Disease and Molecular Medicine最新文献

In vitro activities of colistin combined with imipenem, tigecycline or cefoperazone-sulbactam against multidrug-resistant Acinetobacter baumannii blood-stream isolates 粘菌素联合亚胺培南、替加环素或头孢哌酮舒巴坦对多药耐药鲍曼不动杆菌血流分离株的体外活性研究

Disease and Molecular Medicine Pub Date : 2016-12-31 DOI: 10.5455/DMM.20160830024755

O. Ak, Demet Hacıseyitoğlu, Y. Çağ, S. Gencer, F. Biteker, S. Özer

{"title":"In vitro activities of colistin combined with imipenem, tigecycline or cefoperazone-sulbactam against multidrug-resistant Acinetobacter baumannii blood-stream isolates","authors":"O. Ak, Demet Hacıseyitoğlu, Y. Çağ, S. Gencer, F. Biteker, S. Özer","doi":"10.5455/DMM.20160830024755","DOIUrl":"https://doi.org/10.5455/DMM.20160830024755","url":null,"abstract":"Acinetobacter baumannii has emerged as one of the most important nosocomial pathogens and multi-drug resistant (MDR) isolates are of great concern worldwide. The aim of the present study was to investigate the in vitro synergistic activity of colistin in combination with other antibiotics against MDR A. baumannii blood stream isolates. A total of 54 non-duplicate, MDR A. baumannii isolates from blood culture specimens obtained between June 2011 and July 2012 were included in the study. In vitro synergistic activity of colistin in combination with imipenem, tigecycline or cefoperazone-sulbactam against study isolates was investigated by Etest superimposing method and the fractional inhibitory concentration (FIC) index was calculated for each antibiotic combination. The most frequent synergistic effect of colistin was found in combination with tigecycline in only 7 isolates (13.0%). All three antibiotics were found to have synergistic effect with colistin in four isolates (7.4%). Of isolates, 46 (85.2%) showed additive effect of colistin in combination with cefoperazone-sulbactam or tigecycline, 45 (83.3%) with imipenem. We found synergistic activity of colistin with other study antibiotics in only a small number of isolates. Although Etest method is a practical method to investigate the synergistic activity, in case of choosing empirical treatment, colistin in combination with another antibiotic may be preferred.","PeriodicalId":399572,"journal":{"name":"Disease and Molecular Medicine","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128713545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Investigation of Differences In CRP, PCT, WBC and MPV In Gram-Negative, Gram-Positive and Fungal Bloodstream Infections 革兰氏阴性、革兰氏阳性和真菌血流感染中CRP、PCT、WBC和MPV差异的研究

Disease and Molecular Medicine Pub Date : 2016-12-31 DOI: 10.5455/DMM.20161112051623

A. Inci

{"title":"Investigation of Differences In CRP, PCT, WBC and MPV In Gram-Negative, Gram-Positive and Fungal Bloodstream Infections","authors":"A. Inci","doi":"10.5455/DMM.20161112051623","DOIUrl":"https://doi.org/10.5455/DMM.20161112051623","url":null,"abstract":"Sepsis, which develops as a result of inflammatory response against infection, is a signicant cause of mortality, especially in patients in intensive care unit. Early diagnosis and effective treatment of bacterial infections is life-saving. The aim of this study was investigate the differences in C-reactive protein (CRP), procalcitonin (PCT), white blood cell count (WBC) and mean platelet volume (MPV) levels in bloodstream infections caused by Gram- negative (GN), Gram-positive (GP) or fungal agents. We retrospectively evaluatd the patients with bloodstream infections hospitalized between 01.01.2014 and 01.01.2016. Identification of microorganisms was performed in Microbiology Laboratory. Blood cultures were performed by using BACTEC 9120 (Becton Dickinson, USA) automatized blood culture system. CRP, PCT, WBC and MPV levels, which were investigated simultaneously with blood culture, of patients in whose blood culture growth occured were investigated. Mean PCT, CRP, WBC and MPV values were compared in patients with different infection agents. A total of 74 patients were investigated. There were 35 (47%) patients with Gram-negative, 26 (35%) patients with Candida and 13 (18%) patients with Gram-positive microorganisms. The most common GN microorganism agents were was Acinetobacter, Candida albicans was the most common fungal agent and coagulase-negative staphylococci (CoNS) was the most common GP agent. The highest PCT values were in patients with Klebsiella, Escherichia coli and Acinetobacter infections, respectively. CRP and PCT values were higher in GN bacteremias compared to GP and fungal infections. We think that CRP and PCT levels may be beneficial for differentiation of GN bacteraemia, GP bacteraemia and fungal infections aand may be considered as a factor which may guide empirical antimicrobial treatments.","PeriodicalId":399572,"journal":{"name":"Disease and Molecular Medicine","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130137756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Interlukin-10 gene polymorphisms (-819T/C and -1082A/G) and Type 2 diabetes mellitus in North Indian population 白介素-10基因多态性(-819T/C和-1082A/G)与印度北部人群2型糖尿病的关系

Disease and Molecular Medicine Pub Date : 2016-12-31 DOI: 10.5455/DMM.20161003012258

Sushma Verma, H. Chandra, M. Banerjee

{"title":"Interlukin-10 gene polymorphisms (-819T/C and -1082A/G) and Type 2 diabetes mellitus in North Indian population","authors":"Sushma Verma, H. Chandra, M. Banerjee","doi":"10.5455/DMM.20161003012258","DOIUrl":"https://doi.org/10.5455/DMM.20161003012258","url":null,"abstract":"Diabetes is a metabolic disorder characterized by chronic hyperglycemia and impaired cytokine levels leading to inflammation. Interlukin-10 (IL-10) is an anti-inflammatory cytokine which acts as macrophage deactivator affecting the synthesis of TNF-a, IL-1, IL-6, IL-8 and GM-CSF. Single-nucleotide polymorphisms (SNPs) viz. -592A/C, -819T/C and -1082A/G in IL-10 promoter are associated with IL-10 production. Low IL-10 levels in T2DM cases may be regulated by such gene variants. The present study was undertaken to evaluate the association of two genetic polymorphisms viz. IL-10 -819T/C and -1082A/G with T2DM in a North Indian population. Blood samples from 402 subjects (201 each of controls and T2DM cases) were collected after ethical approval and individual written consent. All subjects were genotyped by polymerase chain reaction-restriction length polymorphism (PCR-RFLP) using specific primers and restriction enzymes. Genotypic, allelic, carriage rate frequencies were calculated and haplotypic analysis performed by SPSS (version 21.0) and SHEsis (online version). All biochemical parameters except WHR and TG showed significant association with T2DM (P<0.005). In the study population ‘TC’ genotype of -819T/C and ‘GG’ genotype of -1082A/G showed significant association with T2DM (P< 0.0001, OR=2.195; P<0.038, OR=1.946 respectively) while allelic frequencies did not show association. Individuals with genotypic combinations viz. CT/AG (+/-) and CT/GG (+/-, +/+) showed 2-4 times higher risk of developing T2DM while haplotype analysis did not show any association. All genotypes of IL-10 polymorphism showed significant association with biochemical parameters viz. BPS, BPD, F, PP and TC (P<0.007). The IL-10 gene polymorphisms therefore play important roles in determining diabetes susceptibility.","PeriodicalId":399572,"journal":{"name":"Disease and Molecular Medicine","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115209288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

An Update on Ebola Virus Epidemiology and Experimental Modalities 埃博拉病毒流行病学和实验方式最新情况

Disease and Molecular Medicine Pub Date : 2016-12-31 DOI: 10.5455/DMM.20160711041210

F. Şahiner, N. Ardıç

{"title":"An Update on Ebola Virus Epidemiology and Experimental Modalities","authors":"F. Şahiner, N. Ardıç","doi":"10.5455/DMM.20160711041210","DOIUrl":"https://doi.org/10.5455/DMM.20160711041210","url":null,"abstract":"Ebola virus disease (EVD), formerly known as Ebola hemorrhagic fever, is a re-emerging zoonotic disease that has caused periodic outbreaks since the first reported outbreak in 1976. In 2014, in West Africa, the largest outbreak of Ebola virus in history emerged, affecting close to 30,000 people; more than 10,000 of these individuals died. This virus, which causes fatal hemorrhagic fever in humans and non-human primates, has the highest mortality rate (25-90%) among the viral hemorrhagic fever diseases. Ebola virus is transmitted through the blood and body fluids of infected animals and humans, as well as through contaminated materials. The virus is often spread in the hospital setting, and it is classified as a biosafety level 4 agent. Accordingly, diagnostic procedures and infected patient care should be performed at the highest safety levels. The potential therapies and vaccines against EVD have continued developing or been used in emergency situations during last outbreak. Some of these include the chimpanzee adenovirus type-3 and recombinant vesicular stomatitis virus-Ebola vaccines and new treatment modalities, such as passive immunization, brincidofovir, and ZMapp. Although these trials have shown different levels of success, there are no standardized treatments or vaccines that have been approved for humans. Such prevention is the only way to reduce human infection and the spread of outbreaks and is primarily based on personal protection measures and raising awareness of the risk factors.","PeriodicalId":399572,"journal":{"name":"Disease and Molecular Medicine","volume":"33 6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128748128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Activation of hepatic AMPK by 17β-estradiol suppresses both nuclear receptor Nr2c2/TR4 and its downstream lipogenic targets, reduces gluconeogenic genes and improves insulin signaling 17β-雌二醇激活肝脏AMPK可抑制核受体Nr2c2/TR4及其下游脂肪生成靶点，减少糖异生基因并改善胰岛素信号传导

Disease and Molecular Medicine Pub Date : 2016-12-31 DOI: 10.5455/DMM.20160929104837

S. Al-Qahtani, G. Bryzgalova, S. Efendić, P. Berggren, N. Portwood

{"title":"Activation of hepatic AMPK by 17β-estradiol suppresses both nuclear receptor Nr2c2/TR4 and its downstream lipogenic targets, reduces gluconeogenic genes and improves insulin signaling","authors":"S. Al-Qahtani, G. Bryzgalova, S. Efendić, P. Berggren, N. Portwood","doi":"10.5455/DMM.20160929104837","DOIUrl":"https://doi.org/10.5455/DMM.20160929104837","url":null,"abstract":"Estrogen replacement reduces the frequency of type 2 diabetes (T2D), an effect which involves the suppression of hepatic glucose production. The objective of this study was to identify the hepatic mechanisms involved in the beneficial effects of 17β-estradiol (E2) on insulin sensitivity in mice exposed to long term (10 months) high fat diet (HFD) feeding. E2 treatment in HFD mice led to significant improvements in glycemic control. In the livers from these animals, Western blotting studies showed that E2 treatment led to significant increases in the activation state of the AMP-activated protein kinase (AMPK), in association with reduction of the nuclear receptor Nr2c2/TR4 both mRNA and protein levels, and suppression of downstream lipogenic gene expression. These effects were in parallel with up-regulation of hormone sensitive lipase (lipe) expression by E2. Increased fasting glucose levels following HFD feeding were associated with increases in the expression levels of the gluconeogenic genes, g6pt1 and pyruvate carboxylase, whilst E2 treatment significantly reduced their expression levels. The insulin signaling pathway was studied in the liver after acute insulin intervention. The phosphorylation states of AKT2 and FOXO1 were both decreased in HFD mice, and E2 treatment reversed these changes. In conclusion, E2 treatment reduced body weight and improved glycemic control in association with activation of hepatic AMPK, reduced expression of its downstream target Nr2c2/TR4, and consequent decreases in lipogenic gene expression. Together with increased triglyceride mobilization, these changes paralleled improved hepatic insulin signaling and reduced gluconeogenic gene expression.","PeriodicalId":399572,"journal":{"name":"Disease and Molecular Medicine","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116053969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of Crohn’s Disease and Celiac Disease: Causal link or only coincidence? 克罗恩病和乳糜泻的关联:因果关系还是巧合?

Disease and Molecular Medicine Pub Date : 2016-12-01 DOI: 10.5455/DMM.20161011084217

T. Duzenli, M. Kaplan, A. Tanoğlu, D. Altun, H. Çermik

引用次数: 0

Effects of hyperbaric oxygen therapy on acetaminophen induced nephrotoxicity and hepatotoxicity: the role of heme oxygenase-1 - 高压氧治疗对对乙酰氨基酚所致肾毒性和肝毒性的影响:血红素加氧酶-1 -的作用

Disease and Molecular Medicine Pub Date : 2016-09-30 DOI: 10.5455/DMM.20160802110754

Iclal Karatop-Cesur, Ş. Yıldız, G. Uzun, Y. Oztas, S. Sabuncuoğlu, Yasin Ilgaz, A. Kutlu, Eyüp Doğan, G. Çebi, E. Oztaş

{"title":"Effects of hyperbaric oxygen therapy on acetaminophen induced nephrotoxicity and hepatotoxicity: the role of heme oxygenase-1 -","authors":"Iclal Karatop-Cesur, Ş. Yıldız, G. Uzun, Y. Oztas, S. Sabuncuoğlu, Yasin Ilgaz, A. Kutlu, Eyüp Doğan, G. Çebi, E. Oztaş","doi":"10.5455/DMM.20160802110754","DOIUrl":"https://doi.org/10.5455/DMM.20160802110754","url":null,"abstract":"The aim of this study was to investigate the effects of hyperbaric oxygen (HBO) therapy on acetaminophen (APAP) induced renal and liver injudr and the role of heme oxygenase-1 (HO-1) activation. Wistar-Albino rats were randomly assigned into four groups. Control group received no treatment. APAP (3gr/kg) was administered by gastric lavage in APAP group. Animals in the APAP+HBO and APAP+zinc protoporphyrin (ZnPP)+HBO groups received HBO therapy (90 min at 2.5 atm), starting 1 hour after APAP administration, for 2 consecutive days.HO-1 activity was inhibited by ZnPP. APAP+ZnPP+HBO group received intraperitoneal 50 µmolkg ZnPP injection 30 minutes after APAP treatment and HBO therapy for 2 days. Serum and tissue samples were taken at 48 hours after APAP treatment. Renal and liver functions were evaluated by serum levels of urea, creatinine and transaminases. Lipid peroxidation and tissue levels of antioxidant enzymes were measure by ELISA. Tissue injury was evaluated by light microscopy.HO-1 level was determined by immunohistochemistry. HO-1 mRNA level was investigated by polymerase chain reaction (PCR). Serum transaminase levels significantly increased after APAP treatment (p","PeriodicalId":399572,"journal":{"name":"Disease and Molecular Medicine","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128724335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Sporotrichosis Mimicking Cellulitis 模拟蜂窝织炎的孢子菌病

Disease and Molecular Medicine Pub Date : 2016-09-01 DOI: 10.5455/DMM.20160621014443

G. Karagöz, A. Kadanalı, Ş. Çomoğlu, N. Ozdemir, Arzu Irvem

引用次数: 0

A Case of Anicteric Leptospirosis Presenting with Rectal Bleeding and Hyperpyrexia 无黄疸钩端螺旋体病以直肠出血和高热表现1例

Disease and Molecular Medicine Pub Date : 2016-06-30 DOI: 10.5455/DMM.20160614100129

H. Ilhan, M. Hatipoglu, A. Ilhan, E. Solmazgul, N. Ersoz, E. Polat, V. Turhan

引用次数: 0

Risk Factors Influencing Development Of Surgical Site Infection In Patients Who Were Operated Due To Endometrial Cancer 影响子宫内膜癌手术患者手术部位感染发生的危险因素

Disease and Molecular Medicine Pub Date : 2016-06-30 DOI: 10.5455/DMM.20160626073052

A. Inci, M. Talmac, V. Ulker, O. Akbayir, C. Numanoğlu

{"title":"Risk Factors Influencing Development Of Surgical Site Infection In Patients Who Were Operated Due To Endometrial Cancer","authors":"A. Inci, M. Talmac, V. Ulker, O. Akbayir, C. Numanoğlu","doi":"10.5455/DMM.20160626073052","DOIUrl":"https://doi.org/10.5455/DMM.20160626073052","url":null,"abstract":"Endometrial cancer is the most commonly encountered malignancy of female genital system. Surgery is the main treatment approach in endometrial cancer. The frequency of surgical site infections (SSI) has recently increased. Prediction of risk factors which may cause SSI and taking due precautions may provide a decrease in frequency of these infections. The aim of this study was to determine the risk factors for SSI after surgery for endometrial cancer. The medical records of patients who were operated due to endometrial cancer in Kanuni Sultan Suleyman Education and Research Hospital between January 1, 2015, and July 31, 2015, were retrospectively reviewed. The patients were divided into two groups; those that developed SSI following the operation and those that did not develop SSI. SSI was diagnosed based on Center for Disease Control and Prevention criteria. Ages, comorbid diseases, body mass index, American Society of Anesthesiologists (ASA) scores, smoking, durations of operation, the presence of drainage, blood transfusion, pre-operative hemoglobin level, and pre-operative glucose level of patients were recorded. A P < 0.05 was considered to be statistically significant. Of the 103 patients included, 12 patients (11.65%) developed SSI. We found a relationship between SSI development and high body mass index; the presence of diabetes mellitus, presence of transfusion, and high pre-operative glucose level. No relationship among age, hypertension, smoking, ASA score, presence of drainage, duration of operation, pre-operative hemoglobin level, and development of SSI has been determined. In conclusion, although there are patient-related and non-modifiable risk factors, we are in thought of that prediction of modifiable risk factors, such as blood glucose level, may reduce the frequency of SSI after endometrial cancer surgery.","PeriodicalId":399572,"journal":{"name":"Disease and Molecular Medicine","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129457020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1