通过灭活lysR3和mtfA基因,提高油菜假单胞菌S-1的抑菌活性

Anna A. Muratova, M. Mandryk-Litvinkovich, L. Valentovich
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摘要

对甘蓝假单胞菌S-1基因lysR3和mtfA进行了位点特异性无标记失活。结果表明,菌株P. brassicacearum S-1-lysR3、P. brassicacearum S-1-mtfA和P. brassicacearum S-1-lysR3- mtfa对多种植物病原体的拮抗活性比原菌株提高了1.2 ~ 1.6倍。在室内试验中,突变体对番茄、黄瓜和欧芹的生长发育没有影响。用菌株P. brassicacearum S-1-lysR3-mtfA培养液处理番茄和黄瓜种子,幼苗根系长度比原菌株增加了1.4倍。我们还确定所检查的菌株能够在土壤微生物联合体中生存。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Increasing the antimicrobial activity of Pseudomonas brassicacearum S-1 by inactivation of lysR3 and mtfA genes
Site-specific markerless inactivation of genes lysR3 and mtfA of bacteria Pseudomonas brassicacearum S-1 was carried out. It was shown that strains P. brassicacearum S-1-lysR3, P. brassicacearum S-1-mtfA and P. brassicacearum S-1-lysR3-mtfA demonstrate increased antagonistic activity against a number of phytopathogens 1.2–1.6 times higher compared with the original strain. In the laboratory experiment, mutant variants did not affect growth and development of tomato, cucumber and parsley plants. Treating tomato and cucumber seeds with a solution of the culture of the strain P. brassicacearum S-1-lysR3-mtfA led to the increase of seedlings roots length by 1.4 times compared with the original strain. We also determined that the examined strains were able to survive in the soil microbial consortium.
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