Iranian Journal of Pharmaceutical Research : IJPR最新文献

The Impact of Resveratrol Supplementation on Inflammation Induced by Acute Exercise in Rats: Il6 Responses to Exercise 补充白藜芦醇对大鼠急性运动引起的炎症的影响:il - 6对运动的反应

Iranian Journal of Pharmaceutical Research : IJPR Pub Date : 2019-05-01 DOI: 10.22037/ijpr.2019.1100684

R. Vafaee, H. Hatamabadi, H. Soori, M. Hedayati

{"title":"The Impact of Resveratrol Supplementation on Inflammation Induced by Acute Exercise in Rats: Il6 Responses to Exercise","authors":"R. Vafaee, H. Hatamabadi, H. Soori, M. Hedayati","doi":"10.22037/ijpr.2019.1100684","DOIUrl":"https://doi.org/10.22037/ijpr.2019.1100684","url":null,"abstract":"Severe physical activity leads to a sharp increase in free radicals, an oxidative stress, inflammation, and tissue damage. Resveratrol as one of the antioxidants can be effective in preventing the effects of oxidative stress. Therefore, the present study was aimed to evaluate the effect of trans-resveratrol supplementation and training exercise on inflammation-related factors. Sixty-four male Wistar rats were divided into six groups, each group consisting of 16 animals: 1) excursive + trans-resveratrol, 2) exercise group, 3) trans-resveratrol group, and 4) control group. Following the familiarization sessions, a more consistent protocol with an intensity of 65% vo2 max was performed for 12 weeks. Afterward, half of the mice in each group received acute exercise training with an intensity of 70-75% of vo2 max at the age of 20 weeks, until reaching the disability level. Finally, the levels of inflammatory markers were measured using special kits. Our findings depicted that inflammatory factors such as CPR, TNF-α, IL-6, and IL-7 were not affected by endurance protocol (P > 0.05), whereas, they were significantly increased by acute exercise training (P > 0.05). Additionally, we found that RES supplements led to a decrease in CPR and IL-6 levels, while not affecting TNF-α and IL-17 levels. According to available evidence, RES appears to have anti-inflammatory and protective effects during exercise by reducing inflammatory factors. Further studies are required to clarify the role of trans-resveratrol supplementation after exercise training.","PeriodicalId":416671,"journal":{"name":"Iranian Journal of Pharmaceutical Research : IJPR","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122890569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Interaction of mTOR and iNOS pathways in protection against Ischemia/Reperfusion injury mTOR和iNOS通路对缺血/再灌注损伤的相互作用

Iranian Journal of Pharmaceutical Research : IJPR Pub Date : 2019-05-01 DOI: 10.22037/ijpr.2019.1100680

M. Arabian, N. Aboutaleb, M. Ajami, R. Habibey

{"title":"Interaction of mTOR and iNOS pathways in protection against Ischemia/Reperfusion injury","authors":"M. Arabian, N. Aboutaleb, M. Ajami, R. Habibey","doi":"10.22037/ijpr.2019.1100680","DOIUrl":"https://doi.org/10.22037/ijpr.2019.1100680","url":null,"abstract":"Chronic morphine (CM) treatment increases the phosphorylation of the mammalian target of rapamycin (mTOR), which confers neuroprotection against ischemia/reperfusion (I/R) injury. Besides its important regulatory role in the proliferation, metabolism, and survival of cells, the mTOR is critically involved in intracellular signaling events during I/R injury. In the present study, we investigated the interaction between the expressions of the mTOR and inducible nitric oxide synthase (iNOS) and their possible protective effects on hippocampal neurons against I/R injury in morphine-dependent mice. Additive doses of morphine were administered for 5 days to BALB/c mice so as to induce CM preconditioning before I/R injury. Global brain ischemia was induced via the occlusion of bilateral common carotid arteries for 30 min. CM attenuated iNOS expression, NO production, and malondialdehyde activity in the hippocampal tissue. Pretreatment with rapamycin, the inhibitor of mTOR, abolished all the above mentioned effects of CM. These findings suggested that CM acted through the mTOR signaling pathways to regulate iNOS expression and oxidative state in the hippocampal tissue after I/R injury.","PeriodicalId":416671,"journal":{"name":"Iranian Journal of Pharmaceutical Research : IJPR","volume":"71 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116515883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Characterization of Novel Fragment Antibodies Against TNF-alpha Isolated Using Phage Display Technique 利用噬菌体展示技术分离抗tnf - α的新型片段抗体的鉴定

Iranian Journal of Pharmaceutical Research : IJPR Pub Date : 2019-05-01 DOI: 10.22037/ijpr.2019.1100646

A. Alizadeha, Maryam Hamzeh-Mivehroud, E. Haddad, Nazanin Haddad, M. Sharifi, Samin Mohammadi, Samira Pourtaghi-anvarian, S. Dastmalchi

{"title":"Characterization of Novel Fragment Antibodies Against TNF-alpha Isolated Using Phage Display Technique","authors":"A. Alizadeha, Maryam Hamzeh-Mivehroud, E. Haddad, Nazanin Haddad, M. Sharifi, Samin Mohammadi, Samira Pourtaghi-anvarian, S. Dastmalchi","doi":"10.22037/ijpr.2019.1100646","DOIUrl":"https://doi.org/10.22037/ijpr.2019.1100646","url":null,"abstract":"Tumor necrosis factor alpha (TNF-α) is an inflammatory cytokine which plays crucial roles in pathogenesis of inflammatory diseases. The current study aimed to investigate the binding abilities of I44 and I49 domain antibodies to TNF-α. The dAbs were expressed in bacterial expression system and purified by affinity chromatography using Ni-sepharose column. The expression and purity of the proteins were evaluated using western blotting and SDS-PAGE techniques, respectively. ELISA experiment showed that I44 and I49 dAbs bind to TNF-α with the binding constants (Kd) of 5.18 ± 1.41 and 2.42 ± 0.55 µM, respectively. The inhibitory effect of dAbs on TNF-α biological effect was determined in MTT assay in which I44 and I49 prevented TNF-α cell cytotoxicity with IC50 values of 6.61 and 3.64 µM, respectively. The identified anti-TNF-α dAbs could bind to and inhibit TNF-α activity. The dAbs activities can be attributed to their ability to establish hydrogen bonds as well as hydrophobic contacts with TNF-α. The results of the current study can pave the way for further structural studies in order to introduce new more potent anti-TNF-α antibodies.","PeriodicalId":416671,"journal":{"name":"Iranian Journal of Pharmaceutical Research : IJPR","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132213603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Genetic Polymorphism of CYP2C19 in Pakistani Population 巴基斯坦人群CYP2C19基因多态性研究

Iranian Journal of Pharmaceutical Research : IJPR Pub Date : 2019-05-01 DOI: 10.22037/IJPR.2019.1100644

S. Riaz, Sadia Muhammad Din, Muhammad Usman Tareen, F. Tariq, Yusra Latif, S. Siddiqi, Aneesa Sultan, A. Mansoor

{"title":"Genetic Polymorphism of CYP2C19 in Pakistani Population","authors":"S. Riaz, Sadia Muhammad Din, Muhammad Usman Tareen, F. Tariq, Yusra Latif, S. Siddiqi, Aneesa Sultan, A. Mansoor","doi":"10.22037/IJPR.2019.1100644","DOIUrl":"https://doi.org/10.22037/IJPR.2019.1100644","url":null,"abstract":"CYP2C19 polymorphism is associated with pretreatment drug response prediction, metabolism, and disposition. Pakistan consists of a population comprising of various ethnic groups residing in different regions of the country each claiming diverse ethnic origins. The identification of CYP450 genotypic composition of these populations is therefore necessary to avoid adverse drug reactions in these individuals. The main objective of the study was to investigate the prevalence of CYP2C19*2 and CYP2C19*17 alleles in these ethnic groups. The study was conducted on one thousand and twenty-eight (n = 1028) healthy volunteers from nine ethnic groups of Pakistan namely Brusho (n = 28), Hazara (n = 102), Kalash (n = 64), Pathan (n = 170), Punjabi (n = 218), Saraiki (n = 59), Brahui (n = 118), Parsi (n = 90), and Sindhi (n = 179). DNA was extracted from leukocytes and analyzed by allele specific amplification polymerase chain reaction (ASA-PCR). Multi allelic polymorphism of CYP2C19 led to four distinct phenotypes identified as extensive metabolizer (EM), poor metabolizer (PM), intermediate metabolizer (IM), and ultra-rapid metabolizer (UM). Over all, the percentage of predicted poor metabolizer allele was 29.0% compared to UM allele (23.70%). Among the studied groups, Saraiki and Brahui showed highest percentage of PM allele (40%, 36%) whereas Parsi and Hazara had highest percentage of UM allele (37% and 30% respectively). In conclusion, the high allele frequency of PM (CYP2C19*2 and *17) in Pakistani population led to the recommendation of a pre-treatment test to monitor drug response and dosage (personalized medicine) to avoid post-treatment adverse drug reactions.","PeriodicalId":416671,"journal":{"name":"Iranian Journal of Pharmaceutical Research : IJPR","volume":"53 211 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125951127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

In-vitro Morphological Assessment of Apoptosis Induced by Nimbolide; A Limonoid from Azadirachta Indica (Neem Tree) Nimbolide诱导细胞凋亡的体外形态学研究印楝树中的一种柠檬素

Iranian Journal of Pharmaceutical Research : IJPR Pub Date : 2019-05-01 DOI: 10.22037/ijpr.2019.2391

M. Kashif, Yawon Hwang, Wun-Jae Kim, Gonhyung Kim

{"title":"In-vitro Morphological Assessment of Apoptosis Induced by Nimbolide; A Limonoid from Azadirachta Indica (Neem Tree)","authors":"M. Kashif, Yawon Hwang, Wun-Jae Kim, Gonhyung Kim","doi":"10.22037/ijpr.2019.2391","DOIUrl":"https://doi.org/10.22037/ijpr.2019.2391","url":null,"abstract":"The present study was designed to investigate the in-vitro morphological assessment of apoptotic effect caused by nimbolide on the selected cancer cell lines (DU-145, PC-3, A-549) and normal cell lines (NIH3T3, CCD-18Co). The cells were grown in 6 well tissue culture plates after treatment with different concentrations of nimbolide and untreated control cells. Apoptotic and necrotic cells were measured using Hoechst 33342 and propidium iodide dual staining through a fluorescent microscope and also by staining with annexin V and propidium iodide through flow cytometric analysis. The activity of caspase 3, 8, and 9 was measured by caspases colorimetric assay kits. The number of apoptotic and necrotic cells were significantly higher in all selected cancer cell lines treated with nimbolide as compared with untreated control cells, whereas in normal cell lines no significant difference was observed between nimbolide treated and untreated cells. The activity of caspase 3, 8, and 9 was also significantly higher in all cancer cell lines treated with nimbolide as compared with untreated control cells while it did not change significantly in normal cell lines as compared with untreated control. The results of the present study suggested that nimbolide induced apoptosis only in cancer cells without affecting the normal cells and one of the apoptosis inducing mechanism is through the activation of caspases signaling pathways. Therefore, nimbolide may be a novel promising candidate as an anticancer drug in future.","PeriodicalId":416671,"journal":{"name":"Iranian Journal of Pharmaceutical Research : IJPR","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122625022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

A Dispersive Liquid–Liquid Micro–Extraction Technique for the Pre–concentration and Quantification of Vitamin D3 in Milk and Yogurt Samples Using a Non-Aqueous HPLC Method 非水相高效液相色谱法测定牛奶和酸奶样品中维生素D3含量的分散液液微萃取技术

Iranian Journal of Pharmaceutical Research : IJPR Pub Date : 2019-05-01 DOI: 10.22037/ijpr.2019.1100634

M. Ghalebi, E. Tamizi, Shirin Ahmadi, Ahad Sheikhloo, M. Nemati

{"title":"A Dispersive Liquid–Liquid Micro–Extraction Technique for the Pre–concentration and Quantification of Vitamin D3 in Milk and Yogurt Samples Using a Non-Aqueous HPLC Method","authors":"M. Ghalebi, E. Tamizi, Shirin Ahmadi, Ahad Sheikhloo, M. Nemati","doi":"10.22037/ijpr.2019.1100634","DOIUrl":"https://doi.org/10.22037/ijpr.2019.1100634","url":null,"abstract":"In present study, a DLLME-HPLC-UV method was developed and validated for the extraction, pre–concentration, and subsequently quantification of vitamin D3 (Vit D3) in milk and yogurt samples. In order to be able to extract Vit D3 from studied samples efficiently, the DLLME procedure was optimized with respect to the parameters affecting the extraction efficacy, where acetonitrile (2 mL as disperser solvent) resulting from the protein precipitation procedure was mixed with 80 µL carbon tetrachloride (as an extraction solvent) respectively. The extracted samples were quantitatively analyzed with a HPLC technique using a C8 column (250 mm × 4.6 mm, 5 μm) at room temperature (25 °C), mobile phase of acetonitrile/methanol (90:10% v/v) in isocratic elution mode at a flow rate of 1.2 mL/min and UV detection at 265 nm. The method validation results revealed that the method was linear in the concentration range of 2 to 60 ng/mL (r = 0.9997) with a LOD of 0.9 ng/mL and LLOQ of 2 ng/mL; the method was accurate (-2.1% ≤ RE% ≤ +0.6%) and precise (1.2% ≤RSD% ≤ 11.3%) and its recovery was in the range of 86.6 to 113.3%. The obtained results indicated that the method could be utilized as an easy to use technique for the monitoring Vit D3 in dairy products, especially milk and yogurt samples.","PeriodicalId":416671,"journal":{"name":"Iranian Journal of Pharmaceutical Research : IJPR","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122242414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Chemical Composition, Antioxidant, Anticholinesterase and Anti-Tyrosinase Activities of Essential Oils of Two Sideritis Species from Turkey 土耳其两种黄芪精油的化学成分、抗氧化活性、抗胆碱酯酶和抗酪氨酸酶活性

Iranian Journal of Pharmaceutical Research : IJPR Pub Date : 2019-05-01 DOI: 10.22037/ijpr.2019.1100657

Ebru Deveci, Gülsen Tel‐Çayan, Özlem Usluer, M. Emin Duru

{"title":"Chemical Composition, Antioxidant, Anticholinesterase and Anti-Tyrosinase Activities of Essential Oils of Two Sideritis Species from Turkey","authors":"Ebru Deveci, Gülsen Tel‐Çayan, Özlem Usluer, M. Emin Duru","doi":"10.22037/ijpr.2019.1100657","DOIUrl":"https://doi.org/10.22037/ijpr.2019.1100657","url":null,"abstract":"Sideritis species have been known as medicinal plants since ancient times, and used as tea in Mediterranean countries such as Turkey, Greece, and Spain. They are also used for the treatment of several ailments such as a cough, common cold, and gastrointestinal disorders. The aim of the present study was to perform the chemical composition, antioxidant, anticholinesterase, and anti-tyrosinase activities of the essential oils of Sideritis albiflora and S. leptoclada. β-caryophyllene (21.2%) and Germacrene D (17.9%) were identified as the major compounds in S. albiflora and S. leptoclada essential oils, respectively. The essential oil of S. albiflora showed the highest lipid peroxidation inhibitory (IC50: 73.8 ± 0.8 µg/mL), DPPH free radical scavenging (28.3±0.1%), ABTS cation radical scavenging (IC50: 50.6 ± 1.0 µg/mL), reducing power (A0.05: 181.7 ± 0.6 µg/mL), acetylcholinesterase (22.1 ± 0.4%), butyrylcholinesterase (IC50: 157.2 ± 0.9 µg/mL) and tyrosinase (15.2 ± 0.4%) inhibitory activities. Moreover, S. albiflora essential oil had rich total phenolic and flavonoid contents indicating 41.5 ± 0.8 µg PEs/mg and 21.4 ± 1.0 µg QEs/mg respectively. This study suggests that consumption of Sideritis species as tea may protect one against melanogenesis, amnesia, and oxidative stress without any observable side effect.","PeriodicalId":416671,"journal":{"name":"Iranian Journal of Pharmaceutical Research : IJPR","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128820034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Fingerprint Analysis and Quantitative Determination of Fourteen Active Components in the Traditional Chinese Medicinal Preparation Feiyangchangweiyan Capsule by HPLC-DAD-ESI-MS/MS HPLC-DAD-ESI-MS/MS法对中药制剂飞阳长胃炎胶囊中14种有效成分的指纹图谱分析及定量测定

Iranian Journal of Pharmaceutical Research : IJPR Pub Date : 2019-05-01 DOI: 10.22037/ijpr.2019.1100649

Dan Zhou, Qian Yang, Zhe Yu, Ying‐Ying Chang, T. Tian, Zhi-hui Shi, H. Dong, Hua Li, Jun Guo, Siwang Wang

引用次数: 6

Effect of Purgative Manna on Neonatal Hyperbilirubinemia: A Systematic Review and Meta-analysis 泻性甘露对新生儿高胆红素血症的影响:系统回顾和荟萃分析

Iranian Journal of Pharmaceutical Research : IJPR Pub Date : 2019-02-05 DOI: 10.22037/ijpr.2019.2388

F. Sajedi, Shiva Fatollahierad

{"title":"Effect of Purgative Manna on Neonatal Hyperbilirubinemia: A Systematic Review and Meta-analysis","authors":"F. Sajedi, Shiva Fatollahierad","doi":"10.22037/ijpr.2019.2388","DOIUrl":"https://doi.org/10.22037/ijpr.2019.2388","url":null,"abstract":"The aim of this systematic review was to evaluate the effect of purgative manna on the unconjugated hyperbilirubinemia in neonates. Pubmed, Scopus, Chochrane library, Iranmedex and Google scholar were last searched in February 2017. Randomized controlled trials that evaluated the effect of purgative manna on the treatment of neonatal hyperbilirubinemia were included in the review. For meta-analysis, weighted mean difference (WMD) with 95% confidence interval (CI) was used. The outcomes of interests were serum bilirubin levels and length of hospital stay in neonates with jaundice. Seven randomized controlled trials with 812 neonates were eligible to be included in this systematic review. The meta-analysis included six of seven controlled trials. Bilirubin levels were significantly lower at 12 h (WMD: -1.48, 95% CI: -2.31 to -0.65), 24 h (WMD: -2.47, 95% CI: -3.22 to -1.71), 36 h (WMD: -2.83, 95% CI: -4.87 to -0.80), 48 h (WMD: -1.49, 95% CI: -2.36 to -0.63) and 72 h (WMD: -0.68, 95% CI: -1.28 to -0.08) following intervention in purgative manna group. Length of hospital stay was also decreased in purgative manna group (WMD: -0.93, 95% CI: -1.35 to -0.50). Finally, purgative manna administration decreased serum bilirubin level and length of hospital stay in neonates with unconjugated hyperbilirubinemia. More studies are needed to evaluate the efficacy, dosage, and side effects of purgative manna.","PeriodicalId":416671,"journal":{"name":"Iranian Journal of Pharmaceutical Research : IJPR","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115397773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Preparation, In-Vitro Characterization and Pharmacokinetic Evaluation of Brij Decorated Doxorubicin Liposomes as a Potential Nanocarrier for Cancer Therapy 作为肿瘤治疗潜在纳米载体的白蜡修饰阿霉素脂质体的制备、体外表征及药动学评价

Iranian Journal of Pharmaceutical Research : IJPR Pub Date : 2018-12-01 DOI: 10.22037/IJPR.2018.2373

M. Fazel, Marjan Daeihamed, M. Osouli, Ameneh Almasi, A. Haeri, S. Dadashzadeh

{"title":"Preparation, In-Vitro Characterization and Pharmacokinetic Evaluation of Brij Decorated Doxorubicin Liposomes as a Potential Nanocarrier for Cancer Therapy","authors":"M. Fazel, Marjan Daeihamed, M. Osouli, Ameneh Almasi, A. Haeri, S. Dadashzadeh","doi":"10.22037/IJPR.2018.2373","DOIUrl":"https://doi.org/10.22037/IJPR.2018.2373","url":null,"abstract":"The aim of current study was to investigate the effect of Brij decoration of liposomes on in-vitro and in-vivo characteristics of the nanocarriers. Two hydrophilic Brij surfactants (Brij 35 and Brij 78) with almost similar molecular weight but differing in acyl chain were incorporated into liposomal bilayers at two percentages (5% and 10%). Conventional liposomes (CL) containing egg phosphatidylcholine and cholesterol as well as Brij-enriched liposomal dispersions were prepared and characterized. In-vivo pharmacokinetics of various liposomal formulations and drug solution (six groups) was studied after intravenous administration to rats. Conventional and Brij enriched doxorubicin (DOX) liposomes had small size within 82-97 nm and showed homogenous distribution (PDI < 0.1). Drug encapsulation was higher than 97% in all liposomes. The drug release profiles proved sustained DOX release from various formulations. Based on the results of in-vivo studies, all five liposomes increased drug exposure and plasma concentration in comparison to free drug. However, DOX liposomes enriched with 5% of either Brij 35 or Brij 78 showed higher AUC values and lower clearance. Overall, Brij surfactants (5% of bilayer lipids) could be potentially used to improve liposomal pharmacokinetic parameters.","PeriodicalId":416671,"journal":{"name":"Iranian Journal of Pharmaceutical Research : IJPR","volume":"89 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128722170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7