{"title":"Effects of Leukocyte Extract from the American Alligator (Alligator mississippiensis) on Antibiotic-Resistant Bacteria","authors":"V. Machha, P. Spencer, Mark Merchant","doi":"10.2174/1874336601104010009","DOIUrl":"https://doi.org/10.2174/1874336601104010009","url":null,"abstract":"Treatment of clinical isolates of human pathogenic bacteria, which were known to be resistant to multiple commonly-used antibiotics, with refined leukocyte extracts from the American alligator (Alligator mississippiensis) resulted in a time- and concentration-dependent inhibition of bacterial proliferation. The alligator leukocyte extract exhibited the strongest antibacterial effect on Pseudomonas aeruginosa followed by Enterococcus faecium and then Klebsiella pneumonia. The antibacterial activities were acid-soluble, heat-stable at 70 o C for one h, sensitive to protease treatment, and did not require divalent metal ions for antibacterial activity. Collectively, these data strongly suggest that the molecule(s) responsible for the observed antibacterial activities are small, cationic antimicrobial peptides.","PeriodicalId":404991,"journal":{"name":"The Open Zoology Journal","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116734200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of Different Oxygen Concentrations on Physiological Energetics of Blue Shrimp, Litopenaeus stylirostris (Stimpson)","authors":"R. Denisse","doi":"10.2174/1874336601104010001","DOIUrl":"https://doi.org/10.2174/1874336601104010001","url":null,"abstract":"The influence of different oxygen concentrations on the bioenergetics parameters, osmoregulatory capacity and O:N atomic ratio of the blue shrimp, Litopenaeus stylirostris (Stimpson), was determined under laboratory conditions; shrimp were acclimated at 28 oC and 25psu and exposed to three different oxygen concentrations 2, 4 and 6 mg L -1 . The critical oxygen level (COL) was determined at 5.5 mg L -1 . Growth, oxygen consumption (R), ammonium excretion (U), scope for growth (P) and apparent heat increment (AHI) were affected significantly (P < 0.05) when the organisms were exposed at different oxygen concentrations. The highest energy invested for routine metabolism and ammonium excretion was obtained in the animals maintained at 2 mg L -1 . The exposure of organisms to oxygen concentration (2 mg L -1 ) increased three times the feces production (23.59%). The high quantity of energy channeled to scope for growth (2756 J g - 1 day -1 d.w.) was obtained in the shrimp acclimated to an oxygen concentration of 6 mg L -1 . The O:N atomic ratio calculated for the juveniles indicated a catabolism of proteins for the organisms maintained in the lowest oxygen concentration. In the oxygen concentrations of 2 and 4 mg L -1 the blue shrimp juveniles modified their osmoregulatory capacity (OC) changing the osmoregulatory pattern the isosmotic to hypo-osmotic. We recommend maintaining Litopenaeus stylirostris at 6 mg L -1 and (25psu), these conditions for blue shrimp juveniles would enhance production in","PeriodicalId":404991,"journal":{"name":"The Open Zoology Journal","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132749849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A Review of Calcitonin Receptor Expression in Embryonic, Foetal and Adult Tissues, with an Hypothesis on the Connection Between Expression During Foetal Development and Disease~!2009-01-09~!2009-04-15~!2009-05-14~!","authors":"P. Wookey","doi":"10.2174/1874336600902010053","DOIUrl":"https://doi.org/10.2174/1874336600902010053","url":null,"abstract":"","PeriodicalId":404991,"journal":{"name":"The Open Zoology Journal","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115457468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yi-fan Chen, S. Jeng, Ming-Chyuan Chen, J. Gwo, Yung-Sen Huang
{"title":"Correlation Between PTEN and VEGF Expressions During the Development of Gas Gland in Japanese Eel (Anguilla japonica) Stimulated by Exogenous Hypophyseal Factors","authors":"Yi-fan Chen, S. Jeng, Ming-Chyuan Chen, J. Gwo, Yung-Sen Huang","doi":"10.2174/1874336600801010001","DOIUrl":"https://doi.org/10.2174/1874336600801010001","url":null,"abstract":"Organogenesis requires support of the vascular system and control of proliferation activity. VEGF, a selective mitogen for vascular endothelial cells, is important for vessel development. PTEN, known as a tumor suppressor, regu- lates cell size and modulates VEGF-mediated signaling on angiogenesis. However, it is not yet clear if PTEN controls tis- sue mass by suppressing angiogenesis provoked by VEGF and their correlations in postnatal tissue development in vivo. We used a primitive vertebrate, Japanese eel (Anguilla japonica), to study this question. Swim bladder gas glands (a so- matic tissue composed mainly by the capillaries) from normal eels or eels injected with hypophyseal factors were used to examine the expression of VEGF A, PTEN, IGF-1, and Flk-1 by RT-PCR. Two forms of PTEN (PTEN a, long form, and PTEN b, short form) cDNA have been cloned. There was no correlation between VEGF expression and gas gland tissue mass while a negative correlation between PTEN expression and tissue mass was noted; further, a higher correlation be- tween the tissue mass and the ratio of VEGF to PTEN was shown. In summary, we have shown the involvement of VEGF and PTEN in postnatal tissue development, the ratio of VEGF to PTEN may represent the growth status of the tissue.","PeriodicalId":404991,"journal":{"name":"The Open Zoology Journal","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122160070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Ferretti, S. Porrini, F. Zalli, L. Cellini, G. Paci, M. Bagliacca
{"title":"How Many Pheasants (Phasianus colchicus L.) can be Removed? A Study on Small- Protected Areas in Italy~!2008-12-22~!2009-02-05~!2009-04-21~!","authors":"M. Ferretti, S. Porrini, F. Zalli, L. Cellini, G. Paci, M. Bagliacca","doi":"10.2174/1874336600902010042","DOIUrl":"https://doi.org/10.2174/1874336600902010042","url":null,"abstract":"In Italy the maintenance and the increase of wild pheasant populations is mainly obtained in small-Protected Areas (PA) suitable for wildlife reproduction. In these areas a part of the resident population is regularly captured and transferred to the hunting zones in the winter time. The aim of the study is to determine the right number of pheasant to be removed from this protected area without causing damage and thus maintaining a balance in the resident population. Since 2000, flush counts census were conducted in 30 PAs in the Florence province during the summer time (post-reproduction period), using dogs experienced at rooting out the pheasants. We made estimates for each PA in the number of adult males, adult females and sexually undifferentiated young pheasants. We calculated post-reproduction density and the ratios of young/adults and males/females. These parameters were used to construct a model to predict the pheasants catches in the next winter. The study showed that the minimum census surface covered must be more than 9% of the total area studied. Smaller coverage gave biased estimations of pheasant numbers to be caught. The best generic model, to be used for surfaces between 297 and 1385 ha, located in Mediterranean habitats, was the following: total number of pheasants to be captured and relocated = -10.3 + 0.15*total number of female pheasants estimated in the protected area + 0.14* total number of young pheasants estimated in the protected area + 0.04*total surface of the protected area (R = 0.48).","PeriodicalId":404991,"journal":{"name":"The Open Zoology Journal","volume":"189 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117315389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Sciarrillo, A. Capaldo, S. Valiante, A. Sellitti, V. Laforgia, M. Falco
{"title":"Thyroid Hormones as Potential Early Biomarkers of Exposure to Nonylphenol in Adult Male Lizard (Podarcis sicula)~!2009-10-05~!2010-02-25~!2010-05-17~!","authors":"R. Sciarrillo, A. Capaldo, S. Valiante, A. Sellitti, V. Laforgia, M. Falco","doi":"10.2174/1874336601003020017","DOIUrl":"https://doi.org/10.2174/1874336601003020017","url":null,"abstract":"The thyroid has been shown to be a target organ of environmental chemicals, specifically endocrine disrupting contaminants. Reptiles are particularly suitable as contaminant biomonitors, due to their persistence in a variety of habitats, wide geographic distribution, longevity, and, in many cases, site fidelity. Nonylphenol, an estrogenic-like compound, can induce vitellogenin synthesis in males and immature reptilian species, but little is known about its effects on thyroid hormones balance. The present study evaluated the potential effects of an acute exposure to nonylphenol (i.p. injected) on the thyroid of the lizard Podarcis sicula. Nonylphenol induced a significant decrease of T4 and T3 plasma levels, in agreement with the decrease of the epithelial cell height; the nuclei of the thyroid cells were small and elongated, with dense chromatin and a greatly reduced cytoplasm. The colloid was retracted with few reabsorption vacuoles. Moreover, nonylphenol administration significantly inhibited plasma thyroid-stimulating hormone levels, thereby altering the thyroid function. This study highlights how the structural and functional disruption of the thyroid gland in non-target organisms as the lizard might also have an environmental aetiology. In conclusion, nonylphenol was suspected to inhibit the thyroid hormones balance, suggesting the thyroid should be included among the other endocrine glands, susceptible to endocrine","PeriodicalId":404991,"journal":{"name":"The Open Zoology Journal","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127960962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Mate Recognition, Species Boundaries and the Fallacy of “Species Recognition”~!2009-03-28~!2009-06-10~!2009-06-25~!","authors":"B. Sullivan","doi":"10.2174/1874336600902010086","DOIUrl":"https://doi.org/10.2174/1874336600902010086","url":null,"abstract":"","PeriodicalId":404991,"journal":{"name":"The Open Zoology Journal","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122754228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Favorito, M. C. Grimaldi, Marco Coppola, I. Ferrandino
{"title":"Effects of Acute Cadmium Exposure on the Pituitary Gland of Podarcis sicula~!2009-11-18~!2010-03-08~!2010-05-14~!","authors":"R. Favorito, M. C. Grimaldi, Marco Coppola, I. Ferrandino","doi":"10.2174/1874336601003020030","DOIUrl":"https://doi.org/10.2174/1874336601003020030","url":null,"abstract":"Reptiles are rarely used in studies on the possible toxic effects of heavy metals even if they are susceptible to the accumulation of persistent pollution due also to their presence in a variety of habitats. Cadmium is a heavy metal, a significant environmental pollutant and an endocrine disruptor. Therefore the aim of this study was to analyze the cytotoxic effects of cadmium on the pituitary gland of the lizard Podarcis sicula after an acute exposure to this metal. The analysis were carried out after 2, 7 and 16 days following the intraperitoneal injection of a single and massive dose of cadmium chloride. The pituitary glands were analyzed by histological and immuhistochemical stains. Besides cadmium accumulation in brain was measured by atomic absorption spectrometry. Cadmium concentration increased in lizard brain lightly after 2 days and widely after 16 days. The tissue of the pituitary gland appeared slightly atrophied in a few areas only at 7 and 16 days after treatment. Moreover an increase in intensity of immunostaining and occurrence of some adenohypophyseal cells was revealed respect to control lizards. This evidence suggests an inhibitory effect of cadmium on the normal hormonal secretion. Evidently an acute cadmium exposure in P. sicula involves the accumulation of this metal in the brain but also the alteration of the normal endocrine function of the pituitary gland.","PeriodicalId":404991,"journal":{"name":"The Open Zoology Journal","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127557612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Anatomy and Histology of the Spiral Valve Intestine in Juvenile Australian Lungfish, Neoceratodus forsteri~!2009-02-25~!2009-04-08~!2009-06-02~!","authors":"M. Hassanpour, J. Joss","doi":"10.2174/1874336600902000062","DOIUrl":"https://doi.org/10.2174/1874336600902000062","url":null,"abstract":"","PeriodicalId":404991,"journal":{"name":"The Open Zoology Journal","volume":"100 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117239361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Simoniello, F. Trinchella, R. Scudiero, S. Filosa, C. Motta
{"title":"Cadmium in Podarcis sicula Disrupts Prefollicular Oocyte Recruitment by Mimicking FSH Action~!2009-10-06~!2010-01-27~!2010-05-17~!","authors":"P. Simoniello, F. Trinchella, R. Scudiero, S. Filosa, C. Motta","doi":"10.2174/1874336601003020037","DOIUrl":"https://doi.org/10.2174/1874336601003020037","url":null,"abstract":"Cadmium is a highly polluting heavy metal known to have undesirable effects on health in both animals and humans, targeting the kidneys, the liver and the vascular system. A wide spectrum of deleterious effects has been reported also on the reproductive organs and the developing embryo. Cadmium in fact is a strong endocrine disruptor that interferes with functioning of endogenous receptors and hormones causing detrimental effects on offspring production and","PeriodicalId":404991,"journal":{"name":"The Open Zoology Journal","volume":"60 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126353712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}