Current Protocols in Neuroscience最新文献_第6页

Neocortical Microdissection at Columnar and Laminar Resolution for Molecular Interrogation 柱状和层流分辨率的新皮层显微解剖用于分子询问

Current Protocols in Neuroscience Pub Date : 2018-10-04 DOI: 10.1002/cpns.55

Koen Kole, Tansu Celikel

引用次数: 15

Mouse Models of Pain in Sickle Cell Disease 镰状细胞病小鼠疼痛模型

Current Protocols in Neuroscience Pub Date : 2018-09-28 DOI: 10.1002/cpns.54

Varun Sagi, Waogwende L. Song-Naba, Barbara A. Benson, Sonal S. Joshi, Kalpna Gupta

引用次数: 14

Measuring Pain Avoidance-Like Behavior in Drug-Dependent Rats 药物依赖大鼠疼痛回避行为的测量

Current Protocols in Neuroscience Pub Date : 2018-09-24 DOI: 10.1002/cpns.53

Amanda R. Pahng, Scott Edwards

引用次数: 7

Dual-Channel Photostimulation for Independent Excitation of Two Populations 两种群独立激发的双通道光刺激

Current Protocols in Neuroscience Pub Date : 2018-09-11 DOI: 10.1002/cpns.52

Bryan M. Hooks

引用次数: 2

Assessing Individual Neuronal Activity Across the Intact Brain: Using Hybridization Chain Reaction (HCR) to Detect Arc mRNA Localized to the Nucleus in Volumes of Cleared Brain Tissue 评估完整大脑中的单个神经元活动:使用杂交链式反应(HCR)检测清除脑组织中定位于细胞核的Arc mRNA

Current Protocols in Neuroscience Pub Date : 2018-06-26 DOI: 10.1002/cpns.49

Emily E. Kramer, Patrick E. Steadman, Jonathan R. Epp, Paul W. Frankland, Sheena A. Josselyn

{"title":"Assessing Individual Neuronal Activity Across the Intact Brain: Using Hybridization Chain Reaction (HCR) to Detect Arc mRNA Localized to the Nucleus in Volumes of Cleared Brain Tissue","authors":"Emily E. Kramer, Patrick E. Steadman, Jonathan R. Epp, Paul W. Frankland, Sheena A. Josselyn","doi":"10.1002/cpns.49","DOIUrl":"10.1002/cpns.49","url":null,"abstract":"Arc (activity-regulated cytoskeleton-associated protein) is an immediate early gene that may be used to label recently active neurons. Arc is transcribed following neuronal activity, and its mRNA is then rapidly transported to dendrites. This feature allows nuclear-localized Arc mRNA to define ensembles of recently active neurons in systems or circuit neuroscience. However, typical in situ hybridization techniques severely constrain the thickness of the tissue specimen (typically 20-µm brain slices). Here, we describe a protocol for visualizing intranuclear Arc mRNA in large (4 × 4 × 3 mm) volumes of intact mouse brain tissue. We combined a tissue clearing protocol (iDISCO+) with an advanced in situ hybridization technique (hybridization chain reaction [HCR]) to detect nuclear-localized Arc mRNA in whole, intact brain regions without the need for brain sectioning or reconstruction. We successfully applied this protocol to image ensembles of neurons of the basolateral amygdala in mice that are active following the recall of a conditioned fear memory. © 2018 by John Wiley & Sons, Inc.","PeriodicalId":40016,"journal":{"name":"Current Protocols in Neuroscience","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpns.49","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36258885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

A Compact Head-Mounted Endoscope for In Vivo Calcium Imaging in Freely Behaving Mice 用于自由行为小鼠体内钙成像的紧凑型头戴式内窥镜

Current Protocols in Neuroscience Pub Date : 2018-06-26 DOI: 10.1002/cpns.51

Alexander D. Jacob, Adam I. Ramsaran, Andrew J. Mocle, Lina M. Tran, Chen Yan, Paul W. Frankland, Sheena A. Josselyn

{"title":"A Compact Head-Mounted Endoscope for In Vivo Calcium Imaging in Freely Behaving Mice","authors":"Alexander D. Jacob, Adam I. Ramsaran, Andrew J. Mocle, Lina M. Tran, Chen Yan, Paul W. Frankland, Sheena A. Josselyn","doi":"10.1002/cpns.51","DOIUrl":"10.1002/cpns.51","url":null,"abstract":"Miniaturized fluorescence microscopes for imaging calcium transients are a promising tool for investigating the relationship between behavior and population-level neuronal activity in rodents. However, commercially available miniature microscopes may be costly and, because they are closed source, may not be easily modified based on particular experimental requirements. Here, we describe how to build and use a low-cost compact head-mounted endoscope (CHEndoscope) system for in vivo calcium imaging. The CHEndoscope uses an implanted gradient index lens along with the genetically encoded calcium indicator GCaMP6 to image calcium transients from hundreds of neurons simultaneously in awake behaving mice. This system is affordable, open source, and flexible, permitting modification depending on the particular experiment. This article describes in detail the assembly, surgical implantation, data collection, and processing of calcium signals using the CHEndoscope system. The aim of this open framework is to provide an accessible set of miniaturized calcium imaging tools for the neuroscience research community. © 2018 by John Wiley & Sons, Inc.","PeriodicalId":40016,"journal":{"name":"Current Protocols in Neuroscience","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpns.51","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36258945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 46

Behavioral Modulation by Social Experiences in Rodent Models 啮齿动物模型中社会经验对行为的调节

Current Protocols in Neuroscience Pub Date : 2018-05-16 DOI: 10.1002/cpns.50

Alexei Morozov

引用次数: 7

Fear Conditioning by Proxy: Social Transmission of Fear Between Interacting Conspecifics 代理的恐惧条件反射:相互作用的同种个体之间恐惧的社会传递

Current Protocols in Neuroscience Pub Date : 2018-04-26 DOI: 10.1002/cpns.43

Carolyn E. Jones, Laura Agee, Marie-H. Monfils

{"title":"Fear Conditioning by Proxy: Social Transmission of Fear Between Interacting Conspecifics","authors":"Carolyn E. Jones, Laura Agee, Marie-H. Monfils","doi":"10.1002/cpns.43","DOIUrl":"10.1002/cpns.43","url":null,"abstract":"We describe a method of social fear transmission to a discrete auditory cue in freely behaving rats. Extending beyond traditional observer/demonstrator paradigms, rats are allowed to physically interact and integrate cues from all sensory modalities. In the protocol described in this article, “observer” rats experience social fear conditioning through a proxy cage mate that serves as a “demonstrator” during retrieval of a cued fear memory. We find that a specific auditory cue can come to elicit fear expression in an animal with no foot shock experience simply by interacting with a conspecific expressing a conditioned response in the presence of an otherwise benign stimulus. In this “fear conditioning by proxy” paradigm, we have demonstrated that some, but not all, rats display conditioned responding (e.g., freezing) to a cue after interacting with a cage mate during fear memory retrieval. The amount of freezing exhibited by this fear conditioned “by proxy” rat 24 hr after learning critically depends on social influences, including social relationships and social interactions during learning. © 2018 by John Wiley & Sons, Inc.","PeriodicalId":40016,"journal":{"name":"Current Protocols in Neuroscience","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpns.43","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36339294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Functional Pituitary Tissue Generation from Human Embryonic Stem Cells 从人胚胎干细胞生成功能性垂体组织

Current Protocols in Neuroscience Pub Date : 2018-04-26 DOI: 10.1002/cpns.48

Mayuko Kano, Hidetaka Suga, Takatoshi Kasai, Chikafumi Ozone, Hiroshi Arima

{"title":"Functional Pituitary Tissue Generation from Human Embryonic Stem Cells","authors":"Mayuko Kano, Hidetaka Suga, Takatoshi Kasai, Chikafumi Ozone, Hiroshi Arima","doi":"10.1002/cpns.48","DOIUrl":"10.1002/cpns.48","url":null,"abstract":"The anterior pituitary gland produces several hormones essential for regulation of the somatic endocrine system. Deficiency of these hormones can cause life‐threatening diseases, including adrenal crisis. Pituitary tissue generated from human pluripotent stem cells is expected to provide better treatment than current hormone replacement therapy. During early mammalian development, the pituitary anlage (Rathke's pouch) develops from non‐neural ectoderm adjacent to the developing ventral hypothalamus. The close interaction between these two tissues is crucial for Rathke's pouch development and involves several signaling molecules. Early exposure of human embryonic stem cells in 3D floating culture to sonic hedgehog and bone morphogenetic protein 4 promoted the cells’ differentiation into oral ectoderm and, subsequently, hormone‐producing cells such as corticotrophs (adrenocorticotropic hormone–producing cells). The differentiation approach described herein, which induces the formation of pituitary tissue in contact with hypothalamic neural tissue, mimics mammalian pituitary development. The differentiated corticotrophs are functional, responding normally to both release and feedback signals. © 2018 by John Wiley & Sons, Inc.","PeriodicalId":40016,"journal":{"name":"Current Protocols in Neuroscience","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpns.48","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36339623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

A Brief Guide to Studying Fear in Developing Rodents: Important Considerations and Common Pitfalls 研究发育中的啮齿动物的恐惧的简要指南:重要的考虑和常见的陷阱

Current Protocols in Neuroscience Pub Date : 2018-04-26 DOI: 10.1002/cpns.44

Caitlin S.M. Cowan, Rick Richardson

引用次数: 10