Italian Journal of Medicine最新文献_第3页

Prevalence and determinants of permanent atrial fibrillation in post-menopausal hypertensive women 绝经后高血压妇女永久性房颤的患病率和决定因素

Italian Journal of Medicine Pub Date : 2023-10-02 DOI: 10.4081/itjm.2023.1652

Maria Maiello, Annagrazia Cecere, Marco Matteo Ciccone, Pasquale Palmiero

{"title":"Prevalence and determinants of permanent atrial fibrillation in post-menopausal hypertensive women","authors":"Maria Maiello, Annagrazia Cecere, Marco Matteo Ciccone, Pasquale Palmiero","doi":"10.4081/itjm.2023.1652","DOIUrl":"https://doi.org/10.4081/itjm.2023.1652","url":null,"abstract":"Objective. We studied the prevalence and major predisposing factors of permanent atrial fibrillation (PAF) in a population of hypertensive post-menopausal (HPMW) women. Materials and Methods. We enrolled 125 consecutive HPMW with PAF, 125 HPMW in sinus rhythm were the control group (CG). Women with valvular heart disease, coronary artery disease, WPW syndrome, left ventricular (LV) ejection fraction <45% and thyroid disorders were excluded. The mean age was similar: 53±3 years for women on PAF and 51±6 for women with sinus rhythm (P<0.45). All women who underwent M-B mode echocardiography, LV mass, function, and left atrial (LA) volume were assessed according to American Society of Echocardiography guidelines. Diabetes was assessed according to American Diabetes Association guidelines. Results. In a population of 8945 consecutive women, 4497 were hypertensive (50.2%) and 125 were on PAF (0.3%). We observed a highly significant difference between the two groups in relation to obesity: 31 (24.8%) in the AF-group and 15 (12%) in CG, Chi-squared 10, P<0.0016, OR 2.8, 95%; to increased LA volume: 37 (29.6%) in AF-group and 13 (10.4%) in CG, Chi-squared 14.4, P<0.0001, OR 3.62, 95%. No difference in diabetes: 30 (24%) in AFgroup and 28 (22.4%) in CG, Chi-squared 0.09, P<0.76, OR 1.09, 95%, and LV diastolic dysfunction (LVDD): 24 (19.2%) in AF-group and 20 (16%) in CG, Chi-squared 0.44, P<0.5, OR 1.25, 95%. In contrast to what was expected LV hypertrophy had a lower incidence of 59 (47.2%) in AF-group than in CG 84 (67.2%), Chi-squared 10.21, P<0.0014, OR 0.44, 95%. Conclusions. The prevalence of PAF in HPMW is not elevated, obesity and increased LA volume are strong determinants of PAF, not depending on age. LVDD, LV hypertrophy, and diabetes status seem not to play a relevant role as PAF determinants.","PeriodicalId":43715,"journal":{"name":"Italian Journal of Medicine","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135830024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Predictive model for diabetes mellitus occurrence in Iran’s southeastern region: a study based on American diabetes association guidelines 伊朗东南部地区糖尿病发生的预测模型:一项基于美国糖尿病协会指南的研究

Italian Journal of Medicine Pub Date : 2023-09-26 DOI: 10.4081/itjm.2023.1642

Seyedeh Mahdieh Khoshnazar, Hamid Najafipour, Lida SoltaniNejad, Sara Pezeshki, Gholamreza Yousefzadeh

{"title":"Predictive model for diabetes mellitus occurrence in Iran’s southeastern region: a study based on American diabetes association guidelines","authors":"Seyedeh Mahdieh Khoshnazar, Hamid Najafipour, Lida SoltaniNejad, Sara Pezeshki, Gholamreza Yousefzadeh","doi":"10.4081/itjm.2023.1642","DOIUrl":"https://doi.org/10.4081/itjm.2023.1642","url":null,"abstract":"To control diabetes in a society, risk assessment tools are used to predict disease risk. We aimed to assess the value of different risk factors for diabetes mellitus in a remarkable community in the city of Kerman, one of the vast areas in the southeast of Iran, with the final goal of designing a predictive model for diabetes in this region. This study was a cross-sectional study with the aim of investigating the predictive value of risk factors indicating the presence of diabetes in the population of Kerman City based on the guidelines of the American Diabetes Association (ADA) risk assessment tool. The information of 4000 people participating in the comprehensive screening plan for cardiovascular risk factors in Kerman City was extracted by reviewing the relevant data registry. According to the ADA guideline, 32.5% of participants were at risk for diabetes mellitus. The hazard ratio of diabetes mellitus in the subgroup with the ADA final score ≥5 as compared to those with a lower final score was 1.9. Advanced age, history of gestational diabetes, family history of diabetes mellitus, history of hypertension, low physical activity, and higher body mass index were the main determinants of diabetes mellitus. According to ADA guidelines and the diabetes mellitus risk assessment tool, 32.5% of the population residents in Kerman City are potentially at risk for diabetes mellitus that can be successfully predicted aide by the ADA risk assessment tool.","PeriodicalId":43715,"journal":{"name":"Italian Journal of Medicine","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134886360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Collecting clinical data during an emergency: quality of life in primary biliary cholangitis during the COVID-19 pandemic 在紧急情况下收集临床数据:COVID-19大流行期间原发性胆管炎患者的生活质量

Italian Journal of Medicine Pub Date : 2023-09-26 DOI: 10.4081/itjm.2023.1609

Marco Delle Monache, Marco Carli, Annarita Vestri, Lorenzo Nosotti, Teresa Morgillo, Michele Delle Monache

{"title":"Collecting clinical data during an emergency: quality of life in primary biliary cholangitis during the COVID-19 pandemic","authors":"Marco Delle Monache, Marco Carli, Annarita Vestri, Lorenzo Nosotti, Teresa Morgillo, Michele Delle Monache","doi":"10.4081/itjm.2023.1609","DOIUrl":"https://doi.org/10.4081/itjm.2023.1609","url":null,"abstract":"Background. Primary biliary cholangitis (PBC) is a chronic autoimmune liver disease. As an infrequent disease, a Facebook group was created for patients to share experiences and problems. In fact, during the COVID-19 pandemic, patient analysis could only be done through remote connection systems. Therefore, to analyze patients' quality of life (QoL), we exploited social networks and online data collection platforms. Objectives. A survey was carried out to evaluate the QoL of patients with PBC during the COVID-19 pandemic. Materials and Methods. A Facebook group was used for patient enrolment. Age, sex, diagnosis, years since diagnosis, associated diseases, histological stage of the disease, value of elastography, and current therapy were collected. PBC 40 online questionnaire was submitted to patients to assess their QoL. Results. 78 patients participated in the study: 75 females, and 3 males, the mean (±SD) age was 46.4±11.5. The main diagnoses were PBC in 66 patients and overlapping syndrome PBC + autoimmune hepatitis in 10. Histology was available in 45 patients, of whom 34 were stages 1-2 and 11 stages 3-4. The main therapy was ursodeoxycholic acid in 56 pts. The questionnaire is divided into 6 domains, covering fatigue, emotional, social, and cognitive functions, general symptoms, and itching. The mean and standard deviation of the scores were computed. Interpretation of the results obtained by applying a quantitative scale showed no impairment for social, mild impairment for general symptoms, itching, cognitive and emotional function, and moderate impairment for fatigue. No correlation was found between scores and disease duration. Conclusions. This study demonstrates that online questionnaires are a viable substitute for paper questionnaires and that data collected from online surveys on Facebook can have scientific relevance; PBC had the greatest impact on QoL on fatigue and the least on social aspects.","PeriodicalId":43715,"journal":{"name":"Italian Journal of Medicine","volume":"62 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134960305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of knowledge and attitude among healthcare workers towards monkeypox disease: a cross-sectional study from Saudi Arabia 评估卫生保健工作者对猴痘疾病的知识和态度:一项来自沙特阿拉伯的横断面研究

Italian Journal of Medicine Pub Date : 2023-09-26 DOI: 10.4081/itjm.2023.1645

Talal Qadah

{"title":"Assessment of knowledge and attitude among healthcare workers towards monkeypox disease: a cross-sectional study from Saudi Arabia","authors":"Talal Qadah","doi":"10.4081/itjm.2023.1645","DOIUrl":"https://doi.org/10.4081/itjm.2023.1645","url":null,"abstract":"Background. Monkeypox has been announced recently as a global public health emergency by the World Health Organization. The recent outbreak of monkeypox has brought back concerns about how this viral infection can spread easily between individuals and cause a major health concern. Objectives. The objective of this study was to assess healthcare workers’ (HCWs) knowledge about monkeypox illness, its transmission, prevention, and attitudes toward this re-emerging disease. Materials and Methods. A descriptive correlational cross-sectional survey was conducted in Saudi Arabia from June to August 2022 and targeted HCWs including medical technologists and technicians, physicians, nurses, pharmacists, and others. Results. A total of 385 participants were included where 53.5% of respondents were male compared to 46.5% female. The average score for knowledge was 9.90, and the majority of respondents had an inadequate understanding of monkeypox. In addition, the average score of attitude toward monkeypox infection was 50.19, which indicate that HCWs have a positive view of efforts to prevent monkeypox. Conclusions. In Saudi Arabia, HCWs were found to have an inadequate understanding of the virus that causes monkeypox. This study shows how important it is for HCWs to learn more, since managing contagious infections requires a lot of help from trained and knowledgeable medical professionals.","PeriodicalId":43715,"journal":{"name":"Italian Journal of Medicine","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134958284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The mechanism of action of Spirulina as antidiabetic: a narrative review 螺旋藻抗糖尿病作用机制综述

Italian Journal of Medicine Pub Date : 2023-09-20 DOI: 10.4081/itjm.2023.1639

Mohamed A. El-Sakhawy, Mohammad Zahidul Iqbal, Gamal A. Gabr, Abdullah A. Alqasem, Abeer Ali El-Sherbiny Ateya, Fatma A. Ahmed, Samah A. El-Hashash, Heba S. Ibrahim, Usama M. Abu El-Ghiet

{"title":"The mechanism of action of Spirulina as antidiabetic: a narrative review","authors":"Mohamed A. El-Sakhawy, Mohammad Zahidul Iqbal, Gamal A. Gabr, Abdullah A. Alqasem, Abeer Ali El-Sherbiny Ateya, Fatma A. Ahmed, Samah A. El-Hashash, Heba S. Ibrahim, Usama M. Abu El-Ghiet","doi":"10.4081/itjm.2023.1639","DOIUrl":"https://doi.org/10.4081/itjm.2023.1639","url":null,"abstract":"Spirulina happens to be a special type of blue-green algae that originally emerged 3.5 billion years ago and was used as a source of nutrition. Spirulina gets its name from the filaments’ spiral or helical structure, but its true name is taxonomically Genus Arthrospira which encompasses several species. The most common species are S. fusiformis, S. maxima, and S. platensis. It is rich in various nutrients and chemical components including protein, carbohydrates, lipids, vitamins, minerals, pigments, chlorophyll, and enzymes. Spirulina’s active molecules and rich nutrients make it have several pharmacological activities and uses including antioxidant, anti-inflammatory, immunomodulatory, immune system booster, anticancer, antiviral activity, and neuroprotective properties. It is also utilized as a nutritional supplement and for weight loss. Moreover, several studies confirm that Spirulina improves insulin sensitivity and reduces blood glucose levels in rat models as well as diabetic patients. The reason behind this unique behavior could be credited to the presence of several active components in it, but the action’s fundamental mechanism is still a matter of debate. Several studies have suggested different mechanisms including anti-inflammatory activity, increased insulin sensitivity, inhibition of gluconeogenesis, antioxidant activity, modulating gut microbiota composition, improved glucose homeostasis, and insulin receptor activation. Therefore, it became clear that Spirulina is a mine of active substances used as a nutritional supplement and reduces blood glucose levels or used in conjunction with other treatments to tackle type 2 diabetes. Further exploration is required to fully explain its effects on human physiology and determine optimal dosages for treatment.","PeriodicalId":43715,"journal":{"name":"Italian Journal of Medicine","volume":"67 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136308171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A review on management of antiphospholipid syndrome in clinical practice 抗磷脂综合征的临床治疗综述

Italian Journal of Medicine Pub Date : 2023-09-13 DOI: 10.4081/itjm.2023.1649

Carmine Siniscalchi, Pietro Rossetti, Gaetano Carolla, Pierpaolo Di Micco, Adriano Stella, Michele Riva

{"title":"A review on management of antiphospholipid syndrome in clinical practice","authors":"Carmine Siniscalchi, Pietro Rossetti, Gaetano Carolla, Pierpaolo Di Micco, Adriano Stella, Michele Riva","doi":"10.4081/itjm.2023.1649","DOIUrl":"https://doi.org/10.4081/itjm.2023.1649","url":null,"abstract":"Antiphospholipid syndrome (APS) is a systemic autoimmune disease characterized by thrombotic or obstetric events occurring in patients with persistent antiphospholipid antibodies. Thrombotic APS is characterized by venous, arterial, or microvascular thrombosis. The diagnosis is accepted when both one clinical and one laboratory criteria according to the updated Sapporo classification are established. APS may occur in combination with other autoimmune diseases, mainly systemic lupus erythematosus, or in its primary form. Long-term anticoagulation with a vitamin K antagonist is the standard of care for patients who develop thrombosis, considering the high rate of recurrent thrombosis. The current international guidelines are not in favor of recommending direct oral anticoagulants for secondary prevention of thrombotic antiphospholipid syndrome, especially in the context of arterial thrombosis and triple-positive antiphospholipid patients. The most common approach, endorsed by the American College of Chest Physicians guidelines is the combination of heparin and low-dose aspirin (75-100 mg) daily for women who fulfill the clinical and serologic criteria for obstetric APS. New potential therapeutic approaches are under evaluation but actually the anticoagulation remains the cornerstone of treatment.","PeriodicalId":43715,"journal":{"name":"Italian Journal of Medicine","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135741945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of methionine synthase reductase (MTRR A66G) polymorphism with susceptibility to acute lymphoblastic leukemia 蛋氨酸合成酶还原酶(MTRR A66G)多态性与急性淋巴细胞白血病易感性的关系

Italian Journal of Medicine Pub Date : 2023-09-13 DOI: 10.4081/itjm.2023.1644

Mashaer Taha Edris, Maye Mohammad Merghani, Shaza Salih Gafar, Ahmed Mohamed Asmali, Elrashed B. Yasin, Raed Alserihi, Heba Alkhatabi, Haitham MH. Qutob, Rowaid Qahwaji, Elshazali Widaa Ali

{"title":"Association of methionine synthase reductase (MTRR A66G) polymorphism with susceptibility to acute lymphoblastic leukemia","authors":"Mashaer Taha Edris, Maye Mohammad Merghani, Shaza Salih Gafar, Ahmed Mohamed Asmali, Elrashed B. Yasin, Raed Alserihi, Heba Alkhatabi, Haitham MH. Qutob, Rowaid Qahwaji, Elshazali Widaa Ali","doi":"10.4081/itjm.2023.1644","DOIUrl":"https://doi.org/10.4081/itjm.2023.1644","url":null,"abstract":"Background and Objectives. The enzyme methionine synthase reductase is involved in cellular methylation reactions, DNA synthesis, and epigenetic processes. It is encoded by the MTRR gene, which garnered a lot of attention in current medical genetics research. This study was conducted to study the association between MTRR (A66G) polymorphism and the risk of developing acute lymphoblastic leukemia among Sudanese patients. Materials and Methods. This is a case-control study in which 150 patients with acute lymphoblastic leukemia (ALL) and 150 healthy participants as a control group were enrolled. DNA was extracted and analyzed for the MTRR (A66G) polymorphism using the real-time polymerase chain reaction. Results. Based on flow cytometry results, B-ALL was more common (79%) than T-ALL (21%). The comparison of hematological parameters in acute lymphoblastic leukemia subtypes showed a statistically significant high mean total white blood count (P=0.000) and mean blast percentage (P=0.050) in patients with T-ALL. The molecular analysis showed that the incidence of the MTRR homozygous genotypes AA and GG were higher in the patients (44% and 9.3%, respectively) compared to the control group (40% and 6.7%, respectively). In comparison, the heterozygous genotype AG was lower in the patients (46.7%) than in the control group (53.3%). However, the association between the polymorphism and acute lymphoblastic leukemia risk was not statistically significant (OR: 1.179, 95% CI 0.7459-1.865, P=0.445). Conclusions. This study concluded that MTRR A66G polymorphism was not associated with the risk of acute lymphoblastic leukemia among the Sudanese population.","PeriodicalId":43715,"journal":{"name":"Italian Journal of Medicine","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135742010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of hub genes associated with hepatitis B virus-related hepatocellular cancer using weighted gene co-expression network analysis and protein-protein interaction network analysis 利用加权基因共表达网络分析和蛋白-蛋白相互作用网络分析鉴定与乙型肝炎病毒相关的肝细胞癌相关的枢纽基因

IF 0.4

Italian Journal of Medicine Pub Date : 2023-09-06 DOI: 10.4081/itjm.2023.1626

Wenze Wu, Fang Lin, Zifan Chen, Kejia Wu, Changhuan Ma, Zhuang Jing, Donglin Sun, Qiang Zhu, Longqing Shi

{"title":"Identification of hub genes associated with hepatitis B virus-related hepatocellular cancer using weighted gene co-expression network analysis and protein-protein interaction network analysis","authors":"Wenze Wu, Fang Lin, Zifan Chen, Kejia Wu, Changhuan Ma, Zhuang Jing, Donglin Sun, Qiang Zhu, Longqing Shi","doi":"10.4081/itjm.2023.1626","DOIUrl":"https://doi.org/10.4081/itjm.2023.1626","url":null,"abstract":"Background. Chronic hepatitis B virus (HBV) infection is the main pathogen of hepatocellular carcinoma. However, the mechanisms of HBV-related hepatocellular carcinoma (HCC) progression are practically unknown. Materials and Methods. The results of RNA-sequence and clinical data for GSE121248 and GSE17548 were accessed from the Gene Expression Omnibus data library. We screened Sangerbox 3.0 for differentially expressed genes (DEGs). The weighted gene co-expression network analysis (WGCNA) was employed to select core modules and hub genes, and protein-protein interaction network module analysis also played a significant part in it. Validation was performed using RNA-sequence data of cancer and normal tissues of HBV-related HCC patients in the cancer genome atlas-liver hepatocellular cancer database (TCGA-LIHC). Results. 787 DEGs were identified from GSE121248 and 772 DEGs were identified from GSE17548. WGCNA analysis indicated that black modules (99 genes) and grey modules (105 genes) were significantly associated with HBV-related HCC. Gene ontology analysis found that there is a direct correlation between DEGs and the regulation of cell movement and adhesion; the internal components and external packaging structure of plasma membrane; signaling receptor binding, calcium ion binding, etc. Kyoto Encyclopedia of Genes and Genomes pathway analysis found out the association between cytokine receptors, cytokine-cytokine receptor interactions, and viral protein interactions with cytokines were important and HBV-related HCC. Finally, we further validated 6 key genes including C7, EGR1, EGR3, FOS, FOSB, and prostaglandin-endoperoxide synthase 2 by using the TCGALIHC. Conclusions. We identified 6 hub genes as candidate biomarkers for HBV-related HCC. These hub genes may act as an essential part of HBV-related HCC progression.","PeriodicalId":43715,"journal":{"name":"Italian Journal of Medicine","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47230261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

La progressione della malattia renale cronica 慢性肾病的进展

IF 0.4

Italian Journal of Medicine Pub Date : 2023-09-04 DOI: 10.4081/itjm.q.2023.3

D. Manfellotto, G. Pinna

引用次数: 0

Smad2/3 signaling involved in urotensin II-induced phenotypic differentiation, collagen synthesis and migration of rat aortic adventitial fibroblasts Smad2/3信号参与尿紧张素ii诱导的大鼠主动脉内皮成纤维细胞的表型分化、胶原合成和迁移

IF 0.4

Italian Journal of Medicine Pub Date : 2023-08-30 DOI: 10.4081/itjm.2023.1637

Weizhao Lin, Xu Yang, F. Zheng, Jian-Fei Yang, Yonggang Zhang

{"title":"Smad2/3 signaling involved in urotensin II-induced phenotypic differentiation, collagen synthesis and migration of rat aortic adventitial fibroblasts","authors":"Weizhao Lin, Xu Yang, F. Zheng, Jian-Fei Yang, Yonggang Zhang","doi":"10.4081/itjm.2023.1637","DOIUrl":"https://doi.org/10.4081/itjm.2023.1637","url":null,"abstract":"Objective. To investigate whether Smad2/3 signaling is involved in urotensin II (UII) induced activation of aortic adventitial fibroblasts. Materials and Methods. Growth-arrested adventitial fibroblasts were stimulated with UII in the presence or absence of urotensin II receptor (UT) antagonist SB710411 or transfected with Smad2/3 small inhibitory RNA (siRNA). UII stimulated Smad2/3 phosphorylation, α-smooth muscle actin (α-SMA), and collagen I expression and migration of adventitial fibroblasts were evaluated by western blot analysis, real-time reverse transcription polymerase chain reaction, immunofluorescence, ELISA, and transwell migration assay, respectively. Results. In cultured adventitial fibroblasts, UII time- and dose-dependently stimulated Smad2/3 protein phosphorylation, with maximal effect at 10-8 mol/l (increased by 147.2%, P<0.001). UII stimulated Smad2/3 upregulation and nuclear translocation. SB710411 significantly inhibited these effects. In addition, UII potently induced α-SMA and procollagen 1 protein or mRNA expression (P<0.01), which were completely blocked by Smad2 (decreased by 75.1%, 54.2% in protein, and by 73.3% and 38.2% in mRNA, respectively, P<0.01) or Smad3 siRNA (decreased by 80.3% and 47.0% in protein, and by 72.3% and 47.7% in mRNA, respectively, P<0.01). Meanwhile, Smad2 or smad3 siRNA significantly inhibited the UII-induced collagen 1 secretion and cell migration. Conclusions. UII may stimulate adventitial-fibroblast phenotype conversion, migration, and collagen I synthesis via phosphorylated-Smad2/3 signal transduction pathways.","PeriodicalId":43715,"journal":{"name":"Italian Journal of Medicine","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45220701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0