A New Determination Method for Polyamines and Their Acetylated Derivatives Involving High Performance Liquid Chromatography with an Enzyme Reactor and an Electrochemical Detector

Abstract

A simple and sensitive high performance liquid chromatographic determination method for free polyamines and their acetylated derivatives was developed. Polyamines were separated on an octadecyl polymer column with isocratic elution. The separated polyamines were introduced into an enzyme reactor containing immobilized acylpolyamine amidohydrolase, amine oxidase (flavin-containing) and putrescine oxidase, in which they were deacetylated and oxidizld to generate hydrogen peroxide. The amount of hydrogen peroxide generated was then determined with an electrochemical detector. When a mixture of nine pure