Journal of Conservative Dentistry最新文献

{"title":"Effect of remineralizing agent on laser and nonlaser bleached enamel surfaces subjected to erosion: An <i>in vitro</i> study.","authors":"Swapnil Vats, Dakshita Joy Sinha, Sarita Singh, Ruchi Rathi, Swati Jha, Isha Singh","doi":"10.4103/jcd.jcd_139_23","DOIUrl":"10.4103/jcd.jcd_139_23","url":null,"abstract":"<p><strong>Aim: </strong>Assessment of remineralizing agent's effect on laser and nonlaser bleached enamel surfaces subjected to erosion.</p><p><strong>Materials and methods: </strong>In this study, 80 extracted human permanent anteriors were collected and divided into 4 groups with 20 teeth per sample. Enamel specimen of 3 mm × 3 mm were made using polyvinyl chloride rings and acrylic and randomly divided into four groups. Then the specimens were subjected to initial microhardness test using Vicker's hardness tester (AVK-CO, Mitutoyo, Japan). Two indentations were placed at 100 mm from one another in the center of all the samples. Bleaching with laser and without laser using hydrogen peroxide was performed followed by remineralization in the groups (Groups 1a and 2a) and then were subjected to erosion. The final hardness was measured using the above method used for initial microhardness.</p><p><strong>Results: </strong>Microhardness in the remineralized groups showed least variation. The group in which laser bleaching was performed along with remineralization as compared with nonlaser bleaching group with remineralization showed improvement in microhardness but the data was not statistically significant. A significant difference was noted between the laser and remineralization group when compared with the groups in which no remineralization was done.</p><p><strong>Conclusion: </strong>After bleaching the enamel surface is more prone to erosion, so to improve the microhardness of bleached enamel a remineralizing agent should be used. A combination of diode laser bleaching and remineralizing agents leads to improved microhardness of the bleached enamel thus proving this combination to be efficacious.</p>","PeriodicalId":38892,"journal":{"name":"Journal of Conservative Dentistry","volume":"26 4","pages":"388-394"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10497080/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10262503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of immediate dentin sealing on the fracture strength of indirect overlay restorations using different types of luting agents (A comparative <i>in vitro</i> study).","authors":"Yousif Hussam Abdulsattar, Alaa Jawad Kadhim","doi":"10.4103/jcd.jcd_226_23","DOIUrl":"10.4103/jcd.jcd_226_23","url":null,"abstract":"<p><strong>Objective: </strong>This study evaluates the effects of immediate dentin sealing (IDS) on the fracture resistance of lithium disilicate overlays using three different types of resin-luting agents (preheated composite, dual-cure adhesive resin, and flowable composite).</p><p><strong>Materials and methods: </strong>Forty-eight maxillary first premolars of equal size were prepared using a butt joint preparation design. The teeth were separated into two primary groups, each with 24 teeth: Group DDS - delay dentin sealing (DDS) (non-IDS) teeth were not treated. Group IDS - dentin sealing was applied immediately after teeth preparations. Each group was subsequently separated into three separate subgroups of eight teeth. Subgroups DDS+Phc and IDS+Phc - cemented with preheated composite (Enamel plus HRi, Micerium, Italy), subgroups DDS+Dcrs and IDS+Dcrs - cemented with dual-cured resin cement (RelyX Ultimate, 3M ESPE, Germany), and subgroups DDS+Fc and IDS+Fc - cemented with flowable composite (Filtek Supreme Flowable, 3M ESPE, USA). The fracture resistance of each sample was evaluated using a test of a single load till failure, which was automatically recorded in Newton by a computer-controlled universal testing system.</p><p><strong>Results: </strong>The fracture resistance of the subgroup IDS+Phc was the highest mean value, in which the overlay was cemented with preheated composite (1954 N), and the lowest mean was noted in the subgroup DDS+Fc, by which the overlay cemented with flowable composite without IDS (887 N). All IDS subgroups had a high mean fracture load. Both the Bonferroni test and the one-way ANOVA test identified a significant difference between all groups of 0.05.</p><p><strong>Conclusion: </strong>In general, teeth with IDS were stronger than teeth without IDS. When the preheated composite is used as a luting agent improves overall fracture resistance, followed by resin cement and flowable composite, respectively. However, the result showed that the ceramic overlays with and without IDS are strong enough to withstand the normal mastication force. Overlays was failed in a more catastrophic, irreparable mode of fracture than the clinical situation.</p>","PeriodicalId":38892,"journal":{"name":"Journal of Conservative Dentistry","volume":"26 4","pages":"434-440"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10497084/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10264062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiofilm activity of ultrasonic and diode laser activated sodium hypochlorite, chitosan, and chlorhexidine: A confocal laser scanning microscopic <i>in vitro</i> study.","authors":"L Krishna Prasada, U Akshay Kumar Pai","doi":"10.4103/jcd.jcd_224_23","DOIUrl":"10.4103/jcd.jcd_224_23","url":null,"abstract":"<p><strong>Aim: </strong>The aim of this study was to assess and compare the effect of ultrasonic and diode laser-activated sodium hypochlorite (NaOCl), chitosan, and chlorhexidine (CHX) on the removal of <i>Enterococcus faecalis</i> biofilm adherent to the root canal using a confocal laser scanning microscope (CLSM).</p><p><strong>Materials and methods: </strong>Root canals in 112 single-rooted teeth were instrumented using a rotary Ni-Ti system. Biofilms of <i>E. faecalis</i> were generated based on an established protocol. Samples were randomly divided into three experimental (<i>n</i> = 28) and one control (<i>n</i> = 28) group based on the irrigation protocol employed and the three experimental groups were further subdivided into subgroups based on the activation protocol (subgroup A - ultrasonic activated and subgroup B - diode laser activated). The groups were Group 1 (control), Group 2 (3% NaOCl for 6 min; subgroup A - activated using a diode laser, subgroup B - ultrasonic activation), Group 3 (2% CHX for 6 min; subgroup A - activated using a diode laser, subgroup B - ultrasonic activation), and Group 4 (0.2% chitosan for 6 min; subgroup A - activated using a diode laser, subgroup B - ultrasonic activation. Confocal laser scanning microscopy was used to assess bacterial viability <i>in situ</i>. Data were analyzed by appropriate statistical analyses with <i>P</i> = 0.05.</p><p><strong>Results: </strong>All experimental irrigation protocols destroyed the biofilm in the root canal lumen. Within the dentinal tubules, all groups had a significantly higher percentage of dead bacteria than the saline control (<i>P</i> < 0.05). There was no significant difference between CHX activated with ultrasonics, CHX activated with a diode laser, chitosan activated with ultrasonics and chitosan activated with diode laser groups (<i>P</i> > 0.05), whereas NaOCl ultra and NaOCl diode groups brought about more bacterial reduction than other groups (<i>P</i> < 0.05). The mean effectiveness and the bacterial kill ability were seen highest for the NaOCl activated with the ultrasonics group. There was no significant difference between diode laser activation and ultrasonic activation in CHX activated with ultrasonics, CHX activated with a diode laser, chitosan activated with ultrasonics and chitosan activated with diode laser groups (<i>P</i> > 0.05), but there was a significant difference between diode laser and ultrasonic activation in NaOCl group. Ultrasonic activation of the NaOCl was more effective than diode activation in reducing E. feacalis biofilms (<i>P</i> < 0.05).</p><p><strong>Conclusions: </strong>The use of NaOCl with the activation by ultrasonics caused the greatest reduction of <i>E. faecalis</i>. Ultrasonic activation was found superior to diode laser activation in dentinal tubule disinfection.</p>","PeriodicalId":38892,"journal":{"name":"Journal of Conservative Dentistry","volume":"26 4","pages":"441-446"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10497091/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10608885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative assessment of the outcome of complete pulpotomy using mineral trioxide aggregate and Biodentine in mature permanent mandibular molars with symptomatic irreversible pulpitis: A randomized clinical trial with 18 months of follow-up.","authors":"Rakesh Singla, Vipula Laller, Gurdeep Singh Gill, Namita Jain, Tarun Kumar, Jaidev Singh Dhillon","doi":"10.4103/jcd.jcd_170_23","DOIUrl":"10.4103/jcd.jcd_170_23","url":null,"abstract":"<p><strong>Background: </strong>Mature permanent teeth with irreversible pulpitis have traditionally been managed with pulpectomy. With advancements in pulp biology and dental materials, many clinicians are using vital pulp therapies like pulpotomy to manage such teeth. The current study was conducted to help clinicians in making decisions about case selection and choice of material for such cases.</p><p><strong>Aim: </strong>This randomized clinical trial evaluated the outcome of complete pulpotomy, using mineral trioxide aggregate (MTA) and Biodentine, in permanent mandibular molars with symptomatic irreversible pulpitis (SIP).</p><p><strong>Materials and methods: </strong>Fifty patients with moderate-to-severe pain in mandibular molars with SIP were included in this prospective, parallel, single-blind clinical trial. Coronal pulp was completely removed and hemostasis was achieved with a cotton pellet moistened with 2.5% sodium hypochlorite. Subsequently, the radicular orifices were randomly covered with MTA or Biodentine. All teeth were permanently restored with composite restoration at the same appointment. Clinical evaluation was performed at 1 week, 3 months, 6 months, 12 months, and 18 months and radiographic evaluation was done after 6 months, 12 months, and 18 months. Mann-Whitney <i>U</i> and Chi-square tests were utilized for statistical analysis.</p><p><strong>Results: </strong>Success rates of MTA and Biodentine pulpotomy were 63.6% and 69.6%, respectively, with no significant difference between the two groups at any follow-up period (<i>P</i> > 0.05).</p><p><strong>Conclusion: </strong>There were no significant differences in complete pulpotomy success rates between MTA and Biodentine over 18 months in mandibular molars with SIP.</p>","PeriodicalId":38892,"journal":{"name":"Journal of Conservative Dentistry","volume":"26 4","pages":"402-408"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10497083/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10625945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation of the quality and homogeneity of different obturating systems using cone-beam computed tomography - An <i>in vitro</i> study.","authors":"Grusha Gupta, Munish Singla, Harleen Kaur, Litik Mittal, Saloni Gupta, Savrose Kaur","doi":"10.4103/jcd.jcd_229_23","DOIUrl":"10.4103/jcd.jcd_229_23","url":null,"abstract":"<p><strong>Aim: </strong>The aim of this study was to evaluate the homogeneity and quality of different obturating systems using cone-beam computed tomography (CBCT).</p><p><strong>Materials and methods: </strong>One hundred and twenty extracted maxillary and mandibular molars were included in this study which was decoronated first and then dissected into palatal and distal roots. After working length determination, all the roots were biomechanically prepared with ProTaper universal system. The prepared roots were evaluated using CBCT, and volumetric analysis was done. Samples were then randomly divided into three groups corresponding to the obturation method used - cold lateral compaction technique, single-cone technique, and BeeFill 2 in 1 system. After obturation, CBCT imaging and volumetric analyses were performed again to compare and analyze the amount of volume of obturation in different obturating techniques. To standardize the volume, pre-and postobturation images were superimposed. To avoid errors, for each section, measurements were repeated three times and the means were calculated.</p><p><strong>Statistical analysis used: </strong>The data were statistically analyzed using one-way analysis of variance, followed by post hoc test.</p><p><strong>Results: </strong>Results showed a statistically significant difference of the single cone with lateral compaction and the BeeFill system (<i>P</i> < 0.001). Maximum volume of obturating material was found in BeeFill at all levels - Coronal, middle, and apical, followed by cold lateral compaction and single-cone technique (<i>P</i> < 0.001).</p><p><strong>Conclusion: </strong>It could be concluded that the thermoplasticized obturating technique can be a better choice for obturation rather than the single cone and lateral compaction technique.</p>","PeriodicalId":38892,"journal":{"name":"Journal of Conservative Dentistry","volume":"26 4","pages":"414-419"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10497074/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10608883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of ethylenediaminetetraacetic acid, maleic acid, and fumaric acid on postendodontic treatment root fracture toughness - An <i>in vitro</i> study.","authors":"Aabha Gautam Dave, P Laxmish Mallya, N Vasudev Ballal, Ramya Shenoy","doi":"10.4103/jcd.jcd_252_23","DOIUrl":"10.4103/jcd.jcd_252_23","url":null,"abstract":"<p><strong>Aim: </strong>The aim of this study was to evaluate the effectiveness of ultrasonic activation of ethylenediaminetetraacetic acid (EDTA), maleic acid (MA), and fumaric acid (FA) in combination with sodium hypochlorite (NaOCl) on postendodontic treatment root fracture toughness.</p><p><strong>Materials and methods: </strong>Forty single-rooted mandibular premolars were sectioned below the cementoenamel junction and were kept on average 14 mm long. With the exception of eight randomly selected teeth acting as a negative control group (Group I/nonprepared), the rest were prepared till F3. Thirty-two specimens were randomly placed into four groups equally and then irrigated using the final irrigation protocols. Saline and NaOCl were used to irrigate Group II, 17% EDTA and NaOCl were used to irrigate Group III, 7% MA and NaOCl were used to irrigate Group IV, and 0.7% FA and NaOCl were used to irrigate Group V. The irrigants were ultrasonically activated in every group. The root canals were obturated with gutta-percha and AH Plus sealer. The roots were embedded along the long axis in the self-curing acrylic blocks along with polyvinyl siloxane, leaving the coronal 2 mm of the root exposed. The specimens were tested using a universal testing machine.</p><p><strong>Statistical analysis: </strong>The <i>Post hoc</i> Tamhane's and one-way ANOVA tests were applied to examine the data.</p><p><strong>Results: </strong>The result of the study revealed that 0.7% FA (598.09 N) provided better root fracture resistance than EDTA (428.07 N) and MA (511.87 N).</p><p><strong>Conclusion: </strong>The conclusion derived from the study was that 0.7% FA provided better fracture resistance of endodontically treated teeth when compared to 17% EDTA and 7% MA.</p>","PeriodicalId":38892,"journal":{"name":"Journal of Conservative Dentistry","volume":"26 4","pages":"453-457"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10497077/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10315947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does the presence of full-coverage crown interfere with removal of obturation material: A cone-beam computed tomography image segmentation volumetric analysis.","authors":"Abhishek Bhargava, Vineeta Nikhil","doi":"10.4103/jcd.jcd_300_23","DOIUrl":"10.4103/jcd.jcd_300_23","url":null,"abstract":"<p><strong>Aim: </strong>The aim of this study was to assess and compare the influence of the presence of full-coverage crown on the obturation material removal efficiency of different retreatment file systems.</p><p><strong>Materials and methods: </strong>Sixty extracted, human, maxillary premolars were accessed, instrumented, and obturated with gutta-percha and AH Plus sealer. The samples were scanned with cone-beam computed tomography and volume of obturating material was measured using ITK-SNAP volumetric software. Samples were divided (<i>n</i> = 30) into with and without all metal full-coverage crown groups. Each group was then subdivided into three subgroups (<i>n</i> = 10) based on retreatment file used (ProTaper URS, HyFlex Remover, and NeoEndo). The volume of remnants of obturating material was remeasured by image segmentation using ITK-SNAP volumetric software. One-way analysis of variance, followed by the Tukey's post hoc test, was used for data analysis, with a significance level of 5%.</p><p><strong>Results: </strong>A significant difference in percentage remnant of obturating material after retreatment file was observed on overall comparison between with (29.84%) and without crown (21.79%) groups (<i>P</i> < 0.05). Each file system removed significantly more obturating material in without crown samples than with crown samples (<i>P</i> < 0.05). The percentage remnant of obturating material after retreatment file use was as follows: ProTaper URS > NeoEndo retreatment file > HyFlex Remover. All file systems showed significantly different (<i>P</i> < 0.05) efficiency in removal of obturating material.</p><p><strong>Conclusions: </strong>None of the retreatment file systems were able to remove obturating material completely. The presence of full-coverage crown negatively impacted the efficiency of retreatment files for the removal of obturating material. HyFlex Remover performed better than NeoEndo which was better than ProTaper URS.</p>","PeriodicalId":38892,"journal":{"name":"Journal of Conservative Dentistry","volume":"26 4","pages":"472-477"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10497092/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10316207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibitory effect of nano Stolephorus insularis and calcium hydroxide on glucosyltransferase (GTF) activity of Lactobacillus aciophilus","authors":"Tamara Yuanita, D. E. Juniarti, Tarsardo Marbun","doi":"10.20473/cdj.v13i1.2023.29-32","DOIUrl":"https://doi.org/10.20473/cdj.v13i1.2023.29-32","url":null,"abstract":"Background: Dental caries is the most common multifactorial infectious disease worldwide which referes to the process of destruction tooth hard tissue caused by bacterial by-products in the form of acids through carbohydrate fermentation. Lactobacillus acidophilus is one of the main cariogenic bacteria that causes caries by forming biofilms with the help ofglucosyltransferase (GTF) enzymes. Calcium hydroxide has limited antibacterial effects. Nano brown anchovy contains fluor as the active compound which has the potential as an antibacterial alternative agent. Purpose: To explain the inhibition of the combination of nano Stolephorus insularis and calcium hydroxide on the activity of the glucosyltransferase enzyme of Lactobacillus acidophilus bacteria. Methods: This study used a combination of nano Stolephorus insularis 3.125% with calcium hydroxide as a treatment and aquades as a control. The GTF enzyme was obtained from the supernatant centrifuged by Lactobacillus acidophilus in BHIB. The activity of the GTF enzyme was considered by calculating the fructose levels using High Performance Liquid Chromatography (HPLC) in a certain formula.Results: Decreased levels of fructose was obtained in the treatment group. From the results of Mann- Whitney data analysis, there were significant difference in the study groups. Conclusion: the combination of nano Stolephorus insularis with calcium hydroxide is effective to inhibit the glucosyltransferase enzyme activity of Lactobacillus acidophilus bacteria.","PeriodicalId":38892,"journal":{"name":"Journal of Conservative Dentistry","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89238081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect addition of epigallocatechin-3-gallate (EGCG) in nano hydroxyapatite on surface porosity as a candidate pulp capping materia","authors":"Kun Ismiyatin, N. Zubaidah, Indira Moza Azzaria","doi":"10.20473/cdj.v13i1.2023.24-28","DOIUrl":"https://doi.org/10.20473/cdj.v13i1.2023.24-28","url":null,"abstract":"Background: Deep caries, cavity preparation and use of burs or other dental instruments often result in pulpal perforation. In the case of an exposed pulp, regenerative pulp tissue treatment aims to regenerate normal tissue and maintain pulp vitality by inducing pulp regeneration using the right material, so that a good percentage and size of material porosity is needed to help cell regeneration by supporting cell proliferation and attachment, stimulating remineralization and differentiation of odontoblast-like cells. Nano-HA has the ability to produce dentine bridges continuously, has porous properties that allow cell growth, improves mechanical properties, but is not anti-inflammatory so that EGCG is added which has the advantage of being an antioxidant, anti-inflammatory which can optimize pulp tissue regeneration and also acts as an antimicrobial by reduce the growth of bacteria in the oral cavity and can trigger the proliferation and differentiation of human dental pulp cells. Purpose: This study aims to prove the difference in surface porosity of nano hydroxyapatite added with EGCG compared to nano hydroxyapatite and aquadest. Methods: This study used a laboratory experimental study with a posttest-only control group design. The research sample consisted of 32 samples which were divided into 2 groups, the treatment group (nano HA - EGCG) and the control group (nano HA - aquadest). Each research group was subjected to freeze drying and SEM tests. Results: There was a significant difference in the percentage of surface porosity between the nano hydroxyapatite added with EGCG compared to nano hydroxyapatite and aquadest.on the results of the Independent T-test (p<0.05). Conclusion: The addition of EGCG to nano hydroxyapatite has a higher porosity percentage compared to nano hydroxyapatite with aquadest.","PeriodicalId":38892,"journal":{"name":"Journal of Conservative Dentistry","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80613488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicity test of bioceramic biphasic calcium phospate (BCP) Sr-Ag doping as bone graft in BHK-21 fibroblast cells","authors":"D. Wahjuningrum, S. Setyabudi, Ria Chusnita","doi":"10.20473/cdj.v13i1.2023.33-36","DOIUrl":"https://doi.org/10.20473/cdj.v13i1.2023.33-36","url":null,"abstract":"Background: Bone graft is a substitute material that is used to assist reconstruction, stabilize the structure and bonds in bone, stimulate the osteogenesis process and as a healing bone defect. One type of bone graft that has good osteoinductive and bicompatibility is alloplast which is a synthetic calcium phosphate compound. The most frequently used Calcium Phosphate groups are Hydroxyapatite (HA), β-Tricalcium Phosphate (β-TCP), and Biphasic Calcium Phosphate (BCP). In this study the material used was BCP doping Sr2+ and Ag+. Strontium ions (Sr2+) can increase osteoblast activity, reduce osteoclast activity and cytokine production, improve osteointegration, and minimize fractures. Ag+ ion has the ability as an antibacterial agent. Purpose: To explain and prove the toxicity of bioceramic Biphasic Calcium Phosphate (BCP) doped Sr-Ag as bone graft on BHK-21 fibroblast cells. Methods: This type of research is a laboratory experiment with a post-test only control group design. Treatment with Biphasic Calcium Phosphate (BCP) doped Sr-Ag with concentrations of 200 ppm, 180 ppm, 160 ppm, 140 ppm, 120 ppm, 80 ppm, 40 ppm, 20 ppm, 10 ppm, 5 pmm, 2.5 ppm, 1.25 ppm, 0.625 ppm in BHK-21 fibroblast cell culture. Results: The percentage of fibroblast cell life at concentrations of 200 ppm, 180 ppm, 160 ppm, 140 ppm, 120 ppm, 80 ppm, 40 ppm, 20 ppm, 10 ppm, 5 pmm, 2.5 ppm, 1.25 ppm, 0.625 ppm, respectively, the percentage of live cells was 38% 44%, 46%, 50%, 52%, 65%, 69%, 71%, 72%, 75%, 77%, 81%, and 87%. The parameter used in this toxicity test is CD50. Conclusion: The results of the toxicity test of bioceramic Biphasic Calcium Phosphate (BCP) doped Sr-Ag as a bone graft showed a toxic and non-toxic effect on BHK-21 fibroblast cells at certain concentrations.","PeriodicalId":38892,"journal":{"name":"Journal of Conservative Dentistry","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81572708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}