Comparison of Metabolomics Expression in The Root and Leaf of Resistance and Susceptible Tomato against Root-Knot Nematode

Abstract

Tomato plants that resist nematode produce the various biochemical compounds associated with defense mechanisms to root-knot nematode. The resistant plant allegedly expresses the biochemical compounds systemically not only in the infected area but also in all of the cells. This study aimed to compare the biochemical compounds in the root and leaf of the plant due to nematode attack. Resistant and susceptible tomato were used as plant material. Two treatments consisted of a control (not inoculated with nematode) and nematode inoculation were inoculated to all tomato plants. 50 mg of freeze-dried sample of root and leaf were taken for assessing metabolomics analysis. Tomato root resulted in 16 metabolites, while in the leaf detected 15 metabolites including amino acid, sugar compounds, and aromatic compounds. Root and leaf data showed the resistant plants had same metabolites that important for nematode resistant mechanism. The metabolites were α- and β-glucose, and caffeic acid than susceptible. Both root and leaf had the same concentrations of glucose and caffeic acid, that implied the mechanism of resistance tomato plants to root-knot nematodes expressed systemically throughout the plant.