Agrivita : Journal of Agricultural Science最新文献_第10页

Efficient Strategies for Elimination of Phenolic Compounds During DNA Extraction from Roots of Pistacia vera L. 黄连木根DNA提取过程中酚类化合物的有效去除策略。

Agrivita : Journal of Agricultural Science Pub Date : 2017-08-07 DOI: 10.17503/agrivita.v39i3.734

S. Rajaei, Rana Sabagh Farshi, Maryam Moazzam Jazi, S. Seyedi

{"title":"Efficient Strategies for Elimination of Phenolic Compounds During DNA Extraction from Roots of Pistacia vera L.","authors":"S. Rajaei, Rana Sabagh Farshi, Maryam Moazzam Jazi, S. Seyedi","doi":"10.17503/agrivita.v39i3.734","DOIUrl":"https://doi.org/10.17503/agrivita.v39i3.734","url":null,"abstract":"Optimization of DNA extraction protocols for plant tissues and including endophytic microorganisms is a critical step of advanced plant-microbe interaction in agricultural studies. Pistachio ( Pistacia vera L.) root tissue contains high levels of polyphenols have been known as major extract contaminants and inhibitors of enzymatic activities during amplification. The present study aimed to develop reliable strategies to purify DNA from Pistachio root samples. Inhibiting substances were removed from DNA through a process including extraction with hot detergent contains SDS-Tris- EDTA, AlNH4(SO4)2.12H2O as chemical coagulating factor and CTAB-NaCl. Following typically organic extraction/alcohol precipitation, denaturing agarose electrophoresis performed to purify probable remain contaminants. The purified DNA was enough free of polyphenols based upon loss of color and spectral quality (260/230>1.6) and efficiently amplified during polymerase chain reaction particularly in the present of GC-clamp primers. This method proved well with detection of Glomus sp. (arbuscular mycorrhiza fungi) associated with Pistacia vera L. using denaturing gradient gel electrophoresis (DGGE).","PeriodicalId":402006,"journal":{"name":"Agrivita : Journal of Agricultural Science","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128506539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The Influence of Organic and Inorganic Fertilizers on the Growth and Yield of Green Bean, Phaseolus vulgaris L. Grown in Dry and Rainy Season 有机肥和无机肥对旱季和雨季绿豆生长和产量的影响

Agrivita : Journal of Agricultural Science Pub Date : 2017-08-04 DOI: 10.17503/agrivita.v39i3.646

M. Santosa, M. D. Maghfoer, Hagus Tarno

引用次数: 11

Susceptibility of Maize Genotypes to Maize Weevil Sitophilus Zeamais Motsch. (Coleoptera: Curculionidae) 玉米基因型对玉米象甲的敏感性研究。(鞘翅目:象甲科)

Agrivita : Journal of Agricultural Science Pub Date : 2017-08-04 DOI: 10.17503/agrivita.v39i3.1278

B. Rahardjo, L. P. Astuti, A. Sugiarto, A. Rizali

{"title":"Susceptibility of Maize Genotypes to Maize Weevil Sitophilus Zeamais Motsch. (Coleoptera: Curculionidae)","authors":"B. Rahardjo, L. P. Astuti, A. Sugiarto, A. Rizali","doi":"10.17503/agrivita.v39i3.1278","DOIUrl":"https://doi.org/10.17503/agrivita.v39i3.1278","url":null,"abstract":"One possibility to protect the maize from storage pests is by developing genotypes that resistant to maize weevil (Sitophilus zeamais Motsch). The research was aimed to evaluate the susceptibility of several maize genotypes including local variety to the maize weevil. Six genotypes i.e. SJA, G10-12-20, G10-1-3, G10-1-7, G10-1-20 and G10-1-17 and Tosari as local variety were used. No-choice assay under laboratory condition with observed variables was laid an egg and F1 progeny number, the median time of development and weight loss of infested kernel. The susceptibility index was assessed using Dobie’s susceptibility index. Results showed that the number of eggs laid by five females and infested samples weight loss were not significantly different between six maize genotypes as well as local variety. In contrast, the number of F1 progeny emerged was significantly different. Based on the susceptibility index, the maize genotype of G10-1-3 and G10-1-17 were resistant to the weevil. While other maize genotypes and local variety were considered as moderate resistant. Based on analysis of chemical and physical characteristic revealed that maize genotypes with higher of the total phenolic compound and physical hardness tend to resist from weevil damaged.","PeriodicalId":402006,"journal":{"name":"Agrivita : Journal of Agricultural Science","volume":"399 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133579928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Quantification of BRIS Soil Bacteria at Tembila, Besut Terengganu Besut Terengganu Tembila土壤细菌数量测定

Agrivita : Journal of Agricultural Science Pub Date : 2017-08-02 DOI: 10.17503/AGRIVITA.V39I3.1292

Z. Mustapha, N. Mat, R. Othman, A. Zakaria

{"title":"Quantification of BRIS Soil Bacteria at Tembila, Besut Terengganu","authors":"Z. Mustapha, N. Mat, R. Othman, A. Zakaria","doi":"10.17503/AGRIVITA.V39I3.1292","DOIUrl":"https://doi.org/10.17503/AGRIVITA.V39I3.1292","url":null,"abstract":"A study was made to determine total number of bacteria living in the rhizosphere of three common plants at BRIS soil namely Acacia mangium , Melaleuca cajuputi Powell and Eleusine indica that grow on field. Soil samples were collected from each plant’s rhizosphere at three different locations of UniSZA Apiary, AGROPOLIS Research Farm and FBIM Teaching Farm at UniSZA, Besut Campus on May and November 2015. Enumeration of total bacteria by standard plate counts found that Acacia’s rhizosphere at AGROPOLIS Research Farm showed the highest CFU g -1 in November 2015 for aerobic (6.14 x 10 6 ) and anaerobic (3.97 x 10 6 ) bacteria. Acacia’s rhizosphere from UniSZA Apiary showed the highest CFU g -1 value in November 2015 for nitrogen fixing bacteria (aerobic; 1.87 x 10 6 , anaerobic; 1.5 x 10 6 ) and phosphate solubilization bacteria (aerobic; 1.4 x 10 6 , anaerobic; 1.41 x 10 6 ). The rainy season on November gave a higher bacterial CFU g -1 number (20-44 %) compared to the dry season on May for all different plants and locations. The finding showed that although BRIS soil is the problematic and unfertile soil, it has a large number of bacterial colony that might be beneficial to be exploited for enhancing plant growth and soil fertility.","PeriodicalId":402006,"journal":{"name":"Agrivita : Journal of Agricultural Science","volume":"105 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128016651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Strategy of Soybean Management (Glycine max L.) to Cope with Extreme Climate Using CropSyst© Model 基于CropSyst©模型的大豆管理策略(Glycine max L.

Agrivita : Journal of Agricultural Science Pub Date : 2017-08-02 DOI: 10.17503/AGRIVITA.V39I3.1020

A. Aminah, A. Ala, Y. Musa, R. Padjung, K. Kaimuddin

{"title":"Strategy of Soybean Management (Glycine max L.) to Cope with Extreme Climate Using CropSyst© Model","authors":"A. Aminah, A. Ala, Y. Musa, R. Padjung, K. Kaimuddin","doi":"10.17503/AGRIVITA.V39I3.1020","DOIUrl":"https://doi.org/10.17503/AGRIVITA.V39I3.1020","url":null,"abstract":"This research was carried out to verify the CropSyst © plant model from experimental data in a soybean field and to predict planting time along with its potential yield. The researches were divided into two stages. First stage was a calibration for model on field from June to September 2015. Second stage was the application of the model. The required data models included climatic, soil and crop’s genetic data. There were relationship between the obtained data in field and the simulation from CropSyst © model which was indicated by 0.679 of Efficiency Index (EF) value. This meant that the CropSyst © model was well used. In case of Relative Root Mean Square Error (RRMSE), it was shown at 2.68 %. RRMSE value described that there was a 2.68 % error prediction between simulation and actual production. In conclusion, CropSyst © can be used to predict the suitable planting time for soybean and as the result, the suitable planting time for soybean on the dry land is the end of rainy season (2 June 2015). Tanggamus variety is the most resistant variety based on slow planting time, because the decreased percentage of production was lower (8.3 %) than Wilis (26.3 %) and Anjasmoro (43.0 %).","PeriodicalId":402006,"journal":{"name":"Agrivita : Journal of Agricultural Science","volume":"49 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132527074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Variability in Morpho-physiology, Tuber Yield and Starch Content of Several Arrowroot Populations in Garut District Garut地区几个竹芋居群形态生理、块茎产量和淀粉含量的变异

Agrivita : Journal of Agricultural Science Pub Date : 2017-08-01 DOI: 10.17503/agrivita.v39i3.1002

A. Rohandi, Budiadi Budiadi, S. Hardiwinoto, E. Harmayani, D. Sudrajat

{"title":"Variability in Morpho-physiology, Tuber Yield and Starch Content of Several Arrowroot Populations in Garut District","authors":"A. Rohandi, Budiadi Budiadi, S. Hardiwinoto, E. Harmayani, D. Sudrajat","doi":"10.17503/agrivita.v39i3.1002","DOIUrl":"https://doi.org/10.17503/agrivita.v39i3.1002","url":null,"abstract":"Arrowroot ( Maranta arundinacea L.) is an important starchy plant which has potential utilization for food,industry and medicine. The study was undertaken to assess the variability in morpho-physiological characters, tuber yield and starch content of 23 arrowroot populations in Garut District, West Java. The result showed some significant differences in morpho-physiological characteristics, tuber yield and starch content. Growth characteristics revealed that Cilawu population recorded the highest values for some characters: high, leaf number, above growth biomass and tuber biomass. Cikajang population showed the highest tuber yield (210.6 g per plant) followed by Cilawu population (134 g per plant). Cikajang and Cilawu populations also provided the highest tuber biomass, 46.6 g and 60.0 g respectively. Cilawu population was a second population producing the highest starch content (26.1 %) after Cibatu population (27 %). The higher heritability coupled with genetic advance revealed for fresh tuber yield indicated that selection on basis of the character may be helpful to improve arrowroot yield. Most of the growth characteristics had not significant correlation indicating that the characteristics are not good indicator for selection. Cilawu, Cikajang and Cibatu populations have good potential to produce the high quality and quantity of tuber for arrowroot cultivation in Garut District.","PeriodicalId":402006,"journal":{"name":"Agrivita : Journal of Agricultural Science","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124003162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Development of Simple-Sequence Repeats Markers from Durian (Durio zibethinus Murr. cultv. Matahari) Genomic Library 榴莲简单重复序列标记的开发。cultv。Matahari)基因组图书馆

Agrivita : Journal of Agricultural Science Pub Date : 2017-08-01 DOI: 10.17503/AGRIVITA.V39I3.1171

P. J. Santoso, A. Pancoro, S. Suhandono, I. Aryantha

{"title":"Development of Simple-Sequence Repeats Markers from Durian (Durio zibethinus Murr. cultv. Matahari) Genomic Library","authors":"P. J. Santoso, A. Pancoro, S. Suhandono, I. Aryantha","doi":"10.17503/AGRIVITA.V39I3.1171","DOIUrl":"https://doi.org/10.17503/AGRIVITA.V39I3.1171","url":null,"abstract":"Simple sequence repeats have been proved as powerful markers and widely used in molecular breeding to reduce cycles and cost efective. The availability of the marker is, however, very limited in durian. This research aimed to develop SSR markers from durian genomic library. Genomic DNA was isolated from durian shoot leaf, whilst SSR motifs were isolated using membrane-based oligonucleotide enrichment hybridization protocol. Annotation made on the library found 527 unique motifs from 354 durian libraries which form 425 loci. The SSR motifs obtained were generally short repeats which reached 89.6 %, whilst longer repeats were found consisted of compound motifs. Eleven loci were selected as representative for further test to prove their informativity. A number of unique allels were successfully amplified from 17 durian genomes. The analysis showed the polymorphic information content (PIC) values ranged from 0.000 to 0.662 with an average of 0.390. The SSR loci also showed their ability to be used for durian diversity analysis as the evident that the loci could be used as genetic markers for assisting further durian breeding program.","PeriodicalId":402006,"journal":{"name":"Agrivita : Journal of Agricultural Science","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127790638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Responses of Cowpea Genotypes to Arbuscular Mycorrhiza 豇豆基因型对丛枝菌根的响应

Agrivita : Journal of Agricultural Science Pub Date : 2017-08-01 DOI: 10.17503/AGRIVITA.V39I3.937

A. Rohyadi, Rina Noviani, M. Isnaini

{"title":"Responses of Cowpea Genotypes to Arbuscular Mycorrhiza","authors":"A. Rohyadi, Rina Noviani, M. Isnaini","doi":"10.17503/AGRIVITA.V39I3.937","DOIUrl":"https://doi.org/10.17503/AGRIVITA.V39I3.937","url":null,"abstract":"A pot experiment was conducted under glasshouse conditions to evaluate response of some cowpea genotypes to Arbuscular Mycorrhiza. It was a 2 x 6 factorial experiment comprising factors of mycorrhizal inoculation (M) with AM fungal inoculum (M1) and without AM fungal inoculum (M0), and of plant genotypes (G) with Gf (a cowpea inbred line), Gm (a mung bean inbred line), Gh1, Gh2, Gh3 and Gh4 (the 1st, 2sd, 3rd and 4th generation of cowpea hybrids, derived from cross-breeding Gf x Gm as female and male parents, respectively), and arranged in a completely randomized design with 9 replicated pots, where plants were grown up for 14, 28 and 42 days before harvested serially. Responses of cowpea genotypes to colonization and contribution of mycorrrhizal symbiosis varied greatly. The intensity of plant roots colonized by mycorrhizal fungi was highest on Gf, and it was descent on the cowpea hybrids following their generation order. On the other hand, the highest in plant growth response to mycorrhizal function was on Gm, followed by Gh1 and Gh2. These results indicated that the cross-breed of cowpea and mung bean lines has generated cowpea hybrids that tend to benefit less from mycorrhizal symbiosis for their growth.","PeriodicalId":402006,"journal":{"name":"Agrivita : Journal of Agricultural Science","volume":"49 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125603965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Application of A New Bio-Formulation of Chaetomium cupreum For Biocontrol of Colletotrichum gloeosporioides Causing Coffee Anthracnose on Arabica Variety in Laos 新生物制剂铜毛菌在老挝阿拉比卡咖啡炭疽病防治中的应用

Agrivita : Journal of Agricultural Science Pub Date : 2017-07-27 DOI: 10.17503/AGRIVITA.V39I3.1070

S. Vilavong, K. Soytong

{"title":"Application of A New Bio-Formulation of Chaetomium cupreum For Biocontrol of Colletotrichum gloeosporioides Causing Coffee Anthracnose on Arabica Variety in Laos","authors":"S. Vilavong, K. Soytong","doi":"10.17503/AGRIVITA.V39I3.1070","DOIUrl":"https://doi.org/10.17503/AGRIVITA.V39I3.1070","url":null,"abstract":"The anthracnose pathogen was isolated from coffee leave and bean symptoms. Morphological and molecular phylogenetic data confirmed the species as Colletotrichum gloeosporioides . The pathogenicity of the isolate was also confirmed by detached leaf method which inoculated the virulent isolate into coffee leaves. The crude extracts with hexane, ethyl acetate and methanol solvents from Chaetomium cupreum CC3003 resulted significantly inhibited C. gloeosporioides that the ED 50 values of 13, 11 and 28 ppm, respectively. The bioactive substances of C. cupreum CC3003 expressed antifungal activity against C. gloeosporioides as can be seen in the abnormal appearance of spores. A powder bio-formulation of C. cupreum significantly resulted to reduce anthracnose disease of 54.77 %. The application of nano-rotiorinol, nano-trichotoxin and a spore suspension of C. cupreum reduced anthracnose incidence of 46.23, 42.71 and 18.59 %, respectively while the inoculated control had high anthracnose disease. The application of bio-formulation of C. cupreum in powder form, nano-rotiorinol, and nano-trichotoxin to reduce coffee anthracnose was reported for the first time in Lao PDR.","PeriodicalId":402006,"journal":{"name":"Agrivita : Journal of Agricultural Science","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129712036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Genetic Diversity and Population Structure of IRRDB 1981 and Wickham Rubber Germplasm Based on EST-SSR 基于EST-SSR的IRRDB 1981和Wickham橡胶种质遗传多样性及群体结构分析

Agrivita : Journal of Agricultural Science Pub Date : 2017-07-27 DOI: 10.17503/agrivita.v39i3.881

F. Oktavia, Kuswanhadi Kuswanhadi, Dini Dinarty, W. Widodo, S. Sudarsono

{"title":"Genetic Diversity and Population Structure of IRRDB 1981 and Wickham Rubber Germplasm Based on EST-SSR","authors":"F. Oktavia, Kuswanhadi Kuswanhadi, Dini Dinarty, W. Widodo, S. Sudarsono","doi":"10.17503/agrivita.v39i3.881","DOIUrl":"https://doi.org/10.17503/agrivita.v39i3.881","url":null,"abstract":"The accession of the IRDB 1981 (PN’81) population is a newly introduced and an important rubber tree germplasm while the Wickham clone is a commercial variety one. The objectives of this study were to assess the genetic diversity and the population structure of PN’81 populations and the Wickham clones using 15 EST-SSR loci. Results of the analysis showed that the evaluated SSR primers yielded polymorphic markers. The gSSR 268 primer pairs yielded the most informative markers while HBE 280 primers generated the lowest ones. Results of the genetic diversity analysis supported that the PN’81 population belonged to a single large natural population of rubber trees while the Wickham clones belonged to a different group than that of PN’81. The population structure analysis of the rubber accessions was also in agreement with the results of the genetic diversity analysis. The experiment also indicated that PN’81 populations would be useful for future rubber breeding in Indonesia, especially as the sources of parent clones for rubber tree hybridization programs and rubber tree genetic resource conservation.","PeriodicalId":402006,"journal":{"name":"Agrivita : Journal of Agricultural Science","volume":"146 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132244006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4