Molekulyarnaya Biologiya最新文献_第6页

[Development of a New Inhibitor of Bacterial Cystathionine γ-Lyase Based on 6-Bromoindole and Aminothiophene]. [基于6-溴吲哚和氨基噻吩的新型细菌胱硫氨酸γ-裂解酶抑制剂的研制]。

Molekulyarnaya Biologiya Pub Date : 2024-11-01 DOI: 10.31857/S0026898424060083, EDN: IAYTTU

R A Novikov, D N Platonov, A Yu Belyy, K V Potapov, M A Novikov, Yu V Tomilov, O I Kechko, T A Seregina, P N Solyev, V A Mitkevich

引用次数: 0

[Distribution of β-Lactamase-Producing Enterobacterales among Patients in Surgical and Therapeutic Departments of a Multidisciplinary Hospital]. [某多学科医院外科及治疗科产β-内酰胺酶肠杆菌的分布]。

Molekulyarnaya Biologiya Pub Date : 2024-11-01 DOI: 10.31857/S0026898424060115, EDN: IAJXDD

S Yu Meshchurova, A G Korobova, L M Samokhodskaya

{"title":"[Distribution of β-Lactamase-Producing Enterobacterales among Patients in Surgical and Therapeutic Departments of a Multidisciplinary Hospital].","authors":"S Yu Meshchurova, A G Korobova, L M Samokhodskaya","doi":"10.31857/S0026898424060115, EDN: IAJXDD","DOIUrl":"10.31857/S0026898424060115, EDN: IAJXDD","url":null,"abstract":"Adequate empiric therapy is difficult to choose without monitoring the local distribution of antibiotic-resistant bacteria in each hospital. The frequency of β-lactamase-producing Enterobacterales was compared in patients of therapeutic and surgical units. Antibiotic susceptibility was evaluated by the disk diffusion test. Production of extended-spectrum β-lactamases (ESBLs) was detected by the double-disk test, and carbapenemases were determined by a modified carbapenem inactivation method. Carbapenemase genes and their expression were quantified by real-time PCR and immunochromatography. More than one-third of Enterobacterales isolates produced ESBLs in both the therapeutic (35.51%) and surgical (39.85%) units. The proportions of carbapenemase producers was comparable in the two groups, amounting to 8.41 and 9.77%, respectively. Metallo-β-lactamases predominated in the surgical units; and serine β-lactamases, in the therapeutic units. β-Lactamase producers were less frequent among community-acquired isolates than among nosocomial ones in both therapeutic (31.48 and 56.6%) and surgical (45.45 and 51%) units, but the differences were nonsignificant. Although the proportion of β-lactamase producers in the surgical and therapeutic units was not found to increase over three years of the study, local monitoring should certainly be continued in order to develop a local strategy for adequate use of antibacterial drugs.","PeriodicalId":39818,"journal":{"name":"Molekulyarnaya Biologiya","volume":"58 6","pages":"1007-1019"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143459989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Antibiotic Resistance: Threats and Search for Solution]. [抗生素耐药性：威胁与解决方案的探索]。

Molekulyarnaya Biologiya Pub Date : 2024-11-01 DOI: 10.31857/S0026898424060012, EDN: HNDNRK

S N Kochetkov

引用次数: 0

[Antibiotic Resistance Genes in Cattle Gut Microbiota: Influence of Housing Conditions]. [牛肠道菌群抗生素耐药基因：饲养条件的影响]。

Molekulyarnaya Biologiya Pub Date : 2024-11-01 DOI: 10.31857/S0026898424060105, EDN: IALWAS

Sh A Begmatov, A V Beletsky, A L Rakitin, A P Lukina, L O Sokolyanskaya, A V Rakitin, L B Glukhova, A V Mardanov, O V Karnachuk, N V Ravin

{"title":"[Antibiotic Resistance Genes in Cattle Gut Microbiota: Influence of Housing Conditions].","authors":"Sh A Begmatov, A V Beletsky, A L Rakitin, A P Lukina, L O Sokolyanskaya, A V Rakitin, L B Glukhova, A V Mardanov, O V Karnachuk, N V Ravin","doi":"10.31857/S0026898424060105, EDN: IALWAS","DOIUrl":"10.31857/S0026898424060105, EDN: IALWAS","url":null,"abstract":"Resistance to antimicrobial drugs is an urgent problem not only in public health, but also in animal husbandry. The widespread use of antimicrobials in feed additives is one of the main reasons for the rapid spread of antibiotic resistance in the microbiota of the gastrointestinal tract of farm animals. To characterize antibiotic resistance genes (resistome), we performed metagenomic analysis of the feces of 24 cattle from different regions of Russia, including cows of different breeds and yaks. Animals differed in the type of housing: year-round on pastures or in barns of conventional farms, with consumption of feed additives. Although genes of resistance to aminoglycosides, β-lactams, glycopeptides, MLS antibiotics (macrolides, lincosamides, and streptogramins), phenicols, and tetracyclines were detected in samples from both groups of animals, the content of the resistome in the fecal microbiome of stall-bred cattle was about ten times higher than in animals kept on pastures. The resistome of stall cattle was dominated by β-lactamases and tetracycline resistance genes, the content of which in the microbiome was 24 and 60 times higher, respectively, than in animals kept on pastures. Apparently, the spread of resistance to β-lactams and tetracyclines in stall cattle reflects the active use of these antibiotics in livestock production. Metagenomic analysis of livestock feces can be used to quantify antibiotic resistance genes for the purpose of monitoring antimicrobial drugs used in animal husbandry.","PeriodicalId":39818,"journal":{"name":"Molekulyarnaya Biologiya","volume":"58 6","pages":"996-1006"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143459885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Distribution of Antibiotic Resistance Genes in Microbial Communities: The Impact of Anthropogenic Pollution]. [抗生素耐药基因在微生物群落中的分布：人为污染的影响]。

Molekulyarnaya Biologiya Pub Date : 2024-11-01 DOI: 10.31857/S0026898424060056, EDN: HMZHOY

I S Sazykin, M A Sazykina, A R Litsevich

{"title":"[Distribution of Antibiotic Resistance Genes in Microbial Communities: The Impact of Anthropogenic Pollution].","authors":"I S Sazykin, M A Sazykina, A R Litsevich","doi":"10.31857/S0026898424060056, EDN: HMZHOY","DOIUrl":"10.31857/S0026898424060056, EDN: HMZHOY","url":null,"abstract":"Issues related to the spread of antibiotic resistance genes in environmental microbial communities are considered. \"Hotspots\" of adaptive evolution, accumulation, and spread of antibiotic-resistant bacteria and genetic material of antibiotic resistance are highlighted. Such \"hotspots\" include anthropogenic ecosystems, such as municipal wastewater treatment plants, municipal solid waste landfills, livestock enterprises, and agrocenoses. The influence of various types of pollutants and biotic factors on enhancement of mutagenesis and horizontal transfer of antibiotic resistance genes is considered. The role of mobile genetic elements in mobilization and accelerated spread of resistance determinants is shown. Special attention is paid to the role of oxidative stress and stress regulons, which are activated for realization and control of molecular genetic mechanisms of adaptive evolution of bacteria and the horizontal distribution of genetic material in bacterial populations. Oxidative stress is identified as one of the main activators of genome destabilization and adaptive evolution of bacteria.","PeriodicalId":39818,"journal":{"name":"Molekulyarnaya Biologiya","volume":"58 6","pages":"937-952"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143459988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[History of the Creation of a New Generation of Antibiotics of the Group of Polycyclic Glycopeptides]. 【新一代多环糖肽类抗生素的发明史】。

Molekulyarnaya Biologiya Pub Date : 2024-11-01 DOI: 10.31857/S0026898424060029, EDN: HNBCOO

E N Olsufyeva

{"title":"[History of the Creation of a New Generation of Antibiotics of the Group of Polycyclic Glycopeptides].","authors":"E N Olsufyeva","doi":"10.31857/S0026898424060029, EDN: HNBCOO","DOIUrl":"10.31857/S0026898424060029, EDN: HNBCOO","url":null,"abstract":"Increased resistance to polycyclic glycopeptide antibiotics has become a serious problem for chemotherapy of infections caused by resistant Gram-positive bacteria. Chemical modification of known natural antibiotics is the main direction in the creation of the next generation of anti-infective drugs. Over the past two decades, a series of hydrophobic glycopeptide analogues active against resistant strains of Gram-positive bacteria have been developed, three of which - oritavancin, telavancin, and dalbavancin - were approved by the US Food and Drug Administration (FDA) in 2013-2014 for the treatment of infections caused by sensitive and resistant strains of staphylococci and enterococci. It has been established that hydrophobic derivatives of glycopeptides can act on resistant strains of bacteria by a mechanism that does not allow binding to the modified target of resistant bacteria. Understanding the mechanism of action of natural and modified glycopeptides is considered as the basis for the rational design of compounds with valuable properties to achieve fundamental results. The possibility of using semi-synthetic glycopeptide analogues in the fight against viral infections caused by enveloped viruses is also considered. This review outlines the main ways of chemical design in creating a new generation of glycopeptide antibiotics that overcome resistance to Gram-positive pathogens and the mechanisms of their action.","PeriodicalId":39818,"journal":{"name":"Molekulyarnaya Biologiya","volume":"58 6","pages":"862-886"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143460055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Toxicity Study of the Pharmacological Pair of Encapsulated Citrobacter freundii C115H Methionine γ-Lyase/Methiin]. 包膜弗氏柠檬酸杆菌C115H蛋氨酸γ-裂解酶/蛋氨酸药理学对的毒性研究

Molekulyarnaya Biologiya Pub Date : 2024-11-01 DOI: 10.31857/S0026898424060064, EDN: HMWQWG

S V Revtovich, V V Kulikova, V S Koval, A D Lyfenko, V A Kazakov, A S Chernov, G B Telegin, A S Zemskaya, N V Anufrieva, E A Morozova, P N Solyev

引用次数: 0

[Adapting Mouse Genome Editing Technique from Scratch Using in utero Electroporation]. [利用子宫电穿孔从零开始调整小鼠基因组编辑技术]。

Molekulyarnaya Biologiya Pub Date : 2024-11-01 DOI: 10.31857/S0026898424060142, EDN: IAETMS

J V Popova, V D Bets, E S Omelina, L V Boldyreva, E N Kozhevnikova

引用次数: 0

[New Epigenetic Markers of Age-Dependent Changes in the Cardiovascular System]. 心血管系统年龄依赖性变化的新表观遗传标记。

Molekulyarnaya Biologiya Pub Date : 2024-11-01 DOI: 10.31857/S0026898424060161, EDN: HZSYRB

L M Ermakova, E A Davydova, E V Kondakova, K V Kuchin, M V Vedunova

{"title":"[New Epigenetic Markers of Age-Dependent Changes in the Cardiovascular System].","authors":"L M Ermakova, E A Davydova, E V Kondakova, K V Kuchin, M V Vedunova","doi":"10.31857/S0026898424060161, EDN: HZSYRB","DOIUrl":"10.31857/S0026898424060161, EDN: HZSYRB","url":null,"abstract":"Cardiovascular diseases remain a predominant global cause of mortality, with a noteworthy rise in the risk of morbidity with advancing age. Besides, it accompanied by a phenomenon of disease rejuvenation in the circulatory system. Currently, epigenetic modifications play a key role in the genesis of cardiovascular diseases (CVD), influencing the complex interaction between genotype and phenotype variability. Consequently, delving into the realm of epigenetic markers offers a promising avenue to unravel the molecular underpinnings of cardiovascular disease pathogenesis. This study endeavors to pinpoint epigenetic markers intricately linked with age-related transformations in the cardiovascular system. The study revealed a robust correlation with age for two cardiological parameters: R wave tension in the augmented left arm lead (RaVL) and carotid-femoral pulse wave velocity (cfPWV). Moreover, these parameters exhibited a strong correlation with the DNA methylation level of 21 CpG-sites (CpGs) examined through the Illumina EPIC array. Notably, the majority of these identified CpG-sites are affiliated with genes involved in the development of pathologies of the cardiovascular system.","PeriodicalId":39818,"journal":{"name":"Molekulyarnaya Biologiya","volume":"58 6","pages":"1061-1074"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143460026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Expression Profiles of TRIM Family Genes in Neuronal and Glial Cell Cultures of Healthy Donors and Patients with Parkinson's Disease under Normal Conditions and Upon Neuroinflammation]. [TRIM家族基因在健康供体和帕金森病患者神经元和胶质细胞培养物中正常状态和神经炎症时的表达谱]。

Molekulyarnaya Biologiya Pub Date : 2024-11-01 DOI: 10.31857/S0026898424060172, EDN: HZSRSN

V V Nenasheva, E V Novosadova, T P Gerasimova, L V Novosadova, A Y Kotok, E L Arsenieva, E A Stepanenko, I A Grivennikov, V Z Tarantul

{"title":"[Expression Profiles of TRIM Family Genes in Neuronal and Glial Cell Cultures of Healthy Donors and Patients with Parkinson's Disease under Normal Conditions and Upon Neuroinflammation].","authors":"V V Nenasheva, E V Novosadova, T P Gerasimova, L V Novosadova, A Y Kotok, E L Arsenieva, E A Stepanenko, I A Grivennikov, V Z Tarantul","doi":"10.31857/S0026898424060172, EDN: HZSRSN","DOIUrl":"10.31857/S0026898424060172, EDN: HZSRSN","url":null,"abstract":"Proteins of the TRIM family are involved in both innate immunity and the nervous system processes and may play an important role in the development of neurodegenerative diseases. In this work, we analyzed the expression of 35 genes of the TRIM family in neural progenitors (NPs), terminally differentiated neurons (TDNs) and glial derivatives (NGs) obtained from induced pluripotent stem cells (iPSCs) of healthy donors (HD) and patients with Parkinson's disease (PD), in the absence of inflammatory stimuli and upon the induction of a nonspecific inflammatory response under the influence of TNFα. In NPs and TDNs of PD patients, compared with HD cells, differences in expression were observed for only a small number of TRIM genes. Under the influence of TNFα in TDNs, the expression of individual TRIM genes was activated, which was more significant in the cells of patients with PD compared to cells of HDs. In NGs of PD patients, the expression of many TRIM genes was initially reduced compared to HD cells and remained low or further decreased after exposure to TNFα. The data obtained demonstrate differences in the network of the TRIM family members in PD neurons and glia compared to control, and also show the multidirectional influence of the inflammatory stimulus on the expression of a number of TRIM genes in these types of cells. Considering the important role of many TRIM genes in the functioning of the innate immune system, it can be assumed that, in PD, more significant disturbances in the functioning of genes of this family occur in glia compared to neurons.","PeriodicalId":39818,"journal":{"name":"Molekulyarnaya Biologiya","volume":"58 6","pages":"1075-1086"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143460044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0