Khalij-Libya Journal of Dental and Medical Research最新文献

{"title":"3-Layer Immunoperoxidase Protocol Reaction, Endomucin, and Alpha-smooth Muscle Actin Detection","authors":"Rukaia Sheneeb, Fawzia Takala","doi":"10.47705/kjdmr.216110","DOIUrl":"https://doi.org/10.47705/kjdmr.216110","url":null,"abstract":"Background and objectives. Immunohistochemistry (IHC) is the detection of antigens in tissue sections by specific antibodies. It has the unique advantage over other methods for detection proteins like α-Smooth Muscle Actin and endomucin, enabling the correlation of antigens with their location within a tissue. The aim of the study was to identify α-SMA and endomucin in cardiac muscle tissue and arterial blood vessels which have important diagnostic purposes. Methods. Three Specimens (aSMA, Endomucin and control) of formalin-fixed paraffin embedded mouse embryo tissue sections have been de-waxed, rehydrated. Then they were covered with accurate primary antibody: αSMA slide in dilute mouse monoclonal anti-smooth muscle actin, Endomucin slide in dilute rat monoclonal anti-endomucin and the control slide gets just PBS (no-primary control). Then the sections were covered with the right secondary antibody: αSMA slide with biotinylated rabbit anti-mouse IgGIgG diluted and Endomucin slide with biotinylated rabbit anti-rat IgG, control slide with either antibody. Next color reagent was applied; it contains 3,3′-Diaminobenzidine and 0.3% hydrogen peroxide. Finally examine slides using a microscope. Results. The results showed that there was different brown 3,3′-Diaminobenzidine staining patterns in the two test slides for the individual primary antibodies. The 3,3′-Diaminobenzidine Staining was highly expressed in the external part of the section as a result of the presence of α-Smooth Muscle Actin. Whereas, in case of Endomucin, the stain is expressed in the central part of specimens due to presence of the endothelial tissue. and no staining in the primary control sections. Conclusion. As a result of the presence of α-Smooth Muscle Actin in the muscular tissue,3,3′-Diaminobenzidine Staining was highly expressed in the external part of the section. However, in case of Endomucin the stain is expressed in the central part of specimens due to presence of the endothelial tissue.","PeriodicalId":436335,"journal":{"name":"Khalij-Libya Journal of Dental and Medical Research","volume":"561 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132015227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Association Between Coronary Artery Disease and Type 2 Diabetes Mellitus in Libyan Adults: A Cross-Sectional Study","authors":"Razk Abdalgwad, Ali Fadhlullah, Ahmed Balha","doi":"10.47705/kjdmr.216111","DOIUrl":"https://doi.org/10.47705/kjdmr.216111","url":null,"abstract":"Background and objectives. Diabetes mellitus is a highly prevalent chronic multisystem disease and it has a significant impact on the health of many important organs in our body, including the cardiovascular system (CVS). This study aimed to determine the association between Type 2 diabetes mellites (T2DM) and the development of coronary artery disease (CAD). Methods. A retrospective cross-sectional study was conducted on patients who were diagnosed with CAD and T2DM. We examined the likelihood occurrence of CAD in T2DM patients using the Bayesian one sample test. Results. About 97 patients were confirmed to have CAD and were included in the study. The mean age of participants was 60±11.89. Approximately, 53 (54.6%) of patients were females, while 44 (45.4%) were males. The vast majority of patients had T2DM and CAD at 69 (71.1%) as opposed to only 28 (28.9%) of patients who did not have T2DM but had CAD. Moreover, there was a statistically significant increase in the likelihood of occurrence of CAD amongst the T2DM group. The percentage of patients who have T2DM with CAD was significantly higher than those who were not diagnosed with T2DM but had CAD at 69 (71.1%) compared to 28 (28.9%) (P = 0.002) for the group of T2DM concomitant with CAD, and the group of non-T2DM concomitant with CAD, respectively. Conclusion. There was a significant relationship between T2DM and CAD, suggesting the importance of intensive glycemic control in diabetic patients possibly via lifestyle modifications or medications in order to reduce or delay the possibility of occurrence of CAD amongst diabetic patients, in particular.","PeriodicalId":436335,"journal":{"name":"Khalij-Libya Journal of Dental and Medical Research","volume":"73 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115122335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Final Height Outcome in Girls with Turner Syndrome Treated with Growth Hormone and Low Dose Estrogen","authors":"","doi":"10.47705/kjdmr.216109","DOIUrl":"https://doi.org/10.47705/kjdmr.216109","url":null,"abstract":"We reported final height data of collected 28 cases with Turner Syndrome (TS) were confirmed with chromosomal analysis during height growth hormone therapy and late estrogen therapy. Participants were allocated into one of 3 Groups, Group A: 11 patients aged between 15-18 years-old, group B: 10 patients aged between 10 -15 years-old, group C: 7 patients younger than 10 years-old. In contrast to the duration of growth hormone & presentation, group B showed a significantly higher final height mean (147.5 ±6.5 cm) the best result well be obtained in group B treated with high doses of growth hormone for longer period. Records of female patients with TS confirmed by karyotype analysis were examined; 15 patients had the typical Turner karyotype and 13 patients had a karyotype indicative of one of the TS variations. Four of the sixteen individuals in variations lacked clinical stigmata associated with Turner Syndrome; the other nine had one or more of the usual clinical stigmata associated with TS. Two cases who had a complicated mosaic karyotype also possessed a Y chromosome. Three patients in group of classics had coarctation of the aorta and one patient in had variants of TS. 5 patients had primary hypothyroidism and received levothyroxine. two pts has hyperthyroidism. In Turner syndrome (TS), Growth Hormone (GH) treatment promotes growth and adult height. The benefit-risk ratio of supplementing GH with the weak androgen oxandrolone (Ox) is undefined. Growth hormone were administered (1.33 mg/kg/d) from the age of 8 years-old while estrogens were initiated at the age of 12 years-old. The increase in adult height (adult height minus predicted adult height) and safety factors were evaluated in a systematic manner.","PeriodicalId":436335,"journal":{"name":"Khalij-Libya Journal of Dental and Medical Research","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125964060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of Traditional Recipes for Oral Care by Local People in Janzour City, Libya: A Qualitative Study","authors":"","doi":"10.47705/kjdmr.216108","DOIUrl":"https://doi.org/10.47705/kjdmr.216108","url":null,"abstract":"Background and objectives: Traditional medicine (TM) has gained attention from the public globally in recent times. However, a lack of studies is identified for individuals’ use of traditional medicine to treat dental issues. Libya’s traditional medicine is an example of traditions that include a number of plant- and non-plant-based approaches to dental and oral health. A qualitative descriptive study was used to explore the views on, use of, and access to TM in dentistry as an alternative to conventional dental care and oral pain relief among Libyans. A purposive sample of residents older than eighteen living in the city of Janzour was used, and sixteen semi-structured individual interviews were conducted virtually. The interviews aimed to gather data, including locally used names for plants used medicinally for oral health, ways of using the treatments, their effectiveness, adverse impacts, and how far each remedy was in popular use. Data analysis was conducted, and the results were fairly consistent and indicated relatively high levels of knowledge and usage of TM. Three main themes were extracted from the interviews:1) Different perspectives of the participants on TM. 2) Current practice and experience with TM. 3) Reasons for choosing TM. This study provides understandings for dentistry professionals of the kinds of traditional medicines which the public use for oral health. Building such understandings and awareness of the use of traditional medicines can help dental professionals to promote discussions of the practices which patients use, providing an opportunity for education in this area.","PeriodicalId":436335,"journal":{"name":"Khalij-Libya Journal of Dental and Medical Research","volume":"5 8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131005732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surface Chemical Analysis of Hydroxyapatite Compared to Glass Ionomer and Amalgam Materials Used as Retrograde Filling Material in Dentistry","authors":"","doi":"10.47705/kjdmr.216107","DOIUrl":"https://doi.org/10.47705/kjdmr.216107","url":null,"abstract":"The aim of this study was to analyses the surface chemistry of different materials used as retrograde filling and compare it with bulk chemical analyses reported. Surface analysis carried out using an X—ray Photoelectron Spectrometer (XPS) built by VG Scientific. Samples of HAP, GIC and Amalgam materials used were prepared as Discs of 10 mm diameter. These transferred on to XPS aluminum stubs. XPS spectra were obtained from the wide scan spectrum of amalgam, the following elements are present: tin (23.2%); mercury (6.6%); carbon (19%) and oxygen (51.2%). The wide scan spectrum of GIC the elements present in the surface of the material are: oxygen (41.1%); aluminum (5%); silicon (4.6%); carbon (36.1%); calcium (11.3%); fluorine (2.1%) and sodium (0.T). The XPS spectra were obtained from the wide scan spectrum of Hydroxyapatite the elements present in the surface are: Oxygen (49-4%), Carbon (33-9%), calcium (8-6%), and Phosphorus (8-4%). In conclusion, the chemical XPS analyses of the materials used as retrograde filling, have shown them to vary considerably with respect to bulk chemical composition. Further surface analysis could include narrow scans, manipulation of surface chemistry, coupled with in vitro and in vivo tests and experiments to determine the importance of different surface components on biocompatibility.","PeriodicalId":436335,"journal":{"name":"Khalij-Libya Journal of Dental and Medical Research","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124650264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Nonsurgical Periodontal Therapy On IL-36 Levels in Serum, Gingival Crevicular Fluid of Type 2 Diabetic and Non-Diabetic Patients with Chronic Periodontitis","authors":"","doi":"10.47705/kjdmr.216106","DOIUrl":"https://doi.org/10.47705/kjdmr.216106","url":null,"abstract":"Objective: The aim of this study was to compare the level of inflammatory marker IL-36β in serum and gingival crevicular fluid in type 2 diabetic and non-diabetic patients with chronic periodontitis, before and after phase I therapy. Methods: A total of fifty subjects was included in this study; 20 Type 2 diabetic subjects with chronic periodontitis (group I) and 20 systemically healthy subjects with chronic periodontitis (group II) in addition to 10 systemically healthy subjects with clinically healthy gingiva as a control group (group III). The nonsurgical periodontal therapy was done to both group I and group II. Periodontal parameters, including plaque index, gingival index, bleeding on probing, probing depth and the clinical attachment level, in all the sites were recorded. GCF and serum were collected from all individuals included in the study; the first sample was collected from all groups (study and control) before phase I therapy. The second sample was collected 6 weeks after phase I therapy from group I& group II. Levels of IL-36β in GCF and serum were quantified using ELISA. Results. The current results showed statistically significant reduction in total level of IL-36β in serum and GCF in both groups; Type 2 diabetes with chronic periodontitis group (I) and systemically healthy with periodontitis group (II) after phase I therapy. Results also showed all clinical parameters were significantly improved after the phase I periodontal therapy in both groups I &II (p<0.001). Conclusions. Scaling and root planning (SRP) is the mainstay of treatment of periodontal diseases as SRP was effective in improving clinical parameters in diabetic and non-diabetic patients with chronic periodontitis. IL-36β could be used as a potential diagnostic marker for periodontal disease activity in both serum and gingival crevicular fluid.","PeriodicalId":436335,"journal":{"name":"Khalij-Libya Journal of Dental and Medical Research","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123810191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anatomical Causes of Infertility Diagnosed Using Imaging Tests in A Group of 100 Women","authors":"","doi":"10.47705/kjdmr.216105","DOIUrl":"https://doi.org/10.47705/kjdmr.216105","url":null,"abstract":"Background and aims. The demographics of infertility differ dramatically from one region to another. Therefore, details about the causes and distribution of infertility of different localities and regions are essential for any effective management strategy. This study aimed to evaluate the distribution of the causes of infertility in a women population attending a tertiary healthcare facility. Methods. A cross-sectional observational study conducted in a gynecology clinic on female patients who came for infertility consultation or treatment between June -2019 and December-2020. The cause of infertility was diagnosed by the history, examination and investigation. Data were arranged in tables, and the frequency of each factor was calculated and therefore the prevalence was estimated using SPSS 25. Results. secondary infertility (53%) was more prevalent than primary infertility (47%). Pelvic inflammatory disease was the leading cause (40%) and it was associated with tubal factor infertility. Second cause was polycystic ovarian syndrome (PCOS) (33%). Infertility causes changed as the age of marriage increased. In females married for less than 5 years, PCOs was the main cause of infertility. Conclusion. Causes of infertility in women change according to the age group and the age of marriage. This study shows that PID is the most common cause of infertility in females followed by PCO. A significant association between infections and tubal factor infertility was found.","PeriodicalId":436335,"journal":{"name":"Khalij-Libya Journal of Dental and Medical Research","volume":"381 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115097192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tooth Loss and Prosthesis Replacement at El-Koms City","authors":"","doi":"10.47705/kjdmr.216104","DOIUrl":"https://doi.org/10.47705/kjdmr.216104","url":null,"abstract":"Background and objective.Lossof at least one tooth disturbs the structural balance of the remaining natural teeth and may result in widening of proximal contacts, and other deformities.The proportion of full and partial edentulous people, as well as their prosthetic therapy, were investigated El-Koms cityMethods.Patient demographics, the percentage of partial and completely edentulous patients, and their prosthetic treatment were collected at four dental clinics. Health services. Ministry of Health in El-Koms city. The study was approved by the prosthodontics department, Faculty of Dentistry, University of Al Marqab.In 2020, a total of 1000 people were assessed in four dental clinics, with 472 individuals being chosen as part of the sample study group. Patients were chosen based on their age, which ranged from 25 to 75 years old, and their dental records. ResultsThe majority of the study participants (n = 472) had missing teeth as follows: 27 patients are completely edentulous, 36 patients having a single edentulous arch, 348 patients being partially edentulous, and 61patients having no missing teeth.Edentulous jaws werecorrelated statistically significantly with age, being more common in older age (𝑝value 0.03).Males had a higher percentage of missing teeth than females, and as people got older, edentulous persons grew increasingly common. In both jaws, Class IIIpartial edentulous people were the most common. Conclusion.There is a high demand for prosthodontic treatment. The incidence of edentulous patients in Libya, as well as predisposing factors, are both unknown","PeriodicalId":436335,"journal":{"name":"Khalij-Libya Journal of Dental and Medical Research","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127573463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultra-High-Molecular-Weight-Polyethylene (UHMWPE) as Desired Polymer Material for Biomedical","authors":"Assma Said","doi":"10.47705/kjdmr.216103","DOIUrl":"https://doi.org/10.47705/kjdmr.216103","url":null,"abstract":"It is very important that any materials used as implant material work in harmony with the body. There will be drawback with every material. No matter how good, as nothing can be 100% identical as the natural human tissue. The body operates in an environment at a constant temperature of 37°C and pH of 7.25, so choice of materials will have to withstand these conditions. Incorrect use of material can cause rejection by the body, infection and even cancer, leading to more pain and discomfort by the patient. In turn the possibility of even further damage to the joint. The implant must work in the same way as the body part it is replacing- clear understanding of how the joint works is needed. Ultrahigh molecular weight polyethylene is considered as the standard material for Artificial joints to decrease the total weight and the wear rate to make it more flexible. This is what makes Ultra-High-Molecular-Weight-Polyethylene (UHMWPE) such an appropriate polymer. It is very widely used in total hip and knee joint replacements having the highest known impact strength of any thermoplastic presently made, can highly withstand abrasion, and has a very low coefficient of friction. Therefore, these properties, connected with extremely low moisture absorption, make UHMWPE especial material for the medical industry due to good industrial impact and wear resistance sliding applications. For moving joints, the friction would be damaging without the natural lubrication. In implant components this does not exist, however UHMWPE is self-lubricating, making it ideal for component such as an acetabular cup, which would wrap around a metallic femoral head in a hip joint. Also, UHMWPE has high impact strength, high toughness, and low elastic modulus, but it has disadvantages such as low tensile, transverse and compressive strengths with high creep rate. This review article deals with the history of UHMWPE, its material properties that make it an ideal candidate for total joints, implant-component fabrication procedures and provides insights as to why some of the implants eventually fail.","PeriodicalId":436335,"journal":{"name":"Khalij-Libya Journal of Dental and Medical Research","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123876616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shaping Ability of Three Ni-Ti Rotary Systems in Curved Root Canals of Extracted Teeth","authors":"Showg Salem","doi":"10.47705/kjdmr.216102","DOIUrl":"https://doi.org/10.47705/kjdmr.216102","url":null,"abstract":"","PeriodicalId":436335,"journal":{"name":"Khalij-Libya Journal of Dental and Medical Research","volume":"257 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116017361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}