Cellular Therapy and Transplantation最新文献

{"title":"Is there a Future for Haematopoietic Cell Transplants?","authors":"R. Gale","doi":"10.18620/CTT-1866-8836-2020-9-4-6-10","DOIUrl":"https://doi.org/10.18620/CTT-1866-8836-2020-9-4-6-10","url":null,"abstract":"Recent advances in anti-cancer chemotherapy and in targeted and immune therapies raise the question whether there is a future for haematopoietic cell transplants. I discuss their importance but in the end the magnitude of these improvements is modest. I point out the efficacy of immune therapy is predominately restricted to B-cell cancers and that many if not most successful immune therapy recipients eventually receive an allogeneic haematopoietic cell transplant, especially those with acute lymphoblastic leukaemia (ALL). I also discuss most allotransplants are done for cancers not treated with current immune therapy. Randomized trials show an autotransplant is better than new drugs in young persons with plasma cell myeloma. Considerable data indicate much of the efficacy of allotransplants results from a non-cancer-specific allogeneic effect not expected to operate with current immune therapies. Lastly, I discuss a role for donor-derived chimeric antigen receptor (CAR)-T-cells in persons relapsing after an allotransplant for B-cell cancers. The sum of these considerations suggest an ongoing role for haematopoietic cell transplants in diverse settings.","PeriodicalId":39111,"journal":{"name":"Cellular Therapy and Transplantation","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78134159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fanconi Anemia in the Czech Republic: role of HSCT and long-term follow-up","authors":"P. Sedláček, P. Keslová, P. Smisek, M. Suková, M. Malíková, Spiros Tavandzis, J. Čermák, J. Starý","doi":"10.18620/CTT-1866-8836-2020-9-4-48-52","DOIUrl":"https://doi.org/10.18620/CTT-1866-8836-2020-9-4-48-52","url":null,"abstract":"Petr Sedlacek, Petra Keslova, Petr Smisek, Martina Sukova, Marcela Malikova, Spiros Tavandzis, Jaroslav Cermak, Jan Stary Department of Pediatric Hematology and Oncology, 2nd Medical School at Charles University, University Hospital Motol, Prague, Czech Republic Fanconi Anemia in the Czech Republic: role of HSCT and long-term follow-up Cellular Therapy and Transplantation (CTT). Vol. 9, No. 4, 2020 doi: 10.18620/ctt-1866-8836-2020-9-4-48-52 Submitted: 09 November 2020, accepted: 04 December 2020","PeriodicalId":39111,"journal":{"name":"Cellular Therapy and Transplantation","volume":"111 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72509377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endothelial differentiation of Wharton’s Jelly-derived mesenchymal stem cells seeded on chitosan/hyaluronan multilayer films","authors":"Hana Dennaoui, E. Chouery, C. Harmoush","doi":"10.18620/CTT-1866-8836-2020-9-4-59-67","DOIUrl":"https://doi.org/10.18620/CTT-1866-8836-2020-9-4-59-67","url":null,"abstract":"CTT JOURNAL | VOLUME 9 | NUMBER 4 | NOVEMBER-DECEMBER 2020 Hana Dennaoui 1, Eliane Chouery 2, Chaza Harmoush 1 1 Laboratory of Applied Biotechnology: Biomolecules, Biotherapies and Bioprocesses, AZM Centre for Biotechnology Research and its Applications, Doctoral School of Science and Technology, Lebanese University, Tripoli, Lebanon 2 Medical Genetics Unit, Faculty of Medicine, Saint Joseph University (USJ), Mar Mikhaël, Beirut, Lebanon Endothelial differentiation of Wharton’s Jelly-derived mesenchymal stem cells seeded on chitosan/hyaluronan multilayer films Cellular Therapy and Transplantation (CTT). Vol. 9, No. 4, 2020 doi: 10.18620/ctt-1866-8836-2020-9-4-59-67 Submitted: 25 August 2020, accepted: 14 November 2020","PeriodicalId":39111,"journal":{"name":"Cellular Therapy and Transplantation","volume":"654 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73461979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphology of target drug delivery systems (CaCO3 vaterites covered with dextran sulfate) in rat muscular tissue","authors":"P. Popryadukhin, N. Sudareva, Оlga М. Suvorova, G. Yukina, Еlena G. Sukhorukova, N. Saprykina, Ilya A. Barsuk, O. Galibin, A. Vilesov","doi":"10.18620/CTT-1866-8836-2020-9-4-78-84","DOIUrl":"https://doi.org/10.18620/CTT-1866-8836-2020-9-4-78-84","url":null,"abstract":"CTT JOURNAL | VOLUME 9 | NUMBER 4 | NOVEMBER-DECEMBER 2020 Pavel V. Popryadukhin 1, Natalia N. Sudareva 1,2, Оlga М. Suvorova 1, Galina Yu. Yukina 2, Еlena G. Sukhorukova 2, Natalia N. Saprykina 1, Ilya A. Barsuk 3, Oleg V. Galibin 2, Aleksandr D. Vilesov 1,2 1 Institute of Macromolecular Compounds RAS, St. Petersburg, Russia 2 Pavlov University, St. Petersburg, Russia 3 S.M.Kirov Military Medical Academy, St. Petersburg, Russia Morphology of target drug delivery systems (CaCO3 vaterites covered with dextran sulfate) in rat muscular tissue Cellular Therapy and Transplantation (CTT). Vol. 9, No. 4, 2020 doi: 10.18620/ctt-1866-8836-2020-9-4-78-84 Submitted: 05 November 2020, accepted: 04 December 2020","PeriodicalId":39111,"journal":{"name":"Cellular Therapy and Transplantation","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81820740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Donor-specific anti-HLA antibodies detection by de facto crossmatch method in pediatric recipients before haploidentical hematopoetic stem cell transplantation","authors":"O. Paina, I. Pavlova, N. Ivanova, A. Alyanskiy, T. Bykova, L. Zubarovskaya, A. Kulagin, B. Afanasyev, St. Petersburg Russia Transfusiology","doi":"10.18620/CTT-1866-8836-2020-9-4-53-58","DOIUrl":"https://doi.org/10.18620/CTT-1866-8836-2020-9-4-53-58","url":null,"abstract":"Olesya V. Paina 1, Irina E. Pavlova 1,2, Natalia E. Ivanova 1, Alexander L. Alyanskiy 1, Tatiana A. Bykova 1, Ludmila S. Zubarovskaya 1, Alexander D. Kulagin 1, Boris V. Afanasyev 1 1 RM Gorbacheva Research Institute of Pediatric Oncology, Hematology and Transplantation, Pavlov University, St. Petersburg, Russia 2 Russian Research Institute of Hematology and Transfusiology, St. Petersburg, Russia Donor-specific anti-HLA antibodies detection by de facto crossmatch method in pediatric recipients before haploidentical hematopoetic stem cell transplantation Cellular Therapy and Transplantation (CTT). Vol. 9, No. 4, 2020 doi: 10.18620/ctt-1866-8836-2020-9-4-53-58 Submitted: 02 September 2020, accepted: 04 December 2020","PeriodicalId":39111,"journal":{"name":"Cellular Therapy and Transplantation","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87785621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Augmented reality technology for auricular reconstruction in the treatment of microtia","authors":"А. I. Yaremenko, A. Lysenko, E. A. Ivanova, O. Galibin","doi":"10.18620/ctt-1866-8836-2020-9-2-78-82","DOIUrl":"https://doi.org/10.18620/ctt-1866-8836-2020-9-2-78-82","url":null,"abstract":"Facial defects and deformations occupy a significant place in the practice of maxillofacial and dental surgeons. Nevertheless, maxillofacial surgery is developing rapidly and requires improvement of existing treatment methods, and introduction of new approaches to reconstructive surgery. Augmented reality is a promising direction of computer technology development which is actively used in medicine and education. Modern computer technology allows to create a 3D model of a lost organ and use it for preoperative planning, as well as apply a virtual model for intraoperative navigation. Recently, the method of augmented reality has been actively developed, when a virtual image of the zone of the surgical area or a dedicated organ is used, which is compared with its real prototype in static mode, or in real-time using computer devices. The benefits of using augmented reality technologies in reconstructive surgery is associated with preoperative virtual planning, simplification of the surgical intervention itself, as well as with a reduction in the risks of intra- and postoperative complications. The aim of our work was to study the opportunity of using the augmented reality technology in reconstructive surgery for microtia correction based on pre-operative computer simulation.\u0000\u0000At the preoperative stage, a photometric analysis of the patient was carried out, then a computer simulation of the missing auricle was performed. Using a 3D printer, a virtual model of the reconstructed auricle was obtained. The image in three-dimensional format was loaded into augmented reality glasses, which made it possible to project the shape and position of the simulated auricle to the area of the defect of the auricle when preparing for surgery. During the surgery, a marker was installed near the surgical field, in order to display the three-dimensional model in a destined position. During surgical intervention, an autogenous costal cartilage was taken, from which the auricle was formed using augmented reality approach and three-dimensional modeling. Subsequently, the graft was introduced to the formed bed in the area of the right ear auricle.\u0000\u0000The obtained 3D model of the auricle before the operation enabled planning of the forthcoming operation and determine the amount of autograft needed for reconstruction. Using the augmented reality glasses, the exact shape of the auricle is reproduced during the operation, and its proper position is assessed in relation to the healthy side. No complications were observed over the postoperative period.\u0000\u0000Virtual modelling of a lost or absent organ based on a preoperative examination provides important information about its spatial structure. Preoperative virtual planning allows you to predict the individual features of the operation, its difficult stages, to anticipate possible complications. The use of augmented reality technology during reconstructive surgery is a promising method requiring further development and improvement.","PeriodicalId":39111,"journal":{"name":"Cellular Therapy and Transplantation","volume":"1 2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83752571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rolf Neth and Russia","authors":"M. Belogurova, L. Zubarovskaya","doi":"10.18620/ctt-1866-8836-2020-9-2-85-88","DOIUrl":"https://doi.org/10.18620/ctt-1866-8836-2020-9-2-85-88","url":null,"abstract":"Rolf Neth was also eager to keep scientific borders open and established early connections with scientists from Eastern Europe, particularly the Soviet Union, during the cold war. After the Chernobyl accident (April 1986) a number of doctors and scientists (e.g., R. P. Gale and P. Terasaki) arrived to Moscow within several days, in order to assist Russian doctors by attempting bone marrow transplantation in severely irradiated patients. To study long-term effects of Chernobyl accident, the affected areas of Ukraine and Belarus required well-arranged medical surveillance which was established by international collaboration of European scientists. Professor Rolf Neth has undertaken great efforts to develop appropriate medical infrastructure in radioactively contaminated areas and to organize appropriate laboratory facilities, mostly, for pediatric healthcare","PeriodicalId":39111,"journal":{"name":"Cellular Therapy and Transplantation","volume":"107 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77672778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacteroides fragilis is a potential marker of effective microbiota transplantation in acute graft-versus-host disease treatment","authors":"O. Goloshchapov, E. Bakin, M. Kucher, O. Stanevich, M. Suvorova, V. Gostev, O. Glotov, Y. Eismont, D. Polev, Anastasia Yu. Lobenskaya, R. Klementeva, Maria O. Goloshchapova, L. Zubarovskaya, S. Sidorenko, A. Suvorov, I. Moiseev, A. Chukhlovin","doi":"10.18620/ctt-1866-8836-2020-9-2-47-59","DOIUrl":"https://doi.org/10.18620/ctt-1866-8836-2020-9-2-47-59","url":null,"abstract":"Summary\u0000Fecal microbiota transplantation (FMT), as any other medical procedure, requires standardization of results, approaches, monitoring of its dynamics and microbiota engraftment evaluation. The aim of the present study was to compare efficiency and results of PCR and 16S RNA-based sequencing in order to trace the dynamics of microbiota composition after FMT.\u0000\u0000Patients and methods\u0000The prospective, single-center study included 27 patients with acute intestinal and chronic (overlap syndrome) graft-versus-host disease (GvHD) developed after allogeneic hematopoietic stem cell transplantation (HSCT). FMT in 19 cases was performed, mostly, with ingestible capsules, eight placebo-treated patients were included into control group. Quantitative changes of different bacterial groups in fecal microbiota were assessed by means of real-time multiplex PCR, being compared with16S rRNA sequencing technique at the terms of D+3, D+16, D+30, D+60 and D+120 following FMT. Clinical response was determined by 4 scales evaluating intestinal syndrome and GvHD grade.\u0000\u0000Results\u0000When evaluating stool consistence according to Bristol scale as an index of GvHD therapy efficiency, we have observed complete clinical response by the D+120 after FMT in nine cases (47% with Bristol score of ≤4 points), and nine patients (47%) showed improved stool properties (>4 points). In the placebo group, complete or partial response was revealed, respectively, in one (13%), and four cases (50%) on the D+120. Multiplex PCR of fecal microbiota has shown a different time course in FMT- and placebo-treated patients, when compared to their initial (pre-FMT) levels. Total bacterial mass and copy numbers of distinct microbial species exhibited sufficient increase after FMT. Such shifts were demonstrable on D+30 for total microbial mass (p=0.002); Escherichia coli (p=0.001); Bacteroides fragilis group (p=0.05); Faecalibacterium prausnitzii (p=0.005). Meanwhile, the numbers Lactobacillus spp., and Bacteroides thetaiotaomicron, generally, were not changed over this time period. Moreover, in the control group (placebo) we have not found significant fecal microbiota changes against initial levels during 120 days monitoring period. Over 120 days of observation, we have also found some differences of the microbiota dynamics for the subgroups with complete response and partial/no response: Bifidobacterium spp. (р<0.047), E.coli (р<0.00047), B. fragilis group (p=5.6×10-5), F.prausnitzii (р<0.0062).\u0000\u0000Conclusions\u00001. Quantitative PCR of the major bacterial groups of gut microbiota, e.g., Bifidobacterium spp., E. coli, B. fragilis group, F. prausnitzii could be used as microbiological markers for evaluation of changing microbial landscape after FMT as a routine molecular biology technique. 2. The genocopy counts of B. fragilis group correlate with clinical response in the patients with intestinal GvHD after HSCT, either with, or without FMT procedure.","PeriodicalId":39111,"journal":{"name":"Cellular Therapy and Transplantation","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74255611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is there any role for corticosteroids in GvHD prophylaxis?","authors":"T. Ruutu","doi":"10.18620/ctt-1866-8836-2020-9-1-8-12","DOIUrl":"https://doi.org/10.18620/ctt-1866-8836-2020-9-1-8-12","url":null,"abstract":"Summary Corticosteroids have an established role as the fi rst-line treatment of graft -versus-host disease (GvHD), but their role in the prophylaxis of GvHD is less clear. At present, corticosteroids are included in the prophylaxis regimens only rarely. Studies of adding corticosteroid to the most widely used prophylactic regimen, cyclosporine A and a short course of methotrexate, have yielded confl icting results, possibly due to diff erences in the treatment schedule. In our earlier published randomized prospective study, the addition of methylprednisolone (MP) to cyclosporine and methotrexate resulted in a markedly reduced incidence of acute GvHD. No diff erence was seen in the survival. In long-term follow-up of this study, aft er a median follow-up of 24.5 years in living patients, we observed a marked late non-relapse mortality among the patients not given prophylactic MP, probably due to higher incidence of chronic GvHD in this study arm. At the end of the follow-up, 55% of the patients given MP in the prophylaxis were alive, compared with 20% in the control arm. Th ese fi ndings suggest that the role of corticosteroids in GvHD prophylaxis should be reevaluated.","PeriodicalId":39111,"journal":{"name":"Cellular Therapy and Transplantation","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76195091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gender factor in hematopoietic stem cell transplantation","authors":"A. Chukhlovin","doi":"10.18620/ctt-1866-8836-2020-9-1-13-21","DOIUrl":"https://doi.org/10.18620/ctt-1866-8836-2020-9-1-13-21","url":null,"abstract":"of studies concerning metabolic, immunological and other diff erences between males and caused by their diff erential hormonal and background. However, few studies are dedicated to sex-dependent diff erences in amounts of donor hematopoietic cells used for stem cell transplantation (HSCT), kinetics of cytostatic drugs used for conditioning treatment and immunosuppressors for GvHD prophylaxis, as well as diff erences in common posttransplant complications. Th e following differences signifi cant for evaluation of HSCT results may be derived from previous studies: (1) Higher counts of CD34+ cells in hematopoietic graft s from males compared to female donors; (2) Metabolism of cytostatic drug in females suggest a tendency for decreased clearance and higher modifi cation rates due to increased CYP3A activities, along with decreased drug effl ux from target cells, thus suggesting higher accumulation of active cytostatic metabolites in female patients; (3) More eff ective and stable humoral immune response in females compared to males could be translated into better anti-infectious response, along with higher risk of chronic GvHD in females aft er allo-HSCT; (4) Male patients with some hematological malignancies subjected to allo-HSCT are more prone to posttransplant relapses, however, confl icting data are reported; (5) Increased risk of acute GvHD in males exists in cases of allo-HSCT from female donors. Th e issue of graft -versus-leukemia eff ect in this setting still remains open. In sum, estrogen hormones seem to be to the most probable cause of gender diff erences in HSCT-associated risks. However, modifying role of sex steroids is not well studied, and it should vary, depending on the age of patients. Th erefore, real signifi cance of sex diff erences in HSCT deserves further extensive studies in large databases.","PeriodicalId":39111,"journal":{"name":"Cellular Therapy and Transplantation","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90108032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}