within the visible to near infrared range imply a low-cost sensor. The structural and optical characterization of the prototype validates the numerical study of the sample.","PeriodicalId":44008,"journal":{"name":"Journal of Physics-Photonics","volume":"2 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141937207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Image scanning microscopy reconstruction by autocorrelation inversion 通过自相关反演重建图像扫描显微镜
IF 3.8
Journal of Physics-Photonics Pub Date : 2024-08-06 DOI: 10.1088/2515-7647/ad68dd
Daniele Ancora, Alessandro Zunino, Giuseppe Vicidomini and Alvaro H Crevenna
{"title":"Image scanning microscopy reconstruction by autocorrelation inversion","authors":"Daniele Ancora, Alessandro Zunino, Giuseppe Vicidomini and Alvaro H Crevenna","doi":"10.1088/2515-7647/ad68dd","DOIUrl":"https://doi.org/10.1088/2515-7647/ad68dd","url":null,"abstract":"Confocal laser scanning microscopy (CLSM) stands out as one of the most widely used microscopy techniques thanks to its three-dimensional imaging capability and its sub-diffraction spatial resolution, achieved through the closure of a pinhole in front of a single-element detector. However, the pinhole also rejects useful photons, and beating the diffraction limit comes at the price of irremediably compromising the signal-to-noise ratio (SNR) of the data. Image scanning microscopy (ISM) emerged as the rational evolution of CLSM, exploiting a small array detector in place of the pinhole and the single-element detector. Each sensitive element is small enough to achieve sub-diffraction resolution through the confocal effect, but the size of the whole detector is large enough to guarantee excellent collection efficiency and SNR. However, the raw data produced by an ISM setup consists of a 4D dataset, which can be seen as a set of confocal-like images. Thus, fusing the dataset into a single super-resolved image requires a dedicated reconstruction algorithm. Conventional methods are multi-image deconvolution, which requires prior knowledge of the system point spread functions (PSFs), or adaptive pixel reassignment (APR), which is effective only on a limited range of experimental conditions. In this work, we describe and validate a novel concept for ISM image reconstruction based on autocorrelation inversion. We leverage unique properties of the autocorrelation to discard low-frequency components and maximize the resolution of the reconstructed image without any assumption on the image or any knowledge of the PSF. Our results push the quality of the ISM reconstruction beyond the level provided by APR and open new perspectives for multi-dimensional image processing.","PeriodicalId":44008,"journal":{"name":"Journal of Physics-Photonics","volume":"78 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141937389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Three-wave mixing experiments in indium–tin–oxide thin-films with no phase matching 无相位匹配铟锡氧化物薄膜中的三波混合实验
IF 3.8
Journal of Physics-Photonics Pub Date : 2024-08-04 DOI: 10.1088/2515-7647/ad6799
Kyle Wynne, Marjan Bazian and Mark C Harrison
{"title":"Three-wave mixing experiments in indium–tin–oxide thin-films with no phase matching","authors":"Kyle Wynne, Marjan Bazian and Mark C Harrison","doi":"10.1088/2515-7647/ad6799","DOIUrl":"https://doi.org/10.1088/2515-7647/ad6799","url":null,"abstract":"One challenge of using nonlinear optical phenomena for practical applications is the need to perform phase-matching. Recently, epsilon-near-zero materials have been shown to demonstrate strong optical nonlinearities, in addition to their other unique properties. As suggested by their name, the permittivity of the material is close to zero for a certain wavelength range. We demonstrate that this small permittivity allows for efficient three-wave mixing interactions to take place in indium–tin–oxide thin films without the need for phase matching the pump and signal beams. The efficiency of the second-order nonlinear interactions is characterized, and cascaded three-wave mixing is demonstrated.","PeriodicalId":44008,"journal":{"name":"Journal of Physics-Photonics","volume":"34 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141937380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Monitoring cis-to-trans isomerization of azobenzene using Brillouin microscopy 利用布里渊显微镜监测偶氮苯的顺反异构过程
IF 3.8
Journal of Physics-Photonics Pub Date : 2024-07-16 DOI: 10.1088/2515-7647/ad5bd1
Zhe Wang, Qiyang Jiang, Chantal Barwig, Ankit Mishra, Krishna Ramesh and Christine Selhuber-Unkel
{"title":"Monitoring cis-to-trans isomerization of azobenzene using Brillouin microscopy","authors":"Zhe Wang, Qiyang Jiang, Chantal Barwig, Ankit Mishra, Krishna Ramesh and Christine Selhuber-Unkel","doi":"10.1088/2515-7647/ad5bd1","DOIUrl":"https://doi.org/10.1088/2515-7647/ad5bd1","url":null,"abstract":"Brillouin spectroscopy is commonly used to study the acoustic properties of materials. Here we explored its feasibility in studying the photoinduced isomerization of azobenzene. The isomerization of azobenzene changes the solution elastic modulus, and Brillouin scattering is sensitive to these changes. In this study, we experimentally demonstrated the photoswitching of azobenzene in DMSO using our home-made virtually imaged phased array-based high-resolution optical Brillouin spectrometer, and confirmed the results by ultraviolet–visible spectrophotometry. Remarkable Brillouin frequency shift variations were quantitatively recorded upon irradiation, and it was found that this method can indeed be used to monitor the isomerization process in situ. Importantly, our strategy also allows us to provide the relationship between the fraction of trans- and cis- azobenzene and the Brillouin frequency shift. This shows that Brillouin spectroscopy has broad prospects for the characterization of azobenzene isomerization and other photoresponsive materials.","PeriodicalId":44008,"journal":{"name":"Journal of Physics-Photonics","volume":"35 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141722187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Roadmap on perovskite light-emitting diodes 过氧化物发光二极管路线图
IF 3.8
Journal of Physics-Photonics Pub Date : 2024-07-10 DOI: 10.1088/2515-7647/ad46a6
Ziming Chen, Robert L Z Hoye, Hin-Lap Yip, Nadesh Fiuza-Maneiro, Iago López-Fernández, Clara Otero-Martínez, Lakshminarayana Polavarapu, Navendu Mondal, Alessandro Mirabelli, Miguel Anaya, Samuel D Stranks, Hui Liu, Guangyi Shi, Zhengguo Xiao, Nakyung Kim, Yunna Kim, Byungha Shin, Jinquan Shi, Mengxia Liu, Qianpeng Zhang, Zhiyong Fan, James C Loy, Lianfeng Zhao, Barry P Rand, Habibul Arfin, Sajid Saikia, Angshuman Nag, Chen Zou, Lih Y Lin, Hengyang Xiang, Haibo Zeng, Denghui Liu, Shi-Jian Su, Chenhui Wang, Haizheng Zhong, Tong-Tong Xuan, Rong-Jun Xie, Chunxiong Bao, Feng Gao, Xiang Gao, Chuanjiang Qin, Young-Hoon Kim and Matthew C Beard
{"title":"Roadmap on perovskite light-emitting diodes","authors":"Ziming Chen, Robert L Z Hoye, Hin-Lap Yip, Nadesh Fiuza-Maneiro, Iago López-Fernández, Clara Otero-Martínez, Lakshminarayana Polavarapu, Navendu Mondal, Alessandro Mirabelli, Miguel Anaya, Samuel D Stranks, Hui Liu, Guangyi Shi, Zhengguo Xiao, Nakyung Kim, Yunna Kim, Byungha Shin, Jinquan Shi, Mengxia Liu, Qianpeng Zhang, Zhiyong Fan, James C Loy, Lianfeng Zhao, Barry P Rand, Habibul Arfin, Sajid Saikia, Angshuman Nag, Chen Zou, Lih Y Lin, Hengyang Xiang, Haibo Zeng, Denghui Liu, Shi-Jian Su, Chenhui Wang, Haizheng Zhong, Tong-Tong Xuan, Rong-Jun Xie, Chunxiong Bao, Feng Gao, Xiang Gao, Chuanjiang Qin, Young-Hoon Kim and Matthew C Beard","doi":"10.1088/2515-7647/ad46a6","DOIUrl":"https://doi.org/10.1088/2515-7647/ad46a6","url":null,"abstract":"In recent years, the field of metal-halide perovskite emitters has rapidly emerged as a new community in solid-state lighting. Their exceptional optoelectronic properties have contributed to the rapid rise in external quantum efficiencies (EQEs) in perovskite light-emitting diodes (PeLEDs) from <1% (in 2014) to over 30% (in 2023) across a wide range of wavelengths. However, several challenges still hinder their commercialization, including the relatively low EQEs of blue/white devices, limited EQEs in large-area devices, poor device stability, as well as the toxicity of the easily accessible lead components and the solvents used in the synthesis and processing of PeLEDs. This roadmap addresses the current and future challenges in PeLEDs across fundamental and applied research areas, by sharing the community’s perspectives. This work will provide the field with practical guidelines to advance PeLED development and facilitate more rapid commercialization.","PeriodicalId":44008,"journal":{"name":"Journal of Physics-Photonics","volume":"23 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141586130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Portable fiber-based double nanohole optical tweezer for trapping small proteins 用于捕获小蛋白质的便携式光纤双纳米孔光学镊子
IF 3.8
Journal of Physics-Photonics Pub Date : 2024-07-09 DOI: 10.1088/2515-7647/ad5bd0
Veerpal Kaur, Demelza Wright, Samuel Mathew, Matthew Peters, Maria Zacharopoulou, Shang Hua Yang, Laura S Itzhaki, Ivet Bahar, Reuven Gordon
{"title":"Portable fiber-based double nanohole optical tweezer for trapping small proteins","authors":"Veerpal Kaur, Demelza Wright, Samuel Mathew, Matthew Peters, Maria Zacharopoulou, Shang Hua Yang, Laura S Itzhaki, Ivet Bahar, Reuven Gordon","doi":"10.1088/2515-7647/ad5bd0","DOIUrl":"https://doi.org/10.1088/2515-7647/ad5bd0","url":null,"abstract":"We demonstrate the trapping and analysis of individual proteins using a portable optical fiber tweezer setup with a double-nanohole in a gold film coating the fiber’s end and aligned with the fiber core. The instrument was used to trap and analyze cytochrome c, carbonic anhydrase, bovine serum albumin, and PR65 (wild-type and various point mutants). This approach was compared with a free-space optical tweezer setup that requires alignment of the laser focus to the aperture, whereas the fiber-based approach is both portable and alignment-free, which holds promise for applications in antibody discovery, small molecule drug discovery, protein interaction analysis and other applications using the standard well-plate format.","PeriodicalId":44008,"journal":{"name":"Journal of Physics-Photonics","volume":"29 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141571562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Packaging of micro-lens arrays to photonic integrated circuits using beam shape evaluation 利用光束形状评估将微型透镜阵列封装到光子集成电路中
IF 3.8
Journal of Physics-Photonics Pub Date : 2024-07-02 DOI: 10.1088/2515-7647/ad5bd2
Kamil Gradkowski, Padraic E Morrissey and Peter O’Brien
{"title":"Packaging of micro-lens arrays to photonic integrated circuits using beam shape evaluation","authors":"Kamil Gradkowski, Padraic E Morrissey and Peter O’Brien","doi":"10.1088/2515-7647/ad5bd2","DOIUrl":"https://doi.org/10.1088/2515-7647/ad5bd2","url":null,"abstract":"We propose a method for aligning and attaching micro-lens arrays to photonic integrated circuits (PICs). Unlike the conventional approach of assessing power coupled to a fiber directly, our method utilizes a beam profiler. This profiler allows us to optimize the lens position by analyzing the transmitted beam shape from the PIC edge coupler through the lens. In conjunction, we employ grating couplers to introduce external light, acting as a ‘beacon’ for optimization. The use of grating couplers enables efficient coupling of external light into the PIC, providing a reference point for alignment. Importantly, our method accommodates both regular waveguide-side-up and upside-down (through-Silicon) orientations of the PIC. This versatility allows us to reproduce coupling results across a 6-channel array, demonstrating robust performance. This innovative approach not only ensures precise alignment and attachment but also opens up new possibilities for photonic packaging. The flexibility to work in different orientations is likely to lead to advancements in the design and assembly of photonic devices.","PeriodicalId":44008,"journal":{"name":"Journal of Physics-Photonics","volume":"138 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141516849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Beyond comparison: Brillouin microscopy and AFM-based indentation reveal divergent insights into the mechanical profile of the murine retina 超越比较:布里渊显微镜和基于原子力显微镜的压痕法揭示了小鼠视网膜机械特征的不同见解
IF 3.8
Journal of Physics-Photonics Pub Date : 2024-07-01 DOI: 10.1088/2515-7647/ad5ae3
Marcus Gutmann, Jana Bachir Salvador, Paul Müller, Kyoohyun Kim, Martin Schicht, Serhii Aif, Friedrich Paulsen, Lorenz Meinel, Jochen Guck and Stephanie Möllmert
{"title":"Beyond comparison: Brillouin microscopy and AFM-based indentation reveal divergent insights into the mechanical profile of the murine retina","authors":"Marcus Gutmann, Jana Bachir Salvador, Paul Müller, Kyoohyun Kim, Martin Schicht, Serhii Aif, Friedrich Paulsen, Lorenz Meinel, Jochen Guck and Stephanie Möllmert","doi":"10.1088/2515-7647/ad5ae3","DOIUrl":"https://doi.org/10.1088/2515-7647/ad5ae3","url":null,"abstract":"Mechanical tissue properties increasingly serve as pivotal phenotypic characteristics that are subject to change during development or pathological progression. The quantification of such material properties often relies on physical contact between a load-applying probe and an exposed sample surface. For most tissues, meeting these requirements entails an invasive preparation, which poses the risk of yielding mechanical properties that do not portray the physiological state of a tissue within a functioning organism. Brillouin microscopy has emerged as a non-invasive, optical technique that enables the assessment of mechanical cell and tissue properties with high spatio-temporal resolution. In optically transparent specimens, it does not require animal sacrifice, tissue dissection or sectioning. However, the extent to which results obtained from Brillouin microscopy allow to infer conclusions about potential results obtained with a contact-based technique, and vice versa, is unclear. Sources for discrepancies include the varying characteristic temporal and spatial scales, the directionality of measurement, environmental factors, and mechanical moduli probed. In this work, we addressed those aspects by quantifying the mechanical properties of acutely dissected murine retinae using Brillouin microscopy and atomic force microscopy (AFM)-based indentation measurements. Our results show a distinct mechanical profile of the retinal layers with respect to the Brillouin frequency shift, the Brillouin linewidth and the apparent Young’s modulus. Contrary to previous reports, our findings do not support a simple correlative relationship between Brillouin frequency shift and apparent Young’s modulus. Additionally, the divergent sensitivities of Brillouin microscopy and AFM-indentation measurements to structural features, as visualized by transmission electron microscopy, to cross-linking or changes post mortem underscore the dangers of assuming interchangeability between the two methods. In conclusion, our study advocates for viewing Brillouin microscopy and AFM-based indentation measurements as complementary tools, discouraging direct comparisons a priori and suggesting their combined use for a more comprehensive understanding of tissue mechanical properties.","PeriodicalId":44008,"journal":{"name":"Journal of Physics-Photonics","volume":"36 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141531176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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