Bifidobacteria and Microflora最新文献

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Isolation and Identification of Bifidobacterium spp.in Commercial Yogurts Sold in Europe 在欧洲销售的商品酸奶中双歧杆菌的分离和鉴定
Bifidobacteria and Microflora Pub Date : 1900-01-01 DOI: 10.12938/BIFIDUS1982.12.1_39
H. Iwana, H. Masuda, T. Fujisawa, Hideki Suzuki, T. Mitsuoka
{"title":"Isolation and Identification of Bifidobacterium spp.in Commercial Yogurts Sold in Europe","authors":"H. Iwana, H. Masuda, T. Fujisawa, Hideki Suzuki, T. Mitsuoka","doi":"10.12938/BIFIDUS1982.12.1_39","DOIUrl":"https://doi.org/10.12938/BIFIDUS1982.12.1_39","url":null,"abstract":"A new selective medium, galactose (GL) agar, was developed for the enumeration of Bifidobacterium spp. in yogurts . This medium contains lithium chloride (0.4 g/1) and galactose (10 g/l) as selective inhibitory agents. The viable counts of bifidobacteria in the commercial yogurts sold in Europe were determined by using GL agar. From five of eight commercial yogurts tested , bifidobacteria were detected in 104-107 per ml, while from the remaining three they were not detected. Five Bifidobacterium strains isolated were identified on carbohydrate fermentation patterns and DNA-DNA homology. Only one strain was classified B. longum and the remaining four strains as B. animalis.","PeriodicalId":414713,"journal":{"name":"Bifidobacteria and Microflora","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132459946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 72
Über den Bifidusfaktor Lactulose 关于乳糖菌的双纤维性因素
Bifidobacteria and Microflora Pub Date : 1900-01-01 DOI: 10.12938/BIFIDUS1982.5.1_3
F. Petuely
{"title":"Über den Bifidusfaktor Lactulose","authors":"F. Petuely","doi":"10.12938/BIFIDUS1982.5.1_3","DOIUrl":"https://doi.org/10.12938/BIFIDUS1982.5.1_3","url":null,"abstract":"","PeriodicalId":414713,"journal":{"name":"Bifidobacteria and Microflora","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132541811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Protective Effect of Bifidobacterium-Monoassociation against Lethal Activity of Escherichia coli 双歧杆菌对大肠杆菌致死活性的保护作用
Bifidobacteria and Microflora Pub Date : 1900-01-01 DOI: 10.12938/BIFIDUS1982.1.1_55
S. Yamazaki, H. Kamimura, H. Momose, T. Kawashima, K. Ueda
{"title":"Protective Effect of Bifidobacterium-Monoassociation against Lethal Activity of Escherichia coli","authors":"S. Yamazaki, H. Kamimura, H. Momose, T. Kawashima, K. Ueda","doi":"10.12938/BIFIDUS1982.1.1_55","DOIUrl":"https://doi.org/10.12938/BIFIDUS1982.1.1_55","url":null,"abstract":"Germ-free ICR mice monoassociated with Bifidobacterium longum (GB mice) lived longer than non-treated germ-free (GF) mice after intragastric or intravenous (i.v.) administration of a high dose of viable E. coli, or i.v. injection of endotoxin. The number of viable E. coli and the endotoxin level in various organs at 24 hr post injection were significantly lower in GB than GF mice . Antilethal activity was not elicited by the feeding of heat-killed B. longum. The antilethal effect was observed 3, but not less than 2, weeks after the association of B. longum. Furthermore, the effect was not observed when GF-athymic nude mice (BALB/c background) were used. After intragastric administration of a sublethal dose, the number of viable E. coli in organs of GB mice decreased to an undetectable level within 7 days, while the organisms were persistently isolated in GF mice. In both groups, many (109 v.c./g) E. coli persisted in the intestine. These results suggest that B . longum organisms monoassociated with GF mice generate systemic resistance to the invading E. coli, and increase the resistance to the lethal activity of endotoxin .","PeriodicalId":414713,"journal":{"name":"Bifidobacteria and Microflora","volume":"5 3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116786041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 52
Characterization of Growth and Inulinase Production by Bifidobacterium spp. on Fructooligosaccharides 双歧杆菌对低聚果糖生长和产菊粉酶的研究
Bifidobacteria and Microflora Pub Date : 1900-01-01 DOI: 10.12938/BIFIDUS1982.12.2_75
R. Mckellar, H. Modler, J. Mullin
{"title":"Characterization of Growth and Inulinase Production by Bifidobacterium spp. on Fructooligosaccharides","authors":"R. Mckellar, H. Modler, J. Mullin","doi":"10.12938/BIFIDUS1982.12.2_75","DOIUrl":"https://doi.org/10.12938/BIFIDUS1982.12.2_75","url":null,"abstract":"A total of 19 species of Bifidobacterium were screened for the ability to grow with fructooligosaccharides (FOS) as the carbohydrate source. Several strains of animal origin (B. thermophilum, B. minimum, and B. cuniculi) grew significantly better than strains from human source on inulin (degree of polymerization (DP) •† 15). These strains grew equally well on inulin from several sources, but were unable to metabolize levan, a polymer of ƒÀ(2-6)-D-fructose. Best growth was obtained on FOS preparations containing a significant proportion of low-molecular-weight (DP<5) carbohydrates. Strains which grew best on inulin required the presence of this FOS for maximal induction of the exo-inulinase. These results suggest that animal strains of bifidobacteria may be used in conjunction with short-chain FOS as a supplement to animal feed.","PeriodicalId":414713,"journal":{"name":"Bifidobacteria and Microflora","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123941415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 25
Antitumor Immunity of Gut and the Role of Oral Immunotherapy in the Multidisciplinary Treatment of Digestive Organ Cancer 肠道抗肿瘤免疫及口服免疫治疗在消化器官肿瘤多学科治疗中的作用
Bifidobacteria and Microflora Pub Date : 1900-01-01 DOI: 10.12938/BIFIDUS1982.8.2_59
Y. Nio, T. Tobe
{"title":"Antitumor Immunity of Gut and the Role of Oral Immunotherapy in the Multidisciplinary Treatment of Digestive Organ Cancer","authors":"Y. Nio, T. Tobe","doi":"10.12938/BIFIDUS1982.8.2_59","DOIUrl":"https://doi.org/10.12938/BIFIDUS1982.8.2_59","url":null,"abstract":"Associated with the gut is a broad immune system, and the various tissues which make up this system are termed gut-associated lymphoid tissue (GALT) which includes lymph nodes, the appendix, lymphoid nodules , Peyer's patches, intraepithelial lymphocytes, and lamina propria lymphocytes (16) . GALT is thought to play an important role in the immunological surveillance system against micoorganisms or dietary antigens entering the gut . GALT also appears to play an important role in the antitumor immunity of the gut , because natural killer (NK) cells and T cells are widely distributed in the epithelium and lamina propria of the gut. However, the role of GALT against digestive organ cancer is still unclear (1, 6). GALT works independently of systemic lymphoid tissue and has some important characteristics, such as a secretory immunoglobulin system, a homing phenomenon of T and B blasts, and induction of a tolerance to orally administered antigens. The most important phenomenon is that the local immunity of the gut is established by oral immunization, but not by systemic immunization (4, 15) .","PeriodicalId":414713,"journal":{"name":"Bifidobacteria and Microflora","volume":"104 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124042979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Effect of Menstrual Cycle and Different Age on Vaginal Microflora of Healthy Women 月经周期及不同年龄对健康女性阴道菌群的影响
Bifidobacteria and Microflora Pub Date : 1900-01-01 DOI: 10.12938/BIFIDUS1982.11.1_33
T. Fujisawa, Y. Benno, T. Mitsuoka
{"title":"Effect of Menstrual Cycle and Different Age on Vaginal Microflora of Healthy Women","authors":"T. Fujisawa, Y. Benno, T. Mitsuoka","doi":"10.12938/BIFIDUS1982.11.1_33","DOIUrl":"https://doi.org/10.12938/BIFIDUS1982.11.1_33","url":null,"abstract":"The composition of vaginal microflora of forty healthy women whose ages ranged from 20 to 50 years (menstrual cycle: estrogenic phase, 15 persons; luteal phase, 25 persons) was anaerobically and aerobically determined . The total numbers in the vaginal specimens obtained from all the subjects were approximately 109 CFU/g of vaginal secretions. Numbers of anaerobic bacteria in the vaginal specimens were higher than those of aerobic bacteria . Lactobacilli were the predominant microorganisms in the vaginal specimens . The numbers of bacteroides , streptococci, and Gardnerella vaginalis, and incidences of staphylococci and peptostreptococci were increased with aging whereas the numbers of lactobacilli decreased with aging .","PeriodicalId":414713,"journal":{"name":"Bifidobacteria and Microflora","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129581379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Virulence Factors of Clostridium difficile and Its Pathogenesis in Intestinal Infection in Man 艰难梭菌的毒力因子及其在人肠道感染中的致病机制
Bifidobacteria and Microflora Pub Date : 1900-01-01 DOI: 10.12938/BIFIDUS1982.12.1_1
S. Kamiya, Shinichi Nakamura
{"title":"Virulence Factors of Clostridium difficile and Its Pathogenesis in Intestinal Infection in Man","authors":"S. Kamiya, Shinichi Nakamura","doi":"10.12938/BIFIDUS1982.12.1_1","DOIUrl":"https://doi.org/10.12938/BIFIDUS1982.12.1_1","url":null,"abstract":"Clostridium difficile was isolated in 1935 by Hall and O'Toole (29) from the feces of healthy neonate, and the microorganism was first designated Bacillus difficilis. The 'difficilis' was originated from the difficulty in cultivation of the microorganism. They showed that the microorganism had lethality to rabbit or guinea pig and edema-inducing activity. Later the microorganism was classified as Clostridium difficile in 1938 by Prevot (71) . The pathogenicity of C. difficile to human had not been reported until when association of C. difficile with human pseudomembranous colitis (PMC) was demonstrated in 1978. In 1893, Finney (19) first reported an occurrence of PMC in the patient with peptic ulcer after gastroenterostomy. The number of cases with PMC increased in parallel to an increase of use of antibiotics in the 1970s. Although Staphylococcus aureus was speculated to be a causative agent for PMC, there was no determinative evidence for the hypothesis. In 1977, Larson et al (48) reported that clostridial toxin was causative agent for PMC, and in 1978 toxigenic C. difficile was identified as a cause of PMC by Bartlett et al (3), George et al (23) and Larson et al (49) . The isolation rate of C. difficile from feces of healthy adults was 0-12.8%, and that of the patients with PMC was 80-100% (59) . It is considered that change of intestinal flora by administration of antimicrobial agent and following selection of toxin-producing C. difficile are background for the occurrence of PMC. Virulence factors of C. difficile and its pathogenesis in intestinal infection in man are discussed in this review by indicating not only previous reports in the literature but also our own experimental data.","PeriodicalId":414713,"journal":{"name":"Bifidobacteria and Microflora","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129944611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
An Automatic System for Enumeration of Colony-Forming Units by the Surface Plate Method 平板法菌落形成单位自动计数系统
Bifidobacteria and Microflora Pub Date : 1900-01-01 DOI: 10.12938/BIFIDUS1982.8.2_87
Y. Benno, Shigenari Kukisaki, Tomotari Mitsuok
{"title":"An Automatic System for Enumeration of Colony-Forming Units by the Surface Plate Method","authors":"Y. Benno, Shigenari Kukisaki, Tomotari Mitsuok","doi":"10.12938/BIFIDUS1982.8.2_87","DOIUrl":"https://doi.org/10.12938/BIFIDUS1982.8.2_87","url":null,"abstract":"A device for mechanical dilution and application of specimens and counting microbial colonies was developed. The device consists of a transport apparatus which transports petri dishes from one side of the apparatus to the other in such a way that the plate and lid of petri dishes are entirely separated at a particular stage. A diluting and inoculating system performs 10-fold dilutions of specimens from 10-1 to 10-8 dilutions and inoculation of the diluents. The inoculum is applied to the agar surface of preselected petri plates by an application system. Both a diluting and inoculating system and an application system can be connected to the apparatus. In this way a maximum of 25 agar plates can be handled. This new automatic system for enumeration of colony-forming units by the surface plate method is more accurate and reliable than the customary conventional methods.","PeriodicalId":414713,"journal":{"name":"Bifidobacteria and Microflora","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130684896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Deoxyribonucleic Acid Homology Relationships between the Antibiotic-resistant Bifidobacteria and Each Original Strain 耐药双歧杆菌与各原菌株脱氧核糖核酸同源性研究
Bifidobacteria and Microflora Pub Date : 1900-01-01 DOI: 10.12938/BIFIDUS1982.9.2_131
Mikiro Imamvra, T. Fujisawa, T. Mitsuoka
{"title":"The Deoxyribonucleic Acid Homology Relationships between the Antibiotic-resistant Bifidobacteria and Each Original Strain","authors":"Mikiro Imamvra, T. Fujisawa, T. Mitsuoka","doi":"10.12938/BIFIDUS1982.9.2_131","DOIUrl":"https://doi.org/10.12938/BIFIDUS1982.9.2_131","url":null,"abstract":"Six antibiotic-resistant bifidobacteria (bif-R) strains were compared with each original strain by deoxyribonucleic acid (DNA)/ DNA homology. The bif-R strains had extremely high levels of over 93% homology to each 3H-labeled original strain.","PeriodicalId":414713,"journal":{"name":"Bifidobacteria and Microflora","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128771920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Immunopotentiation of the Small Intestine of Weaning Piglets by Peptidoglycan Derived from Bifidobacterium thermophilum 嗜热双歧杆菌肽聚糖对断奶仔猪小肠免疫增强作用的研究
Bifidobacteria and Microflora Pub Date : 1900-01-01 DOI: 10.12938/BIFIDUS1982.10.1_1
S. Namioka, Takashi Sasaki, Y. Maede
{"title":"Immunopotentiation of the Small Intestine of Weaning Piglets by Peptidoglycan Derived from Bifidobacterium thermophilum","authors":"S. Namioka, Takashi Sasaki, Y. Maede","doi":"10.12938/BIFIDUS1982.10.1_1","DOIUrl":"https://doi.org/10.12938/BIFIDUS1982.10.1_1","url":null,"abstract":"One of the most serious problems in large-scale commercial pig farms is the post weaning diarrhea syndrome in piglets aged 4-6 weeks. It is clear that the major causative agent of the syndrome is an enteropathogenic Escherichia coli (5, 7, 13, 15, 16). M oreover, it is well known that peptidoglycan , which is derived from the cell wall of gram-positive organisms, is an immunopotentiator; peptidoglycan has been used to treat various kinds of immunodeficiencies , especially in humans (2, 3, 10, 11, 18). The purpose of the present study was to investigate the immunological effect of peptidoglycan from Bifidobacterium thermophilum in piglets and see whether an enhancement of the local immune response is obtained or not in weaning piglets aged 5-6 weeks, as well as to investigate changes in the size of the E . coli population in various parts of the small intestine.","PeriodicalId":414713,"journal":{"name":"Bifidobacteria and Microflora","volume":"214 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121450861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 14
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