Basic Gastronenterology最新文献

{"title":"IDDF2021-ABS-0106 Significance of the expression of iron death-related gene hsbp1 in esophageal squamous cell carcinoma","authors":"Mingxin Zhang, Ning Lu, Qian Li, M. Cui","doi":"10.1136/gutjnl-2021-iddf.38","DOIUrl":"https://doi.org/10.1136/gutjnl-2021-iddf.38","url":null,"abstract":"Background To analyze the prognosis and expression of the ferroptosis-related gene HSBP1 in esophageal squamous cell carcinoma and its possible regulatory mechanisms. Methods Screen ferroptosis-related genes from previously published literature and GSEA (Gene Set Enrichment Analysis) website. We used R 4.0.3 software to perform differential analysis on the expression and prognosis of ferroptosis-related genes, and to evaluate the immune score. Results A total of 30 ferroptosis-related genes were differentially expressed in esophageal squamous cell carcinoma (IDDF2021-ABS-0106 Figure 1, IDDF2021-ABS-0106 Figure 2). Prognostic analysis showed that only HSBP1 was associated with the prognosis of esophageal squamous cell carcinoma, and its high expression indicated a poor prognosis (IDDF2021-ABS-0106 Figure 3). In addition, univariate and multivariate COX regression analysis further proved that high expression of HSBP1 is an independent risk factor affecting prognosis (IDDF2021-ABS-0106 Table 1). GSEA enrichment analysis shows that high HSBP1 may be involved in the regulation of nucleotide excision and repair, spliceosome, proteasome and other pathways (IDDF2021-ABS-0106 Figure 4). The analysis of immune checkpoint results showed that patients with high HSBP1 expression had increased immune checkpoint molecules CLAG3 and TIGIT (IDDF2021-ABS-0106 Figure 5). Conclusions The iron death-related gene HSBP1 may be involved in the occurrence and development of esophageal squamous cell carcinoma and the regulation of immune checkpoints. This study provides a new research direction for future HSBP1-related esophageal squamous cell carcinoma research and treatment targets.","PeriodicalId":399396,"journal":{"name":"Basic Gastronenterology","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128349791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IDDF2021-ABS-0065 The tumor immunological characterizations of colon adenocarcinoma reveal clinically relevant population-specific differences","authors":"Midie Xu, Jinjia Chang, C. Tan, Xin Wang, Weiwei Weng, S. Ni, Meng Zhang, Lei Wang, Wen-hua Li, Dan Huang, W. Sheng","doi":"10.1136/gutjnl-2021-iddf.26","DOIUrl":"https://doi.org/10.1136/gutjnl-2021-iddf.26","url":null,"abstract":"Background Immunomodulation has become a topical area of interest in many tumors, being colon adenocarcinoma (COAD), not an exception. Considering the molecular heterogeneity of COADs and their non-immunogenic character, immunotherapy only showed a viable role in a certain subset of COADs. This study aimed to determine immune subtypes (ISs) of COADs for the selection of suitable patients from an extremely heterogeneous population. Methods Gene expression profiles and corresponding clinical information were collected from TCGA and GEO databases, respectively. Consensus clustering analysis was performed to identify the ISs. Immunogenomics methods were integrated to characterize the immune environment of each IS subtype. Linear discriminant analysis was conducted to establish an immune subtyping characteristic index (ISCI) for IS classification of COADs. Co-expression network analysis was used for detected hub genes. DAVID was used for functional annotation. Results Based on 17 prognostic immune characteristics, COADs were stratified into three ISs characterized by differential molecular, cellular and clinical features. Patients with the IS1 tumor had immune ‘hot’ and immunosuppressive phenotype, IS3 tumor had immune ‘hot’ phenotype, whereas those with the IS2 tumor had immune ‘cold’ phenotype. Patients with the IS1 tumor had the worst disease-free survival compared with the other IS types, while those with the IS3 tumor had the best prognosis. Moreover, the proportion of highest adenomatous polyposis coli (APC) mutations in IS2 subtype is significantly higher than that in the IS1 and IS3; the proportion of TP53 mutations in IS1 subtypes were significantly higher than IS2 and IS3; while the proportion of KRAS mutations in IS1 subtypes were significantly lower than IS2 and IS3. IS3 subtype is predicted more sensitive to Cisplatin than other ISs, while IS1 is predicted more sensitive to 5-FU. Furthermore, the ISCI developed based on immune subtypes of COADs revealed immune infiltration degree in individual patients and can be utilized to determine the IS of patients with COAD. Based on 17 prognostic immune characteristics, COADs were stratified into three ISs. Patients with the IS1 tumor had the worst disease-free survival compared with the other IS types, while those with the IS3 tumor had the best prognosis (IDDF2021-ABS-0065 Figure 1, IDDF2021-ABS-0065 Figure 2, IDDF2021-ABS-0065 Figure 3, IDDF2021-ABS-0065 Figure 4). The proportion of highest adenomatous polyposis coli (APC) mutations in IS2 subtype is significantly higher than that in the IS1 and IS3; the proportion of TP53 mutations in IS1 subtypes were significantly higher than IS2 and IS3; while the proportion of KRAS mutations in IS1 subtypes were significantly lower than IS2 and IS3 (IDDF2021-ABS-0065 Figure 5, IDDF2021-ABS-0065 Figure 6). Moreover, distinct molecular, cellular and clinical features were observed among different IS tumors: patients with the IS1 tumor had immune ‘hot’ and imm","PeriodicalId":399396,"journal":{"name":"Basic Gastronenterology","volume":"63 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123853869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IDDF2021-ABS-0166 Transplantation of stool from obese patients promotes intestinal tumorigenesis in mice","authors":"Xing Kang, Thomas N. Y. Kwong, Yunbi Ni, Yufeng Lin, Siu-Kin Ng, Joseph J.Y. Sung, S. H. Wong, Jun Yu","doi":"10.1136/gutjnl-2021-iddf.49","DOIUrl":"https://doi.org/10.1136/gutjnl-2021-iddf.49","url":null,"abstract":"","PeriodicalId":399396,"journal":{"name":"Basic Gastronenterology","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126456898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IDDF2021-ABS-0029 Glucose levels decrease after performing islamic fasting models but not only ramadan fasting in high-fat-high-fructose-induced rats","authors":"Naufal Arif Ismail, E.S.R. Kumala, M. Pramaningtyas","doi":"10.1136/gutjnl-2021-iddf.23","DOIUrl":"https://doi.org/10.1136/gutjnl-2021-iddf.23","url":null,"abstract":"IDDF2021-ABS-0029 Figure 1 Abstracts A16 Gut 2021;70(Suppl 2):A1–A150 on S etem er 4, 2021 by gest. P rocted by coright. http/gut.bm jcom / G t: frst pulished as 10.113utjnl-2021-ID D F 23 on 2 S etem er 221. D ow nladed fom","PeriodicalId":399396,"journal":{"name":"Basic Gastronenterology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130157513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IDDF2021-ABS-0165 Psychological well-being and sleep quality among healthy stool donors in singapore: a cross-sectional study","authors":"Tzi Shin Toh, J. Lee, K. Toh, J. Ho, J. Lim, A. Tan, C. Chong","doi":"10.1136/gutjnl-2021-iddf.48","DOIUrl":"https://doi.org/10.1136/gutjnl-2021-iddf.48","url":null,"abstract":"(TCAs), exhibit antibacterial activities. Hence, this systematic review explores the impact of antidepressants on gut microbiota and potential strategies to alleviate antidepressant-associated dysbiosis. Methods This systematic review was conducted based on the PRISMA guidelines. Predefined MeSH terms ‘antidepressants AND gut microbiome’; ‘antidepressants AND antimicrobial activity’ were used in three databases (Pubmed, Embase, ProQuest; from database inception to June 2021). Studies reporting on the gut microbiota variation and antidepressants action were included. Studies without antidepressants and/or gut microbiome data were excluded, including conference proceedings, reviews, systematic reviews, meta-analyses, and commentaries. Results According to the study’s inclusion criteria, twelve studies out of 300 articles were selected for the qualitative analysis. In the in-vivo studies, animals administrated with SSRIs had a decreased abundance of Firmicutes, Alphaproteobacteria, Bacteroides, Ruminococcus, Adlercreutzia, Turicibacter, and alpha diversity but an increase in Prevotella, Parabacteroides, Butyricimonas, and Alistipes. Besides, antidepressants were shown to exhibit significant in vitro antimicrobial activity against Akkermansia muciniphila, Bifidobacterium animalis, and Bacteroides fragilis, suggesting that the chronic use of antidepressants potentially causes adverse effects due to their antimicrobial effects and dysbiosis (IDDF2021-ABS-0164 Figure 1. Illustration of gut microbiota dysbiosis in response to antidepressants). Probiotics, prebiotics and fecal microbiota transplantation are shown to be promising strategies to ameliorate antidepressant-associated dysbiosis. Conclusions Understanding the interaction between antidepressants and gut microbiota, including dysbiosis as a consequence of treatment and potential side effects, is vital for the future development of better and personalized treatment. Supplementing the gut microbiome with prebiotics/probiotics could be an adjuvant treatment to improve the clinical efficacy of the current antidepressant therapies. IDDF2021-ABS-0165 PSYCHOLOGICAL WELL-BEING AND SLEEP QUALITY AMONG HEALTHY STOOL DONORS IN SINGAPORE: A CROSSSECTIONAL STUDY","PeriodicalId":399396,"journal":{"name":"Basic Gastronenterology","volume":"73 3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115825777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IDDF2021-ABS-0201 Study on the promotion of lipopolysaccharide from shewanella algae on the occurrence and development of colorectal adenoma","authors":"Jun-Xiao Zhou, Junyan Li, K. Liu, Yanxiao Liang, Hong Wang","doi":"10.1136/gutjnl-2021-iddf.59","DOIUrl":"https://doi.org/10.1136/gutjnl-2021-iddf.59","url":null,"abstract":"Background Colorectal adenoma (CRA) is a recognized precancerous lesion of colorectal cancer (CRC). Intestinal flora is closely related to many diseases, including CRA and CRC. Our previous study found that the abundance of Shewanella algae (S.algae) was significantly increased in CRA patients. Next, it was found that S.algae can significantly promote the development of CRA in APCmin/+ mice, and the levels of macrophages and lipopolysaccharide (LPS) were increased after using S.algae to gavage APCmin/+ mice. The bioactivity of LPS from different bacteria is different. Escherichia coli (E.coli) LPS has been shown to promote the development of tumors, while the bioactivity of S.algae LPS to CRA is not yet clear. Methods Twenty-four female C57BL/6J mice were randomly divided into the Control group, the AOM/DSS group, the AOM/DSS+LPS group, and the AOM/DSS+S.algae group, with 6 mice in each group. The mice in the three experimental groups were given an intraperitoneal injection of AOM 10mg/kg, while the Control group was injected with the same amount of normal saline. After a week, the mice in the three experimental groups were regrouped according to body weight and given 2.5% DSS solution for 5 days and normal drinking water for 14 days. The mice were weighed every 3 days and killed at the end of the experiment on the 26th day. S.algae and its LPS were administered began on the eighth day and was gavaged every two days until the end of the experiment, while the Control group were given an equal amount of PBS by gavage. Results Compared with the AOM/DSS group, mice in the AOM/DSS+LPS group and the AOM/DSS+S.algae group developed more intestinal polyps (IDDF2021-ABS-0201 Figure 1. Body mass, colorectal length, and polyps of mice in each group) and more severe atypical hyperplasia (IDDF2021-ABS-0201 Figure 2. The pathological changes of the colorectum in mice of each group). M1 macrophages (CD11b+/CD86+) (IDDF2021-ABS-0201 Figure 3. The protein and mRNA levels of CD11b, CD86, CD163 in adenoma tissue of each group), proliferation and periodic proteins (Ki67, PCNA, Cyclin D1) (IDDF2021-ABS-0205 Figure 4.The protein levels of Ki-67, PCNA, and Cyclin D1 in adenoma tissue of each group) and various pro-inflammatory factors (IDDF2021-ABS-0201 Figure 5. The expression of protein and mRNA of inflammatory factors in adenoma tissue of each group) were elevated, and TLR4/NF-κB pathway (IDDF2021-ABS-0201 Figure 6. The expression of TLR4/NF-κB pathway proteins in adenomas tissue of mice in each group) was activated after using S.algae and its LPS to gavage mice. Conclusions S.algae LPS may promote the occurrence and development of CRA in mice through M1 macrophages. S.algae LPS may promote the occurrence and development of CRA in mice through TLR4/NF-κB pathway.","PeriodicalId":399396,"journal":{"name":"Basic Gastronenterology","volume":"296 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130530379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IDDF2021-ABS-0132 Moving beyond the gastrointestinal tract: the involvement of gut microbiome in endometriosis","authors":"Hooi‐Leng Ser, J. Wong, V. Letchumanan, Jodi Woan‐Fei Law, Loh Teng-Hern Tan, Learn‐Han Lee","doi":"10.1136/gutjnl-2021-iddf.44","DOIUrl":"https://doi.org/10.1136/gutjnl-2021-iddf.44","url":null,"abstract":"","PeriodicalId":399396,"journal":{"name":"Basic Gastronenterology","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130654893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IDDF2021-ABS-0200 Bacillus amyloliquefaciens combined with resistant starch to ameliorate intestinal inflammation","authors":"Hai-Lan Zhao, Yao Peng, Jing Xu, Haoming Xu, Wen-qi Huang, Jiaqi Wang, Xue Guo, Yong Zhang, Hongli Huang, Youlian Zhou, Y. Nie","doi":"10.1136/gutjnl-2021-iddf.58","DOIUrl":"https://doi.org/10.1136/gutjnl-2021-iddf.58","url":null,"abstract":"","PeriodicalId":399396,"journal":{"name":"Basic Gastronenterology","volume":"18 3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130915699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IDDF2021-ABS-0099 Exploring the effects of acupuncture therapy in restoring health via modulation of intestinal microbiota","authors":"Loh Teng-Hern Tan, V. Letchumanan, Jodi Woan‐Fei Law, Hooi‐Leng Ser, B. Goh, Learn‐Han Lee","doi":"10.1136/gutjnl-2021-iddf.36","DOIUrl":"https://doi.org/10.1136/gutjnl-2021-iddf.36","url":null,"abstract":"Background Acupuncture has been an important complemen-tary and alternative therapy in China. It is a nonpharmaco-logic modality that involves penetrating the skin at specific points using the tips of thin stainless steel needles, with broad applications, safe, economical, convenient and few side effects. The procedure can be performed by manual manipulation or electrical stimulation (electroacupuncture (EA)). Recently, an increasing number of studies indicated that acupuncture/EA could modulate the gut microbiota composition, suggesting acupuncture/EA can treat diseases associated with intestinal microbial dysbiosis. Therefore, this study explores and sheds light on the existing evidence of the acupuncture/EA treatment in ameliorating various diseases by modulating intestinal microbiota composition. gene HSBP1 squamous cell regulatory mechanisms.","PeriodicalId":399396,"journal":{"name":"Basic Gastronenterology","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124778339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IDDF2021-ABS-0126 Exploring the gut microbiota variation in response to vibrio infection","authors":"Learn‐Han Lee, Ke-Yan Loo, Loh Teng-Hern Tan, Joanna Xuan Hui Goh, Woan-Fei Law, Hooi‐Leng Ser, Y. Kumari, D. Liew, V. Letchumanan","doi":"10.1136/gutjnl-2021-iddf.42","DOIUrl":"https://doi.org/10.1136/gutjnl-2021-iddf.42","url":null,"abstract":"","PeriodicalId":399396,"journal":{"name":"Basic Gastronenterology","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128461199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}