Journal of Plant Studies最新文献_第3页

Macroscopic and Microscopic Modifications of Wood after Debarking of Trees in the South Cameroon 喀麦隆南部树木剥皮后木材的宏观和微观变化

Journal of Plant Studies Pub Date : 2021-03-20 DOI: 10.5539/JPS.V10N1P30

Ghislain Kenguem Kinjouo, M. C. M. Solefack, V. Nguetsop

{"title":"Macroscopic and Microscopic Modifications of Wood after Debarking of Trees in the South Cameroon","authors":"Ghislain Kenguem Kinjouo, M. C. M. Solefack, V. Nguetsop","doi":"10.5539/JPS.V10N1P30","DOIUrl":"https://doi.org/10.5539/JPS.V10N1P30","url":null,"abstract":"Wounding of trees by debarking has been reported to form a callus tissue. This work aims to investigate macroscopic and microscopic modifications of wood after the removal of barks in Alstonia boonei, Scorodophloeus zenkeri, and Garcinia lucida. Species that are not yet barking were wounded to conducting experimental debarking. The wound was rectangular with 30 cm vertically with a lateral extent of 10 cm. Every three months, there was a follow-up for fifteen months during which the macroscopic and microscopic phenomena were observed and recorded. Microsections of 15–20 μm thickness were taken on a piece of wood from the regenerated and intact wood, with a sledge microtome. Staining of cell walls was done with safranin and fast green to increase contrast in an anatomical slide. Analyzes of the variability of xylem anatomical features were done by semi-automatic measurements using the SpectrumSee digital image analysis software. The speed of recovery of the bark of these three species is 9.04 cm/year for A. boonei, 5.9 cm/year for S. zenkeri, and 3.85 cm/year for G. lucida. The recovery of A. boonei’s bark is the fastest, and it just takes 15 months to heal its wound. Densities of vessels were 8, 38, and 17 per mm2, respectively, for the wood of A. boonei, S. zenkeri, and G. lucida before barking. These values increased for A. boonei (26) and G. lucida (20) except for S. zenkeri (25). In all species, the diameter of the vessels has decreased in the regenerated wood. Management practices that enhance the monitoring of sustainable harvesting levels of species and promote alternative plants for the same uses should be considered as part of conservation strategies.","PeriodicalId":410634,"journal":{"name":"Journal of Plant Studies","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121373807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Post-harvest Loss Assessment of Banana (Musa spp.) at Jimma Town Market 吉马镇市场香蕉(Musa)收获后损失评估

Journal of Plant Studies Pub Date : 2021-03-10 DOI: 10.5539/JPS.V10N1P23

G. Gemechu, Damtew Abewoy, Kedir Jaleto

{"title":"Post-harvest Loss Assessment of Banana (Musa spp.) at Jimma Town Market","authors":"G. Gemechu, Damtew Abewoy, Kedir Jaleto","doi":"10.5539/JPS.V10N1P23","DOIUrl":"https://doi.org/10.5539/JPS.V10N1P23","url":null,"abstract":"Post-harvest loss of banana in Jimma town market was accounted a total loss of 26.5% in the supply chain. Of these, more percent of the total losses were being observed at the retail market (64.10%) and whole-salers level (35.90%). Mechanical damage followed by improper transport and improper storage were identified as the main causes of banana loss at whole-salers level while fruit rotting followed by improper ripening and mechanical damage were identified as the main causes to the loss of banana fruit at retail level. Hence, the current post-harvest management system of banana at whole-salers and retail level is inadequate. There is no sufficient attention given for the post-harvest management of banana in the supply chain. It was also observed that, there is a knowledge gap between the respondents in their experience of proper fruit handling techniques. Therefore, to reduce the level of post-harvest losses of banana, more emphasis should be given to post-harvest handling practices. The loss can be minimized or prevented by awareness creation, education and training about the importance of post-harvest losses, adopting better management operations, careful handling and packaging to the supply chain actors.","PeriodicalId":410634,"journal":{"name":"Journal of Plant Studies","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133609497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Antifungal Effects of Paraquat and Glyphosate on Rhizoctonia solani (Kühn) in Potato in vitro Condition 百草枯和草甘膦对马铃薯根瘤菌k<e:1>的体外抑菌作用

Journal of Plant Studies Pub Date : 2021-01-04 DOI: 10.5539/jps.v10n1p16

J. Vázquez, A. S. Arizpe, M. Cepeda, C. Ramos

{"title":"Antifungal Effects of Paraquat and Glyphosate on Rhizoctonia solani (Kühn) in Potato in vitro Condition","authors":"J. Vázquez, A. S. Arizpe, M. Cepeda, C. Ramos","doi":"10.5539/jps.v10n1p16","DOIUrl":"https://doi.org/10.5539/jps.v10n1p16","url":null,"abstract":"Potato is one of the main crops worldwide. It this research, antifungal activity in vitro of paraquat and glyphosate were evaluated for Rhizoctonia solani control. R. solani was identified from potato tubers collected out from at open markets in Saltillo, Coahuila, Mexico. Two types of herbicides were applied: paraquat and glyphosate, at four different dosage treatments of: 10, 100, 1 000 and 10 000 μL. One 5 mm diameter PDA disc with R. solani mycelium was placed at the center of the Petri dish, with a radial registry fungal every 24 h for 192 h. Pathogen was identified by morphological criteria and the data was evaluated randomly with a factorial arrangement, on which, herbicides represented factor A and dosage treatments were represented by factor B. Thus experimental design had two levels for factor A and five levels for factor B with six replications. The results were analyzed by the SAS version 9.1 statistical program, the mean separation with the Tukey test (p=0.05). Glyphosate achieved inhibition of R. solani by 35.5882% and paraquat up to 80.0399%. Results reveal the importance of the need for more studies of these herbicides as fungicides. High concentrations of paraquat (10 000 μL) inhibits R. solani, and glyphosate does not affect R. solani mycelium development at low dosages (10 and 100 μL) and inhibits it at higher doses (10 000 μL).","PeriodicalId":410634,"journal":{"name":"Journal of Plant Studies","volume":"116 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130215266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effect of Thinning on Pericopsis elata (Harms) Meeuwen (Fabaceae) Found in Forest Plantations in the East and South Regions of Cameroon 间伐对喀麦隆东部和南部森林人工林中elata (Harms) Meeuwen (Fabaceae)的影响

Journal of Plant Studies Pub Date : 2020-12-20 DOI: 10.5539/jps.v10n1p1

J. Betti, Ferdinand Kemkeng, J. Ngueguim, J. Ambara, M. Tchatat

引用次数: 2

Reviewer Acknowledgements for Journal of Plant Studies, Vol. 9, No. 2 《Journal of Plant Studies》第9卷第2期审稿人致谢

Journal of Plant Studies Pub Date : 2020-08-30 DOI: 10.5539/jps.v9n2p46

Joan Lee

{"title":"Reviewer Acknowledgements for Journal of Plant Studies, Vol. 9, No. 2","authors":"Joan Lee","doi":"10.5539/jps.v9n2p46","DOIUrl":"https://doi.org/10.5539/jps.v9n2p46","url":null,"abstract":"Journal of Plant Studies wishes to acknowledge the following individuals for their assistance with peer review of manuscripts for this issue. Their help and contributions in maintaining the quality of the journal are greatly appreciated. \u0000Journal of Plant Studies is recruiting reviewers for the journal. If you are interested in becoming a reviewer, we welcome you to join us. Please contact us for the application form at: jps@ccsenet.org \u0000Reviewers for Volume 9, Number 2 \u0000Adriana F. Sestras, University of Agricultural Sciences and Veterinary Medicine, Romania \u0000Alejandra Matiz, University of Sao Paulo, Brazil \u0000Fardausi Akhter, Agriculture and Agri-Food Canada, Canada \u0000Khyati Hitesh Shah, Stanford University, United States \u0000Kirandeep Kaur Mani, California seed and Plant Labs, USA \u0000Malgorzata Pietrowska-Borek, Poznan University of Life Sciences, Poland \u0000Massimo Zacchini, National Research Council of Italy, Italy \u0000Md. Asaduzzaman, Agricultural Research Institute, Bangladesh \u0000Melekber Sulusoglu, Arslanbey Vocational School Kocaeli University, Turkey \u0000Milana Trifunovic-Momcilov, Institute for Biological Research “Sinisa Stankovic”, Serbia \u0000Mohamed Ahmed El-Esawi, Tanta University, Egypt \u0000Rakesh Ponnala, Zoetis Inc, United States \u0000Romina A. Marc, Univ. of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Romania \u0000Slawomir Borek, Adam Mickiewicz University, Poland \u0000Tomoo misawa, Donan Agricultural Experiment Station, Hokkaido Research Organization, Japan","PeriodicalId":410634,"journal":{"name":"Journal of Plant Studies","volume":"66 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124033679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro Antioxidant Activity of Mangifera indica Leaf Extracts 芒果叶提取物的体外抗氧化活性研究

Journal of Plant Studies Pub Date : 2020-06-26 DOI: 10.5539/jps.v9n2p39

Kimihisa Itoh, T. Matsukawa, M. Okamoto, Kanasa Minami, N. Tomohiro, Kosuke Shimizu, S. Kajiyama, Yuichi Endo, H. Matsuda, S. Shigeoka

{"title":"In vitro Antioxidant Activity of Mangifera indica Leaf Extracts","authors":"Kimihisa Itoh, T. Matsukawa, M. Okamoto, Kanasa Minami, N. Tomohiro, Kosuke Shimizu, S. Kajiyama, Yuichi Endo, H. Matsuda, S. Shigeoka","doi":"10.5539/jps.v9n2p39","DOIUrl":"https://doi.org/10.5539/jps.v9n2p39","url":null,"abstract":"In this study, we aimed to identify the utility of pruned mango (Mangifera indica ‘Irwin’) leaves as a resource for ingredients with antioxidant activity. Firstly, we examined the antioxidant activity of extracts obtained from the pericarps, flesh, flowers, barks, seeds, young dark reddish brown leaves (YDL-ext), young yellow leaves (YYL-ext), and pruned old dark green leaves (OML-ext) obtained from ‘Irwin’ mango. Among them, methanolic extract of flower and OML-ext showed the most potent 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and superoxide dismutase (SOD)-like activity. The flesh extract showed weak DPPH radical scavenging activity, but did not show SOD-like activity. Secondly, we investigated the relationship between the maturation of leaves and their antioxidant activity by considering the contents of their two active polyphenolic components, 3-C-β-D-glucosyl-2,4,4’,6-tetrahydroxybenzophenone (1) and mangiferin (2), in addition to chlorophyll (3) and anthocyanins represented by cyanidin-3-O-glucoside (4). The DPPH radical scavenging activity of YDL-ext, YYL-ext and OML-ext were mainly attributable to 1, 2 and 3, whereas their SOD-like activity was partly attributable to 2. The DPPH radical scavenging and SOD-like activities of YDL-ext and YYL-ext were attributable to 1 and 2. These activities were also due to anthocyanins whose content is highest in YDL-ext. Considering the amounts of leaves obtained from pruning, old dark green leaves may be a reasonable natural resource for preparing cosmetics and/or supplemental ingredients with health-enhancing properties, antioxidant activity and inhibitory effect on AGEs formation and pancreatic lipase.","PeriodicalId":410634,"journal":{"name":"Journal of Plant Studies","volume":"63 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114692743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Anatomical Characteristics of Sunlight-induced Bark (Periderm) Coverages on Columnar Cacti of Central Mexico 墨西哥中部柱状仙人掌光照诱导树皮(表皮)覆盖度的解剖学特征

Journal of Plant Studies Pub Date : 2020-05-26 DOI: 10.5539/jps.v9n2p20

L. Evans, Phillip Dombrovskiy

{"title":"Anatomical Characteristics of Sunlight-induced Bark (Periderm) Coverages on Columnar Cacti of Central Mexico","authors":"L. Evans, Phillip Dombrovskiy","doi":"10.5539/jps.v9n2p20","DOIUrl":"https://doi.org/10.5539/jps.v9n2p20","url":null,"abstract":"More than twenty-three species of tall, long-lived columnar cacti from a large variety of locations within the Americas show sunlight-induced periderm development on their stems. Periderm coverages lead to cactus morbidity and mortality. Our objective was to determine if periderm coverage patterns and anatomical characteristics of periderm formation differ among five cactus species located at a single site. Periderm coverages, patterns of periderm coverages and histological changes during the periderm formation process were determined for five native species of tall, long-lived columnar cacti in the Tehuacan Valley of Puebla, Mexico during May to June 2019. Periderm coverages and patterns of periderm on cactus surface varied among the species. On surfaces, some species had periderm form at crests initially, while one species had initial periderm form where troughs join. All species had the same internal tissues but the characteristics of these tissues varied among species. In response to periderm formation, one species retained its cuticle while one species retained its hypodermis intact and another produced cork cells inside the hypodermis. Overall, the histological changes that result from periderm formation were specific for each species and no pair of species showed the same responses to periderm formation. In conjunction with data from species from South America, eight distinct scenarios of histological manifestations were documented. Although, each of the five cactus species were in the same location and received the same amount of sunlight exposures, each species showed unique periderm coverages on surfaces, unique anatomical characteristics and unique anatomical responses. Thus, location was not the primary determinant of responses.","PeriodicalId":410634,"journal":{"name":"Journal of Plant Studies","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128904859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Inhibitory Effect of Several Mangifera indica Cultivar Leaf Extracts on the Formation of Advanced Glycation End Products (AGEs) 几种芒果品种叶片提取物对晚期糖基化终产物(AGEs)形成的抑制作用

Journal of Plant Studies Pub Date : 2020-05-26 DOI: 10.5539/jps.v9n2p33

Kimihisa Itoh, T. Matsukawa, Kanasa Minami, M. Okamoto, N. Tomohiro, Kosuke Shimizu, S. Kajiyama, Yuichi Endo, H. Matsuda, S. Shigeoka

{"title":"Inhibitory Effect of Several Mangifera indica Cultivar Leaf Extracts on the Formation of Advanced Glycation End Products (AGEs)","authors":"Kimihisa Itoh, T. Matsukawa, Kanasa Minami, M. Okamoto, N. Tomohiro, Kosuke Shimizu, S. Kajiyama, Yuichi Endo, H. Matsuda, S. Shigeoka","doi":"10.5539/jps.v9n2p33","DOIUrl":"https://doi.org/10.5539/jps.v9n2p33","url":null,"abstract":"As a part of our ongoing research to find novel functions in mango leaves, we have reported that the methanolic extract of pruned old dark green mango leaf (Mangifera indica ‘Irwin’) exhibited inhibitory effects on the formation of advanced glycation end products (AGEs) in nonenzymatic glycation of albumin. The purpose of this study was to find other mango cultivars with more potent activity in this regard. We examined the inhibitory effect of seventeen mango (Mangifera indica) cultivar leaf extracts on AGEs formation. We also investigated the relationship between the inhibitory activity of the extracts and the contents of their active components, 3-C-β-D-glucosyl-2,4,4’,6-tetrahydroxybenzophenone (1), mangiferin (2) and chlorophyll (3). On the basis of the evaluation of the inhibitory activity of mango cultivar leaf extracts, the HPLC determination of the contents of 1 and 2, and the spectrophotometric determination of 3, it was found that almost all extract showed a significant activity, and the content of 2 and 3 detected in each was similar. In contrast, AGEs formation inhibition tended to be higher as the content of 1 in the leaf extracts increased. This is the first report of phytochemical analysis of compounds 1, 2 and 3 in various cultivars of mango leaf. From the phytochemical point of view, these results suggest that the pruned leaves of any cultivar of Mangifera indica except ‘Chiin Hwang No. 1’ and ‘Kyo Savoy’ may be useful for the preparation of natural ingredients with inhibitory activity of AGEs formation.","PeriodicalId":410634,"journal":{"name":"Journal of Plant Studies","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130067044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Pyrethrum (Chrysanthemum cinerariifolium) Flowers’ Drying Conditions for Optimum Extractable Pyrethrins Content 除虫菊(菊)花干燥条件对提取除虫菊酯含量的影响

Journal of Plant Studies Pub Date : 2020-05-13 DOI: 10.5539/jps.v9n2p11

H. O. Otieno, D. Kariuki, J. Wanjohi

{"title":"Pyrethrum (Chrysanthemum cinerariifolium) Flowers’ Drying Conditions for Optimum Extractable Pyrethrins Content","authors":"H. O. Otieno, D. Kariuki, J. Wanjohi","doi":"10.5539/jps.v9n2p11","DOIUrl":"https://doi.org/10.5539/jps.v9n2p11","url":null,"abstract":"Pyrethrum flowers of the genus Chrysanthemum cinerariifolium are grown in Kenya by small scale farmers for extraction of pyrethrins, a natural insecticide’s active ingredient. Pyrethrins are classified in two groups, Pyrethrins I and II, and are degradable when exposed to air, moisture and high temperatures. The contents and ratio of Pyrethrins I:II determine the efficacy of the insecticide. Therefore, drying of the pyrethrum flowers should be optimized in order to attain maximum extractable pyrethrins content and optimum ratio. The aim of this research was to optimize the drying temperatures, time and moisture content of pyrethrum flowers. The flowers were harvested and dried at varying temperatures of 40, 50, 60 and 70 oC to total dryness. Another set of flowers were harvested and dried in the oven at the same temperatures for a maximum period of 18 hrs. Moisture content was determined at each temperature, at intervals of one hour. The dried flowers were then ground into fine powder and extracted using Soxhlet extraction method with hexane. The extracts were refined and analyzed by Mercury reduction and High Performance Liquid Chromatographic methods. Pyrethrum flowers were found to achieve maximum moisture loss, at varying times and temperature with 70 oC recording the shortest time of 18 hrs. The yield of pyrethrins obtained on drying the flowers to constant weight at 40 oC was 0.90% while drying for 18 hrs yielded 0.79%. Extractable Pyrethrins II were found to reduce by 8.6% when the drying temperature was raised from 50 to 60 oC and by 11.3% from 60 to 70 oC. Extractable Pyrethrins I were found to reduce by 6% when the drying temperature was raised from 50 to 60 oC and by 5% from 60 to 70 oC. The total pyrethrins obtained from the flowers dried at 50 oC were found to be 1.37% at 18 hrs and 1.44% to constant weight drying. The pyrethrins I:II ratio was found to vary over the temperature range 40-70OC.The optimum temperature and time for drying pyrethrum flowers was found to be 50 oC for 21 hrs.","PeriodicalId":410634,"journal":{"name":"Journal of Plant Studies","volume":"278 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115928241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

New Factors for Protoplast-Callose-Fiber Formation in Salt-Tolerant Mangrove Plants, Avicennia alba and Bruguiera sexangula and Analysis of Fiber Substructures 红树耐盐原生质体-胼胝质纤维形成的新因素及纤维亚结构分析

Journal of Plant Studies Pub Date : 2020-05-02 DOI: 10.5539/jps.v9n2p1

Asami Kurita-Tashiro, N. Hayashi, Tomoya Oyanagi, H. Sasamoto

{"title":"New Factors for Protoplast-Callose-Fiber Formation in Salt-Tolerant Mangrove Plants, Avicennia alba and Bruguiera sexangula and Analysis of Fiber Substructures","authors":"Asami Kurita-Tashiro, N. Hayashi, Tomoya Oyanagi, H. Sasamoto","doi":"10.5539/jps.v9n2p1","DOIUrl":"https://doi.org/10.5539/jps.v9n2p1","url":null,"abstract":"Elongated and spiral β-1,3-glucan (callose) fibers were obtained by new factors from protoplasts cultured in liquid medium from suspension cultured cells of two salt-tolerant mangrove species; Avicennia alba and Bruguiera sexangula. Differences in salt factor for protoplast-fiber formation were compared with those of the callose fibers developed from protoplasts of non-mangrove tree plants, Larix leptolepis and Betula platyphylla, which high concentrations of divalent cations, Mg2+ (50 mM) or Ca2+ (100 mM), were stimulatory. In the halophilic A. alba protoplasts, whose cell division was stimulated by up to 400 mM NaCl, addition of Mg2+, Ca2+, K+ ions inhibited protoplast-fiber formation. In B. sexangula, protoplast-fibers were rapidly and efficiently formed only by another new factor, electric cell fusion treatment of protoplasts. Spiral fibers developed from mangrove protoplasts were detected under an inverted microscope, and their specific blue-green color for callose after staining with Aniline Blue dye was detected under a fluorescence microscope. Enzymatic certification of callose was further performed with laminarinase, specific for callose, in comparison with cellulase CBH1, specific for cellulose. Differences in sub-structures, fibrils and sub-fibrils of two mangrove protoplast-fibers were analyzed using laser confocal scanning microscopy, atomic force microscopy and image J analysis. Tube-like fine structure was observed using transmission electron microscopy in single protoplast-fiber of B. sexangula selected with a micromanipulator.","PeriodicalId":410634,"journal":{"name":"Journal of Plant Studies","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116002524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2