International Journal of Medical Laboratory Research最新文献

{"title":"GIANT RETROPERITONEAL LIPOMA : A CASE REPORT OF A RARE ENTITY","authors":"S. Kundal, Ds Chauhan, S. Singh","doi":"10.35503/ijmlr.2021.6208","DOIUrl":"https://doi.org/10.35503/ijmlr.2021.6208","url":null,"abstract":": Lipomas are the most common soft tissue tumors in adult. They commonly arise in extremities. Retroperitoneal lipoma is an extremely rare benign mesenchymal neoplasm. Clinically, patients present with lump in the abdomen along with pressure symptoms such as pain abdomen. Herein, we report a rare case of giant retroperitoneal lipoma in a 25 year old young female, who presented with lump and pain abdomen since 3 months.","PeriodicalId":420042,"journal":{"name":"International Journal of Medical Laboratory Research","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129025485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PERFORMANCE EVALUATION OF BIOLOGICAL REFERENCE PREPARATIONS (BRP) HUMAN ALBUMIN AND HUMAN IMMUNOGLOBULIN FOR ELECTROPHORESIS (PROTEIN COMPOSITION) AFTER RECOMMENDED PERIOD OF ITS USE IN TESTING LABORATORIES","authors":"AA Talwar, Y. Madhu, Girija LV, Varun Singh, Varsha Singh, M Kumari","doi":"10.35503/ijmlr.2023.8206","DOIUrl":"https://doi.org/10.35503/ijmlr.2023.8206","url":null,"abstract":"","PeriodicalId":420042,"journal":{"name":"International Journal of Medical Laboratory Research","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130284747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"COBALT TOXICITY/POISONING WITH ANALYTICAL ASPECTS AND ITS MANAGEMENT","authors":"A. Jaiswal, R. Kumar, Aishwarya Thakur, P. Puri, R. Kumar, R. Kanojia","doi":"10.35503/ijmlr.2019.4305","DOIUrl":"https://doi.org/10.35503/ijmlr.2019.4305","url":null,"abstract":"Cobalt is one of the essential Components found in trace amounts in human beings, has an atomic number of 27, molecular mass of 58.93 units and represented by the symbol Co. It is important for the formation of Cyanocobalamin or Vitamin B12. Normally a healthy diet contains 5 to 50g of cobalt in a day. Cobalt generally does not cause any toxicity or poisoning until the exposed to large amounts and for longer time period. Poisoning can be caused by three ways which includeSwallowing too much, inhaled too much in lungs and constant contact with the skin. Various effects are visible in case of cobalt poisoning which includes cardiovascular effects, neurological, haematological, immunological, dermatological, ophthalmological, gastrointestinal and reproductive system dysfunction. The biological samples which are analysed for cobalt poisoning includes urine, blood, serum and tissues. The various aspects along with the treatment and hospital management is discussed in this paper. Other than that, the analytical techniques which include HR-ICP-MS, XRF, UV Spectrophotometry, ICP-OES are also discussed which are used in the diagnosis.","PeriodicalId":420042,"journal":{"name":"International Journal of Medical Laboratory Research","volume":"62 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125505587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IMPROVEMENT OF “LACTULOSE-MANNITOL RECOVERY RATIO TEST” OF INTESTINAL ABSORPTION BY LC-MSMS","authors":"Raffaele Conte, A. Cavallo, Antonio Fico","doi":"10.35503/ijmlr.2019.4201","DOIUrl":"https://doi.org/10.35503/ijmlr.2019.4201","url":null,"abstract":"Liquid chromatography-tandem mass spectrometry (LC-MS/MS) has seen enormous growth in clinical laboratories during the last decades due to the fact that it offers analytical specificity superior to that of immunoassays or conventional high performance liquid chromatography. Then, most chemicals and metabolites are now assayed by LC-MS/MS. The “LactuloseMannitol recovery ratio (LAMA test)” is a non-invasive gastrointestinal test for the assessment of intestinal absorption and barrier function of the bowel. This article describes an optimized LC-MS/MS method used for the determination of these two sugars, characterized by rapidity, sensitivity, and easy automation.","PeriodicalId":420042,"journal":{"name":"International Journal of Medical Laboratory Research","volume":"124 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128151943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AN ASSESSMENT OF AUTOIMMUNITY IN ARTHRITIS PATIENTS","authors":"O. Odewusi, M. Abdulmumin, O. Olaniyan","doi":"10.35503/ijmlr.2022.7108","DOIUrl":"https://doi.org/10.35503/ijmlr.2022.7108","url":null,"abstract":"Objectives: The goal of this study is to estimate autoimmune biomarkers that characterize the development and severity of arthritis, but probably normalize following successful therapy. Materials and methods: In this study a total of 109 subjects were used out of which treated and untreated arthritics were 48 and 44 respectively, the remaining 17 were healthy individuals which were used as control. Samples were collected from patients attending Rheumatology and Orthopedic clinic of Federal Teaching Hospital Ido-ekiti, Ekiti State Nigeria. Antinuclear antibody was estimated using Enzyme Linked Immunosorbent Assay (ELISA) while Lupus Erythematosus cells were ascertained microscopically using Leishman staining technique. All parameters were assessed in treated and untreated arthritic patients relative to healthy subjects. Body mass index was also calculated. Statistical analysis was done using SPSS. Results: Body mass index and Antinuclear antibodies were significantly higher in treated and untreated arthritics compared to control (P<0.05). When treated and untreated arthritics were compared, Body mass index and Antinuclear antibody were found to be significantly higher in untreated arthritics (P<0.05). Antinuclear antibody and Age correlated directly in untreated arthritics. Lupus Erythematosus cell prevalence was found to be higher in untreated arthritics having a percentage Lupus Erythematosus test positivity of 6.8% compared to the 2.1% seen in treated arthritics. Conclusion: It was found that Autoimmunity in arthritics can be significantly lowered through treatment with Arthritic drugs, diets, life style modifications over a period of time. The study suggests that Antinuclear antibody and Lupus Erythematosus estimations could be adopted as markers of diagnosis, prognosis and monitoring of arthritis.","PeriodicalId":420042,"journal":{"name":"International Journal of Medical Laboratory Research","volume":"5 14","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133169467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EVALUATION OF SELECTED RENAL MARKERS IN HYPERTENSIVE SUBJECTS IN EKITI STATE, NIGERIA","authors":"A. Oluboyo","doi":"10.35503/ijmlr.2020.5202","DOIUrl":"https://doi.org/10.35503/ijmlr.2020.5202","url":null,"abstract":"Hypertension has become a serious health challenge in the 21 st century because it may cause organ damage as it progresses and could result in increased morbidity and mortality. The study evaluated selected renal markers (urea, creatinine, uric acid and cystatin C) in hypertensive subjects relative to control. A total of 92 subjects were investigated consisting of 31 hypertensive subjects on treatment, 31 hypertensives not on treatment and 30 apparently healthy subjects who served as control subjects. The levels of urea, creatinine and uric acid were determined using spectrophotometric methods while cystatin C was analyzed using enzyme linked immunoassay (ELISA) technique. Blood pressure and body mass index (BMI) were also measured in addition to the selected markers. The results showed that urea, uric acid, blood pressure and BMI were significantly higher at p<0.05 while creatinine and cystatin C did not show significant difference (p>0.05) in hypertensive subjects compared with control. It was concluded that urea, uric acid, blood pressure and BMI were significantly high in hypertension. Measurement of these markers could be helpful in identification of patients at high risk of developing renal complications associated with hypertension.","PeriodicalId":420042,"journal":{"name":"International Journal of Medical Laboratory Research","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133996059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CAUSES OF NEUTROPENIA AND BACTERIAL INFECTIONS: A RETROSPECTIVE STUDY","authors":"E. Manuli, J. Intra, G. Limonta, P. Brambilla","doi":"10.35503/ijmlr.2020.5102","DOIUrl":"https://doi.org/10.35503/ijmlr.2020.5102","url":null,"abstract":": Neutropenia is characterized by an absolute neutrophil count less than 1,500 cells/µL and an increased risk of infection. Retrospective data of 267 inpatients (cases) with neutropenia and 333 inpatients without neutropenia (normal cases) were consecutively collected from laboratory database of the Italian Hospital of Desio. Subjects with neutropenia caused by chemo- or radiotherapy treatment, myelodysplastic syndromes, chronic liver diseases, and drug-induced had significantly higher rates of infection than normal cases ( p < 0.01). Patients whose neutropenia was caused by autoimmune or idiopathic diseases showed no significant differences ( p > 0.4). Subjects with neutropenia caused by myelodysplastic syndromes or chemo- or radiotherapy treatment had an increased risk of infections from mild and moderate to severe neutropenia ( p trend < 0.0001). Patients affected by myelodysplastic syndromes had a significant shift from urinary to respiratory tract ( p = 0.008) and to systemic infections with positive blood cultures (SI+PBC) ( p = 0.02) compared to normal cases. Subjects with recent chemo-or radiotherapy treatment presented a significant shift only to SI+PBC ( p = 0.01). Collectively, it is important to pay more attention to specific causes of neutropenia and the degree of neutrophils count, which are associated with different risks and sites of the infections.","PeriodicalId":420042,"journal":{"name":"International Journal of Medical Laboratory Research","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133178302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PATHOGENIC BACTERIAL ISOLATES FROM DIABETIC FOOT INFECTIONS PATIENTS AND THEIR SUSCEPTIBILITY TO ANTIBIOTICS IN SELECTED PUBLIC HOSPITALS, SANA’A, YEMEN","authors":"Nabilah Shiaf, Al-Samet Ebrahim M, Jameel Nosibah L","doi":"10.35503/ijmlr.2023.8204","DOIUrl":"https://doi.org/10.35503/ijmlr.2023.8204","url":null,"abstract":"Sickle cell disease (SCD) is a group of blood genetic disorders resulting in an abnormality in the Diabetic foot infections remain a major public health problem and cause socioeconomic burdens to affected people. Clinically infected foot ulcers require treatment guided by appropriate cultures and antimicrobial susceptibility testing. This study aimed to assess the bacterial profile and antimicrobial susceptibility pattern of isolates from diabetic foot infections in selected public hospitals, Sana'a, Yemen. A cross-sectional study was conducted from June 2021 to July 2022 at Al-Gomhori teaching hospital, Kuwait teaching hospital, and AlThawrah public hospital in Sana'a, the capital city of Yemen. The study included 135 adult patients with infected diabetic foot ulcers. Convenient sampling was employed. Wound aspirates from the foot ulcers were collected aseptically and inoculated into Blood, MacConkey, Chocolate and Mannitol salt Agar. The antimicrobial susceptibility patterns were conducted by disk diffusion method. The data was analyzed with SPSS v.20 for windows. The results revealed that; One hundred ninety bacterial isolates were identified among 135 patients. Among them, 62.96% had mono-bacterial infection while 37.04% had mixed bacterial infections. Gram negative aerobic bacterial infections were more accounting cases 63.7% than, Gram positive aerobic bacteria 36.3%. The most commonly isolated bacteria were S. aureus 26.3%, followed by Klebsiella spp 22.1% and Proteus spp 11.1%. In general, 73.68% of the isolates developed multidrug resistance to at least one drug in three different classes of antibiotics. Meropinem and amikacin appeared to be the best antibiotics for therapy against Gram negative and cefoxitin and vancomycin against Gram positive organisms.","PeriodicalId":420042,"journal":{"name":"International Journal of Medical Laboratory Research","volume":"36 8","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114135890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MORPHOPATHOLOGY OF PANCREAS IN DIABETES MELLITUS- A PROSPECTIVE STUDY DONE IN 50 AUTOPSY CASES","authors":"S. Rani, VK Bodal, A. Aggarwal, Babita Rani","doi":"10.35503/ijmlr.2019.4302","DOIUrl":"https://doi.org/10.35503/ijmlr.2019.4302","url":null,"abstract":"The pancreas comprises exocrine and endocrine compartments, with the latter comprising the pancreatic islet with its endocrine cells. In both type1 and type2 diabetes, several morphological changes occur in the pancreas, but the predominant feature of beta cell loss. Our aim of this study is to find out of histopathological changes in pancreas due to diabetes and correlate it with clinical findings. Methods: A prospective study was done on 50 autopsy specimens of pancreas. Specimens were fixed in formalin and tissue was adequately processed. The sections were stained with routine haematoxylene and eosin stain. Results: In our study 86% cases were of T1DM and 14% of T2DM. Most common age group was between 40-60 years (62% cases). 68% cases had duration of DM of 10-20 years. On histopathological examination, 85% Cases of T1DM revealed cytolysis and inflammation in exocrine pancreas. In cases of T2DM cytolysis was seen in 32% cases, inflammation in 25% and fibrosis in 16% cases.. Fibrosis was present in 14% cases of T1DM and 25% cases of T2DM in endocrine pancreas. Insulitis in endocrine pancreas was seen in 85% cases of T1DM and 34% cases of T2DM. Thus, the most common finding was reduced beta cell mass in T2DM and insulitis in T1DM. Findings were correlated with age, sex and duration of diabetes. Conclusion: With increasing incidence of diabetes knowledge of pathogenesis of changes due to diabetes in pancreas may lead to research of further targeted therapy in better management of diabetes in future. Changes that occur in pancreas due to diabetes are more evident in long standing diabetes and early changes are not evident in routine histopathological sections.","PeriodicalId":420042,"journal":{"name":"International Journal of Medical Laboratory Research","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123115370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AN ASSESSMENT OF ANTINUCLEAR ANTIBODIES AND LE CELL POSITIVITY IN ESSENTIAL HYPERTENSIVE SUBJECTS","authors":"Odewusi Odeyinka Olufunsho, Ebitimi Cocodia, Olaniyan Olayinka Olaolu","doi":"10.35503/ijmlr.2021.6305","DOIUrl":"https://doi.org/10.35503/ijmlr.2021.6305","url":null,"abstract":"Objectives: This study is designed to assess and ascertain the serum level of antinuclear antibodies (ANA) concentration and lupus erythematosus (LE) positivity in treated and untreated hypertensive patients relative to non-hyprtensive apparently healthy individuals. Methods: This study investigated a total number of 116 subjects age between 18-70 years comprising 54 hypertensives on known anti HBP medications, 35 hypertensives not on anti HBP medications and 27 apparently healthy individuals. Blood samples were collected from patients attending the General Out-patient Department of Federal Teaching Hospital Ido-ekiti, Ekiti State Nigeria. Blood pressure was taken from the non-dominant amusing appropriate cuff size and mercury sphygmomanometer, while, blood sample was Anti-nuclear antibodies was measured using ELISA while LE cells was ascertained with Leishman-stained microscopy of defibrinated blood. Statistical analysis was done using SPSS, all values were significant at p<0.05. Results: Both systolic and diastolic blood pressure were observed to be significantly higher in both treated and untreated essential hypertensives compared with controls (P<0.0001). Serum ANA levels were also found to be significantly higher in untreated hypertensives compared with both treated hypertensives and controls (P<0.0001). Diastolic blood pressure correlated directly with systolic blood pressure and ANA levels in untreated hypertensives (r=0.556) (r=0.318) respectively but inversely with BMI in treated hypertensives. LE cell positivity was found to be higher in untreated hypertensives having a percentage LE test positivity of 2.9% as compared to the percentage in treated hypertensives where 1.9% were positive. Conclusion: It was deduced that serum elevated ANA levels as well as LE positivity are also characteristics of essential hypertension. It was also found out that the possibility of LE positivity and ANA levels in essential hypertensives increases with weight, age and being of the female gender. KEYWORD: Essential hypertension; Antinuclear antibodies, LE cell. RESEARCH ARTICLE Corresponding Author: ODEWUSI, Odeyinka Olufunsho Department of Medical Laboratory Science, Afe-Bablola University, Ado-Ekiti, Ekiti State ISSN No. 2456-4400 Int J Med Lab Res 2021, 6(3):35-43 International Journal of Medical Laboratory Research (Vol. 6 Issue 3, December 2021) www.ijmlr.com/IJMLR© All rights are reserved","PeriodicalId":420042,"journal":{"name":"International Journal of Medical Laboratory Research","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121756643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}