{"title":"Development of Disease Scoring System for Severity Analysis of Late Blight of Potato based on Image Processing Approach","authors":"S. Mandal","doi":"10.31031/cjmi.2021.05.000601","DOIUrl":"https://doi.org/10.31031/cjmi.2021.05.000601","url":null,"abstract":"The severity for late blight disease of potato is quantified based on standard disease grading scale mainly by measure with the eye, which is greatly influenced by subjective factors and results in obvious error using Henfling disease estimation scale. The plant pathologists generally graded the late blight disease severity based on eye estimation for percent infected area according to the disease grading scale of Henfling. The disease scoring system is primarily the eye estimation methods and scores are varied among different pathologists. In this paper, an attempt has been made to develop image processing-based disease estimation for late blight of potato. The late blight affected portions of leaflets, leaves and whole plants have been evaluated using image analysis system. The percentages of affected areas have been calculated and scores have been assigned based on proposed scale. The assigned scores have also been verified from several plant pathologists based on Henfling’s disease scale. The gap of disease intensity variation between pathologist’s evaluation and image processing system has been fine-tuned by modification of the scale and finally the accuracy of scores estimation based on proposed image analysis system is 85% and could be effectively exploited for late blight of potato disease estimation.","PeriodicalId":406162,"journal":{"name":"Cohesive Journal of Microbiology & Infectious Disease","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128653429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Bacteremia due to Opportunistic Infection in Times of Pandemic","authors":"J. Dreyse","doi":"10.31031/cjmi.2021.04.000600","DOIUrl":"https://doi.org/10.31031/cjmi.2021.04.000600","url":null,"abstract":"","PeriodicalId":406162,"journal":{"name":"Cohesive Journal of Microbiology & Infectious Disease","volume":"169 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114835599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Detecting Total Serum Testosterone by Chemiluminescence Immunoassays, Radioactive Assays, and Liquid-Chromatography Tandem Mass Spectrometry: A Comparative Study","authors":"Fenghua Chen","doi":"10.31031/cjmi.2021.04.000598","DOIUrl":"https://doi.org/10.31031/cjmi.2021.04.000598","url":null,"abstract":"Aim: LC-MS/MS is still considered the gold standard for detecting steroid hormones in clinic. The aim of this study was to compare the analytical sensitivity of chemiluminescence immunoassays (CLIA) platforms, radioimmunoassay (RIA) platform, and high-performance liquid-chromatography tandem mass spectrometry (LC-MS/MS) platform for detecting total testosterone (tTES) in human samples (males and females). Method: Sixty-nine healthy male serum samples and 114 female serum samples were collected. The serum testosterone was detected by automatic immunoassay systems of Beckman and SIEMENS, radioimmunoassay, and LC-MS/MS. Pearson’s correlation coefficients were calculated to assess the agreement of other platforms with LC-MS/MS. Result: When evaluating TES in males, the results detected by SIEMENS2000 had a good correlation with LC-MS/MS (r=0.986), followed by Beckman DXI800, while RIA showed a weak correlation. There was no statistical difference between the results detected by SIEMENS2000 compared to those detected by DXI800 (p=0.87). When evaluating TES in females, DXI800 showed a good correlation with LC-MS/ MS (r=0.8784). SIEMENS2000 had most of the detected values below its low limit, and the SIEMENS2000 results with definite values were not statistically different compared to DXI800 results. Conclusion: The results detection platforms (SIEMENS2000 and DXI800) both had a better correlation with those of LC-MS/MS than RIA. Yet, Siemens 2000 had better consistency with LC-MS/MS in detecting the serums with low tTES value than DXI800. the samples with definite values; were above the lowest limit of Simense (0.69nmom/L). The samples’ values the RIA obviously The correlation coefficient between the two immunoassay platforms and the LC-MS/MS was higher than that between RIA and LC-MS/MS in males. We further analyzed the samples’ results below 0.69nmol/L when using two chemiluminescence platforms.","PeriodicalId":406162,"journal":{"name":"Cohesive Journal of Microbiology & Infectious Disease","volume":"78 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132535180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparative Antimicrobial Studies between Commercial Deodorants, Alum, Sodium Bicarbonate and Lemon Against Sweat Odor Bacteria","authors":"Atta El","doi":"10.31031/cjmi.2021.04.000597","DOIUrl":"https://doi.org/10.31031/cjmi.2021.04.000597","url":null,"abstract":"","PeriodicalId":406162,"journal":{"name":"Cohesive Journal of Microbiology & Infectious Disease","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133379340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Pandemic Outbreak and Mortality Associated with Coronavirus Disease-19 in US and European Countries","authors":"Nazish Huma Khan","doi":"10.31031/cjmi.2021.03.000596","DOIUrl":"https://doi.org/10.31031/cjmi.2021.03.000596","url":null,"abstract":"","PeriodicalId":406162,"journal":{"name":"Cohesive Journal of Microbiology & Infectious Disease","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123821419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Risk of Forming Stealth Adapted Coronaviruses with the Using of Covid-19 Vaccines Analogy with Stealth Adapted Monkey Cytomegaloviruses Resulting from the Use of Polio Vaccines","authors":"W. J. Martin","doi":"10.5281/ZENODO.4489960","DOIUrl":"https://doi.org/10.5281/ZENODO.4489960","url":null,"abstract":"","PeriodicalId":406162,"journal":{"name":"Cohesive Journal of Microbiology & Infectious Disease","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128986058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"We were not Prepared for Covid-19-But we Should Have Been","authors":"Vikrant Minhas","doi":"10.31031/cjmi.2020.03.000595","DOIUrl":"https://doi.org/10.31031/cjmi.2020.03.000595","url":null,"abstract":"","PeriodicalId":406162,"journal":{"name":"Cohesive Journal of Microbiology & Infectious Disease","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132982706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Why Science Needs a New PR Rep","authors":"Vikrant Minhas","doi":"10.31031/cjmi.2020.03.000594","DOIUrl":"https://doi.org/10.31031/cjmi.2020.03.000594","url":null,"abstract":"","PeriodicalId":406162,"journal":{"name":"Cohesive Journal of Microbiology & Infectious Disease","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134228323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A Cautionary Tale: What if Stealth Adapted Viruses Incorporate the Covid-19 Spike Protein Coding mRNA into Their Genome?","authors":"W. J. Martin","doi":"10.31031/cjmi.2020.03.000593","DOIUrl":"https://doi.org/10.31031/cjmi.2020.03.000593","url":null,"abstract":"Stealth adaptation a generic evasion mechanism, with all viruses It the deletion or mutation of the genes coding for the relatively few virus components typically targeted by the cellular immune system. A further characteristic of certain stealth adapted viruses is the incorporation of additional genetic sequences of cellular and bacterial origins These “renegade” genetic sequences can be subsequently transmitted between humans as components of infectious stealth adapted viruses. The best characterized stealth adapted virus, referred in GenBank as stealth virus a derivative of an African green monkey simian cytomegalovirus (SCMV) It cultured a the chronic fatigue (CFS) A virus (stealth virus-2) psychosis Stealth virus-1 cloned and complete or partial DNA sequences of 248 clones submitted GenBank. Of these clones, 200 of the sequences correspond to regions of the SCMV genome. The sequences are unevenly distributed over the genome of the originating SCMV, with clear major deletions and genetic instability. Fourteen clones genetic sequences that were derived from non-coding regions of the human genome. These incorporated cellular sequences also undergone genetic changes indicative of the genetically stealth adapted virus genome Thirty-four sequences from stealth virus-1 protein-coding sequences. These sequences coinfection of with both stealth adapted and intracellular bacteria, with the bacteria-derived sequences specific metabolic functions and/or substituting for some of the ordinary capsids The overall genome of stealth virus-1 is genetically fragmented the likelihood of the cellular and bacteria-derived sequences incorporated into the virus genome by the cross-linking of fragments from the Cellular sequences incorporated into the stealth","PeriodicalId":406162,"journal":{"name":"Cohesive Journal of Microbiology & Infectious Disease","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121111516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"An Initial Profile and Virological Response of Sars-Cov-2 Infected Patients Admitted to Infectious Diseases Hospital of Northern India","authors":"S. Pandey","doi":"10.31031/cjmi.2020.03.000592","DOIUrl":"https://doi.org/10.31031/cjmi.2020.03.000592","url":null,"abstract":"","PeriodicalId":406162,"journal":{"name":"Cohesive Journal of Microbiology & Infectious Disease","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130422302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}