Basic Gastronenterology最新文献

{"title":"IDDF2021-ABS-0199 Revealing molecular and cellular discriminants of type 2 diabetes by integrative analysis of pancreatic single-cell RNA-seq DATA","authors":"Tao Zeng, Hui Tang, Xiangtian Yu, Rui Liu","doi":"10.1136/gutjnl-2021-iddf.57","DOIUrl":"https://doi.org/10.1136/gutjnl-2021-iddf.57","url":null,"abstract":"C) accumulation in serum, thus decreased atherogenic index of plasma and arteriosclerosis index values. More importantly, Cana decreased the thickness of the vascular basement membrane, improved cardiac mitochondrial homeostasis, and relieved oxidative stress (e.g. regulation of ROS, SOD, GSH, and MDA levels). And, Cana reduced the circulating markers of inflammation (such as TNFa, MCP-1, and IL-6). Myocardial injury was alleviated after Cana treatment with decreasing levels of serous cTn I (from 95 pg/mL to 75 pg/mL) and sCD40L (from 120 pg/ mL to 90 pg/mL). Thus, the cardiovascular abnormality was relieved by elevating the CD31 expression level and suppressing fibrosis and basement membrane thickening (from 0.6 mm to 0.2 mm) in T2DM mice. Interestingly, Cana administration increased the ratio of Firmicutes/Bacteroidetes (from 230% to 98%) and the relative abundance of Olsenella, Alistipes, and Alloprevotella, while decreasing the abundance of Helicobacter and Mucispirillum in mice with diabetic CVD. Conclusions Cana treatment improved CVD by decreasing the risk of atherosclerosis and reducing the thickness of the vascular basement membrane. Importantly, Cana treatment significantly elevated myocardial mitochondria homeostasis, thus ameliorated the oxidative stress and inflammatory states. Moreover, Cana subtly altered microbiota composition in T2DM mice with CVD, which contributed to the improvement of CVD. Collectively, the improvements of myocardial mitochondrial and gut microbiota homeostasis, may represent an important mechanism underlying the cardiovascular benefits of Cana treatment.","PeriodicalId":399396,"journal":{"name":"Basic Gastronenterology","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114783412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IDDF2021-ABS-0197 Delayed intervention of agaropectin-derived oligosaccharides alleviate lipid accumulation by modulating intestinal flora homeostasis","authors":"Xuelian Wang, Hao Jiang, C. Chao, L. Guoyun, Guangli Yu","doi":"10.1136/gutjnl-2021-iddf.55","DOIUrl":"https://doi.org/10.1136/gutjnl-2021-iddf.55","url":null,"abstract":"IDDF2021-ABS-0196 Figure1B. Clostridium butyricum and its supernatant ameliorated colitis and regulated immune cells; The macroscopic images of colon)( IDDF2021-ABS-0196 Figure 1C. Clostridium butyricum and its supernatant ameliorated colitis and regulated immune cells; The statistical analysis of colon length). The results of qPCR showed that the relative expression of pro-inflammatory cytokines TNF-a, IL-6, IL-1b, IL-17a were down-regulated after CBM and its supernatant treatment. And the relative expressions of anti-inflammatory cytokines TGF-b, IL-10, IL-4, IL-5, IL-13 were up-regulated (IDDF2021-ABS-0196 Figure1F. Clostridium butyricum and its supernatant ameliorated colitis and regulated immune cells; The amount of Th1 cells based on flow cytometry, and its statistical analysis). Flow cytometry showed that the amount of neutrophils, macrophage, Th1, Th17 cells were down-regulated which were the pro-inflammatory immune cells in intestinal mucosa lamina propria after CBM and its supernatant treatment (IDDF2021-ABS-0196 Figure1D. Clostridium butyricum and its supernatant ameliorated colitis and regulated immune cells; The amount of neutrophil based on flow cytometry, and its statistical analysis) (IDDF2021-ABS-0196 Figure1E. Clostridium butyricum and its supernatant ameliorated colitis and regulated immune cells; The amount of macrophage based on flow cytometry, and its statistical analysis) (IDDF2021-ABS-0196 Figure1F. Clostridium butyricum and its supernatant ameliorated colitis and regulated immune cells; The amount of Th1 cells based on flow cytometry, and its statistical analysis) (IDDF2021-ABS-0196 Figure1G. Clostridium butyricum and its supernatant ameliorated colitis and regulated immune cells; The amount of Th17 cells based on flow cytometry, and its statistical analysis) (IDDF2021-ABS0196 Figure1H. Clostridium butyricum and its supernatant ameliorated colitis and regulated immune cells; The relative expression of inflammatory cytokines) Conclusions CBM can alleviate the symptoms and colon shortening of mice with colitis after pre-treatment with ABx, improve the expression of inflammatory factors, and might play an anti-inflammatory role by down-regulating the infiltration of inflammatory immune cells in intestinal mucosa lamina propria.","PeriodicalId":399396,"journal":{"name":"Basic Gastronenterology","volume":"108 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115165779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IDDF2021-ABS-0074 Gut microbial metabolite of tryptophan retards intestinal stem cell maintenance under psychological stress in mice","authors":"Xiao Zheng, Yali Liu","doi":"10.1136/gutjnl-2021-iddf.31","DOIUrl":"https://doi.org/10.1136/gutjnl-2021-iddf.31","url":null,"abstract":"IDDF2021-ABS-0074 Figure 2 Abstract IDDF2021-ABS-0074 Figure 1 Abstracts A28 Gut 2021;70(Suppl 2):A1–A150 on D ecem er 5, 2021 by gest. P rocted by coright. http/gut.bm jcom / G t: frst pulished as 10.113utjnl-2021-ID D F 31 on 2 S etem er 221. D ow nladed fom","PeriodicalId":399396,"journal":{"name":"Basic Gastronenterology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123146988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IDDF2021-ABS-0108 Enterobacteriaceae – deciphering the culprit gut bacteria causing necrotizing enterocolitis in infants","authors":"Jodi Woan‐Fei Law, V. Letchumanan, Hooi‐Leng Ser, Loh Teng-Hern Tan, Priyia Pusparajah, Learn‐Han Lee","doi":"10.1136/gutjnl-2021-iddf.39","DOIUrl":"https://doi.org/10.1136/gutjnl-2021-iddf.39","url":null,"abstract":"level or without experimental intervention (e.g., reviews) were excluded. Results Twenty-one studies were identified for qualitative analysis, which amounted to 361 NEC cases. The majority of NEC cases were diagnosed in preterm infants, and the gut microbiome was determined by analyzing fecal/intestinal tract samples using microbial culture or culture-independent molecular techniques. The main findings demonstrated a significant increase in abundance of Enterobacteriaceae colonizing the gut of NEC infants than those of controls. Klebsiella spp. (Klebsiella pneumoniae, Klebsiella oxytoca), Shigella dysenteriae, Escherichia coli, and Citrobacter koseri were identified as the dominating taxa present in the gut of NEC infants (IDDF2021-ABS-0108 Figure 1. Enterobacteriaceae colonizing the gut of NEC infants). However, some studies reported contradicting results due to the inability to pinpoint clear signatures of gut microbial composition causing NEC. Dysbiosis of the gut microbiota caused by a higher abundance of Enterobacteriaceae can be a factor triggering an inflammatory response in premature infants. Preterm infants have immature intestinal host defenses that could expose them to a higher risk of intestinal diseases. Conclusions The outcome of this systematic review revealed an important trend of increased abundance of intestinal Enterobacteriaceae in NEC. Nevertheless, the role of Enterobacteriaceae in the development of NEC warrants further investigation. A better understanding of NEC is crucial for effective prophylactic and therapeutic interventions. Abstract IDDF2021-ABS-0108 Figure 1 AbstractsIDDF2021-ABS-0108 Figure 1 Abstracts A40 Gut 2021;70(Suppl 2):A1–A150 on D ecem er 2, 2021 by gest. P rocted by coright. http/gut.bm jcom / G t: frst pulished as 10.113utjnl-2021-ID D F 39 on 2 S etem er 221. D ow nladed fom","PeriodicalId":399396,"journal":{"name":"Basic Gastronenterology","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123768348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IDDF2021-ABS-0178 Association of coffee intake with reduced cancer-related mortality among diagnosed patients with colorectal cancer: a systematic review and meta-analysis","authors":"N. Raymundo, J. Dumagpi, C. Francisco","doi":"10.1136/gutjnl-2021-iddf.50","DOIUrl":"https://doi.org/10.1136/gutjnl-2021-iddf.50","url":null,"abstract":"","PeriodicalId":399396,"journal":{"name":"Basic Gastronenterology","volume":"92 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121171275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IDDF2021-ABS-0123 Streptomyces pluripotens MUSC 135T as a treasure trove for anti-colon cancer and anti-MRSA agents","authors":"Learn‐Han Lee, Hooi‐Leng Ser, Jodi Woan‐Fei Law, V. Letchumanan, Kok-Gan Chan, B. Goh, N. A. Mutalib, Loh Teng-Hern Tan","doi":"10.1136/gutjnl-2021-iddf.41","DOIUrl":"https://doi.org/10.1136/gutjnl-2021-iddf.41","url":null,"abstract":"","PeriodicalId":399396,"journal":{"name":"Basic Gastronenterology","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131842798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IDDF2021-ABS-0013 The method for obtaining a reproducible model of tuberculous peritonitis in rabbits","authors":"D. Plotkin, T. Vinogradova, M. Reshetnikov, M. Sinitsyn, S. Okovityi","doi":"10.1136/gutjnl-2021-iddf.21","DOIUrl":"https://doi.org/10.1136/gutjnl-2021-iddf.21","url":null,"abstract":"IDDF2021-ABS-0013 Figure 2 Abstract IDDF2021-ABS-0013 Figure 3 AbstractsIDDF2021-ABS-0013 Figure 3 Abstracts Gut 2021;70(Suppl 2):A1–A150 A15 on S etem er 1, 2021 by gest. P rocted by coright. http/gut.bm jcom / G t: frst pulished as 10.113utjnl-2021-ID D F 21 on 2 S etem er 221. D ow nladed fom","PeriodicalId":399396,"journal":{"name":"Basic Gastronenterology","volume":"347 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116714470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IDDF2021-ABS-0196 The effect and immune cell analysis of clostridium butyricum on dextran sulphate sodium induced colitis in mice pretreated with antibiotic cocktail","authors":"Jing Xu, Haoming Xu, Yao Peng, Hai-Lan Zhao, Yong Zhang, Xue Guo, Jiaqi Wang, Wen-qi Huang, Hongli Huang, Youlian Zhou, Y. Nie","doi":"10.1136/gutjnl-2021-iddf.54","DOIUrl":"https://doi.org/10.1136/gutjnl-2021-iddf.54","url":null,"abstract":"","PeriodicalId":399396,"journal":{"name":"Basic Gastronenterology","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123114266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IDDF2021-ABS-0150 Influence of ethnicity on the gut microbiota of singaporean and malaysian communities","authors":"J. Dwiyanto, K. Toh, J. Ho, T. Su, Jeremy Lim, Jonathan Wei Jie Lee, S. Rahman, C. Chong","doi":"10.1136/gutjnl-2021-iddf.46","DOIUrl":"https://doi.org/10.1136/gutjnl-2021-iddf.46","url":null,"abstract":"","PeriodicalId":399396,"journal":{"name":"Basic Gastronenterology","volume":"161 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115274702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IDDF2021-ABS-0072 P311 promotes M2 macrophage polarization and infiltration in pancreatic ductal adenocarcinoma","authors":"F. Duan, Muzi Ouyang, Dongwei Jia, K. Ma, Jian Pan","doi":"10.1136/gutjnl-2021-iddf.30","DOIUrl":"https://doi.org/10.1136/gutjnl-2021-iddf.30","url":null,"abstract":"","PeriodicalId":399396,"journal":{"name":"Basic Gastronenterology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130003547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}