Journal of Plant Studies最新文献

{"title":"In vitro and in vivo Assay for Assessment of the Biological Control Potential of Streptomyces sp. KT","authors":"Tatsuya Ohike, Minori Maeda, T. Matsukawa, M. Okanami, S. Kajiyama, T. Ano","doi":"10.5539/JPS.V7N1P10","DOIUrl":"https://doi.org/10.5539/JPS.V7N1P10","url":null,"abstract":"Rhizoctonia solani is fungal plant pathogen that infects many different host plants. Recently, biological control agents that are friendly to the environment and ecosystems have attracted much attention as an alternative to the use of chemical fungicide which have been used worldwide to control soil borne pathogens including R. solani. In this study, 53 strains of actinomycetes isolated from environmental soils, and antifungal activities of them were assessed by the dual culture assay. Strain KT showed strong inhibitory activities against 8 phytopathogenic fungi. A great suppressive effect on R. solani growth was observed in the inoculation test of plants using cucumber and chin-geng-sai. In addition, infection of Bipolaris oryzae also could be suppressed in the detached leaf assay using oats. As a result of genetic analysis, it was shown that KT was a species closely related to Streptomyces lavenduligriseus NRRL B-3173T. However, as far as we know, there is no report for biological control agents using S. lavenduligriseus. This study suggests that the strain KT may useful as biological control agents to suppress various crop diseases.","PeriodicalId":410634,"journal":{"name":"Journal of Plant Studies","volume":"156 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115002029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic Fingerprinting of Citrus Cultivars and Related Genera Using HPLC and Multivariate Analysis","authors":"T. Matsukawa, N. Nito","doi":"10.5539/JPS.V7N1P1","DOIUrl":"https://doi.org/10.5539/JPS.V7N1P1","url":null,"abstract":"Citrus taxonomy is very complex and confusing, because of asexual reproduction and sexual compatibility between Citrus and related genera. Metabolic diversity was studied in Citrus, Poncirus and Fortunella cultivars by the high performance liquid chromatography technique combined with multivariate statistical analysis. Chromatograms obtained from cultivars of the same species showed similar elution profiles. These results suggested that metabolic profiles carry characteristics of hybrid origin. To confirm the similarities among the Citrus species and their cultivars, multivariate statistical analysis was applied to the chromatograms. According to hierarchical cluster analysis, all cultivars used in this study were divided into three major groups, which largely correspond to pummelo, mandarin and lemon. Hybrids were clustered together with their hybrid origin or their related cultivars. Our results indicated that the metabolic fingerprinting method provides an insight into the phylogenic relationships among Citrus species and cultivars.","PeriodicalId":410634,"journal":{"name":"Journal of Plant Studies","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123731369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of Advanced Glycation End Products Formation by Mangifera indica Leaf Extract","authors":"Kimihisa Itoh, K. Murata, N. Sakaguchi, Kohei Akai, Tomoka Yamaji, Kohsuke Shimizu, K. Isaki, T. Matsukawa, S. Kajiyama, M. Fumuro, M. Iijima, H. Matsuda","doi":"10.5539/JPS.V6N2P102","DOIUrl":"https://doi.org/10.5539/JPS.V6N2P102","url":null,"abstract":"The purpose of this study was to examine an inhibitory effect of mango lea f extracts on advanced glycation end products (AGEs) formation and to identify these active ingredients, and also to investigate a relationship between leaves maturation and the inhibitory activity. A methanolic extract of old dark green mango leaf extract (OML-ext) exhibited an inhibitory activity of AGEs formation in nonenzymatic glycation of albumin. The inhibitory activity of OML-ext was attributable to 3-C- β - D -glucosyl-2,4,4’,6-tetrahydroxybenzophenone ( 1 ), mangiferin ( 2 ) and chlorophyll. Inhibitory effect of young dark reddish brown mango leaf extract (YDL-ext) on AGEs formation was similar to that of OML-ext. The inhibitory activity of YDL-ext was attributable to 1 and 2 , in addition, a part of the the activity of YDL-ext due to anthocyanins whose content is highest in young dark reddish brown mango leaves. Considering the amounts of leaves obtained from pruning, old dark green leaves may be a reasonable natural resource for the preparation of ingredients with inhibitory activity of AGEs formation.","PeriodicalId":410634,"journal":{"name":"Journal of Plant Studies","volume":"49 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129242387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Height-diameter relationships in longleaf pine and four swamp tree species","authors":"Xiongwen Chen, D. Brockway","doi":"10.5539/JPS.V6N2P94","DOIUrl":"https://doi.org/10.5539/JPS.V6N2P94","url":null,"abstract":"The scaling relationship between height and diameter is important for understanding the dynamic patterns of tree growth and estimating the accrual of tree biomass. Metabolic ecology predicts that tree growth follows a universal scaling invariant relative to the height-diameter relationship (i.e., no variation based on taxonomy or resource availability). Comparing field data for different tree species across a range of site conditions should be an informative test of that prediction. Our results indicate that the scaling exponents of height and diameter for longleaf pine (Pinus palustris Mill.) vary at the four locations across its natural range. As for swamp trees, the scaling exponents for red maple (Acer rubrum L.) and river birch (Betula nigra L.) were consistent with that predicted by metabolic ecology; however, those for water tupelo (Nyssa aquatica L.) and bald cypress (Taxodium distichum (L.) Rich) were not. Our study confirms that high plasticity and variation in allometric scaling of the tree height and diameter relationship may very well be the rule, rather than the exception.","PeriodicalId":410634,"journal":{"name":"Journal of Plant Studies","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133314290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Piliostigma reticulatum on the Vegetation Dynamic in Sudanian Zone of Burkina Faso","authors":"Y. Barthelemy, Abdoulaye Tyano, B. Bationo, B. Ouattara, J. Koala, J. M. Rasolodimby","doi":"10.5539/JPS.V6N2P77","DOIUrl":"https://doi.org/10.5539/JPS.V6N2P77","url":null,"abstract":"This study aims at identifying the effect of P. reticulatum on the vegetation regeneration dynamic in Sudanian fallows of Burkina Faso. Two fallows of about twenty years old have been considered. The point quadrat method was used for herbaceous inventory. Aboveground biomass has been estimated. Moreover, effect of P. reticulatum on the regeneration of others woody species has been studied under, at the limit and out of the shrub canopy. In north Sudanian zone, inventory recorded 39 herbaceous species of which 23 species under the canopy and 16 in open grassland, distributed in 12 families and 22 genera. In south Sudanian zone, species diversity is 41 species from which 23 under the canopy and 18 out of, spread in 16 families and 26 genera. Species higher than 80 cm have been observed only under P. reticulatum canopy in south and north Sudanian zone. In addition, no species of striga met in open sunlight has been observed under the shrub canopy. East and West directions recorded the important proportions of species with individuals belonging to the stratum superior to 100 cm (3.13 and 4.81 % in north direction, 1.47 and 1.73% in south directions).","PeriodicalId":410634,"journal":{"name":"Journal of Plant Studies","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116055222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response of Assorted Maize Germplasm to the Maize Lethal Necrosis Disease in Kenya.","authors":"J. Sitta, F. Nzuve, F. Olubayo, C. Mutinda, W. Muiru, D. Miano, J. Muthomi, P. Leley","doi":"10.5539/JPS.V6N2P65","DOIUrl":"https://doi.org/10.5539/JPS.V6N2P65","url":null,"abstract":"Maize (Zea mays L.) is the most widely grown staple food crop in Sub Saharan Africa (SSA) and occupies more than 33 million hectares each year. The recent outbreak and rapid spread of the Maize Lethal Necrosis (MLN) disease has emerged as a great challenge to maize production, threatening food security for the majority of households in the Eastern Africa region with yield loss estimated to be 50-90%. The disease is a result of synergistic interaction between two viruses, Sugarcane mosaic virus (SCMV) and Maize chlorotic mottle virus (MCMV). The objective of this study was to identify maize genotypes with resistance to MLN. In season one, 73 maize genotypes comprising 25 inbred lines from research institutes, 30 lines from the International Maize and Wheat Improvement Centre (CIMMYT) and 18 farmer varieties were screened for resistance to MLN. In season 2, only 48 genotypes were screened after some of the inbred lines showed complete susceptibility to MLN. These genotypes were grown in three replications in a completely randomized design in polythene bags in the greenhouse at the University of Nairobi. The plants were artificially inoculated using a mixture of SCMV and MCMV. .Weekly MLN disease severity scores using a scale of 1 to 5 (1 = highly resistant and 5 = highly susceptible) and % MLN incidence were recorded and eventually converted into Area under Disease Progress Curve (AUDPC) to give an indication of the disease intensity over time. The plants were allowed to grow to flowering stage to observe the effect of the MLN on the maize productivity. Analysis of Variance revealed wide genetic variation among the genotypes ranging from resistant to highly susceptible. In season 1, three farmer varieties namely MLR2, MLR11 and MLR13 showed resistance to MLN with a mean severity score of 2. In season 2, MLN12, MLN17, MLN18, MLN19, and MLR4 showed low MLN severity ranging from 2-3. The genotypes MLR6, MLR9, MLR16 and MLR18 showed MLN severity of 3 and early maturity traits. This study also validated the presence of MLN resistance among some CIMMYT lines depicted to show resistance in previous studies. These resistant genotypes could serve as donors in the introgression of the resistance into the adapted Kenyan maize backgrounds. This will go a long way in ensuring sustainable maize productivity while improving the livelihoods of the small-scale farmers who form the bulk of the major maize producers in Kenya.","PeriodicalId":410634,"journal":{"name":"Journal of Plant Studies","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125086821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stress, Strain-Rate Analysis of Sub-Surface Driveway Plants","authors":"P. Greene, Virginia A. Greene","doi":"10.5539/JPS.V6N2P55","DOIUrl":"https://doi.org/10.5539/JPS.V6N2P55","url":null,"abstract":"Sub-surface driveway plants are strong enough to penetrate a macadam surface of thickness 7 – 9 cm. The mechanics of how the Taraxacum officinale accomplishes this feat remain a mystery. Using the Maxwell model for pavement yielding over time, data are presented which may shed some light on this phenomenon. The post-buckling behavior of the plant stalk is quantified. Euler bending and buckling theory enables calculation of the cellular stress field, compared to turgor pressure, indicating impending cell buckling. Post-buckling plastic strain of the plant stem is 19%. At the cell wall, the stress concentration factor is 3-times greater than the applied external field, so the cell’s internal turgor pressure is overwhelmed by imposed external stress. An Impulse Integral is developed for the surface whereby the product of applied FORCE times TIME is CONSTANT, in order to produce a given amount of surface deflection. Taraxacum officinale stems and leaf stalks are strong enough, in buckling mode, to lift and push apart the fractured macadam crater through which they erupt, but not strong enough to initially crack the surface. The purpose of this work is to determine the mechanisms underlying this unusual plant survival phenomenon, backed by quantified data.","PeriodicalId":410634,"journal":{"name":"Journal of Plant Studies","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114633950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Stability and Disease Resistance Analysis of Hrpzpsta Gene in Transgenic Soybean Lines","authors":"Miao Yu, Peiwu Wang, Yang Song, Yong-Qi Feng, J. Qu, Jie Rong, Mo Zhang, Zhuo Zhang","doi":"10.5539/JPS.V6N2P45","DOIUrl":"https://doi.org/10.5539/JPS.V6N2P45","url":null,"abstract":"This experiment was carried out to evaluate genetic stability and disease resistance in transformed soybean lines with hrpZpsta gene using PCR analysis, southern blotting, real-time quantitative PCR (qRT-PCR) and to analyze the resistance against Phytophthora sojae (P. sojae) and Cercospora sojina (C. sojina) after inoculation. The results obtained using PCR and southern blotting analytical methods showed that exogenous gene functional elements were stably inherited in transgenic soybean and hrpZpsta gene was successfully integrated into the soybean genome in a single copy. Results at high-generation (T7, T8) transgenic lines of hrpZpsta revealed that their relative expression of hrpZpsta gene was the highest in leaves followed by roots, and much lower in stems, flowers, and seeds. Activity change rates of peroxidase (POD), polyphenol oxidase (PPO) and phenylalanine ammonia lyase (PAL) showed that transgenic lines significantly enhanced receptor species. The resistance of transgenic strains T7 and T8 generations against P. sojae was significantly increased with artificial inoculation methods, and the resistance against C. sojina was increased from susceptibility to the level of resistance. Under natural conditions in the field, the response of T8 transgenic lines to C. sojina reached disease resistance level. There were no significant differences in transgenic lines and recipient variety in maturing stage, leaf shape, flower color, plant height, 100-grain weight and quality content, and the two years average yield of plots increased to 11.59% and 8.19%, which significantly higher than recipient cultivar. The current results provide data support for the release of transgenic lines.","PeriodicalId":410634,"journal":{"name":"Journal of Plant Studies","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122118517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of Callus Induction and Cell Suspension Cultures of Dendrathema Indicum var. Aromaticum a Scented Chrysanthemum","authors":"Jin Liyan, Yang Yang, W. Gao, Gong Mingxue, Wang Jijia, N. Anderson, H. Miao","doi":"10.5539/JPS.V6N2P38","DOIUrl":"https://doi.org/10.5539/JPS.V6N2P38","url":null,"abstract":"Dendranthema indicum var. aromaticum is an important aroma plant in genus Dendrathema, and the establishment of callus cultures and cell suspension cultures is the basement of further protoplast fusion studies, which make it possible to breed new fragrant chrysanthemum. In this study, the effects of different plant growth regulating substances in different concentrations on callus induction were investigated with stem segments, leaves, petioles as explants. The results showed that the optimal explants were lower stem segments according to the percentage of callus formation, callus hardness, growth potential and shoot differentiation. The optimal induction mediums were MS supplemented with 1.0 mg．l-12.4 D and 0.2 mg．l-1 6-BA. The cell suspension culture system was established by using the subculture calli. The results showed that the suitable inoculum size was 2g and the suitable cell suspension culture medium was MS supplemented with 0.2 mg．l-1 6-BA and 0.5 mg．l-1 2,4-D. The time course of cell growth showed that the greatest cell fresh weight appeared on day 14 and the highest cell viability on day 3.","PeriodicalId":410634,"journal":{"name":"Journal of Plant Studies","volume":"75 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132647680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Allometric Dynamics in Branch Growth of Crape Myrtle","authors":"Xiongwen Chen","doi":"10.5539/JPS.V6N2P28","DOIUrl":"https://doi.org/10.5539/JPS.V6N2P28","url":null,"abstract":"Tree branches provide multiple functions in tree growth. It is necessary to study the allometric patterns of branches in order to understand some quantitative perspectives in tree growth. In this study, branches of seven crape myrtle trees ( Lagerstroemia indica ) were studied to examine allometric relationships in different times. The results indicated that t he total basal area of branches at one order was far more than it at the next lower order (branches far from trunks). The scaling exponents of frequency distribution in both branch length and diameter decreased from above 1.0 in May to 0.1 in November as branches grew. The entropy of branch length and diameter both decreased at the beginning and then increased for all trees during the growing season. The observed entropy was always less than the maximum entropy. The average slenderness of branches was close to 20 for all trees. There were higher fluctuations in the slenderness within small or short branches (diameter less than 10 mm or length less than 100 cm). The scaling exponents between branch radius and length were concentrated at 1.0 for most trees. The correlation between the branch diameters of 1 st order and the number of branches at 2 nd order was not significant. The general trend and deviations in allometric relationships may help to understand the complexity in tree branch development.","PeriodicalId":410634,"journal":{"name":"Journal of Plant Studies","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131899820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}