Himawan Himawan, S. Rahardjo, S. Suntoro, P. Setyono
{"title":"Remediation of Lead-contaminated Farmland Soil: Efficacy of Low-cost Natural Amendments in [Im]mobilization of Lead","authors":"Himawan Himawan, S. Rahardjo, S. Suntoro, P. Setyono","doi":"10.17503/AGRIVITA.V43I1.2592","DOIUrl":"https://doi.org/10.17503/AGRIVITA.V43I1.2592","url":null,"abstract":"The control of Pb mobility is a success key in remediation of contaminated soil. This research aimed to explore the effective low-cost amendments in mobilizing or immobilizing lead in contaminated soil, assess environmental risk, and evaluate important amendment properties. Lead-contaminated soil was incubated with 13 materials included chars, bioslurry, limes, rice husk, gypsum, citric acid, natural organic acids and the efficiency of lead immobilization (E) was studied. Soil incubated with 10% amendments was sequentially extracted to determine Pb geochemical fractions and its risk. Selected amendment properties were evaluated through a correlation study with E. The results showed that 6 amendments immobilized lead in the order of chars > gypsum > bioslurry > rice husk. Chicken manure and chicken bone biochars, effectively decreasing lead solubility, were the best immobilising amendments, redistributed lead into the residual fraction and remediated the soil from moderate to low environmental risk. The order of mobilizing amendments was: citric acid > baby orange juice > limes > tamarind > lemon juice > tomato. The citric acid was the most effective mobilizing amendment which increased soluble lead and shifted the soil into high environmental risk status. The three most important amendment properties in influencing soil-Pb mobility were pH, P, and Ca content.","PeriodicalId":402006,"journal":{"name":"Agrivita : Journal of Agricultural Science","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116312696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Rahardjo, E. Achadian, Achmad Fitriadi Taufiqurrahman, M. Hidayat
{"title":"Silica Fertilizer (Si) Enhances Sugarcane Resistance to The Sugarcane Top Borer Scirpophaga excerptalis Walker","authors":"B. Rahardjo, E. Achadian, Achmad Fitriadi Taufiqurrahman, M. Hidayat","doi":"10.17503/AGRIVITA.V1I1.2654","DOIUrl":"https://doi.org/10.17503/AGRIVITA.V1I1.2654","url":null,"abstract":"Sugarcane is an essential industrial that produce sugar in the world. Scirpophaga excerptalis attacks can reduce the productivity of sugarcane up to 34%. To enhance the resistance to multiple stresses, Silicon is one of the elements for its role. This research aimed to determine the effect of the provision of silica fertilizer as part of the Integrated Pest Management strategy. This research was conducted in the Plant Protection Department, Indonesian Sugar Research Institute. The compost given as treatment in this research was straw, cane, and corn composts. Organic silica fertilizer (compost) provides the same effect as inorganic silica fertilizer in increasing the induction of sugarcane resistance to sugarcane top borer (S. excerptalis). Rice straw compost was a compost fertilizer that provides the best sugarcane resistance to sugarcane top borer compared to sugarcane plants that treated with sugarcane and corn leave to compost","PeriodicalId":402006,"journal":{"name":"Agrivita : Journal of Agricultural Science","volume":"49 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134193617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Hetharie, S. Raharjo, E. Jambormias, Reny Tomasoa, A. Y. Wattimena
{"title":"Selection Indicators of Yield Components and Yield for Improvement of Local Sweet Potato under Water Stress","authors":"H. Hetharie, S. Raharjo, E. Jambormias, Reny Tomasoa, A. Y. Wattimena","doi":"10.17503/AGRIVITA.V43I1.1993","DOIUrl":"https://doi.org/10.17503/AGRIVITA.V43I1.1993","url":null,"abstract":"Genetic diversity is required for crop improvement against environmental stress. Astudy wasconducted to determine characters as selection indicators of water-stress tolerance, and of yield components and yield. The research method involved a one-factor experiment with 21 sweet potato clones, using a Completely Randomized Block design with three replicates. Water stress in this study mean that the bottom of plants was submerged in water at 2 and 3 months of age. Determination of selection indicators was conducted by estimating the coefficients of genetic and phenotypic variations, heritabilities, genetic advance and correlation test result. The characters of number of leaves, individual leaf area, leaf area per plant, leaf area index, stem length, internode length, number of tubers (storage root), individual tuber weight and yieldas selection indicators of water stress tolerance. Selection indicators of yield components consisted of less number of leaves, small leaf size, short stemsand number of branches, whereas selection indicators of yield were small leaf size, number of branches, number of tubers and individual tuber weight. Selection became effective and efficient when the selection indicators were influenced greatly by genetic factors.","PeriodicalId":402006,"journal":{"name":"Agrivita : Journal of Agricultural Science","volume":"48 9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123175924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Seyyed Ali Morovvat, R. Sadrabadi, Koroush Shojaei Noferest, A. S. Darban, M. Salati
{"title":"Effects of Foliar Application Chitosan and Salicylic Acid on Physiological Characteristics and Yield under Deficit Irrigation Condition","authors":"Seyyed Ali Morovvat, R. Sadrabadi, Koroush Shojaei Noferest, A. S. Darban, M. Salati","doi":"10.17503/AGRIVITA.V43I1.2796","DOIUrl":"https://doi.org/10.17503/AGRIVITA.V43I1.2796","url":null,"abstract":"Drought stress is recognized as one of the most important factors reducing crop yields worldwide. Many studies are underway on the foliar application of different compounds to mitigate the effects of drought stress on plants. This experiment was designed to investigate the effects of foliar application of chitosan and salicylic acid on yield and its components under drought stress conditions in potatoes. The experimental design was split-plot with three replications based on a completely randomized design. The main plots and the sub-plots were 12 by 6 m and 6 by 3 m, respectively. The main plots represented three levels of irrigation (100%, 80%, and 60% of available soil water). Treatments in sub-plots included control treatment, 0.5 g/l salicylic acid, 2 g/l chitosan, and combined treatment with chitosan and salicylic acids. The results showed a direct relationship between reduced irrigation and reduced yield. As drought stress increased, yield, yield component parameters, and the physiological indices of the crop declined. Under stress conditions, the biological yield was increased by the application of chitosan and salicylic acid. The highest yield in non-stress conditions was 45,717 kg/ha; for foliar application of 0.5 g/l salicylic acid and 2 g/l chitosan, the highest yield was 45,683 kg/ha.","PeriodicalId":402006,"journal":{"name":"Agrivita : Journal of Agricultural Science","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117256249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Exploration and Antifungal Assay of Endophytic Fungi as Biocontrol of Onion Purple Blotch Disease Caused by Alternaria porri (Ell) Cif In Vitro","authors":"Wita Firdausi, Liliek Sulistyowati, L. Q. Aini","doi":"10.17503/AGRIVITA.V43I1.2838","DOIUrl":"https://doi.org/10.17503/AGRIVITA.V43I1.2838","url":null,"abstract":"Purple blotch disease caused by Alternaria porri is the main destructive foliar disease of genus Allium, causing significant losses in yield of the crops. Recently, purple blotch disease is controlled by synthetic fungicides. However, fungicides have negative effects on the environment. Endophytic fungi can be used as an alternative control because a close symbiosis with the internal tissue of the host can minimize competition in new and complex ecosystems. This study aimed to explore and identify endophytic fungi that have the highest inhibition ability against A. porri and investigate the antagonistic mechanism. The method used in this study is an exploration of endophytic fungi, isolation of A. porri, in vitro antagonism tests, observation of the antagonistic mechanism, extraction of crude protein, SDS-PAGE, and identification. The antagonistic fungi that had the highest inhibition ability were identified as Penicillium citrinum with an inhibitory of 60.04%. Crude protein extracted from P. citrinum which showed inhibitory activity against A. porri is saturation level of ammonium sulfate 80% with a molecular weight of 40 kDa. This study implies that P. citrinum can inhibit the growth of A. porri through its anti fungi compounds. Further in vivo assays or field trials will need to be conducted in future studies.","PeriodicalId":402006,"journal":{"name":"Agrivita : Journal of Agricultural Science","volume":"101 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114261970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Control of Banana Wilt Disease Caused by Fusarium oxysporum Schlecht f.sp. cubense (E. F. Smith) Using Crab Shell Powder and Chitosan","authors":"W. Widodo, H. Harti, S. Wiyono","doi":"10.17503/AGRIVITA.V1I1.2780","DOIUrl":"https://doi.org/10.17503/AGRIVITA.V1I1.2780","url":null,"abstract":"This research was conducted to evaluate the effectiveness of crab shell powder and chitosan to control Fusarium wilt disease on bananas and to analyze the involved control mechanisms. The effectiveness of crab shell powder and chitosan to F. oxysporum f.sp. cubense was examined in a laboratory (in vitro) and greenhouse (in planta). In vitro evaluation showed that chitosan has an antifungal effect while crab shell powder did not. Application of crab shell powder and chitosan suppressed the disease in green house test. The concentrations of crab shell powder and chitosan that most effective to control Fusarium wilt incidences were 0.25% and 0.10% with an efficacy rate of 66.7% and 83.3%, respectively. The highest disease severity reduction was showed by crab shell powder 0.25% and chitosan 0.50% with an efficacy rate of 56.8% and 59.4%, respectively. Suppression of the disease might be due to the fungicidal effect of chitosan and the increase of the total population of bacteria and chitinolytic bacteria in the rhizosphere when banana seedling roots were treated with crab shell powder or chitosan. Experiment results using the split roots technique exhibited the role of crab shell powder and chitosan potentially to induce the resistance of banana to Fusarium wilt.","PeriodicalId":402006,"journal":{"name":"Agrivita : Journal of Agricultural Science","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122964916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Bioinspired Optimization of Germination Nutrients Based on Lactuca sativa Seedling Root Traits as Influenced by Seed Stratification, Fortification and Light Spectrums","authors":"Ronnie S. Concepcion, E. Dadios","doi":"10.17503/AGRIVITA.V43I1.2843","DOIUrl":"https://doi.org/10.17503/AGRIVITA.V43I1.2843","url":null,"abstract":"Ecophysiological stimulators directly affect root morphology, especially in the embryonic stage. To enhance crop germination, an understanding of the root traits under abiotic inducers is needed. In this study, the combined impacts of white and red-blue light spectrums, cold stratification, and seed fortification involving various concentrations of bioactive chemicals namely simple nutrient addition program solution, gibberellic acid, α-naphthaleneacetic acid with thiamine hydrochloride were evaluated on loose-leaf lettuce (Lactuca sativa var. Altima) seedling root architecture. The growth-promoting effects of these nutrients varied the growth rate and morphology of roots which are immediately shown during the radicle development. Integrated computer vision and computational intelligence were employed for phytomorphological signatures extraction of seedlings that were cultivated in a customized modulable spectrum experimental chamber (MSPEC). Root phenotype model was developed using graph-cut segmentation and region properties, and the ideal germination nutrient concentration was optimized using bioinspired models with firefly algorithm optimal result of 204.1 mg/L for nitrate, 238.15 mg/L for phosphate, and 158.08 mg/L for potassium. It was verified that lettuce seedlings can endure highly concentrated nutrients, however, it is more sensitive to phosphate as this macronutrient significantly promotes root growth with the increased whorl number on white light spectrum exposure with cold stratification.","PeriodicalId":402006,"journal":{"name":"Agrivita : Journal of Agricultural Science","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129283614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marheni Marheni, E. Martono, Octanina Sari Sijabat
{"title":"Exploration of Symbiotic Bacteria of Oryctes rhinoceros (Coleoptera: Scarabaeidae) Larvae from Oil Palm Empty Fruit Bunches","authors":"Marheni Marheni, E. Martono, Octanina Sari Sijabat","doi":"10.17503/AGRIVITA.V43I1.2301","DOIUrl":"https://doi.org/10.17503/AGRIVITA.V43I1.2301","url":null,"abstract":"Oil palm empty fruit bunches (OPEFB) were utilized as the source of organic fertilizer, but become a medium of Oryctes rhinoceros proliferation in oil palm plantations. O. rhinoceros uses cellulose, hemicellulose and lignin in the OPEFB as energy sources for larvae growth and development. In the process of breaking and combining complex compounds into simple molecules, they are establishing symbiotic relationship with bacteria residing in the digestive tract of O. rhinoceros larvae. Isolation of the symbiotic bacteria of the O. rhinoceros larvae was carried out with the aim of identifying bacteria in the digestive tract of the third instar found in the OPEFB. This research was done by using a descriptive method. Exploration of symbiotic bacteria of O. rhinoceros larvae was conducted through two stages. The first step was attained by isolating, growing and multiplying the bacteria and conducting biochemical testing. The second phase was accomplished by molecular testing for bacterial identification. The results showed that bacterial isolates were found and and after molecularly sequenced based on 6S rDNA, the species were identified as Bacillus stratosphericus, B. siamensis, B. cereus, Haemophilus parainfluenzae, Achromobacter xylosoxidans, and Alcaligenes faecalis. The research also found several species of bacteria originating from the hindgut larvae O. rhinoceros.","PeriodicalId":402006,"journal":{"name":"Agrivita : Journal of Agricultural Science","volume":"134 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123361827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Enik Nurlaili Afifah, R. H. Murti, T. R. Nuringtyas
{"title":"Comparison of Metabolomics Expression in The Root and Leaf of Resistance and Susceptible Tomato against Root-Knot Nematode","authors":"Enik Nurlaili Afifah, R. H. Murti, T. R. Nuringtyas","doi":"10.17503/agrivita.v42i3.2440","DOIUrl":"https://doi.org/10.17503/agrivita.v42i3.2440","url":null,"abstract":"Tomato plants that resist nematode produce the various biochemical compounds associated with defense mechanisms to root-knot nematode. The resistant plant allegedly expresses the biochemical compounds systemically not only in the infected area but also in all of the cells. This study aimed to compare the biochemical compounds in the root and leaf of the plant due to nematode attack. Resistant and susceptible tomato were used as plant material. Two treatments consisted of a control (not inoculated with nematode) and nematode inoculation were inoculated to all tomato plants. 50 mg of freeze-dried sample of root and leaf were taken for assessing metabolomics analysis. Tomato root resulted in 16 metabolites, while in the leaf detected 15 metabolites including amino acid, sugar compounds, and aromatic compounds. Root and leaf data showed the resistant plants had same metabolites that important for nematode resistant mechanism. The metabolites were α- and β-glucose, and caffeic acid than susceptible. Both root and leaf had the same concentrations of glucose and caffeic acid, that implied the mechanism of resistance tomato plants to root-knot nematodes expressed systemically throughout the plant.","PeriodicalId":402006,"journal":{"name":"Agrivita : Journal of Agricultural Science","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125996904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cytological Distinctions Between Timun Suri and Cucumber Discovered by Fluorescence In Situ Hybridization (FISH) Using 45S Ribosomal DNA Gene","authors":"A. Setiawan, A. Purwantoro, A. Wibowo","doi":"10.17503/agrivita.v42i3.2142","DOIUrl":"https://doi.org/10.17503/agrivita.v42i3.2142","url":null,"abstract":"The genus Cucumis including timun suri, melon and cucumber, is important horticultural crop of flowering plants. The dispute of timun suri terminology is leading to false positive result in nomenclature of timun suri in Indonesia. Although the molecular research on these species has been widely conducted, detailed information and precise evidences based on molecular cytogenetic approach are poorly investigated. Fluorescence in situ hybridization (FISH) technique was used to reveal the cytological distinctions among these species. Here, we conducted physical mapping of 45S ribosomal DNA (rDNA) on six accessions of Cucumis . We confirmed that timun suri cannot be classified as cucumber. The chromosome number of cucumber is 14 chromosomes, while timun suri and melon are 24 chromosomes. The number of 45S rDNA loci in cucumber, melon and timun suri was highly conserved. All cucumber accessions in this study had nine to ten 45S rDNA loci with strong and weak signal intensities located at proximal regions of the short arms. Whereas in melon and timun suri, the signals of two pairs of 45S rDNA were located at terminal and interstitial regions of the short arms. These were clear evidences to confirm that timun suri was cytogenetically closed to melon instead of cucumber.","PeriodicalId":402006,"journal":{"name":"Agrivita : Journal of Agricultural Science","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131081542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}