Hellenic Plant Protection Journal最新文献_第3页

Differentiation between Botryosphaeria dothidea and Neofusicoccum spp. based on a single nucleotide polymorphism in the ITS region 基于ITS区单核苷酸多态性的dothidea和Neofusicoccum sp .的区分

Hellenic Plant Protection Journal Pub Date : 2022-07-01 DOI: 10.2478/hppj-2022-0006

S. Palavouzis, A. Triantafyllopoulou, A. Tzima, E. Paplomatas

{"title":"Differentiation between Botryosphaeria dothidea and Neofusicoccum spp. based on a single nucleotide polymorphism in the ITS region","authors":"S. Palavouzis, A. Triantafyllopoulou, A. Tzima, E. Paplomatas","doi":"10.2478/hppj-2022-0006","DOIUrl":"https://doi.org/10.2478/hppj-2022-0006","url":null,"abstract":"Summary Fungi belonging to the Botryosphaeriaceae family are widespread pathogens of many angiosperms, causing disease on various high value crops. The most important members of the family for the Greek region and other Mediterranean countries are Botryosphaeria dothidea, Neofusicoccum hellenicum, Neofusicoccum mediterraneum and Neofusicoccum parvum. The frequently concurrent isolation of Botryosphaeriaceae species from the same host, as well as the extensive host range of B. dothidea, necessitate the development of rapid and reliable detection methods. This study presents a new and robust molecular diagnostic tool, in the form of a PCR method based on primers designed on an SNP (single nucleotide polymorphism) located in the ITS region (Internal Transcribed Region) of B. dothidea and Neofusicoccum species. SNP primers constructed with or without added mismatch nucleotides were combined with the same upstream universal primer to generate distinct amplicons. When evaluated in PCR assays, mismatched primers were found to have the highest differentiation capability. The potential for further development of SNP assays in order to differentiate between species is being evaluated.","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":"15 1","pages":"49 - 56"},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41581224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

First record of Sphenophorus placidus vestitus (Coleoptera: Curculionidae: Dryophthorinae) in Cyprus 文章标题塞浦路斯平斑蝶的首次记录(鞘翅目:斑蝶科:斑蝶科)

Hellenic Plant Protection Journal Pub Date : 2022-07-01 DOI: 10.2478/hppj-2022-0008

E. Kakouris, C. Makris, J. Demetriou

引用次数: 0

First record of the Nearctic Ozognathus cornutus (LeConte, 1859) (Coleoptera: Ptinidae: Anobiinae) in Cyprus 塞浦路斯近北Ozognathus cornutus（LeConte，1859）（鞘翅目：Ptinidae:Anobiinae）的首次记录

Hellenic Plant Protection Journal Pub Date : 2022-07-01 DOI: 10.2478/hppj-2022-0009

J. Demetriou, G. Kakiopoulos, A. Martinou

引用次数: 1

Biological parameters of grape phylloxera, Daktulosphaira vitifoliae, on local grapevine varieties in central Syria – Implications on their susceptibility 叙利亚中部葡萄品种葡萄根瘤蚜的生物学参数及其易感性的影响

Hellenic Plant Protection Journal Pub Date : 2022-07-01 DOI: 10.2478/hppj-2022-0010

I. Idris, A. Asaad, K. Houssian, I. Kalefa

引用次数: 0

Efficacy of fungicide alternatives against late wilt disease of maize and their influence on plant morphogenesis and yield characters 不同杀菌剂对玉米晚疫病的防治效果及其对植株形态发生和产量性状的影响

Hellenic Plant Protection Journal Pub Date : 2022-07-01 DOI: 10.2478/hppj-2022-0007

M. Abdel-Kader, M. Khalil, N. El-Mougy

{"title":"Efficacy of fungicide alternatives against late wilt disease of maize and their influence on plant morphogenesis and yield characters","authors":"M. Abdel-Kader, M. Khalil, N. El-Mougy","doi":"10.2478/hppj-2022-0007","DOIUrl":"https://doi.org/10.2478/hppj-2022-0007","url":null,"abstract":"Summary Efficiency of various organic acids, organic salts, essential oils, algae (an extract of Chlorella vulgaris and a commercial product), and bioagents against Magnaporthiopsis maydis, causing maize late wilt disease, was evaluated in laboratory and field conditions. For the in vitro tests, isolated M. maydis field strains from Egypt were used. Additionally, in field experiments different application methods were tested for their efficacy throughout two successive growing seasons. Results showed maximum growth inhibition of M. maydis at different concentrations of salicylic acid, ascorbic acid, benzoic acid and humic acid as well as sodium benzoate, potassium sorbate, di-potassim phosphate and calcium choloride, in descending order. The essential oils of carnation, lemongrass and black seed followed a similar trend. The minimum pathogenic fungal growth was achieved when the pathogen was exposed to the antagonistic Trichoderma viride followed by Trichoderma harzianum, Bacillus subtilis and Pseudomonas fluorescens treatments. Under field conditions, the highest disease reduction was recorded after treatment with salicylic acid (all application methods), humic acid (all application methods), sodium benzoate (seed dressing), carnation oil (seed dressing or seed dressing +foliar spray) and the bioagents B. subtilis and P. fluorescens (soil drench). Overall, all treatments and all application methods led to significant lower disease incidence compared to the untreated control in both growing seasons. Additionally, all treatments achieved an enhancement of plant morpho-genesis and yield characters. The most effective combinations of compounds/bioagents and application methods can be considered for future use in IPM management of late wilt disease of maize.","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":"15 1","pages":"57 - 71"},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46929854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Late blight of potato: From the great Irish potato famine to the genomic era – An overview 马铃薯晚疫病：从爱尔兰马铃薯大饥荒到基因组时代——综述

Hellenic Plant Protection Journal Pub Date : 2022-01-01 DOI: 10.2478/hppj-2022-0001

A. Majeed, S. Siyar, S. Sami

{"title":"Late blight of potato: From the great Irish potato famine to the genomic era – An overview","authors":"A. Majeed, S. Siyar, S. Sami","doi":"10.2478/hppj-2022-0001","DOIUrl":"https://doi.org/10.2478/hppj-2022-0001","url":null,"abstract":"Summary Late blight of potato and tomato, one of the most widely reported diseases of plants, is a significant curb in global agriculture which poses severe problems in terms of yield and economic losses, and environmental pollution due to pesticides use. The disease is caused by Phytophthora infestans -an oomycete - which first drew the considerable attention of plant pathologists during the mid-1840s when the pathogen incited historic starvation in Ireland – the great Irish potato famine - as a consequence of substantial potato losses due to late blight disease. Since that period, late blight has triggered several epidemics of potato and tomato of profound intensity in different regions. Over the course, synthetic fungicides have been proved effective management practice for late blight control; nonetheless, the evolution of new genotypes with increased virulence to hosts and resistance to fungicides has been greatly regarded as an agricultural problem. Breakthroughs in genome sequencing of P. infestans and identification of resistance genes in some plants have opened ways for the development of resistant genotypes. However, there still exist numerous challenges to deal with this noxious pathogen. This review aims to highlight the historical significance of late blight disease, its chemical control strategies and associated challenges, and resistance breeding programs by employing genetic approaches.","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":"15 1","pages":"1 - 9"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44145085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Dissipation of spiroxamine residues in open field cucumber and dietary risk assessment 露天黄瓜中螺恶胺残留的消散及膳食风险评价

Hellenic Plant Protection Journal Pub Date : 2022-01-01 DOI: 10.2478/hppj-2022-0005

F. Malhat, C. Anagnostopoulos, S. El-Sayed, S. Shokr

引用次数: 1

Lethal and sublethal effects of several plant compounds compared to spiromesifen against Tetranychus turkestani 几种植物化合物与螺虫胺对土耳其叶螨的致死和亚致死效果比较

Hellenic Plant Protection Journal Pub Date : 2021-07-01 DOI: 10.2478/hppj-2021-0009

F. Sohrabi, M. Ziaee

{"title":"Lethal and sublethal effects of several plant compounds compared to spiromesifen against Tetranychus turkestani","authors":"F. Sohrabi, M. Ziaee","doi":"10.2478/hppj-2021-0009","DOIUrl":"https://doi.org/10.2478/hppj-2021-0009","url":null,"abstract":"Summary Tetranychus turkestani Ugarov and Nikolski is one of the main agricultural pests of southwestern Iran and some other tropical regions. In the present study, fumigant activity of three essential oils extracted from Rosmarinus officinalis L., Mentha longifolia L. and Eucalyptus globulus Labill., and contact toxicity of two biopesticides (Tondexir and Palizin) on T. turkestani mite females were investigated and compared with that of spiromesifen, a chemical acaricide. Also, sublethal effects of the tested compounds at 50% lethal concentration (LC50) were estimated on the biological parameters of T. turkestani. The LC50 values for E. globulus, R. officinalis and M. longifolia essential oils were 12.50, 11.52 and 4.00 μl/l air and for spiromesifen, Tondexir and Palizin were 10.98, 327.34 and 858.13 ppm, respectively. All tested compounds significantly reduced adult female longevity, equally to the chemical acaricide spiromesifen. Fecundity also decreased in all treatments and this reduction was even higher for plant essential oils than the other compounds. Palizin, E. globulus and M. longifolia significantly reduced the hatchability of T. turkestani eggs similarly to spiromesifen. According to the results, the tested plant compounds are effective against T. turkestani and may be applied as suitable alternatives to synthetic pesticides against this crop pest.","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":"14 1","pages":"89 - 98"},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44880503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Volatile organic compound profiling of Capsicum annuum var. longum grown under different concentrations of nitrogen 不同氮浓度下长辣椒挥发性有机物特征分析

Hellenic Plant Protection Journal Pub Date : 2021-07-01 DOI: 10.2478/hppj-2021-0008

Y. David, J. B. Ylagan, H. Gonzales, J. Chan, J. Mondragon, M. Tavera, M. Redillas

{"title":"Volatile organic compound profiling of Capsicum annuum var. longum grown under different concentrations of nitrogen","authors":"Y. David, J. B. Ylagan, H. Gonzales, J. Chan, J. Mondragon, M. Tavera, M. Redillas","doi":"10.2478/hppj-2021-0008","DOIUrl":"https://doi.org/10.2478/hppj-2021-0008","url":null,"abstract":"Summary Emission of volatile organic compounds (VOCs) in plants is triggered by several biotic and abiotic factors, such as nutrient deficiency, environmental stress, and pathogenic attacks. For instance, plants suffering from limited or excessive nitrogen (N) supply may experience internal stress which can ultimately lower their stability and immunity making them susceptible to infection and infestation. In this study, VOCs from Capsicum annuum var. longum (Solanaceae) exposed to nitrogen (1.8 g/L, 4.5 g/L, and 9 g/L urea) were extracted using a 100 μm Solid Phase Microextraction (SPME) fiber coated with polydimethylsiloxane (PDMS). Using Gas Chromatography-Mass Spectrometry (GC-MS), extracted VOCs from N-treated plants were identified as Butanoic acid, 3-hexenyl ester, (E)-; Butanoic acid, hexyl ester; Hexanoic acid, 3-hexenyl ester, (Z)-; Hexanoic acid, 4-hexen-1-yl ester; cis-3-Hexenyl cis-3-hexenoate and 4-Pentenoic acid 2-methyl-, hexyl ester. Among these volatiles, butanoic acid, 3-hexenyl ester showed the most distinctive peak from the N-treated plants in comparison with the untreated. In addition, the Green Leaf Volatiles (GLV) 3-Hexenal; 2-Hexenal; 3-Hexen-1-ol, (Z)-; 2-Hexen-1-ol, (E) and 1-Hexanol were also detected from the N-treated plants. The identification of plant volatiles provides useful information that can be used in agricultural practices and plant phenotyping.","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":"14 1","pages":"77 - 88"},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42876370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of the effects of household processing on the reduction rate of chlorpyrifos, metalaxyl and diazinon residues in orange fruit 家庭加工对柑桔果实中毒死蜱、甲螨灵和二嗪农残留量降低率的影响

Hellenic Plant Protection Journal Pub Date : 2021-07-01 DOI: 10.2478/hppj-2021-0007

E. El-Sayed, H. Hassan, A. El-Raouf, S. Salman

{"title":"Investigation of the effects of household processing on the reduction rate of chlorpyrifos, metalaxyl and diazinon residues in orange fruit","authors":"E. El-Sayed, H. Hassan, A. El-Raouf, S. Salman","doi":"10.2478/hppj-2021-0007","DOIUrl":"https://doi.org/10.2478/hppj-2021-0007","url":null,"abstract":"Summary The effect of the household processing on the reduction rate of chlorpyrifos, metalaxyl and diazinon residues in contaminated oranges has been investigated and the processing factors were determined. The evaluation included validation parameters, matrix effect (ME %), reduction behavior and processing factors (PFs). Validation parameters were successfully applied; the three pesticides showed satisfactory recovery (70–120%) and precision (relative standard deviation - RSD<20%); they also exhibited no matrix effect. The most effective process in the pesticide residues reduction was juicing, followed by pulping while the washing process was less efficient in removing all pesticide residues; sonication showed a high reduction rate with both chlorpyrifos and diazinon. The processing factors (PFs) were generally less than one which indicates that all processes can reduce pesticide residues in oranges. The results could guide the safe and reasonable use of chlorpyrifos, diazinon, and metalaxyl. These processes contribute substantially to reduce consumer exposure to pesticide residues in oranges.","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":"14 1","pages":"65 - 76"},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46870677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3