Hellenic Plant Protection Journal最新文献

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Repellent effect of Gardenia jasminoides ethanol extracted oil on Blattella germanica and Monomorium pharaonis 栀子乙醇提取油对德国小蠊和法老王的驱避作用
Hellenic Plant Protection Journal Pub Date : 2023-07-01 DOI: 10.2478/hppj-2023-0007
T. A. Wagan, C. Hamada, W. Cai, H. Hongxia, G. S. Solangi, R.H. Lyu
{"title":"Repellent effect of Gardenia jasminoides ethanol extracted oil on Blattella germanica and Monomorium pharaonis","authors":"T. A. Wagan, C. Hamada, W. Cai, H. Hongxia, G. S. Solangi, R.H. Lyu","doi":"10.2478/hppj-2023-0007","DOIUrl":"https://doi.org/10.2478/hppj-2023-0007","url":null,"abstract":"Summary The study examined the repellency of Gardenia jasminoides ethanol-extracted oil against the German cockroach, Blattella germanica, and the pharaoh ant, Monomorium pharaonis, which are serious pests in areas of public health hygiene. For the repellency tests, 31.4 μg of the oil was applied per cm2 on one half of filter paper discs (9 or 15 cm diameter for the ant and cockroach, respectively), whereas the other half was treated as control (DMSO + Tween). Repellency effects were observed 1, 2, 3 and 4 h after the insect release. The oil showed high repellency against all life stages of cockroaches and worker ants. The maximum repellency was observed for the cockroach adults (81.7 ± 3.1%) followed by the fourth, third and second nymphal stages (76.7 ± 4.2, 75.0 ± 3.4, and 56.7 ± 8.4%, respectively), after 1h exposure. The repellence effect was strong against worker ants (78.3 ± 4.8%) after 1 h exposure. The repellence effect can last at least four hours for both species. Analysis of Gardenia oil with Gas chromatography-mass spectrometry identified 14 major chemical components.","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":"16 1","pages":"50 - 58"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42308033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Behavioral and histopathological changes of Clarias gariepinus as a predatory fish against Culex pipiens larvae following exposure to sublethal concentration of quinclorac and bensulfuron-methyl based herbicide 在暴露于亚致死浓度的喹氯rac和苄磺隆甲基除草剂后,作为一种捕食性鱼类的加里平Clarias gariepinus对库蚊幼虫的行为和组织病理学变化
Hellenic Plant Protection Journal Pub Date : 2023-07-01 DOI: 10.2478/hppj-2023-0009
I. Mohamed, M. Fathy, A. Farghal, S. Temerak, sary Abd El-ghaffar, Shaimaa K. Idriss
{"title":"Behavioral and histopathological changes of Clarias gariepinus as a predatory fish against Culex pipiens larvae following exposure to sublethal concentration of quinclorac and bensulfuron-methyl based herbicide","authors":"I. Mohamed, M. Fathy, A. Farghal, S. Temerak, sary Abd El-ghaffar, Shaimaa K. Idriss","doi":"10.2478/hppj-2023-0009","DOIUrl":"https://doi.org/10.2478/hppj-2023-0009","url":null,"abstract":"Summary Clarias gariepinus is one of the widespread culturable freshwater fish species in Africa, which is prevalent in various natural and human-made aquatic habitats including rice-fish system. This fish species displays predation potential on the aquatic stages of mosquitoes. Bensulfuron-methyl and quinclorac are herbicide active substances that have been extensively applied in rice culture in Egypt and other countries worldwide. This study assessed the adverse effects of sublethal concentration of a commercial herbicide formulation containing quinclorac and bensulfuron-methyl on the predation potential of C. gariepinus female and male predatory fish on Culex pipiens mosquito larvae. Also, stomach and intestine histopathology of the treated fish was investigated. The exposure of C. gariepinus to sublethal concentration of quinclorac and bensulfuron-methyl based herbicide produced detrimental effects on prey consumption and histopathological changes in the stomach and intestine of the fish. The mosquito consumption by the treated female and male fish decreased significantly compared to the untreated fish of both sexes. The histological changes in the intestines were hyperplasia of the intestinal epithelium and goblet cells; edema of lamina propria and broad intestinal villi, and distortion in intestinal villi in comparison to control. The stomach histopathology changes were necrosis and sloughing of mucosal epithelium with severe damage of sub-mucosa. Thus, the tested herbicide at sublethal concentration on C. gariepinus decreased the prey consumption on mosquito larvae and caused histopathological alterations in the fish that may impair its digestive physiology. These findings suggest a threat of the tested herbicide to C. gariepinus survival and potential as a native successful biocontrol agent against Cx. pipiens larvae.","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":"16 1","pages":"67 - 78"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49517469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of exogenous abscisic acid on antioxidant system of salt tolerant and salt sensitive cotton cultivars 外源脱落酸对耐盐和盐敏感棉花抗氧化系统的影响
Hellenic Plant Protection Journal Pub Date : 2023-07-01 DOI: 10.2478/hppj-2023-0006
K. Kuldoshova, A. Akhunov, N. Khashimova, J. Ziyavitdinov
{"title":"Effects of exogenous abscisic acid on antioxidant system of salt tolerant and salt sensitive cotton cultivars","authors":"K. Kuldoshova, A. Akhunov, N. Khashimova, J. Ziyavitdinov","doi":"10.2478/hppj-2023-0006","DOIUrl":"https://doi.org/10.2478/hppj-2023-0006","url":null,"abstract":"Summary Salinity is one of the most imperative global problems that affect crop productivity on a large scale. Salinity impairs plant growth and development by imposing various stresses. Therefore it is vital to decode those stress factors and identify possible solutions to improve agriculture productivity. However, the adaptive mechanisms under saline conditions of glycophytes have not been studied. The present study was undertaken to determine the effects of exogenous abscisic acid (ABA) on salinity tolerance in cotton plants. Some patterns of resistance development were revealed on the seedlings of two cotton cultivars, a salt-tolerant (Gulistan) and a salt-sensitive one (C-4727). Moreover, the antioxidant potentials of these cultivars were compared. The activities of ascorbate peroxidase (APX), super-oxide dismutase (SOD), as well as the quantity of endogenous ABA, malondialdehyde (MDA), and free proline (Pro) were determined in control and post treatment. Our results demonstrate significant differences between the salt- tolerant and sensitive cotton seedlings in response to saline stress, i.e., high levels of Pro and endogenous ABA, but lower MDA concentrations, and higher activity of APX and SOD for the salt-tolerant cultivar, Gulistan, as compared to the salt stress-sensitive cultivar C-4727.","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":"16 1","pages":"40 - 49"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45255761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of nano-silica extracted from two different plant sources on survival and development of Phthorimaea opercullela (Zeller) larvae 从两种不同植物来源提取的纳米二氧化硅对小蠊幼虫生存和发育的影响
Hellenic Plant Protection Journal Pub Date : 2023-07-01 DOI: 10.2478/hppj-2023-0008
I. Idris, M. Naddaf, H. Harmalani, R. Alshater, R. Alsafadi
{"title":"Effect of nano-silica extracted from two different plant sources on survival and development of Phthorimaea opercullela (Zeller) larvae","authors":"I. Idris, M. Naddaf, H. Harmalani, R. Alshater, R. Alsafadi","doi":"10.2478/hppj-2023-0008","DOIUrl":"https://doi.org/10.2478/hppj-2023-0008","url":null,"abstract":"Summary The study examined the effect of nano-silica extracted from two different plant sources on the survival and development of the potato tuber moth, Phthorimaea opercullela. The silica powder was derived from two different agricultural byproducts, olive stones and corncobs. Characterization by X-ray diffraction revealed that the extracted powder has an amorphous silica phase. The nitrogen adsorption-desorption measurements revealed that both extracted and treated silica have mesoporous structure, with a specific surface area of around 300 m2/g and 270 m2/g for the silica derived from olive stones and corncobs, respectively. The silica nanoparticles (SiO2 NPs) prepared from the silica derived from olive stοnes showed higher larvae mortality, pupae weight, and larval and pupal developmental time, compared to the silica derived from corncombs. The results show that the nano-silica derived from agriculture byproducts can be as effective as the synthetic insecticide (deltamethrin) utilized in control of the potato tuber moth, with lower environmental impact in terms of preventing pesticide residue accumulation. In addition, the efficiency of SiO2 NPs applications depends on the source of the silica nanoparticles and the applied concentration to achieve the optimum results for the pest control.","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":"16 1","pages":"59 - 66"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41994463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
New data on the parasitization of Aleurothrixus floccosus (Maskell) (Hemiptera: Aleyrodidae) in Greece 文章标题希腊絮状粉虱(半翅目:粉虱科)寄生性的新资料
Hellenic Plant Protection Journal Pub Date : 2023-07-01 DOI: 10.2478/hppj-2023-0010
G. Stathas, N. Kavallieratos, L.N. Cheliotis, P. Skouras, M.V. Giakoumaki, P. Milonas
{"title":"New data on the parasitization of Aleurothrixus floccosus (Maskell) (Hemiptera: Aleyrodidae) in Greece","authors":"G. Stathas, N. Kavallieratos, L.N. Cheliotis, P. Skouras, M.V. Giakoumaki, P. Milonas","doi":"10.2478/hppj-2023-0010","DOIUrl":"https://doi.org/10.2478/hppj-2023-0010","url":null,"abstract":"Summary Signiphora flavella (Girault) (Hymenoptera: Signiphoridae) was recorded in 2022 as a new parasitoid species of the serious pest of citrus Aleurothrixus floccosus (Maskell) in Greece, in two different areas, i.e., the provinces of Laconia and Messinia. Previously, Signiphora flavella was recorded in Greece parasitizing Hemiberlesia rapax (Comstock) and H. lataniae (Signoret) in early 60s. It is mainly a parasitoid species of scale insects belonging to Diaspididae (Hemiptera: Coccomorpha), whiteflies, or a hyperparasitoid of aphelinids.","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":"16 1","pages":"79 - 82"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42024518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of in-vitro antifungal activity against Fusarium incarnatum of binary and ternary combinations of lemongrass, garlic and mustard oil-encapsulated lipid nanoemulsions 柠檬草、大蒜和芥菜油包封脂质纳米乳二元和三元组合体外抗褐变镰刀菌活性评价
Hellenic Plant Protection Journal Pub Date : 2023-01-01 DOI: 10.2478/hppj-2023-0001
Minh-Hiep Nguyen, Thi-Ngoc-Mai Tran
{"title":"Evaluation of in-vitro antifungal activity against Fusarium incarnatum of binary and ternary combinations of lemongrass, garlic and mustard oil-encapsulated lipid nanoemulsions","authors":"Minh-Hiep Nguyen, Thi-Ngoc-Mai Tran","doi":"10.2478/hppj-2023-0001","DOIUrl":"https://doi.org/10.2478/hppj-2023-0001","url":null,"abstract":"Summary Encapsulated lipid nanoemulsions (EO-LNs) from garlic oil, lemongrass oil and mustard oil were prepared by a combined method of homogenization and sonication with the aim to generate highly effective formulations against Fusarium incarnatum (laboratory bioassays). Their combined binary and ternary formulations (preparations by mixing an equal volume ratio of each EO-LNs) were also tested. The synergistic/additive/antagonistic antifungal effect of the EOs (under nanoform) in their combined formulations was determined using the SynergyFinder software with the Bliss independence model. Results revealed the synergistic effect of the combined binary and ternary formulations of garlic oil-encapsulated LNs (NaG), lemongrass oil-encapsulated LNs (NaL) and mustard oil-encapsulated LNs (NaM). Furthermore, the ternary combination, at the same concentration of each constituent EO, had higher antifungal activity than the binary combinations. Nonetheless, at 600 times dilution the NaLG (binary combination) inhibited 96% the mycelial growth of F. incarnatum, which was significantly higher than the efficacy of NaMLG (ternary combination) in the same dilution. This could be possibly attributed to the 1.5-time higher concentration of each constituent EO in the binary combination compared to that in the ternary formulation. In addition, NaLG, even at the high EO concentration of 0.4 g/L, did not show any phytotoxicity symptoms on lettuce plants.","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":"16 1","pages":"1 - 11"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45880090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Unraveling the role of endophytic fungi in barley salt-stress tolerance 内生真菌在大麦耐盐性中的作用
Hellenic Plant Protection Journal Pub Date : 2023-01-01 DOI: 10.2478/hppj-2023-0002
R. Kouadria, M. Bouzouina, B. Lotmani, S. Soualem
{"title":"Unraveling the role of endophytic fungi in barley salt-stress tolerance","authors":"R. Kouadria, M. Bouzouina, B. Lotmani, S. Soualem","doi":"10.2478/hppj-2023-0002","DOIUrl":"https://doi.org/10.2478/hppj-2023-0002","url":null,"abstract":"Summary Salinity is an agricultural and eco-environmental problem worldwide that decreases crop production. Endophytic fungi have been shown to improve plant tolerance to stressful conditions. The purpose of the paper is to examine the efficiency of Embellisia phragmospora, Fusarium equiseti and Fusarium graminearum to improve tolerance of pot-grown barley in greenhouse under different levels of soil salinity (2.5, 8 and 14dS/m) by estimating growth, relative water content, mineral nutrition, photosynthetic pigments biosynthesis, proline and sugar levels. Results showed that E. phragmospora increased barley emergence rate to 66.7% compared to 60% recorded by non-colonized barley under 14dS/m soil salinity. The tested endophytes increased barley root length, shoot and root dry weights under salt stress. Endophytic fungi reduced Na+ accumulation and improved K+ uptake in barely under salinity. Fusarium equiseti and F. graminearum-inoculated barley increased proline content under salinity. Fusarium graminearum-colonized barley showed the highest sugar content under salt stress. Our findings demonstrate the feasibility of endophytic fungi bio-inoculation in improvement of barley tolerance to salt stress, which qualify them to be a potent tool to provide substantial benefits to crops for sustainable agriculture.","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":"16 1","pages":"12 - 22"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47818562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of the antibacterial activity of essential oil of Laurus nobilis against Pseudomonas syringae pv. phaseolicola and potential biocidal action 月桂精油对丁香假单胞菌的抗菌活性评价。Pharolicola及其潜在的生物杀灭作用
Hellenic Plant Protection Journal Pub Date : 2023-01-01 DOI: 10.2478/hppj-2023-0005
S. Mamoucha, A. Prombona, A. Galeou
{"title":"Evaluation of the antibacterial activity of essential oil of Laurus nobilis against Pseudomonas syringae pv. phaseolicola and potential biocidal action","authors":"S. Mamoucha, A. Prombona, A. Galeou","doi":"10.2478/hppj-2023-0005","DOIUrl":"https://doi.org/10.2478/hppj-2023-0005","url":null,"abstract":"Summary With a continuously growing human population on our planet, the chemical burden of our environment is also growing. In addition, the resistance of microorganisms, like bacteria, to widely used chemicals is evident. Therefore, the application of bactericidal products that reduce the risks for development of resistance as well as the environment and human safety is of great benefit. In this work, we have screened the essential oil extracted from plants of Laurus nobilis L. (laurel) grown at the base of the Greek mountain Olympus for its antimicrobial activity against two strains of the Phaseolus vulgaris pathogen, Pseudomonas syringae pv. phaseolicola as well as human pathogenic bacteria (biocidal use). Our results, obtained with established methods, like Well diffusion and Disc diffusion assay, reveal that laurel essential oil is a very effective bacteriostatic and bactericidal agent. Importantly, the activity of laurel essential oil as growth inhibitor of the plant pathogen Pseudomonas syringae pv. phaseolicola is reported for the first time. This opens the field for more extended investigations regarding its use in crop protection. Additionally, the laurel essential oil tested showed significant antibacterial properties against several human pathogenic bacteria, namely Micrococcus luteus, Escherichia coli, Staphylococcus aureus and Bacillus subtilis.","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":"16 1","pages":"29 - 39"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47065551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
First record of the scale insect Stotzia ephedrae in Greece 希腊首次记录鳞虫麻黄
Hellenic Plant Protection Journal Pub Date : 2023-01-01 DOI: 10.2478/hppj-2023-0003
G. Stathas, E. Kartsonas, A. Darras
{"title":"First record of the scale insect Stotzia ephedrae in Greece","authors":"G. Stathas, E. Kartsonas, A. Darras","doi":"10.2478/hppj-2023-0003","DOIUrl":"https://doi.org/10.2478/hppj-2023-0003","url":null,"abstract":"Summary The scale insect Stotzia ephedrae (Newstead) (Hemiptera: Coccomorpha: Coccidae) was recorded for the first time in Greece οn 20 April 2021. It was found on Ephedra foeminea Forssk. (Ephedraceae: Gnetales) in Athens. From preliminary studies it was found that S. ephedrae is an oviparous, bi-parental species completing one generation per year.","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":"16 1","pages":"23 - 25"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46910237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
First record of the scale insect Lineaspis striata on Picea glauca “Conica” 灰皮云杉“圆锥形”上鳞虫条纹纹纹皮虫的首次记录
Hellenic Plant Protection Journal Pub Date : 2023-01-01 DOI: 10.2478/hppj-2023-0004
G. Stathas, A. Darras, E. Kartsonas
{"title":"First record of the scale insect Lineaspis striata on Picea glauca “Conica”","authors":"G. Stathas, A. Darras, E. Kartsonas","doi":"10.2478/hppj-2023-0004","DOIUrl":"https://doi.org/10.2478/hppj-2023-0004","url":null,"abstract":"Summary The scale insect Lineaspis striata (Newstead) (Hemiptera: Coccomorpha: Diaspididae) was recorded for the first time infesting a species of the family Pinaceae. Heavily infested ornamental shrubs of Picea glauca “Conica” by pre-ovipostiting and oviposting female adults of L. striata, were found in March 2018 and by a minor population consisting of pre-ovipositing female adults in February 2021, in Attica, Greece. The scale was settled on the base of the needles of the host plant.","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":"16 1","pages":"26 - 28"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44892695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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