{"title":"Alpha-, gamma- and beta-proteobacteria detected in legume nodules in Latvia, using full-length 16S rRNA gene sequencing","authors":"A. Klūga, L. Dubova, I. Alsina, N. Rostoks","doi":"10.1080/09064710.2023.2232681","DOIUrl":"https://doi.org/10.1080/09064710.2023.2232681","url":null,"abstract":"ABSTRACT\u0000 Rhizobia are soil bacteria that form nodules on legumes and fix atmospheric nitrogen, reducing the need for chemical fertilisers. In most studies rhizobia are described as alpha-proteobacteria and have been studied extensively for several decades. However, in recent years an increasing number of studies present beta- and gamma-proteobacteria strains as potential rhizobia-like bacteria. In this study we identify bacteria isolated from nodules of various legumes grown in Latvia, using a full-length 16S rRNA gene sequence, used for more precision, compared with a partial sequence of the gene. Bacterial strains in this study have been isolated from 1962–2019, part of which are rhizobia strains from a unique historical collection. This is the first time bacterial strains isolated from nodules in Latvian soils have been identified with molecular biology methods. A wide taxonomic diversity was detected – bacteria species from α-, β-, γ-proteobacteria classes and Paenibacillus polymyxa strains from the Bacilli class. P. polymyxa strains were detected only in the historical collection, while β- and γ-proteobacteria strains were obtained only from the newly isolated specimens, uncovering new potential strains for a commercial legume seed inoculum. Soil samples were collected, and phosphorus levels were determined. Several strains indicate phosphate solubilising properties.","PeriodicalId":40817,"journal":{"name":"Acta Agriculturae Scandinavica Section B-Soil and Plant Science","volume":"21 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83562251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ana Flávia Bastos Ongaro, Janaina Azevedo Martuscello, Flavia Maria de Andrade Gimenes, Ana Carolina Lopes Batista, Lucas Ferreira Penteado, L. M. Premazzi, Waldssimiler Teixeira de Mattos, Marcelo Moretin Vieira, Ricardo Lopes Dias da Costa, L. Gerdes
{"title":"Canopy height impact on legume mass and Megathyrsus maximus tiller dynamics in mixed pastures","authors":"Ana Flávia Bastos Ongaro, Janaina Azevedo Martuscello, Flavia Maria de Andrade Gimenes, Ana Carolina Lopes Batista, Lucas Ferreira Penteado, L. M. Premazzi, Waldssimiler Teixeira de Mattos, Marcelo Moretin Vieira, Ricardo Lopes Dias da Costa, L. Gerdes","doi":"10.1080/09064710.2023.2226650","DOIUrl":"https://doi.org/10.1080/09064710.2023.2226650","url":null,"abstract":"ABSTRACT The use of grazing management strategies can influence canopy structure and the permanence of legumes. The objective of this work was to evaluate the determination of forage canopy heights as a grazing management strategy regarding herbage mass composition and tiller population dynamics in multispecies pastures comprising Megathyrsus maximus cv. Aruana and the legumes Macrotyloma axillare, Calopogonium mucunoides and Stylosanthes spp. Four treatments, defined by canopy heights of 15, 30, 45 and 60 cm, were evaluated in a randomised block design with four replications. The evaluations were performed under continuous stocking with sheep. Taller pastures (45 and 60 cm) contained greater (P < 0.05) total herbage mass of Aruana leaves and stems and also higher mass of legumes than smaller pastures (15 and 30 cm). The grass tiller population density was different between heights (P < 0.05) of 2.71, 2.02, 1.97 and 1.47 tillers/m2 for heights of 15, 30, 45 and 60 cm, respectively. The tiller appearance rate was highest in tall pastures (P < 0.05) and the lowest rate and stability index were found in 15 cm pastures. Aruana guineagrass and forage legumes can be managed in the height range of 30–60 cm under continuous stocking. GRAPHICAL ABSTRACT","PeriodicalId":40817,"journal":{"name":"Acta Agriculturae Scandinavica Section B-Soil and Plant Science","volume":"12 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2023-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87228389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Sandy silt loam soil may hamper the inoculation effect on lucerne (Medicago sativa L.) growth","authors":"I. Stūrīte, T. Lunnan, L. Østrem","doi":"10.1080/09064710.2023.2212674","DOIUrl":"https://doi.org/10.1080/09064710.2023.2212674","url":null,"abstract":"ABSTRACT Despite newly approved lucerne cultivars, this has not led to increased use of this legume in high-latitude agriculture. Challenges with inoculation by Rhizobium meliloti have been identified as a bottleneck to adaptation. Here we tested inoculation sources (ISs) with soil types and cultivars in pot and field experiments. During a one-year outdoor pot experiment, we tested the impact of IS (wet peat slurry and Nitragin Gold dry inoculation) and three soil types (sand, sandy silt and /peat soil) on nodule development, shoot and root growth and winter survival of one hybrid lucerne cultivar (‘Ludvig’). The pot experiment revealed that dry inoculation led to significant better plant growth, flower and nodule development as well as plant regrowth after winter survival. Peat soil appeared as the best growth medium and silty soil limited inoculation efficiencies. In field trials at two locations differing in soil characteristics using similar ISs, and three hybrid lucerne cultivars (‘Lavo’, ‘Live’ and ‘Lotte’) biomass yield during two ley years showed site as well as cultivar differences. Such environmental interactions in the field trials justify the use of adapted cultivars, and dry inoculation should be recommended for practical use replacing peat slurry inoculation.","PeriodicalId":40817,"journal":{"name":"Acta Agriculturae Scandinavica Section B-Soil and Plant Science","volume":"3 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91196962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Ogola, Vhulenda Madzivhandila, S. Maseko, Terry M. Leboho
{"title":"P-fertiliser and rhizobial inoculation increased the concentration of mineral nutrients in the rhizosphere of two chickpea genotypes","authors":"J. Ogola, Vhulenda Madzivhandila, S. Maseko, Terry M. Leboho","doi":"10.1080/09064710.2023.2197457","DOIUrl":"https://doi.org/10.1080/09064710.2023.2197457","url":null,"abstract":"ABSTRACT Co-application of fertilisers and biofertilisers increases solubility and concentration of nutrient elements in the soil. However, information on the effect of P fertiliser and rhizobium inoculation on the concentration of mineral elements in the rhizosphere of field-grown chickpea in contrasting soil types is lacking. We assessed the effect of phosphorus fertiliser, rhizobium inoculation, and rhizobium inoculation plus P fertilizer on the concentration of nutrients in the rhizosphere of 2 chickpea genotypes (ACC#1 & ACC#5) at clay and sandy soil sites in two consecutive years. ACC#5 accumulated higher amounts of rhizospheric nutrients than ACC#1 in both soil types despite the variable response to fertiliser treatment. Rhizobium inoculation plus P fertiliser resulted in higher accumulations of rhizosphere N (7-219%), P (20-348%), K (9-365%), Ca (2-155%) and Zn (15-259%) compared to the other fertiliser treatments suggesting an additive effect of P fertiliser and rhizobium inoculation. Moreover, the effect of fertiliser treatments was more pronounced in the clay than the sandy soil. Our investigation is the first to document the effects of fertiliser treatment, genotype, and soil type on the accumulation of rhizosphere nutrients in field-grown chickpea. However, we propose further studies, incorporating more cultivars and environments, before making practical recommendations.","PeriodicalId":40817,"journal":{"name":"Acta Agriculturae Scandinavica Section B-Soil and Plant Science","volume":"23 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89504589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. A. Masenya, Thembelihle Ellain Mdluli, Setshele Standford Thosago, Z. Dube, Mmagadima Lauraine Sebati
{"title":"Host status and host sensitivity of Kickapoo white tepary bean to Meloidogyne entorolobii","authors":"T. A. Masenya, Thembelihle Ellain Mdluli, Setshele Standford Thosago, Z. Dube, Mmagadima Lauraine Sebati","doi":"10.1080/09064710.2023.2195866","DOIUrl":"https://doi.org/10.1080/09064710.2023.2195866","url":null,"abstract":"ABSTRACT Tepary bean (Phaseolus acutifolius A. Gray) is a drought tolerant leguminous crop and due to its multi-purpose use, it can easily be incorporated into different production systems. It being an undertilised crop, its production is usually relegated to poor sandy soils that are highly prone to root-knot nematodes (Meloidogyne species). Commonly communal producers of tepary beans use retained seeds with no knowledge of the nematode resistance status of the seeds. Hence the objective of the study was to determine whether Meloidogyne enterolobii will be able to reproduce on the Kickapoo white tepary bean and cause a reduction in the plant's growth variables. To achieve this objective, Kickapoo white tepary bean seedlings were exposed to 0, 25, 50, 125, 250, 625, 1250 and 3125 M. enterolobii eggs and second-stage juveniles (J2) in 2021 and validated in 2022 under shade-net conditions. At 56 days after inoculation, plant and nematode variables were collected and reproductive factor (Rf) was computed. Plant growth variables were not reduced whereas, nematode variables increased with an increase in nematode levels. In both experiments the Rf values were above a unity for all inoculation levels, indicating that nematodes were able to reproduce.. In conclusion, Kickapoo white tepary bean is tolerant to M. enterolobii.","PeriodicalId":40817,"journal":{"name":"Acta Agriculturae Scandinavica Section B-Soil and Plant Science","volume":"23 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2023-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78524442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. M. Abdullah, Amir Khan, Hasan B. Albargi, M. Ahmad, J. Ahmad, Faheem Ahmad, Mohammad Shabib Akhtar, Nehal Mohsin, Fuzail Ahmad, M. A. Kamal, Yaser E. Alqurashi, H. Lal, JariS. Algethami
{"title":"Ipomoea carnea associated phytochemicals and their in silico investigation towards Meloidogyne incognita","authors":"M. M. Abdullah, Amir Khan, Hasan B. Albargi, M. Ahmad, J. Ahmad, Faheem Ahmad, Mohammad Shabib Akhtar, Nehal Mohsin, Fuzail Ahmad, M. A. Kamal, Yaser E. Alqurashi, H. Lal, JariS. Algethami","doi":"10.1080/09064710.2023.2194305","DOIUrl":"https://doi.org/10.1080/09064710.2023.2194305","url":null,"abstract":"ABSTRACT Root-knot nematodes (Meloidogyne spp.) are sedentary endo-parasite that causes severe yield loss in carrot. Chemical nematicides currently used to manage Meloidogyne incognita are being phased out because of rising health and environmental issues. This study aimed to evaluate nematicidal effect of various concentrations, viz., 250, 500, 750, 1000ppm of leaf extract of Ipomoea carnea against M. incognita infecting carrot under in vitro and in pots assays. In our result, all tested concentrations displayed J2s mortality and egg hatching inhibition along with improving growth of carrot and reduced J2s population and root-knot index. Molecular docking performed predicts binding interactions of two major compounds, viz., neophytadiene and 2-amino-2-methyl-1-propanol as shown by GC-MS analysis with targeted protein, odorant response gene-1 of M. incognita, to confirm nematicidal action of I. carnea leaf extract. The obtained results also suggested that neophytadiene interacted more and strongly bound with odorant response gene-3 than 2-amino-2-methyl-1-propanol. The biochemical ligand-target protein interaction described in the present work will be helpful in the logical selection of biomolecules and essential proteins. Therefore, plant extract may be used the best alternative to chemical nematicides to control root-knot nematodes and caused longitudinal growth of the plant as well as reduce environmental risks.","PeriodicalId":40817,"journal":{"name":"Acta Agriculturae Scandinavica Section B-Soil and Plant Science","volume":"27 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2023-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77079886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of long-term fertilisation of Calcisols on organic carbon sequestration in marine climate in Estonia","authors":"V. Loide","doi":"10.1080/09064710.2023.2187317","DOIUrl":"https://doi.org/10.1080/09064710.2023.2187317","url":null,"abstract":"ABSTRACT The aim of this work was to investigate the effect of long-term (45-year) fertilization of the crop rotation on soil organic carbon concentration (SOC%) changes in different periods of the experiment and its trend under conditions of more northerly marine climate changes. In this work, the data of the long-term NPK fertilization experiment of sandy loam Calcisols located in Northern Estonia (longitude 58.584816, latitude 24.422128) have been used. The crop rotation was divided into six blocks: potato-spring barley-spring barley under sowing with forage grasses – 1y and 2y forage grasses-winter rye. Different combinations of mineral (N 40—180; P 18—54; K 50—105 kg ha−1) and organic (manure (FYM) 30 and 60 Mg ha−1) fertilizers were used in the study. Two periods were observed: the last 20y vegetation period was 10% warmer and 15% less rainy than the earlier period. Under conditions of climate warming, the yield of barley and potatoes increased the most, especially with manure application. SOC% increased from 2.01 to 2.04 under the influence of mineral fertilizers, and from 2.08 to 2.22 with mineral + FYM. The soil is sustainable if manure is used at least 5–10 Mg ha−1 per y−1.","PeriodicalId":40817,"journal":{"name":"Acta Agriculturae Scandinavica Section B-Soil and Plant Science","volume":"62 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2023-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76853054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Genetu Fekadu, E. Adgo, D. Meshesha, A. Tsunekawa, Nigussie Haregeweyn, F. Peng, M. Tsubo, Asaminew Tassew, G. Tiruneh, N. Tadesse, T. Masunaga
{"title":"Dry-season autotrophic and heterotrophic soil respiration in contrasting agroecological settings","authors":"Genetu Fekadu, E. Adgo, D. Meshesha, A. Tsunekawa, Nigussie Haregeweyn, F. Peng, M. Tsubo, Asaminew Tassew, G. Tiruneh, N. Tadesse, T. Masunaga","doi":"10.1080/09064710.2023.2189613","DOIUrl":"https://doi.org/10.1080/09064710.2023.2189613","url":null,"abstract":"ABSTRACT In arid Africa, it is unclear how the transition from grassland to cropland affects CO2 fluxes and whether these effects are climate-dependent. From November 2019 to March 2020 (dry season), we studied autotrophic (Ra) and heterotrophic (Rh) soil respiration. At highlands, grazing land (1.18 ± 0.13 µmol m−2 s−1) and teff croplands (1.36 ± 0.14 µmol m−2 s−1) had the lowest Rh, while acacia decurrens plantations (1.94 ± 0.19 µmol m−2 s−1) were the largest. When compared to teff (2.08 ± 0.20 µmol m−2 s−1) and khat (1.73 ± 0.24 µmol m−2 s−1), grazing land in the midland had a lower Rh (1.18 ± 0.13 µmol m−2 s−1). Ra on teff (1.51 ± 0.27 µmol m−2 s−1) was substantially (P < 0.05) greater than on grazing land (1.08 ± 0.32 µmol m−2 s−1) in the highlands. Grazing lands (1.54 ± 0.21 µmol m−2 s−1) have a considerably (P < 0.05) greater Ra than farmlands (teff, 0.20 ± 0.07 µmol m−2 s−1; groundnut, 0.09 ± 0.03 µmol m−2 s−1) in lowlands. Growing teff in the highlands and midlands will result in higher CO2 fluxes into the atmosphere. However, in-depth research is needed to implement effective land management that reduces CO2 emissions.","PeriodicalId":40817,"journal":{"name":"Acta Agriculturae Scandinavica Section B-Soil and Plant Science","volume":"101 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2023-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88964355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mengen Nie, Na Ning, Du Liang, Haiping Zhang, Shuangshuang Li, Shuai Li, Xinqi Fan, Yizhong Zhang
{"title":"Seed priming with selenite enhances germination and seedling growth of Sorghum [Sorghum bicolor (L.) Moench] under salt stress","authors":"Mengen Nie, Na Ning, Du Liang, Haiping Zhang, Shuangshuang Li, Shuai Li, Xinqi Fan, Yizhong Zhang","doi":"10.1080/09064710.2023.2177561","DOIUrl":"https://doi.org/10.1080/09064710.2023.2177561","url":null,"abstract":"ABSTRACT Justify that the use of selenium has been shown to promote seed germination and seedling growth of some crops under salt stress but the physiological mechanisms in sorghum remain poorly understood. Herein, grain sorghum [Sorghum bicolor (L.) Moench] variety Jinza 2002 was used to explore the effects and mechanisms of seed priming with 25 μmol/L sodium selenite [Se(IV)] on seed germination, seedling growth, antioxidant enzyme activity and osmotic regulatory substances under 120 mmol/L NaCl. The results showed that soaking sorghum seeds in Se(IV) effectively alleviated the harm of salt stress. Compared with the Na group, Se(IV) application increased germination percentage (by 23.8%), while facilitating the elongation of radicles (by 4.2%) and plumules (by 18.4%) under salt stress. Salt-stressed seedlings showed increased levels of leaf chlorophyll, soluble sugar, soluble protein and proline contents, and antioxidant enzyme activities, as well as root activity, and total biomass following Se(IV) application, along with decreased relative electrical conductivity and malondialdehyde content in the leaves. Findings of this study indicate that seed priming with Se(IV) facilitates sorghum seed germination and seedling growth under salt stress by improving root activity, enhancing leaf photosynthesis and antioxidant responses, and reducing cell membrane damage.","PeriodicalId":40817,"journal":{"name":"Acta Agriculturae Scandinavica Section B-Soil and Plant Science","volume":"37 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2023-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76838078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fannie Zhang, J. Qi, C. Gui, Yilin Zhang, Zheng Wang
{"title":"The lower labile carbon of surface soils in Chinese semiarid areas","authors":"Fannie Zhang, J. Qi, C. Gui, Yilin Zhang, Zheng Wang","doi":"10.1080/09064710.2023.2174044","DOIUrl":"https://doi.org/10.1080/09064710.2023.2174044","url":null,"abstract":"ABSTRACT\u0000 Hot water extractable organic carbon (HWOC), the labile carbon component, is often used to indicate soil organic carbon (SOC) dynamics. Nevertheless, few studies have been carried out in arid climate areas which affects our full understanding of HWOC. Here, we investigated the change in HWOC in the topsoil of different ecosystems in the southern part of the Loess Plateau in the semiarid region of China and compared it with that in other regions. The HWOC concentrations of the study area (0-10 cm) were 0.27 ± 0.12 g C kg−1 and 0.19 ± 0.04 g C kg−1 in the natural and agricultural systems respectively, and the HWOC proportions were 1.38 ± 0.38% and 2.18 ± 0.22%. The HWOC concentration and proportion in the study area were much lower than the reported data in other areas, which may be affected by drought conditions. Irrigation could weaken the difference in HWOC between agricultural systems in different regions. Since HWOC is easily lost due to the impact of the arid climate, the soil carbon balance and carbon sequestration in arid and semiarid areas are relatively unstable, indicating that soil management should be improved in combination with water management.","PeriodicalId":40817,"journal":{"name":"Acta Agriculturae Scandinavica Section B-Soil and Plant Science","volume":"10 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2023-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87044270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}