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Japanese Journal of Clinical Chemistry最新文献

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Determination of Type IV Collgen Level in Serum with Monoclonal Antibodies and Its Application to Liver Diseases 单克隆抗体测定血清ⅳ型胶原水平及其在肝病中的应用
Japanese Journal of Clinical Chemistry Pub Date : 1989-12-30 DOI: 10.14921/JSCC1971B.18.4_184
J. Kino, T. Yoshida, C. Asamatsu, Yoshihisa Sato, Hiroko Cho, K. Shiba, S. Tomura, Toshihiko Hayashi
{"title":"Determination of Type IV Collgen Level in Serum with Monoclonal Antibodies and Its Application to Liver Diseases","authors":"J. Kino, T. Yoshida, C. Asamatsu, Yoshihisa Sato, Hiroko Cho, K. Shiba, S. Tomura, Toshihiko Hayashi","doi":"10.14921/JSCC1971B.18.4_184","DOIUrl":"https://doi.org/10.14921/JSCC1971B.18.4_184","url":null,"abstract":"Shiseido Basic Research Laboratories, 1050 Nippa-cho, Kohoku-ku, Yokohama, 223, Japan *Department of Clinical Biochemistry , Faculty of Medicine, Tokyo Medical and Dental University, 1-5-4, Yushima, Bunkyo-ku, Tokyo, 113, Japan **Department of Internal Medicine, Nakano General Hospital, 4-59-16 Chuo, Nakano-ku, Tokyo. 164, Japan ***Department of Chemistry, College of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo, 153, Japan","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66733977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A New Determination Method for Polyamines and Their Acetylated Derivatives Involving High Performance Liquid Chromatography with an Enzyme Reactor and an Electrochemical Detector 酶反应器-电化学检测器高效液相色谱法测定多胺及其乙酰化衍生物
Japanese Journal of Clinical Chemistry Pub Date : 1989-12-30 DOI: 10.14921/JSCC1971B.18.4_191
K. Hiramatsu, S. Kamei, K. Iwasaki
{"title":"A New Determination Method for Polyamines and Their Acetylated Derivatives Involving High Performance Liquid Chromatography with an Enzyme Reactor and an Electrochemical Detector","authors":"K. Hiramatsu, S. Kamei, K. Iwasaki","doi":"10.14921/JSCC1971B.18.4_191","DOIUrl":"https://doi.org/10.14921/JSCC1971B.18.4_191","url":null,"abstract":"A simple and sensitive high performance liquid chromatographic determination method for free polyamines and their acetylated derivatives was developed. Polyamines were separated on an octadecyl polymer column with isocratic elution. The separated polyamines were introduced into an enzyme reactor containing immobilized acylpolyamine amidohydrolase, amine oxidase (flavin-containing) and putrescine oxidase, in which they were deacetylated and oxidizld to generate hydrogen peroxide. The amount of hydrogen peroxide generated was then determined with an electrochemical detector. When a mixture of nine pure","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66733881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Determination of Pranoprofen in Serum by Automated Column-Switching High-Performance Liquid Chromatography 高效液相色谱法测定血清中丙诺洛芬的含量
Japanese Journal of Clinical Chemistry Pub Date : 1989-12-30 DOI: 10.14921/JSCC1971B.18.4_180
K. Hikida, Y. Inoue, N. Kojima, Y. Ohkura
{"title":"Determination of Pranoprofen in Serum by Automated Column-Switching High-Performance Liquid Chromatography","authors":"K. Hikida, Y. Inoue, N. Kojima, Y. Ohkura","doi":"10.14921/JSCC1971B.18.4_180","DOIUrl":"https://doi.org/10.14921/JSCC1971B.18.4_180","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66733918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Use of Lysine (B29)-Horseradish Peroxidase Labeled Porcine Insulin in Solid Phase Antibody Enzyme-Immunoassay of Human Insulin 赖氨酸(B29)-辣根过氧化物酶标记猪胰岛素在人胰岛素固相抗体酶免疫分析中的应用
Japanese Journal of Clinical Chemistry Pub Date : 1989-10-30 DOI: 10.14921/JSCC1971B.18.3_95
M. Nakayama, K. Zaitsu, Y. Ohkura
{"title":"Use of Lysine (B29)-Horseradish Peroxidase Labeled Porcine Insulin in Solid Phase Antibody Enzyme-Immunoassay of Human Insulin","authors":"M. Nakayama, K. Zaitsu, Y. Ohkura","doi":"10.14921/JSCC1971B.18.3_95","DOIUrl":"https://doi.org/10.14921/JSCC1971B.18.3_95","url":null,"abstract":"In competitive enzyme-immunoassays (EIAs) of insulin, mixtures of several forms of enzyme-labeled insulins prepared using glutaraldehyde1), maleimide2) or periodate 3,4) have been used . There is no detailed comparison of enzyme-labeled insulins with respect to the sensitivity of EIA of insulin. The enzyme-labeling site (s) and the number of enzyme molecules incorporated per insulin molecule may be important factors influencing the sensitivity of the EIA. Recently we have developed a method for the preparation of horseradish peroxidase (HRP ; EC 1. 11. 1. 7) -labeled porcine insulins (HRP-insulins ; GlyA1-HRPinsulin, LysB29-HRP-insulin and GlyA1, LysB29-diHRP-insulin)5) (Fig. 1). This paper surveys the immunoreactivity of the above HRP-insulins with a mouse anti-human insulin monoclonal antibody and assesses the sensitivity of each as competitors in solid-phase EIA of human insulin. Two EIA methods for human insnlin are examined : a rapid method by simultaneous addition of HRPinsulin and a highly sensitive method by delayed addition of HRP-insulin.","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66733693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Determination of Free Cholestanol and Cholesterol in Human Serum by High-Performance Liquid Chromatography with Fluorescence Detection 高效液相色谱-荧光检测法测定人血清中游离胆固醇和胆固醇
Japanese Journal of Clinical Chemistry Pub Date : 1989-10-30 DOI: 10.14921/JSCC1971B.18.3_100
T. Iwata, M. Yamaguchi, Masaru Nakamura, Y. Ohkura
{"title":"Determination of Free Cholestanol and Cholesterol in Human Serum by High-Performance Liquid Chromatography with Fluorescence Detection","authors":"T. Iwata, M. Yamaguchi, Masaru Nakamura, Y. Ohkura","doi":"10.14921/JSCC1971B.18.3_100","DOIUrl":"https://doi.org/10.14921/JSCC1971B.18.3_100","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66733669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Simultaneous Assay of Phenylalanine and Tyrosine 苯丙氨酸和酪氨酸的同时测定
Japanese Journal of Clinical Chemistry Pub Date : 1989-10-30 DOI: 10.14921/JSCC1971B.18.3_105
Masaki Terada, Machiko Komiyama, Junichi Minami
{"title":"Simultaneous Assay of Phenylalanine and Tyrosine","authors":"Masaki Terada, Machiko Komiyama, Junichi Minami","doi":"10.14921/JSCC1971B.18.3_105","DOIUrl":"https://doi.org/10.14921/JSCC1971B.18.3_105","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66733677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Preanalytical Considerations in Blood Gas Analysis Total Quality Issues for the Clinical Laboratory 临床实验室血气分析总质量问题的分析前考虑
Japanese Journal of Clinical Chemistry Pub Date : 1989-08-31 DOI: 10.14921/JSCC1971B.18.2_68
R. Moran
{"title":"Preanalytical Considerations in Blood Gas Analysis Total Quality Issues for the Clinical Laboratory","authors":"R. Moran","doi":"10.14921/JSCC1971B.18.2_68","DOIUrl":"https://doi.org/10.14921/JSCC1971B.18.2_68","url":null,"abstract":"As indicated at the outset of this report, the critical nature of the clinical requirements when blood gas testing is indicated, coupled with the labile nature of the sample and rapidly changing patient conditions makes it imperative that the analyst be aware of more than just the analysis it self Knowledge by the analyst, of the physiology of blood gas, the interpretation of basic blood gas results, the effects of the changing patient environment and the manner in which the sample is treated in a few short minutes from collection to reporting are equally important if one is to assure timely and clinically meaningful results.","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66733621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ketone Body Ratio in the Blood in Acute and Subacute Cholestatic Liver Injury 急性和亚急性胆汁淤积性肝损伤时血酮体比的变化
Japanese Journal of Clinical Chemistry Pub Date : 1989-08-31 DOI: 10.14921/JSCC1971B.18.2_63
Y. Aoki, Z. Ogawa, H. Itoh
{"title":"Ketone Body Ratio in the Blood in Acute and Subacute Cholestatic Liver Injury","authors":"Y. Aoki, Z. Ogawa, H. Itoh","doi":"10.14921/JSCC1971B.18.2_63","DOIUrl":"https://doi.org/10.14921/JSCC1971B.18.2_63","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66733613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Electron Spin Resonance Studies on Microsomal Membrane Fluidity in Cholestatic Liver Injury Induced with α-Naphthylisothiocyanate α-异硫氰酸萘致胆汁淤积性肝损伤微粒体膜流动性的电子自旋共振研究
Japanese Journal of Clinical Chemistry Pub Date : 1989-08-31 DOI: 10.14921/JSCC1971B.18.2_35
Y. Aoki, K. Mitsuta, M. Kohno, H. Itoh
{"title":"Electron Spin Resonance Studies on Microsomal Membrane Fluidity in Cholestatic Liver Injury Induced with α-Naphthylisothiocyanate","authors":"Y. Aoki, K. Mitsuta, M. Kohno, H. Itoh","doi":"10.14921/JSCC1971B.18.2_35","DOIUrl":"https://doi.org/10.14921/JSCC1971B.18.2_35","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66733567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Glycation and the Development of Diabetic Retinopathy 糖化与糖尿病视网膜病变的发展
Japanese Journal of Clinical Chemistry Pub Date : 1989-06-30 DOI: 10.14921/JSCC1971B.18.1_17
M. Oimomi, Y. Maeda, F. Hata, T. Nakamichi, S. Baba, T. Iga, Misao Yamamoto
{"title":"Glycation and the Development of Diabetic Retinopathy","authors":"M. Oimomi, Y. Maeda, F. Hata, T. Nakamichi, S. Baba, T. Iga, Misao Yamamoto","doi":"10.14921/JSCC1971B.18.1_17","DOIUrl":"https://doi.org/10.14921/JSCC1971B.18.1_17","url":null,"abstract":"The levels of advanced-stage products of the Maillard reaction in lens proteins are considered to reflect the sum of accumulated glycation over time, because there is little turnover of lens crystallins. Thus, we investigated the relationship between the degree of diabetic complications, i. e. diabetic retinopathy and levels of advanced-stage products accumulated in the lens proteins prior to the surgical resection. The level of advanced-stage products in the lens proteins increased with the progression of diabetic retinopathy. The differences among the level in the groups of each diabetic retinopathy were significant. These results suggest that glycation measured by advanced Maillard product in the lens protein plays an important role in the etiology of diabetic complications.","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66733538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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