Bioremediation Science and Technology Research最新文献_第2页

A Two-level Factorial Design for Screening Factors that Influence the Growth of Staphylococcus sp. strain Amr-15 on Acrylamide 影响葡萄球菌Amr-15在丙烯酰胺上生长的因素筛选的两水平析因设计

Bioremediation Science and Technology Research Pub Date : 2022-12-31 DOI: 10.54987/bstr.v10i2.777

M. F. Rahman, M. E. Khayat, M. El-Mongy, H. Yakasai

{"title":"A Two-level Factorial Design for Screening Factors that Influence the Growth of Staphylococcus sp. strain Amr-15 on Acrylamide","authors":"M. F. Rahman, M. E. Khayat, M. El-Mongy, H. Yakasai","doi":"10.54987/bstr.v10i2.777","DOIUrl":"https://doi.org/10.54987/bstr.v10i2.777","url":null,"abstract":"Due to the fact that it breaks down into acrylamide over time, polyacrylamide is one of the most important sources of acrylamide in soil. As a strategy for bioremediation, the breakdown of acrylamide by the action of microbes has seen a gradual but consistent increase in attention all over the world. A previously isolated molybdenum-reducing bacterium with amide-degrading capability was further identified on significant parameters contributing to optimized growth on acrylamide using a two-level factorial design in this study. The two-level factorial design was adopted in the screening of five independent factors influencing the growth of the bacterium on acrylamide. These factors include pH, temperature, incubation time, acrylamide concentration and glucose concentration. The two-factor factorial design was successful in finding important contributing parameters in the growth of this bacterium on acrylamide, which were acrylamide concentration, pH and incubation time (p<0.05) that can be further optimized using RSM in future works. The important contributing factors or parameters were analysed using ANOVA, Pareto’s chart and perturbations plot and other diagnostic plots. The diagnostic plots such as half-normal, Cook’s distance, residual vs runs, leverage vs runs, Box-Cox, DFFITS, DFBETAS all supported the two-level factorial conclusion. This study was carried out using an acrylamide range well within the range reported to be tolerated by most acrylamide-degrading microorganisms. Incubation time is an expected result since longer incubation time allows more growth and incubation time ranging from two to five days for optimized growth has been reported in many acrylamide-degrading microorganisms. Most of the acrylamide-degrading microorganisms grow well in near-neutral conditions, of which the results obtained in this study conforms to the published literature trends.","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133094093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mathematical Modeling of Substrate Inhibition Kinetics of Staphylococcus aureus Growth on Basic Violet 3 底物抑制金黄色葡萄球菌生长动力学的数学模型

Bioremediation Science and Technology Research Pub Date : 2022-12-31 DOI: 10.54987/bstr.v10i2.790

M. Manogaran, Nur Muhamad Syahir Abdul Habib, M. Shukor, N. A. Yasid

{"title":"Mathematical Modeling of Substrate Inhibition Kinetics of Staphylococcus aureus Growth on Basic Violet 3","authors":"M. Manogaran, Nur Muhamad Syahir Abdul Habib, M. Shukor, N. A. Yasid","doi":"10.54987/bstr.v10i2.790","DOIUrl":"https://doi.org/10.54987/bstr.v10i2.790","url":null,"abstract":"Crystal violet or gentian violet or basic violet 3 (BV) is an essential dye utilized as a dye for textiles and paper, as well as being an ingredient in inks used for printing, ballpoint pens, and inkjet printers. In some cases, it is utilized for the purpose of imparting color to a variety of items, including antifreeze, fertilizer, detergent, and leather. The use of microorganisms for the purpose of BV bioremediation is becoming increasingly common. A number of secondary models, including Monod, Haldane, Teissier, Aiba, Yano and Koga, Hans-Levenspiel, Webb, and the Luong model, can be used to estimate the rate of decolorization, which is frequently blocked at high concentrations of toxicant. These models can be used to simulate the process. The best model based on statistical analysis was Teissier with the highest value for the adjusted coefficient of determination and the lowest values for RMSE, AICc and the closest value to 1.0 for accuracy and bias factors. The Teissier model was found to conform to normality tests and is adequate to be used to fit the experimental data. The experimental data obtained indicates that BV is toxic and slows down the rate of decolourisation at higher concentrations. The maximum BV specific biodegradation rate (qmax), half-saturation concentration (KS), half inhibition concentration (Ki) was 0.145 h-1, 0.408 mg/L and 73.205 mg/L, respectively.","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115249781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Growth on Sodium Dodecyl Sulphate (SDS) by a Bacterial Consortium Isolated from Volcanic Soil 火山土细菌群在十二烷基硫酸钠(SDS)上的生长

Bioremediation Science and Technology Research Pub Date : 2022-12-31 DOI: 10.54987/bstr.v10i2.776

.. Rusnam, S. Syafrawati, M. F. Rahman, Fachri Ibrahim Nasution, Mohd Ezuan Khayay

{"title":"Growth on Sodium Dodecyl Sulphate (SDS) by a Bacterial Consortium Isolated from Volcanic Soil","authors":".. Rusnam, S. Syafrawati, M. F. Rahman, Fachri Ibrahim Nasution, Mohd Ezuan Khayay","doi":"10.54987/bstr.v10i2.776","DOIUrl":"https://doi.org/10.54987/bstr.v10i2.776","url":null,"abstract":"During biodegradation, microorganisms can directly metabolize surfactants for energy and nutrients or co-metabolize them with other compounds. Maximum growth of the bacterial consortium on SDS was seen between 30 and 35 °C, while the optimal pH range for bacterial consortium growth was between 6.5 and 7.5. As for the nitrogen source, 2 g/L of ammonium sulfate was optimum in supporting the growth of SDS. The greatest growth rate of the bacterial consortium was recorded at a concentration of between 1 and 1.5 g/L of SDS (p<0.05). At 2–3, g/L of SDS, the bacterial consortium grew more slowly, and at 5 g/L, growth was severely inhibited. Almost complete degradations of SDS were observed in 3, 5 and 6 days at 0.5, 0.75 and 1 g/L SDS, respectively while higher concentrations showed partial degradation with no degradation observed at 2.5 g/L SDS after 6 days of incubation. In this study, the maximum growth rate, or max, Ks, and Ki were 0.517 h-1 (95% confidence interval of C.I. from 0.404 to 0.629), 0.132 (g/L) (95% C.I. from 0.073 to 0.191) and 0.909 (g/L) (95% C.I. from 0.544 to 1.273), respectively. Heavy metals like mercury, copper, and chromium can severely stunt growth if they are present in the environment. It was discovered through research into growth kinetics that Haldane substrate inhibition kinetics may be used to model the growth rate. This bacterial consortium has the right properties for the bioremediation of SDS-polluted environments.","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133995080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Limits of Detection Determination of Aflatoxin B1 using the Optical Waveguide Lightmode Spectroscopy via the Four-Parameter Logistic Model 基于四参数Logistic模型的光波导光模光谱法测定黄曲霉毒素B1的检出限

Bioremediation Science and Technology Research Pub Date : 2022-12-31 DOI: 10.54987/bstr.v10i2.780

G. Uba, H. Yakasai, A. Abubakar

{"title":"Limits of Detection Determination of Aflatoxin B1 using the Optical Waveguide Lightmode Spectroscopy via the Four-Parameter Logistic Model","authors":"G. Uba, H. Yakasai, A. Abubakar","doi":"10.54987/bstr.v10i2.780","DOIUrl":"https://doi.org/10.54987/bstr.v10i2.780","url":null,"abstract":"Mycotoxins are harmful secondary metabolites generated by a variety of fungi, and they may be found in a vast array of food and feed commodities and processed meats from animals fed infected meal. Numerous mycotoxins are extremely resistant and survive food processing, entering the food chain and posing a concern to human health. The \"optical waveguide lightmode spectroscopy\" (OWLS) method was used to detect aflatoxin B1 in plant sample matrices. The calibration curve for the detection of aflatoxin B1 utilizing \"optical waveguide lightmode spectroscopy\" (OWLS) displayed a sigmoidal shape; hence, the 5-PL or 4-PL model should be used to fit the data rather than a linear model. Using error function analysis with functions such as AICc, HQC, BIC, RMSE, adjR2, Bias Factor, and Accuracy Factor, the 5-PL and 4-Pl models are distinguished inconsistently. The overlapping confidence intervals of the LogEC50 values suggested that the two techniques did not differ much, and the 4-PL model was selected due to its smaller number of parameters. The Limits of Detection for aflatoxin B1 value based on the 4-PL equation was 8.787 ng/mL with the 95% confidence interval from 5.728 to 13.100. In this study, the use of the 4-PL model was successful and was able to represent the entire date curve, not only the linear section. The linear component is crucial as a handy and swift approach for assessing the sensitivity of a developed biosensor technology and is often a more beneficial method for field applications when a quick and straightforward evaluation is required.","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129062054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Immobilization of Bacillus sp. Strain Neni-8 in Dialysis Tubing Reduced Copper Toxicity to the Molybdenum Reduction Process 在透析管中固定化芽孢杆菌ni-8可降低铜对钼还原过程的毒性

Bioremediation Science and Technology Research Pub Date : 2022-12-31 DOI: 10.54987/bstr.v10i2.781

G. Uba, A. Abubakar, H. Yakasai

{"title":"Immobilization of Bacillus sp. Strain Neni-8 in Dialysis Tubing Reduced Copper Toxicity to the Molybdenum Reduction Process","authors":"G. Uba, A. Abubakar, H. Yakasai","doi":"10.54987/bstr.v10i2.781","DOIUrl":"https://doi.org/10.54987/bstr.v10i2.781","url":null,"abstract":"In ruminants, even trace amounts of molybdenum can be lethal. In areas with high pollution, molybdenum levels in soil and mine tailings can exceed 20,000 ppm. Bioremediation of molybdenum can be challenging when toxic copper is also present. This research presents a novel approach using dialysis tubing and the molybdenum-reducing activity of Bacillus sp. strain Neni-8 for molybdenum removal from aqueous solutions. Molybdenum blue (Mo-blue), produced during enzymatic reduction, is insoluble in dialysis tubing and this can be a twofold advantage as a method of removal and as a method to protect bacterial cells from heavy metal inhibition, especially copper. In this experiment, we assess the toxicity-shielding effect of dialysis tubing for molybdenum reduction to Mo-blue by this bacterium in the presence of copper. As the concentrations of copper were increased, both free and immobilized cells were strongly inhibited. Modelling using the dissociationone-phase exponential decay model gave an IC50 value for the immobilized form of 0.1107 mg/L (95% confidence interval from 0.073 to 0.217 while the IC50 value for the free cell system was 0.023 mg/L (95% C.I. from 0.019 to 0.028). Since the confidence interval for the IC50 values did not overlap, the immobilized system gave better protection from copper than the free cell system. Toxicity to free cells was higher than toxicity to cells trapped in dialysis tubes, suggesting that trapping Mo-reducing cells may be an effective strategy for the bioremediation of water or wastewater contaminated with multiple heavy metals.","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123607278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antioxidant Vitamins and Minerals Composition of Fresh Carrot-Cabbage Juice 新鲜胡萝卜-卷心菜汁的抗氧化维生素和矿物质组成

Bioremediation Science and Technology Research Pub Date : 2022-07-31 DOI: 10.54987/bstr.v10i1.682

Abbas Sani, Sanusi Sani

{"title":"Antioxidant Vitamins and Minerals Composition of Fresh Carrot-Cabbage Juice","authors":"Abbas Sani, Sanusi Sani","doi":"10.54987/bstr.v10i1.682","DOIUrl":"https://doi.org/10.54987/bstr.v10i1.682","url":null,"abstract":"Antioxidants are substances that protect cells from the damages caused by free radicals. Fruit and vegetables are rich in antioxidants and provide important sources of antioxidants. While the quest for an antioxidant supplement is ongoing, this study investigated the antioxidant vitamins and minerals composition of fresh carrot-cabbage juice. Antioxidant vitamins and minerals compositions of the juice were determined by standard methods using spectrophotometry and atomic absorption spectrophotometry (AAS) respectively. The concentration of antioxidant vitamins was found to be in the order; vitamin C > vitamin A > vitamin E. However, manganese has the highest concentration (P < 0.05) among minerals. There is no significant difference between the concentrations of manganese and copper (P > 0.05). The concentration of chromium is significantly lower (P < 0.05) than that of manganese and copper but greater (P < 0.05) than that of zinc. The study showed that fresh carrot-cabbage juice contains a respective concentration of antioxidant vitamins (A, C and E) and antioxidant minerals (Cu, Cr, Mn and Zn). The juice is hence a good source of antioxidant vitamins and minerals and may help complement the antioxidant system of the body.","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"111 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116765888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Test of the Randomness of Residuals and Detection of Potential Outliers for the Modified Gompertz Model Used in the Fitting of the Growth of Shigella flexneri 修正Gompertz模型用于拟合福氏志贺氏菌生长的残差随机性检验和潜在异常值检测

Bioremediation Science and Technology Research Pub Date : 2022-07-31 DOI: 10.54987/bstr.v10i1.687

G. Uba, Bilal Ibrahim Dan-Iya

{"title":"Test of the Randomness of Residuals and Detection of Potential Outliers for the Modified Gompertz Model Used in the Fitting of the Growth of Shigella flexneri","authors":"G. Uba, Bilal Ibrahim Dan-Iya","doi":"10.54987/bstr.v10i1.687","DOIUrl":"https://doi.org/10.54987/bstr.v10i1.687","url":null,"abstract":"The formulation of hypotheses and the recommendation of experiments as the subsequent stages of the research process are both brought about as a result of the utilization of complicated computer models that make it possible to represent intricate biological processes. Because these systems rely on random data, this is a necessity for all parametric statistical assessment procedures. When the diagnostic tests reveal that the residuals make up a pattern, there are a few different treatment choices available to choose from. Two of these alternatives include running a nonparametric analysis or switching to a new model. In this study, we use the Wald-Wolfowitz runs test as a statistical diagnosis tool to determine whether or not the randomization conditions have been met. The runs test found that there were 5 total runs, although the randomness assumption predicted 7.46 runs. The null hypothesis is not rejected since the p-value is greater than 0.05; this suggests that there is no convincing evidence of the residuals' non-randomness; rather, the residuals represent noise. In addition, the Grubb’s outlier test shows no indication of an outlier, further corroborate the scenario of the adequacy of the modified Gompertz model used in the fitting of the growth of Shigella flexneri.","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114368741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mathematical Isothermal Modeling of Remazol Black B Biosorption by Aspergillus flavus 黄曲霉吸附雷马唑黑B的等温数学模型

Bioremediation Science and Technology Research Pub Date : 2022-07-31 DOI: 10.54987/bstr.v10i1.685

Salihu Yahuza, I. Sabo, Abdussamad Abubakar, Bilal Ibrahim Dan-Iya

{"title":"Mathematical Isothermal Modeling of Remazol Black B Biosorption by Aspergillus flavus","authors":"Salihu Yahuza, I. Sabo, Abdussamad Abubakar, Bilal Ibrahim Dan-Iya","doi":"10.54987/bstr.v10i1.685","DOIUrl":"https://doi.org/10.54987/bstr.v10i1.685","url":null,"abstract":"Azo dyes, like Remazol Black B, form covalent bonds with textile fibers like cotton, setting them apart from traditional dyes. Due to their advantageous qualities of vivid color, water resistance, straightforward application processes, and low energy consumption, they are widely used in the textile industry. Major environmental effects of their discharge into receiving streams include reduced photosynthesis in aquatic life as a result of decreased light penetration. Seven isotherm models—Henry, Langmuir, Freundlich, BET, Toth, Fritz-Schlunder IV, and Fritz-Schlunder V—were used to analyze the biosorption isotherm data of Remazol Black B dye biosorption by Aspergillus flavus and were fitted using non-linear regression. Based on statistical analysis, the Fritz-Schlunder IV was determined to be the best model using root-mean-square error (RMSE), adjusted coefficient of determination (adjR2), bias factor (BF), accuracy factor (AF), corrected AICc (Akaike Information Criterion), Bayesian Information Criterion (BIC), and Hannan-Quinn information criterion (HQC). The calculated Fritz-Schlunder IV parameter, bFS value was found to be 3.812 mg/g (95% confidence interval of 0.312 to 7.311) and qmFS value of 0.0224 (95% confidence interval of -21725.002 to 21725.047).","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132798005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clay Characterization and Bleaching of Crude Palm Oil Using Acid-Activated Nibo Clay 酸活化Nibo粘土对粗棕榈油的漂白及粘土表征

Bioremediation Science and Technology Research Pub Date : 2022-07-31 DOI: 10.54987/bstr.v10i1.683

C. N. Nweke, R. Ajemba

{"title":"Clay Characterization and Bleaching of Crude Palm Oil Using Acid-Activated Nibo Clay","authors":"C. N. Nweke, R. Ajemba","doi":"10.54987/bstr.v10i1.683","DOIUrl":"https://doi.org/10.54987/bstr.v10i1.683","url":null,"abstract":"Acid-activated Nibo clay was applied as an adsorbent in the bleaching of crude palm oil. The collected clay sample was activated with hydrochloric acid solution after it was sun-dried and ground. The raw (NC) and activated clay (ANC) samples were characterized using XRF, SEM, and FTIR analyses. The efficiency of the bleaching process was examined by varying the adsorbent dosage, temperature and the contact time. The results of XRF, SEM, and FTIR analyses on NC and ANC showed that the clays were kaolinites with significant changes after activation. The bleaching experiment showed that increase in temperature improved the efficiency of the process. The highest bleaching efficiency of 86% was observed. The pseudo-second-order model best described the adsorption process at 100 °C when compared to correlation coefficient values of the pseudo-first-order, Intra-particle diffusion and Elovich kinetic models. The Temkin isotherm model best fitted the experimental data than the Langmuir, Freundlich and Dubinin-Radushkevich models with R2 values of >0.9 at all temperatures. The enthalpy and entropy values were evaluated as 54,943.90 J/mol and 154.6321 J/mol respectively. The enthalpy and entropy values indicated that the adsorption process was endothermic and showed an increase in randomness at the solid/liquid interface. The negative values of the Gibb’s free energy at 363 and 373 K implied that beta carotene adsorption of crude palm oil at these temperatures was spontaneous and feasible. This experimental study showed that acid activated Nibo clay can be applied as an adsorbent for the bleaching of crude palm oil at higher temperatures. \u0000","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122686919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Feedstock Type on Biostimulation Efficiency and Microbial Community Structure during Biochar-Facilitated Remediation of Petroleum Contaminated Soil 生物质炭促进石油污染土壤修复过程中原料类型对生物刺激效率和微生物群落结构的影响

Bioremediation Science and Technology Research Pub Date : 2022-07-31 DOI: 10.54987/bstr.v10i1.668

Anwuli U. Osadebe, Goodness C. Uzoma, C. Ogugbue, G. Okpokwasili

{"title":"Effect of Feedstock Type on Biostimulation Efficiency and Microbial Community Structure during Biochar-Facilitated Remediation of Petroleum Contaminated Soil","authors":"Anwuli U. Osadebe, Goodness C. Uzoma, C. Ogugbue, G. Okpokwasili","doi":"10.54987/bstr.v10i1.668","DOIUrl":"https://doi.org/10.54987/bstr.v10i1.668","url":null,"abstract":"This study investigated the effect of plant feedstock– and animal feedstock–derived biochar at 10%w/w and 15%w/w amendment levels on the biostimulation efficiency and the cultivable microbial community in the soil during biochar-facilitated remediation of petroleum contaminated soil using standard techniques. Biostimulation was most effective with the animal-based biochar (ABB) treatment while total petroleum hydrocarbon (TPH) removal was greatest in the plant-based biochar (PBB) amended soil. Observed mean TPH levels on Day 60 ranged from about 7000 mg/kg – 7800 mg/kg for the PBB and 11000 mg/kg – 14000 mg/kg for ABB representing removal levels of roughly 51.0%, 57.7%, 72.4% and 73.7% in 10% ABB, 15% ABB, 10% PBB and 15% PBB amended contaminated soils respectively. The cultivable bacterial diversity for both feedstock types shifted from the combination of Firmicutes, Actinobacteria and Proteobacteria phyla at the onset of the study to predominantly Proteobacteria by the end of the study with a distinct reduction in diversity observed with increasing contact time. The dominant cultivable heterotrophic bacterial isolates were Bacillus spp., Pseudomonas spp. and Staphylococcus aureus for ABB and Pseudomonas spp., Klebsiella pneumoniae and Bacillus spp. for PBB. Amongst the cultivable hydrocarbon utilising bacteria obtained, Klebsiella pneumoniae, Pseudomonas spp. and Enterobacter spp. dominated. There were significant differences in TCHB and CHUB abundance and TPH removal efficiency between PBB and ABB amendments at 95% confidence interval. The study established that application of biochar effectively manages petroleum pollutants in soil by stimulating the proliferation and activities of relevant degradative species.","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"73 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129419099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1