IDDF2021-ABS-0197 Delayed intervention of agaropectin-derived oligosaccharides alleviate lipid accumulation by modulating intestinal flora homeostasis

Abstract

IDDF2021-ABS-0196 Figure1B. Clostridium butyricum and its supernatant ameliorated colitis and regulated immune cells; The macroscopic images of colon)( IDDF2021-ABS-0196 Figure 1C. Clostridium butyricum and its supernatant ameliorated colitis and regulated immune cells; The statistical analysis of colon length). The results of qPCR showed that the relative expression of pro-inflammatory cytokines TNF-a, IL-6, IL-1b, IL-17a were down-regulated after CBM and its supernatant treatment. And the relative expressions of anti-inflammatory cytokines TGF-b, IL-10, IL-4, IL-5, IL-13 were up-regulated (IDDF2021-ABS-0196 Figure1F. Clostridium butyricum and its supernatant ameliorated colitis and regulated immune cells; The amount of Th1 cells based on flow cytometry, and its statistical analysis). Flow cytometry showed that the amount of neutrophils, macrophage, Th1, Th17 cells were down-regulated which were the pro-inflammatory immune cells in intestinal mucosa lamina propria after CBM and its supernatant treatment (IDDF2021-ABS-0196 Figure1D. Clostridium butyricum and its supernatant ameliorated colitis and regulated immune cells; The amount of neutrophil based on flow cytometry, and its statistical analysis) (IDDF2021-ABS-0196 Figure1E. Clostridium butyricum and its supernatant ameliorated colitis and regulated immune cells; The amount of macrophage based on flow cytometry, and its statistical analysis) (IDDF2021-ABS-0196 Figure1F. Clostridium butyricum and its supernatant ameliorated colitis and regulated immune cells; The amount of Th1 cells based on flow cytometry, and its statistical analysis) (IDDF2021-ABS-0196 Figure1G. Clostridium butyricum and its supernatant ameliorated colitis and regulated immune cells; The amount of Th17 cells based on flow cytometry, and its statistical analysis) (IDDF2021-ABS0196 Figure1H. Clostridium butyricum and its supernatant ameliorated colitis and regulated immune cells; The relative expression of inflammatory cytokines) Conclusions CBM can alleviate the symptoms and colon shortening of mice with colitis after pre-treatment with ABx, improve the expression of inflammatory factors, and might play an anti-inflammatory role by down-regulating the infiltration of inflammatory immune cells in intestinal mucosa lamina propria.