Animal Science and Food Technology最新文献

{"title":"The effect of carboxylates of food acids and substances of neurotropicmetabolic effect on biochemical indicators of blood and reproductive qualities of new-calved cows","authors":"Mykola Seba, Mansur Yahafarov, Ayaz Mammadov, D. Nosevych, Maryna Khomenko","doi":"10.31548/animal.3.2023.73","DOIUrl":"https://doi.org/10.31548/animal.3.2023.73","url":null,"abstract":"After calving, changes occur in the reproductive system of cows, aimed at restoring the ability to reproduce. These processes occur against the background of exhaustion from childbirth, lactation, and a negative energy balance, which often leads to violations of the reproductive function, so the search for methods that contribute to its restoration is urgent. The aim of the work was to investigate the effect of the drug with nerotropic-metabolic action as a stimulator of postpartum recovery in cows and improvement of fertility. The drug included L-arginine, sodium succinate, sodium glutamate and carboxylates of food acids (Fe, Ca, Mn, Cu, Zn). The study was conducted by the method of groups on cows of the Holstein breed. A control and two experimental groups of 15 heads each were formed. Cows of experimental group 1 were given injections in the subcaudal fold on the 5th, 7th and 9th day after calving, dividing the active substance into 3 parts. Cows of experimental group 2 were administered the drug intraparenterally in the first 1.5-2 hours after calving in a full dose once 21st days after calving, the cows of the experimental groups did not show any deviations in blood biochemical parameters compared to the reference values. But there was a tendency to decrease the amount of calcium, phosphorus, protein and carotene, compared to cows of the control group, which indicates the presence of an influence on metabolic processes. The content of total protein in the blood of cows of experimental group 2 was close to the lower limit of the physiological norm. No placental retention was observed in the cows of the experimental groups, while in the control group this disorder was present in 33% of cows. The introduction of the drug made it possible to reduce the frequency of endometritis by 20%, to shorten the period until the beginning of the first oestrus by 7th and 10th days, to increase fertility after the first insemination by 6 and 13 percentage points, and to shorten the service period by 16th and 22nd days. The second experimental group obtained a higher efficiency of using the drug. It is advisable to use the results of research on dairy farms. Intraparenteral administration of the drug 1.5-2 hours after calving will improve the process of restoring the sexual function of cows and their fertility","PeriodicalId":423813,"journal":{"name":"Animal Science and Food Technology","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139354369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increasing the productivity of young pigs in the context of overcoming technological stress","authors":"Marina Koroban, V. Lykhach, Anna Lykhach, Y. Barkar, Sergiy Chernysh","doi":"10.31548/animal.3.2023.47","DOIUrl":"https://doi.org/10.31548/animal.3.2023.47","url":null,"abstract":"Production activities are mandatory elements of the technological cycle and cause various types of animal stress. The use of feed additives based on plant extracts in a liquid composition for pigs is an important strategy for developing their stress resistance and increasing productivity. The purpose of the experiment is to investigate the optimal duration of drinking a liquid feed additive in different periods of technological stress on pig productivity parameters. In farm conditions, 90 crossbred pigs were randomly divided into three groups according to generally accepted zootechnical methods. Animals of the I group (control) were fattened according to the basic technology; animals of II experimental group – BT+ administration of liquid feed additive three days before and three days after the technological action, and pigs of III experimental group – BT+ administration of liquid feed additive seven days before and seven days after. Swine of the III research group at the age of 11-26 weeks probably exceeded their counterparts in live weight by 1.70-5.43 kg, in average daily gains in the period from 14-22 weeks – by 20.0-82.5 g. According to the precocity indicator, pigs of the III group reached a live weight of 100 kg 6.1 days earlier and 120 kg 7.3 days earlier. The values of average daily gains in pigs of the III group were probably higher by 51.9 g – for a live weight of 100 kg and 38.1 g – for 120 kg. Pigs of III group had the lowest level of feed conversion – 0.11 kg at 100 kg and 0.14 kg at 120 kg. It has been established that in order to increase the productivity of pigs, it is worth drinking LFA 7 days before and 7 days after the period of technological stress during the entire growing period. The practical significance of the experiment is to identify the optimal duration of the use of liquid feed additive to increase the stress resistance and productivity of pigs","PeriodicalId":423813,"journal":{"name":"Animal Science and Food Technology","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139355623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of beekeeping products in the technology of raw smoked sausages","authors":"O. Shtonda, Viktoriia Kulyk, Kateryna Semeniuk","doi":"10.31548/animal.3.2023.86","DOIUrl":"https://doi.org/10.31548/animal.3.2023.86","url":null,"abstract":"The safety of fermented sausage products is due to the elimination of a number of risks of biological origin, mainly of a microbiological nature. One of the most promising directions for the development of barrier technologies is the use of starter cultures and the biologically active substances produced by them in the production of fermented meat products. The research aims to study the influence of starter cultures and honey on a complex of physico-chemical, structuralmechanical, organoleptic indicators and nutritional value of raw smoked sausages. The article presents changes in the physical and chemical parameters of raw smoked sausages when using starter cultures in combination with different types of honey. When performing the work, 4 types of honey were used: from sunflower, phacelia, honeydew honey and from medicinal herbs. At the first stage of research, the proportion of honey application and the type of starter crops were selected. The next stage of the study was to determine the impact of the starter cultures included in the product BIOSTART PLUS 50 (lactic acid bacteria Lactobacillus Sake and yeasts Debaryomyces Hansenii) and honey on moisture loss during fermentation of raw smoked sausages, physicochemical parameters of finished products. Determination of moisture content in samples was carried out by drying in a drying cabinet at a temperature of 103±2°C. Conducted studies prove that the use of the product BIOSTART PLUS 50 (lactic acid bacteria Lactobacillus Sakei and yeasts Debaryomyces Hansenii) and honey allows shortening the ripening and drying technological process. A comparative analysis of manufactured raw-smoked sausages in terms of nutritional value makes it possible to state that manufactured raw-smoked sausages with the addition of honey meet the requirements of the standard. The results of the set of studies carried out in this work showed that the use of a composition of lactic acid bacteria and yeast with the use of honey as a source of carbohydrates will allow intensifying the technological process and improve the quality of finished products","PeriodicalId":423813,"journal":{"name":"Animal Science and Food Technology","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139358474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteolytic processes in cheese analogues ripening","authors":"Danylenko Danylenko, L. Bal-Prylypko, Taisia Ryzhkova, Sergii Verbytskyi, Oleksandr Panasiuk","doi":"10.31548/animal.3.2023.20","DOIUrl":"https://doi.org/10.31548/animal.3.2023.20","url":null,"abstract":"The research relevance is predefined by the theoretic basics of their production processes not being sufficiently studied, including the proteolysis despite the wide production of cheese analogues incorporating vegetable substances. The research aims to determine the effect of the content of cheese analogues, namely milk fat substitutes, soy protein isolates, and lactobacilli species Lactococcus lactis ssp. lactis and L. lactis ssp. cremoris, L. lactis ssp lactis bv. diacetilactis as well as Leuconostoc mesenteroides by the physical, chemical, and sensorial traits, and by amino acids content of cheese analogues. Determination of the physical and chemical parameters was fulfilled according to the standards: active acidity (рН) – DSTU 8550:2015; solids content (by weight) – DSTU 8552:2015 and ISO 5534:2004; non-casein nitrogen content (by weight) – ISO 17997-1:2004; total protein (by weight) – ISO 8968-1:2014 and ISO 8968-5:2001; casein content (by weight) – ISO 17997-1:2004. The fractional composition of proteins was determined using the Polyacrylamide Gel Electrophoresis. While in the cheese dough after analysing the differences in the spectrum of free amino acids between the variants were insignificant, in ripe cheese analogues the accumulation was significantly less than in the control cheeses, only one ripe cheese analogue showed the total amount of free amino acids and was almost equal to the control. During ripening, the content of these compounds increased in cheese analogues from 2.2 times to 3.3 times, in the control the content of free amino acids increased by 2.5 times, and individual free amino acids accumulated from 3-7 to 30-40 times. Active proteolytic processes led to the appearance of all ripe products of such essential amino acids as methionine and isoleucine, there was also an increase in the level of glycine. The practical significance of the study is determined by substantiating rationale proteolytic processes in the manufacture of cheese analogues to ensure consumer quality close to the quality of cheeses made of milk","PeriodicalId":423813,"journal":{"name":"Animal Science and Food Technology","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139358612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological variability of individual colour forms of Cyprinus carpio haematopterus of different origin","authors":"Oleksandr Lysak, Petro Shevchenko, Mykola Ratushny, Dmytro Khrystenko, Ganna Kotovska","doi":"10.31548/animal.3.2023.61","DOIUrl":"https://doi.org/10.31548/animal.3.2023.61","url":null,"abstract":"The relevance of the work on the study of morphological indicators of koi lies in the increased attention at international koi exhibitions to such external indicators as colour, shape, and body proportions of fish that occupy high prize places and receive monetary rewards of hundreds of thousands of US dollars. The purpose of the work was to study the morphological features of various coloured forms of various origins. Such indicators are not taken into account in traditional fish breeding, which also adds novelty to the conducted research. 280 koi samples were selected for analysis. The work evaluated the variability of plastic features of fish of individual colour forms (magoi, kumonryu, utsurimono, kohaku, kawarimono, bekko, doitsu bekko, doitsu kawarimono) of different origins (Japanese, Turkish, Israeli, and Ukrainian). As a result of the work, it was established that there was not found a statistically significant difference between different colour forms of the same origin. At the same time, a reliable difference was established in fish of Israeli origin in terms of features characterizing the shape and proportions of the head in relation to the proportions of the body. The distribution of morphological indices of the studied individuals of different samples in the space of the main components also confirms the reliable differences of the Israeli morphotype from fish of Japanese, Turkish, and Ukrainian origin according to the characteristics related to the ratios of the head parts and body proportions. The obtained data is the basis for the work of breeders and breeders of koi carp who wish to grow valuable specimens – potential winners of international koi carp competitions","PeriodicalId":423813,"journal":{"name":"Animal Science and Food Technology","volume":"75 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139359385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of the market of meat products in Ukraine","authors":"A. Antoniv, L. Adamchuk, E. Ivanišová, R. Chlebo, E. Topal","doi":"10.31548/animal.2.2023.9","DOIUrl":"https://doi.org/10.31548/animal.2.2023.9","url":null,"abstract":"Meat and meat products are among the main products of the human diet, which contain the essential nutrients, biological, macro and microelements that are important for the full functioning of the human body. Due to the significant environmental impact associated with industrial meat production, combined with the ever-growing world population, it is important to investigate the current state and production of the meat industry. The purpose of the study was to determine the volumes and possibilities of production of certain types of meat products in Ukraine, their market share, prospects for production in the future, and the ability to provide food industry enterprises with raw materials for the production of new food products. The work was carried out by analysing scientific, regulatory and technical literature, statistical evaluation of data, and comparing the results obtained with the findings of other scientists. The analysis of the industry indicates the decline of certain areas in animal husbandry, in particular, cattle breeding and the production of meat, meat products from beef and veal; a decrease in the number of farm animals, namely by 23% of bovine cattle, 6.9% of pigs, and 2.4% of poultry. During the analysed period, poultry meat production increased by 17%, while the number of business entities increased by 25 %. At the same time, beef and veal production decreased by 27.8%, while pork production increased by 33%. Based on the results obtained, it is possible to continuously provide processing enterprises with meat raw materials, namely, chicken meat for the production of meat products, and it is also relevant to introduce resource-saving technologies for obtaining a product with less weight loss and waste during processing. The study results can be used by enterprises when planning the release of products to the market, developing new recipes, improving existing production technologies, and understanding trends and volumes of meat and meat products on the Ukrainian market","PeriodicalId":423813,"journal":{"name":"Animal Science and Food Technology","volume":"111 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131873900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological and biochemical aspects of the carp organism in conditions of increasing their viability when stocking water bodies","authors":"O. Honcharova, V. Bekh, B. Glamuzina","doi":"10.31548/animal.2.2023.28","DOIUrl":"https://doi.org/10.31548/animal.2.2023.28","url":null,"abstract":"The relevance of the study is conditioned by the defining role of the stage of early development in the ontogenesis of young fish in the formation of their growth rate potential. The physiological and biochemical status of the body determines the adaptive capabilities of hydrobionts, so the use of biologically active substances when rearing fish was chosen as one of the vectors for improving the development parameters. The purpose of the study was to determine the effectiveness of introducing technological elements (supplementary feeding, rearing) in the early stages of the ontogenesis of young fish. The study was based on theoretical (analysis, synthesis, comparison, modelling), experimental, and laboratory methods adopted in fisheries, physicochemical studies. A clinical examination of fish, monitoring of growth rates, weight gain, survival rate, and physiological state was carried out. The results show an increase in the viability of young fish, activation of metabolic processes, and improvement of blood morphological and functional parameters. The average weight of fingerlings exceeded the parameters in the control group for carp by 3.5% and for silver carp by 3.8%. The total number of red blood cells in the silver carp experimental group was 7.4% higher than the control (p < 0.05), haemoglobin content – by 4.7%, haematocrit – 9.9%. The total number of red blood cells in the blood of carp in the experimental group was 4.9% higher, and the haemoglobin content was 3.1% higher than in the control group. In the experimental group, the total protein content exceeded the value in relation to the control group for silver carp by 15.9% (p < 0.05), and for carp – by 23.9% (p < 0.01). Enzymatic activity in the experimental group was higher compared to the control group. The proposed method of feeding helps increase the growth rate of fish. The practical value of the study is to help improve qualitative and quantitative parameters, in particular, the biochemical composition of the muscle part for carp and silver carp in samples in the experimental group in comparison with control values","PeriodicalId":423813,"journal":{"name":"Animal Science and Food Technology","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115525980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective properties of the fleece of Taurian ewesof the Askanian fine fleece breed depending on the breeding differentiation rank","authors":"Vasyl Tyrunskiy, N. Bogdanova, Peter Lyutskanov","doi":"10.31548/animal.2.2023.76","DOIUrl":"https://doi.org/10.31548/animal.2.2023.76","url":null,"abstract":"The study of factors affecting the humidity and contamination of the outer and inner zones of the staple, which further affects the yield of pure wool and its technological properties, is one of the current problems of fine-fleeced sheep breeding. Purpose of the study – evaluate the protective properties of wool fleece depending on the rank of breeding differentiation of sheep. The determination of ranks is based on the regularities of the normal distribution of animals in the population and herd reproduction indicators. 10 ranks were used. The paper presents the results of a laboratory study of the content of mineral impurities and the degree of contamination of the staple. Sheep were divided into 10 ranks of breeding differentiation. The regularities of the normal distribution of animals in the population are considered. The obtained data indicate that the variety of sheep in terms of the content of mineral impurities in wool is very large. The coefficient of variation is 35% with a variation in the ranks of breeding differentiation from 13.7 to 38.4%. With an increase in the group of the total value of sheep for breeding purposes, the value of the coefficient of variation decreases (rs=-0.871±0.245). With an increase in the ranks of breeding differentiation, the average absolute indicators of contamination of the outer zone of the staple tend to increase (rs = +0.257 ± 0.483), and the relative values decrease (rs =-0.600 ± 0.400). The average absolute contamination rate of the inner zone of the staple is 3.02 cm with a range of ranks from 2.83 to 3.29 cm. According to the relative degree of contamination, these indicators are 32.5% and from 31.0 to 35.2%, respectively. With an increase in the breeding differentiation rank of sheep, the absolute indicators of contamination of the length of the inner zone of the staple increase (rs = +0.600 ± 0.400), and relative values tend to decrease (rs =-0.486 ± 0.437). The individual variety of ewes in terms of the degree of contamination of the inner zone of the staple is quite high. The coefficients of variation for absolute and relative indicators of this feature are 28.5 and 30.4%, respectively. The value of the coefficients of variation decreases slightly with an increase in the ranks of breeding purposes of animals. The rank correlation is -0.371 ± 0.464 in absolute terms and -0.543 ± 0.420 in relative terms. Breeding differentials of the leading groups of ewes are low. Using this rank technique allows controlling the system of sheep valuation, supplementing it with the value of breeding differentials at the final stages of analysis and selection; in production conditions allows forming technological groups of sheep to produce outstanding ram breeders","PeriodicalId":423813,"journal":{"name":"Animal Science and Food Technology","volume":"126 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116791683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of the raw material base for pectin production","authors":"Nataliia Mushtruk, M. Mushtruk","doi":"10.31548/animal.2.2023.57","DOIUrl":"https://doi.org/10.31548/animal.2.2023.57","url":null,"abstract":"The addition of pectin to food products is important because this natural polysaccharide plays a key role in improving the quality and stability of the product, it allows for high-quality preservation of products that have a long shelf life, which is important for the health and wellbeing of consumers. The purpose of the study is to investigate the main raw materials of plant origin for the synthesis of pectin, which can be used as a promising raw material for food industry enterprises in Ukraine. The study was carried out using various methods of analysis, a thorough comparative analysis of papers by Ukrainian and foreign researchers, which are published in the scientometric databases Scopus, Web of Science, Journal Citation Reports, Scimago Journal & Country Rank, and Google Scholar. Based on the results of analytical studies, the main raw materials used for the production of pectin and pectin-containing pastes were analysed, and the main problems of the production process were identified. It has been found that adding pectin to food products can have a significant number of benefits: increasing the viscosity of the product, which is useful for creating a thick texture of the product; increasing the resistance of the product to heat treatment, reducing the risk of product separation or quality degradation; increasing functionality; introducing it into products can help improve the consistency and appearance of the product, add taste and color properties, and reduce the amount of sugar that must be added to the product to ensure viscosity and taste. It is established that the main raw material for the production of pectin in Ukraine is apple fruits. It is proven that pectin can be obtained from other fruits, berries, and vegetables, such as gooseberries, currants, cherries, pumpkins, etc. The intensification of the pectin production process should provide the population with a high-quality product that will prevent various types of intoxication by removing radionuclides from the body and contribute to the recovery of people in the post-war period. The practical significance of this study lies in the ability to help manufacturers find optimal sources of raw materials with a high pectin content to ensure the quality of the final product. In addition, this study will also facilitate the development of appropriate technological solutions for the optimal process of producing pectin from the selected raw materials","PeriodicalId":423813,"journal":{"name":"Animal Science and Food Technology","volume":"60 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126153585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of genotyping features of bovine cattle individuals at the CSN2 locus using ACRS-PCR methods","authors":"R. Kulibaba, M. Sakhatskyi, Y. Liashenko","doi":"10.31548/animal.2.2023.44","DOIUrl":"https://doi.org/10.31548/animal.2.2023.44","url":null,"abstract":"In the context of solving the problem of obtaining high quality dairy products from livestock, the issue of determining the type of beta-casein (A1 and A2) in the protein fraction of milk becomes essential. Purpose – to analyse the use of ACRS-PCR methods for differentiation of A1 and A2 alleles of bovine beta-casein locus. Genotyping features were analysed using the artificially created restriction site polymerase chain reaction method utilising TaqI and DdeI restriction endonucleases. The electrophoretic distribution of DNA fragments in agarose gels of various concentrations was used to analyse restriction patterns. Based on the results of bioinformatic analysis of the nucleotide reference sequences of the experimental fragment of the beta-casein gene, it was found that the primer system for the ACRS-PCR DdeI method is characterised by higher parameters of flanking efficiency of the target DNA site compared to the ACRS-PCR TaqI system due to significantly greater effectiveness of hybridisation of oligonucleotides on the target DNA. Based on the results of laboratory tests of both methods, it is proposed to use an additional procedure for analysing the fluorescence intensity of individual elements of restriction patterns, which allows reducing the number of false genotyping that occurs in both cases (based on the results of using both methods) due to the appearance of non-specific amplification/restriction fragments within the size of target restrictions. The application of the ACRS-PCR DdeI method provides more differentiated patterns of the corresponding genotypes in agarose gel compared to the ACRS-PCR TaqI method, but leads to higher material costs for conducting research. These disadvantages of using primer systems for ACRS-PCR of the beta-casein locus determine the relevance of developing alternative methods for typing A1 and A2 alleles which include allele-specific PCR. The use of results is promising for solving the problems of genotyping cattle individuals of different breeds by A1 and A2 alleles of the beta-casein locus","PeriodicalId":423813,"journal":{"name":"Animal Science and Food Technology","volume":"77 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123855106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}