Journal of the Belarusian State University. Biology最新文献_第2页

Lignin phenols as a basis for biopreparations 木质素酚作为生物修复的基础

Journal of the Belarusian State University. Biology Pub Date : 2021-07-05 DOI: 10.33581/2521-1722-2021-2-29-42

I. Kovalev, N. Kovaleva

引用次数: 0

Analysis of the efficiency factors of electrotransformation of Bacillus subtilis to inactivate the aroK gene by the method of homologous recombination 用同源重组法电转化枯草芽孢杆菌灭活aroK基因的效率因素分析

Journal of the Belarusian State University. Biology Pub Date : 2021-07-05 DOI: 10.33581/2521-1722-2021-2-64-73

Yui Chao, Aleksei V. Lahodzich

{"title":"Analysis of the efficiency factors of electrotransformation of Bacillus subtilis to inactivate the aroK gene by the method of homologous recombination","authors":"Yui Chao, Aleksei V. Lahodzich","doi":"10.33581/2521-1722-2021-2-64-73","DOIUrl":"https://doi.org/10.33581/2521-1722-2021-2-64-73","url":null,"abstract":"A hyper-osmotic electrotransformation method was developed for strain Bacillus subtilis. Sorbitol and mannitol are included in the hyper-osmotic electroporation medium and recovery medium. In this study, the hyper-osmotic electroporation method was optimised to increase the transformation efficiency of B. subtilis strain 5434 (non-transformable by chemical methods) by 430 fold, with a maximum value of 8.6 ⋅ 105 CFU/µg of integrative plasmid DNA. With the electroporation setted 25 µF, 23 kV/cm, 200 Ω, the method was optimised as follows: a) the OD600 value of the bacterial culture solution was increased to about 1.2, which significantly enhanced survival of bacteria and quantity of viable B.subtilis strain 5434 cells after electroporation; b) the elution frequency of washing solution (hyper-osmotic electroporation medium) for complement cells was increased from 3 to 5 times, resulted in significantly reducing the conductivity of the hyper-osmotic electoporation medium with competent cells (electrocompetent cultue), and effectively extending the pulse time under the same electric field strength; c) quantity of integrative plasmid DNA added to hyper-osmotic electrocompetent culture was optimised. These results indicate that increasing the number of viable B. subtilis strain 5434 cells and reducing the number of metal ions in the electroporation solution mix (integrative plasmid DNA, competent cells of B. subtilis strain 5434, electroporation medium) are useful approach to improve transfomation efficiency of B. subtilis strain 5434. Concentration of shikimic acid in the fermentation medium was quantified by high performance liquid chromatography. Quantification of shikimic acid revealed that B. subtilis strain 5434p4SA produced 403.98 ± 9.1 µg/mL of shikimic acid.","PeriodicalId":437681,"journal":{"name":"Journal of the Belarusian State University. Biology","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115048779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increasing the antimicrobial activity of Pseudomonas brassicacearum S-1 by inactivation of lysR3 and mtfA genes 通过灭活lysR3和mtfA基因，提高油菜假单胞菌S-1的抑菌活性

Journal of the Belarusian State University. Biology Pub Date : 2021-07-01 DOI: 10.33581/2521-1722-2021-2-52-63

Anna A. Muratova, M. Mandryk-Litvinkovich, L. Valentovich

引用次数: 0

The investigation of adaptogenic and hepatoprotective properties of Ilex paraguariensis on experimental models in vivo 巴拉圭冬青在动物模型上的适应性和保肝性研究

Journal of the Belarusian State University. Biology Pub Date : 2021-07-01 DOI: 10.33581/2521-1722-2021-2-43-51

A. I. Hubich, Svyatlana S. Okarakava, Darya V. Kopyleva, N. Jaafar, Dziyana V. Duts, Maryia I. Koval

引用次数: 0

Microsatellite markers in the study of polymorphism of domestic pig breeds (Sus scrofa domesticus) 微卫星标记在家猪品种多态性研究中的应用

Journal of the Belarusian State University. Biology Pub Date : 2021-06-28 DOI: 10.33581/2521-1722-2021-2-74-83

Alina A. Rabtsava, S. A. Kotava, Alexandra Ya. Hrebianchuk, A. I. Gandzha, Natallia V. Zhuryna, I. Tsybovsky

引用次数: 0

Some aspects of biological effect of emodin on plants 大黄素对植物生物学作用的几个方面

Journal of the Belarusian State University. Biology Pub Date : 2021-06-23 DOI: 10.33581/2521-1722-2021-2-97-103

Aliaxandr I. Kakhanouski, V. Yurin, Katsiaryna Yu. Kakhanouskaya

{"title":"Some aspects of biological effect of emodin on plants","authors":"Aliaxandr I. Kakhanouski, V. Yurin, Katsiaryna Yu. Kakhanouskaya","doi":"10.33581/2521-1722-2021-2-97-103","DOIUrl":"https://doi.org/10.33581/2521-1722-2021-2-97-103","url":null,"abstract":"Emodin, a secondary metabolite of many plants, has a wide spectrum of biological action on various groups of organisms, but the mechanisms of its action on plants are practically not studied. There is an assumption that emodin plays a certain role in allelopathy, getting into the soil with fallen leaves in the form of glycosides, which break down into emodin aglycones and are stored in the ecosystem. The aim of the study was to establish the effects of emodin on growth processes and its effect on heme-containing enzymes. It was found that emodin has an inhibitory effect on the root system of Allium cepa, and also promotes the activation of catalase activity in the roots. When studying the direct action of emodin on the activity of the catalase enzyme in vitro, it was found that the activity of the catalase enzyme decreases under the action of emodin. When studying the possible mechanisms of inactivation, it was found that emodin can interact with heme. Further detailing of the patterns of biological action of emodin will expand the scope of its application in plant protection, crop production, and also determine its role in plant ecology and physiology.","PeriodicalId":437681,"journal":{"name":"Journal of the Belarusian State University. Biology","volume":"59 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114834155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of emoxipine on cytotoxicity of peripheral blood mononuclears under cultivation with cytarabine and cyclocytidine 莫西平对阿糖胞苷和环胞苷培养的外周血单核细胞毒性的影响

Journal of the Belarusian State University. Biology Pub Date : 2021-06-23 DOI: 10.33581/2521-1722-2021-2-3-10

D. Nizheharodava, M. Zafranskaya, E. Kvasyuk, A. Sysa

引用次数: 1

The effect of ultraviolet diode radiation on the growth rate of Alternaria colonies 紫外二极管辐射对互花菌菌落生长速率的影响

Journal of the Belarusian State University. Biology Pub Date : 2021-06-23 DOI: 10.33581/2521-1722-2021-2-19-28

Alina A. Rusakovich, A. Y. Shashko, Aliaksei A. Viatoshkin, Aliaksei A. Saldatsenka, Uladzimir V. Balvanovich, Ihar I. Smolich, A. Sokolik, Yuriy K. Shashko, V. Demidchik

{"title":"The effect of ultraviolet diode radiation on the growth rate of Alternaria colonies","authors":"Alina A. Rusakovich, A. Y. Shashko, Aliaksei A. Viatoshkin, Aliaksei A. Saldatsenka, Uladzimir V. Balvanovich, Ihar I. Smolich, A. Sokolik, Yuriy K. Shashko, V. Demidchik","doi":"10.33581/2521-1722-2021-2-19-28","DOIUrl":"https://doi.org/10.33581/2521-1722-2021-2-19-28","url":null,"abstract":"Phytopathogenic micromycetes induce dangerous crop diseases. Traditionally, fungicides have been used to prevent these diseases. In recent years, environmentally friendly non-chemical methods for combating fungal infections have been developed. In particular, ultraviolet (UV) treatment of various wavelengths, intensities and origins has been shown to be effective. In this work, we have analysed the effect of diode low-intensity shortwave UV radiation (UV-C) on the growth and potential viability of the mycelium of Alternaria radicina and A. alternata, the pathogens of important crops. It was shown that irradiation by UV-C diode inhibited growth of Alternaria species at the early stages of development. This effect was high in the first 3 d after UV exposure; however, after 5 d after irradiation, the growth of pathogenic fungi fully restored, suggesting that UV can be only used for partial removal of Alternaria. It was found that A. alternata is less sensitive to diode UV irradiation. The obtained results indicate that the Alternaria micromycetes, particularly, A. alternata, containing high melanin levels, are resistant to low-intensity UV-C diode irradiation.","PeriodicalId":437681,"journal":{"name":"Journal of the Belarusian State University. Biology","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128700509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Study of the ascorbate-glutathione cycle in transgenic plants Nicotiana tabacum under abiotic stress conditions 非生物胁迫条件下转基因烟草抗坏血酸-谷胱甘肽循环的研究

Journal of the Belarusian State University. Biology Pub Date : 2021-06-23 DOI: 10.33581/2521-1722-2021-2-11-18

Kristina V. Pristupa, Tatsiana A. Kukulianskaya, E. Khramtsova

引用次数: 2

The content of plant pigments in bottom sediments of Batorino Lake and their spectral pigment indices 巴托里诺湖底沉积物中植物色素含量及其光谱色素指数

Journal of the Belarusian State University. Biology Pub Date : 2021-06-23 DOI: 10.33581/2521-1722-2021-2-84-96

H. Zhukava, V. S. Smolskaya, Ekaterina A. Vishnevetskaya, J. Karosienė

{"title":"The content of plant pigments in bottom sediments of Batorino Lake and their spectral pigment indices","authors":"H. Zhukava, V. S. Smolskaya, Ekaterina A. Vishnevetskaya, J. Karosienė","doi":"10.33581/2521-1722-2021-2-84-96","DOIUrl":"https://doi.org/10.33581/2521-1722-2021-2-84-96","url":null,"abstract":"Studies on the Batorino Lake were aimed at assessing the quantitative and qualitative characteristics of the autotrophic component of bottom sediments, as well as searching for their relationships with the data of spectral analysis of the pigment composition. The content of total chlorophyll a (without correction for the presence of phaeopigments) was relatively low – 12.7 ± 10.2 µg/g wet weight of bottom sediments. The content of carotenoids averaged 10.24 ± 4.89 µg/g wet weight of bottom sediments. It has been shown that pigment indices: Е450 /Е480, Е480/Е664, Е430/Е664, Е430/Е412, characterise the structural indicators of bottom sediments and reflect the long-term trends in the development of primary producers in water ecosystems, primarily phytoplankton. During the preliminary analysis, auxiliary pigment indices for controlling the purity of the extract and the clarity of the prescribed spectrum were calculated: Е664/Е720, Е430/Е720, Е530/Е720, Е412/Е720, Е430/Е530, Е664/Е530, Е412/Е664. A close inverse relationship was revealed between Е430/Е664 index and the amount of chlorophyll a at different depths of bottom sediments (r = – 0.7), as well as an average correlation between the Е430/ Е412 index and the proportion of phaeopigments (r = – 0.56).","PeriodicalId":437681,"journal":{"name":"Journal of the Belarusian State University. Biology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128646612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0