Results and Problems in Cell Differentiation最新文献_第4页

StrainMapperJ: An Easy-to-Use Digital Image Correlation Toolkit for Exploring and Quantifying the Mechanics of Deforming Tissues. StrainMapperJ：一个易于使用的数字图像相关工具包，用于探索和量化变形组织的力学。

Results and Problems in Cell Differentiation Pub Date : 2026-01-01 DOI: 10.1007/978-3-032-19509-8_6

Lance A Davidson, Sommer Anjum, Jing Yang, Geneva Masak

{"title":"StrainMapperJ: An Easy-to-Use Digital Image Correlation Toolkit for Exploring and Quantifying the Mechanics of Deforming Tissues.","authors":"Lance A Davidson, Sommer Anjum, Jing Yang, Geneva Masak","doi":"10.1007/978-3-032-19509-8_6","DOIUrl":"10.1007/978-3-032-19509-8_6","url":null,"abstract":"Multicellular tissues are shaped and remodeled during development, organogenesis, wound healing, cancer, and regeneration, as well as during a broad range of diseases. Time-lapse sequences of tissue movements convey a wealth of information about the processes driving their self-assembly and mechanobiology. Here we present StrainMapperJ, a digital image correlation (DIC) tool and supporting macros for the widely used open-source image analysis package ImageJ. StrainMapperJ can produce a diverse set of graphics for mechanical data exploration. At its core, StrainMapperJ uses the ImageJ plugin bUnwarpJ to map deformations in one image to match a second. From these results, StrainMapperJ can generate 2D maps of mechanical strain in the image frame, principal engineering strain and orientation, area strains, synthetic movement trajectories, and vorticity. StrainMapperJ can generate a variety of graphical outputs for quantitative analysis that registers or maps mechanical information directly onto the source image sequence. Additionally, the algorithms used in StrainMapperJ can yield quantitative information from short time-lapse sequences, often only a few minutes in duration, reducing the need for long-term imaging. In addition to these macros, we provide guidance on preparing image datasets, troubleshooting the analysis pipeline, and interpreting StrainMapperJ-produced maps for hypothesis generation and testing.","PeriodicalId":39320,"journal":{"name":"Results and Problems in Cell Differentiation","volume":"78 ","pages":"129-147"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148139281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of Bioactive Natural Compounds to Promote Cell Proliferation and Migration in the Wound Healing Process. 生物活性天然化合物在伤口愈合过程中促进细胞增殖和迁移的应用。

Results and Problems in Cell Differentiation Pub Date : 2026-01-01 DOI: 10.1007/978-3-032-19509-8_16

Kamila Kozłowska-Tomczyk, Zuzanna Flis, Justyna Gogola-Mruk, Piotr Szatkowski, Edyta Molik, Anna Ptak

{"title":"Application of Bioactive Natural Compounds to Promote Cell Proliferation and Migration in the Wound Healing Process.","authors":"Kamila Kozłowska-Tomczyk, Zuzanna Flis, Justyna Gogola-Mruk, Piotr Szatkowski, Edyta Molik, Anna Ptak","doi":"10.1007/978-3-032-19509-8_16","DOIUrl":"10.1007/978-3-032-19509-8_16","url":null,"abstract":"Wound healing is a complex biological process that restores the integrity and strength of the skin. Keratinocytes and fibroblasts play pivotal roles in tissue repair through their interactions and signaling pathways. Keratinocytes proliferate to re-epithelialize the wound, while fibroblasts contribute to the formation of new extracellular matrix. Although cell proliferation is essential for wound healing, excessive proliferation can lead to scar formation. Therefore, strategies that balance proliferation and migration, along with proper mechanotransduction, are crucial for efficient wound repair. Sheep and camel milk contain numerous bioactive substances, such as insulin, lactoferrin, proline, and conjugated linoleic acid, that have demonstrated promising effects on wound healing. While direct evidence on skin cell proliferation and migration is limited, the properties of these bioactive substances suggest beneficial effects in these areas. Therefore, incorporating these milks into hydrogel dressings can promote wound healing. Moreover, improving the cell-material mechano-interaction in such hydrogels could lead to the development of novel regenerative medical therapies.","PeriodicalId":39320,"journal":{"name":"Results and Problems in Cell Differentiation","volume":"78 ","pages":"419-455"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148139283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrating Mechanical Loading, Mechanotransduction, and Biological Responses in Musculoskeletal Tissues Across the Lifespan: Regulation Influenced by Cells, Extracellular Matrix, and Sex. 在整个生命周期中整合肌肉骨骼组织的机械负荷、机械传导和生物反应：受细胞、细胞外基质和性别影响的调节。

Results and Problems in Cell Differentiation Pub Date : 2026-01-01 DOI: 10.1007/978-3-032-19509-8_13

David A Hart

{"title":"Integrating Mechanical Loading, Mechanotransduction, and Biological Responses in Musculoskeletal Tissues Across the Lifespan: Regulation Influenced by Cells, Extracellular Matrix, and Sex.","authors":"David A Hart","doi":"10.1007/978-3-032-19509-8_13","DOIUrl":"10.1007/978-3-032-19509-8_13","url":null,"abstract":"Development of cellular mechanisms to facilitate mechanosensing and mechanotransduction occurred very early in evolution during adaptation to the boundary conditions of Earth, in particular, gravity. Subsequently, with the evolution of increasingly complex organisms leading to Homo sapiens, multiple levels of biological regulatory control of mechanical loading were introduced for connective tissues of the musculoskeletal system. Integration of mechanosensing mechanisms with biological regulatory variables exhibits tissue-specific, lifespan-specific, sex specific features. A key integration component is the regulation of response to ground reaction forces. This chapter will discuss various features of this integration and its dynamic nature across the lifespan, and the critical role of the cell-extracellular matrix relationship, and how sex-specific variables appear to influence the functioning of connective tissues, particularly in females, where lifespan transitions (i.e., puberty, pregnancy and lactation, and menopause) appear to contribute to tissue responses to loading.","PeriodicalId":39320,"journal":{"name":"Results and Problems in Cell Differentiation","volume":"78 ","pages":"319-365"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148139291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellular and Molecular Aspects of Chronic Rejection. 慢性排斥反应的细胞和分子方面。

Results and Problems in Cell Differentiation Pub Date : 2026-01-01 DOI: 10.1007/978-3-032-07686-1_1

Zhongcheng Mei, Alexander Sasha Krupnick

引用次数: 0

Chronic Rejection in Facial Vascularized Composite Allotransplantation (fVCA). 面部血管化复合异体移植（fVCA）的慢性排斥反应。

Results and Problems in Cell Differentiation Pub Date : 2026-01-01 DOI: 10.1007/978-3-032-07686-1_10

Felix J Klimitz, Leonard Knoedler, Samuel Knoedler, Bohdan Pomahac, Martin Kauke-Navarro

{"title":"Chronic Rejection in Facial Vascularized Composite Allotransplantation (fVCA).","authors":"Felix J Klimitz, Leonard Knoedler, Samuel Knoedler, Bohdan Pomahac, Martin Kauke-Navarro","doi":"10.1007/978-3-032-07686-1_10","DOIUrl":"https://doi.org/10.1007/978-3-032-07686-1_10","url":null,"abstract":"Chronic rejection (CR) remains a major barrier to long-term success in facial vascularized composite allotransplantation (fVCA), particularly in facial transplants. Unlike acute rejection, CR is a gradual, progressive process marked by vasculopathy, fibrosis, and functional graft decline. Histopathologic features include intimal hyperplasia, dermal sclerosis, adnexal atrophy, and telangiectasia. Immune mechanisms driving CR involve persistent activation of Th1 and Th17 T cells, macrophages, and, to a lesser extent, B cells, which form tertiary lymphoid structures. Dysregulated cytokines and chemokines, such as IFN-γ, IL-17, and IL-6, perpetuate inflammation and fibrosis, whereas the downregulation of regulatory mediators like IL-10 impairs immune resolution. Chronic antigen exposure, complement activation, and the presence of tissue-resident memory T cells further sustain graft injury. Clinically, CR presents with tightening of facial tissue, pigmentary changes, pain, and impaired oral and facial function, which can sometimes progress to necrosis and graft failure. Diagnostic challenges persist due to heterogeneous presentation and a lack of standardized criteria. Early detection through protocol biopsies, mucosal sampling, and vascular imaging is essential. Future directions emphasize the need for molecular diagnostics, targeted immunomodulation, and antifibrotic therapies. A deeper understanding of CR pathophysiology is critical to improving graft longevity and patient quality of life in fVCA.","PeriodicalId":39320,"journal":{"name":"Results and Problems in Cell Differentiation","volume":"77 ","pages":"201-221"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145890397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Female Germline Cysts in Clitellate Annelids: Organization, Functioning, and Diversity. 锁骨环节动物的雌性生殖系囊肿：组织、功能和多样性。

Results and Problems in Cell Differentiation Pub Date : 2026-01-01 DOI: 10.1007/978-3-032-06766-1_11

Piotr Świątek, Anna Z Urbisz

{"title":"Female Germline Cysts in Clitellate Annelids: Organization, Functioning, and Diversity.","authors":"Piotr Świątek, Anna Z Urbisz","doi":"10.1007/978-3-032-06766-1_11","DOIUrl":"10.1007/978-3-032-06766-1_11","url":null,"abstract":"Germ cell precursors developing into functional gametes tend to form syncytial groups of cells known as cysts, clusters, or nests. Such cysts develop in the early stages of gametogenesis when gonial cells divide without full cytokineses, and cells (cystocytes) stay interconnected by relatively large cytoplasmic channels termed cytoplasmic (intercellular) bridges or ring canals. Such germline cysts occur during oogenesis in clitellate annelids (Clitellata). A distinctive feature of female cysts in clitellates is the presence of the cytophore, a shared anuclear cytoplasmic mass that occupies the cyst center. Each clustering cystocyte has one ring canal connecting it to the cytophore. This general pattern of cyst architecture is conserved in this group; however, the number of interconnected cells (from eight to 2500) and the shape and dimensions of the cytophore (reticular, ball-like, or tree-like) vary between taxa. This plasticity in cyst organization is usually conserved at the family/subfamily level and can, therefore, be a useful morphological/histological feature for phylogenetical considerations.This chapter presents the general aspects of cysts' organization and function. Moreover, we present several variants of cyst organization found in the main groups of Clitellata, such as microdriles, leech-like taxa, and megadriles. We also note the recent progress in cyst analyses and the resulting perspectives on cyst evolution in this group.","PeriodicalId":39320,"journal":{"name":"Results and Problems in Cell Differentiation","volume":"76 ","pages":"207-248"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145582258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trunk Muscle Differentiation and Growth in Vertebrates. 脊椎动物躯干肌肉的分化和生长。

Results and Problems in Cell Differentiation Pub Date : 2026-01-01 DOI: 10.1007/978-3-032-06766-1_5

Damian Lewandowski, Małgorzata Daczewska

引用次数: 0

Evo-devo and the Biodiversity Crisis. 进化与生物多样性危机。

Results and Problems in Cell Differentiation Pub Date : 2026-01-01 DOI: 10.1007/978-3-032-06766-1_3

Peter K Dearden

引用次数: 0

The Ring-Legged Earwig, Euborellia annulipes (Dermaptera, Insecta): A Promising Model to Study Development and Reproductive Strategies of Hemimetabolous Insects. 环腿土蜈蚣（皮翅目，昆虫科）：研究半代谢昆虫发育和生殖策略的一个有前途的模型。

Results and Problems in Cell Differentiation Pub Date : 2026-01-01 DOI: 10.1007/978-3-032-06766-1_10

Paula Irles, Yoelvis Sulbaran-Bracho, Andres F Sarrazin

引用次数: 0

Epigenetic Regulation of RUNX2 in Bone Mechanobiology. RUNX2在骨力学生物学中的表观遗传调控。

Results and Problems in Cell Differentiation Pub Date : 2026-01-01 DOI: 10.1007/978-3-032-19509-8_14

Maria A Katsianou, Antonios N Gargalionis, Athanasios G Papavassiliou, Efthimia K Basdra

{"title":"Epigenetic Regulation of RUNX2 in Bone Mechanobiology.","authors":"Maria A Katsianou, Antonios N Gargalionis, Athanasios G Papavassiliou, Efthimia K Basdra","doi":"10.1007/978-3-032-19509-8_14","DOIUrl":"10.1007/978-3-032-19509-8_14","url":null,"abstract":"Bone is a highly mechanosensitive tissue that adapts its function in response to mechanical cues. These cues are converted into biomechanical signals that regulate osteogenic gene expression through mechanotransduction. Bone homeostasis and the long-term bone adaptation to its mechanical environment depend on the proper integration of mechanical signals with transcriptional programs that regulate osteogenesis. Runt-related transcription factor 2 (RUNX2) is a pioneer transcription factor, a mechanosensitive molecule, which coordinates osteoblast differentiation and bone remodeling. RUNX2 activity in mechanically induced environments is subject to epigenetic regulation. Mechanical cues modulate RUNX2 through epigenetic mechanisms, including DNA methylation, histone modifications, chromatin remodeling, and non-coding RNAs. Proper epigenetic coordination ensures the stability of osteogenic gene expression. Any disruption of this mechanotransduction-epigenetic route leads to bone diseases such as osteoporosis, osteoarthritis, and dysfunctional fracture repair. Moreover, epigenetic reprogramming of RUNX2 is increasingly evident in bone tumors, particularly osteosarcoma, where aberrant RUNX2 activity is associated with cell proliferation, tumor progression, survival, metastasis, and loss of differentiation. This chapter examines current evidence on RUNX2 epigenetic regulation in response to mechanical forces, discusses how its orchestration relates to bone disease and bone tumor development, and underscores potential therapeutic opportunities that arise from targeting specific epigenetic mechanisms.","PeriodicalId":39320,"journal":{"name":"Results and Problems in Cell Differentiation","volume":"78 ","pages":"367-377"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148139245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0