Journal of Conservative Dentistry最新文献

筛选
英文 中文
Color changes of nanofiller composite resin after glycerin application immersed in turmeric extract 甘油浸在姜黄提取物中后纳米填料复合树脂的颜色变化
Journal of Conservative Dentistry Pub Date : 2023-06-30 DOI: 10.20473/cdj.v13i1.2023.37-41
Sukaton, Galih Sampoerno, Widyajeng Ayu Laksmi, Daradhasih Bestari Santiaji
{"title":"Color changes of nanofiller composite resin after glycerin application immersed in turmeric extract","authors":"Sukaton, Galih Sampoerno, Widyajeng Ayu Laksmi, Daradhasih Bestari Santiaji","doi":"10.20473/cdj.v13i1.2023.37-41","DOIUrl":"https://doi.org/10.20473/cdj.v13i1.2023.37-41","url":null,"abstract":"Background: Nanofiller composite resin has good mechanical strength also used for restoration that require aesthetics. However, resin composites can experience discoloration resulting in extrinsic or intrinsic factors. To produce perfect polymerization, glycerin can be used as an air-inhibition coating to prevent the formation of Oxygen-Inhibited Layer resulting in a more stable over discoloration. Purpose: To knowing the color change due to the application of glycerin on nanofiller composite resin immersed in turmeric extract solution. Method: It is a laboratory experimental study with a Pre-Test-Post-Test Control Group Design using 28 samples of 3M ESPE Filtek 350XT nanofiller composite resin divided into 4 groups, namely the group with glycerin application and without glycerin application which were soaked in turmeric extract with and saline for 4 days. The color changes were measured using a CHNSpec CS-10 colorimeter before and after immersion. Results: Nanofiller composite resin with turmeric extract immersion without glycerin application had an average discoloration of 90.97 + 23.26 while with glycerin application it had a lower average of 84.37 + 9.24. On the results of the independent sample t-test with a significant level of 5%, it has a value of p = 0.506 > 0.05. Conclusion: Composite resin with glycerin application experienced less color change than composite resin without glycerin application in turmeric extract immersion, but statistically not significant.","PeriodicalId":38892,"journal":{"name":"Journal of Conservative Dentistry","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81511046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of nonthermal atmospheric plasma on bond strength of composite resin using total-etch and self-etch adhesive systems. 使用全蚀刻和自蚀刻粘合剂系统的非热大气等离子体对复合树脂粘合强度的影响。
Journal of Conservative Dentistry Pub Date : 2023-05-01 Epub Date: 2023-05-16 DOI: 10.4103/jcd.jcd_33_23
Nagesh Bolla, Aameena Banu Mayana, Praveen Kumar Gali, Sayesh Vemuri, Roopadevi Garlapati, Shaik Afreen Kamal
{"title":"Effect of nonthermal atmospheric plasma on bond strength of composite resin using total-etch and self-etch adhesive systems.","authors":"Nagesh Bolla,&nbsp;Aameena Banu Mayana,&nbsp;Praveen Kumar Gali,&nbsp;Sayesh Vemuri,&nbsp;Roopadevi Garlapati,&nbsp;Shaik Afreen Kamal","doi":"10.4103/jcd.jcd_33_23","DOIUrl":"10.4103/jcd.jcd_33_23","url":null,"abstract":"<p><strong>Aim: </strong>The aim of this study was to assess the impact of nonthermal atmospheric plasma (NTAP) on the bond strength of composite resin following plasma application at different steps of dentin bonding in total-etch and self-etch adhesive systems.</p><p><strong>Materials and methods: </strong>Ninety extracted third molars were taken, and the occlusal surfaces were removed until the exposure of the dentin. Samples were distributed into two main groups: Group T: total-etch adhesive system and Group S: self-etch adhesive system. Groups are further subdivided (<i>n</i> = 10) based on plasma application at different steps of dentin bonding. T1: surface etching with 37% phosphoric acid and bonding agent application. T2: plasma application and bonding agent application. T3: plasma application, etching, and bonding agent application. T4: etching, plasma application, and bonding agent application. T5: etching, plasma application, bonding agent application, and again plasma application. S1: self-etch bonding agent application. S2: plasma application and bonding agent application. S3: Bonding agent application and plasma application. S4: plasma application, bonding agent application, and again plasma application. For all the samples, composite resin buildup was done, and shear bond strength (SBS) was measured. The contact angle was measured at different steps of dental adhesive systems.</p><p><strong>Statistical analysis: </strong>Two-way analysis of variance and the post hoc Tukey's test were used for analysis, regarding <i>P</i> < 0.05 as statistically significant.</p><p><strong>Results: </strong>Among all groups of total-etch and self-etch adhesives, Group T4 (48.81 Mpa) and Group S2 (36.59 Mpa), respectively, have demonstrated significantly greater bond strength values than the corresponding control groups.</p><p><strong>Conclusion: </strong>NTAP enhanced the composite resin's SBS when plasma treatment was done before bonding agent application and significantly reduced the contact angles of the distilled water.</p>","PeriodicalId":38892,"journal":{"name":"Journal of Conservative Dentistry","volume":"26 3","pages":"292-298"},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10309117/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9743971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
A confocal laser scanning microscopic evaluation of nonthermal atmospheric plasma on the dentinal tubule penetration of bioceramic and epoxy resin-based root canal sealers. 非热大气等离子体对生物陶瓷和环氧树脂基根管封闭剂穿透牙本质小管的共焦激光扫描显微镜评价。
Journal of Conservative Dentistry Pub Date : 2023-05-01 Epub Date: 2023-05-16 DOI: 10.4103/jcd.jcd_22_23
Roopadevi Garlapati, Praveen Kumar Gali, Nagesh Bolla, Bandlapally Sreenivasa Guptha Anila, Sayesh Vemuri, Bandaru Pydiah Naidu
{"title":"A confocal laser scanning microscopic evaluation of nonthermal atmospheric plasma on the dentinal tubule penetration of bioceramic and epoxy resin-based root canal sealers.","authors":"Roopadevi Garlapati,&nbsp;Praveen Kumar Gali,&nbsp;Nagesh Bolla,&nbsp;Bandlapally Sreenivasa Guptha Anila,&nbsp;Sayesh Vemuri,&nbsp;Bandaru Pydiah Naidu","doi":"10.4103/jcd.jcd_22_23","DOIUrl":"10.4103/jcd.jcd_22_23","url":null,"abstract":"<p><strong>Aim: </strong>Using confocal laser scanning microscopy (CLSM), the current study assessed the impact of nonthermal atmospheric plasma (NTAP) on the dentinal tubule penetration of bioceramic and epoxy resin-based root canal sealers (CLSM).</p><p><strong>Materials and methods: </strong>Forty human mandibular premolar teeth with a single root that had just undergone extraction were chosen and biomechanical preparation of root canals was done with ProTaper Gold rotary Nickel-titanium instruments. Samples were divided into four groups (<i>n</i> = 10). Group 1: Bioceramic sealer (BioRoot RCS); Group 2: Epoxy resin-based sealer (AH Plus) without application of NTAP; Group 3: Bioceramic sealer (BioRoot RCS); and Group 4: Epoxy resin-based sealer (AH Plus) with the application of NTAP for 30 s. In Groups 3 and 4, all of the samples underwent obturation with the appropriate sealers following NTAP application. For determination of the sealer's dentinal tubule penetration values, slices with a thickness of 2 mm were taken from the middle third of sample's root and examined using CLSM. The acquired data were statistically analyzed with one-way analysis of variance and the <i>Post hoc</i> Tukey's test. The cutoff for statistical significance was <i>P</i> < 0.05.</p><p><strong>Results: </strong>In comparison to other groups, the maximum sealer penetration values into dentinal tubules were significantly higher in Group 3 for Bioceramic sealer with NTAP application and Group 4 for Epoxy resin-based sealer with NTAP application.</p><p><strong>Conclusion: </strong>NTAP application increased the dentinal tubule penetration of bioceramic and epoxy resin-based sealers in comparison to groups without NTAP application.</p>","PeriodicalId":38892,"journal":{"name":"Journal of Conservative Dentistry","volume":"26 3","pages":"265-270"},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10309127/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9746311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An in vitro comparison of alterations in surface topographies of three different rotary files after root canal preparation with different irrigating solutions: Atomic force microscopic study. 三种不同旋转锉在不同冲洗溶液根管预备后表面形貌变化的体外比较:原子力显微镜研究。
Journal of Conservative Dentistry Pub Date : 2023-05-01 Epub Date: 2023-05-16 DOI: 10.4103/jcd.jcd_72_23
Prem Sai Parepalli, T B V G Raju, P Krishna Prasad, Gowtam Dev Dondapati, Venkata Srija Kintada, Alekhya Mediboyina
{"title":"An <i>in vitro</i> comparison of alterations in surface topographies of three different rotary files after root canal preparation with different irrigating solutions: Atomic force microscopic study.","authors":"Prem Sai Parepalli,&nbsp;T B V G Raju,&nbsp;P Krishna Prasad,&nbsp;Gowtam Dev Dondapati,&nbsp;Venkata Srija Kintada,&nbsp;Alekhya Mediboyina","doi":"10.4103/jcd.jcd_72_23","DOIUrl":"10.4103/jcd.jcd_72_23","url":null,"abstract":"<p><strong>Objectives: </strong>The objective of the study was to evaluate and compare the surface topographies of WaveOne Gold (WOG), FlexiCON X1, and EdgeOne Fire (EOF) reciprocating files before and after root canal instrumentation using different irrigating solutions.</p><p><strong>Materials and methods: </strong>Forty-eight extracted mandibular molars were selected and randomly assigned into three groups (<i>n</i> = 16) based on the file system used and each group was divided into two subgroups based on the irrigants used during root canal treatment. (Group-1: WOG; Group-2: FlexiCON X1; Group-3: EOF; Subgroup-A: 3% sodium hypochlorite [NaOCl] +17% ethylenediaminetetraacetic acid [EDTA]; Subgroup-B: Citra wash as irrigating solutions). The surface topography of the files was analyzed using atomic force microscope before and after instrumentation. Average roughness and root mean square roughness were calculated. Independent and paired <i>t</i>-tests and one-way analysis of variance with Tukey's post hoc were used for statistical analysis.</p><p><strong>Results: </strong>Atomic force microscopy results showed an increase in surface roughness after instrumentation, EOF showing the highest roughness. Citra wash showed more roughness compared to NaOCl and EDTA together. However, the surface roughness between the experimental groups WOG and EOF are statistically insignificant and this insignificance was also seen among subgroups (P>0.05).</p><p><strong>Conclusion: </strong>Instrumentation with various irrigating solutions affected the surface topography of EOF, WOG, and FlexiCON X1 reciprocating files.</p>","PeriodicalId":38892,"journal":{"name":"Journal of Conservative Dentistry","volume":"26 3","pages":"299-304"},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10309126/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9749597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A rare case of persistent postendodontic symptomatic maxillary central incisor with aberrant canal configuration confirmed by cone-beam computer tomography and its nonsurgical management by retreatment. 一例罕见的持续性根管后症状上颌中切牙,经锥形束计算机断层扫描证实其管结构异常,并通过再治疗进行非手术治疗。
Journal of Conservative Dentistry Pub Date : 2023-05-01 Epub Date: 2023-05-16 DOI: 10.4103/jcd.jcd_64_23
Anirban Bhattacharyya, Gayatri Majumder, Asim Bikash Maity, Soham Datta
{"title":"A rare case of persistent postendodontic symptomatic maxillary central incisor with aberrant canal configuration confirmed by cone-beam computer tomography and its nonsurgical management by retreatment.","authors":"Anirban Bhattacharyya,&nbsp;Gayatri Majumder,&nbsp;Asim Bikash Maity,&nbsp;Soham Datta","doi":"10.4103/jcd.jcd_64_23","DOIUrl":"10.4103/jcd.jcd_64_23","url":null,"abstract":"<p><p>Maxillary central incisor is considered the tooth with least anatomical variations. In literature, the prevalence of single root and single canal in maxillary central incisor has been reported as 100%. Only a handful of case reports suggesting more than one root or one canal are available and are mostly associated with developmental anomalies such as gemination and fusion. This article describes a rare case report of retreatment of a maxillary central incisor with two roots with normal clinical crown which was confirmed by cone-beam computer tomography (CBCT). A 50-year-old Indian male patient presented with pain and discomfort on a root canal-treated anterior tooth. Pulp sensibility testing of left maxillary central incisor was negative. Intraoral periapical digital radiograph revealed an obturated canal with suspected outline of a second root which got confirmed with cone shift technique. The tooth was treated under dental operating microscope during which two canals were located and retreatment was completed. Postobturation, CBCT was performed to study the root and canal morphology. Clinically and radiographically, all the follow-up examinations revealed an asymptomatic tooth without any active periapical lesion. The present case report emphasizes the fact that clinicians should approach each case with an open mind having a thorough knowledge of the normal tooth anatomy and should suspect variations in every case to ensure successful endodontic outcome.</p>","PeriodicalId":38892,"journal":{"name":"Journal of Conservative Dentistry","volume":"26 3","pages":"359-363"},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10309136/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9749595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparative evaluation of cleaning efficiency of single file NiTi rotary system during root canal treatment procedure - A scanning electron microscope study. 单锉NiTi旋转系统在根管治疗过程中清洁效率的比较评估——扫描电子显微镜研究。
Journal of Conservative Dentistry Pub Date : 2023-05-01 Epub Date: 2023-05-16 DOI: 10.4103/jcd.jcd_52_23
Ruchi Vashisht, Umesh Kumar, Swaty Jhamb, Ruchi Singla
{"title":"Comparative evaluation of cleaning efficiency of single file NiTi rotary system during root canal treatment procedure - A scanning electron microscope study.","authors":"Ruchi Vashisht,&nbsp;Umesh Kumar,&nbsp;Swaty Jhamb,&nbsp;Ruchi Singla","doi":"10.4103/jcd.jcd_52_23","DOIUrl":"10.4103/jcd.jcd_52_23","url":null,"abstract":"<p><strong>Background: </strong>Optimal biomechanical preparation, thorough irrigation, proper disinfection, and finally, well-sealed obturation is a must to attain definitive success in root canal procedure. Proper preparation of the root canal is all the more imperative to establish a hermetic apical seal with adequately placed filling materials. The present study was conducted for comparing the Cleaning Efficiency of F360 system and the WaveOne Gold (WOG) system of rotary NiTi instrumentation for a root canal.</p><p><strong>Materials and methods: </strong>One hundred freshly extracted noncarious mandibular canines were obtained. Access cavity of standard size was made followed by the establishment of working length. Afterward, random division of all the specimens was done into two study groups: group A: the group in which F360 system was used for instrumentation and group B: the group in which the WOG system was used for instrumentation. Irrigations of all the specimens of both the study groups were done followed by root canal shaping by the instrument system of their respective study groups. Scanning electron microscope (SEM) was used for assessment after specimens were cut buccolingually. Debris score and residual smear layer score were used for assessment.</p><p><strong>Results: </strong>Among group A specimens, the mean smear layer score at the coronal third, middle third, and apical third was 1.76, 2.39, and 2.65, respectively. Among group B specimens, the mean smear layer score at the coronal third, middle third, and apical third was 1.34, 1.59, and 1.92, respectively. While analyzing statistically, it was seen that the mean smear layer score was significantly higher among specimens of group A in comparison to group B. While analyzing statistically, it was seen that the mean debris score was significantly higher among specimens of group A in comparison to group B.</p><p><strong>Conclusion: </strong>The cleaning effectiveness of WOG instruments was significantly enhanced in comparison to F360 equipment.</p>","PeriodicalId":38892,"journal":{"name":"Journal of Conservative Dentistry","volume":"26 3","pages":"316-320"},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10309122/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9749599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
The revolutionary evolution in carious lesion management. 龋齿病变管理的革命性发展。
Journal of Conservative Dentistry Pub Date : 2023-05-01 Epub Date: 2023-05-16 DOI: 10.4103/jcd.jcd_54_23
Nebu Philip, Bharat Suneja
{"title":"The revolutionary evolution in carious lesion management.","authors":"Nebu Philip, Bharat Suneja","doi":"10.4103/jcd.jcd_54_23","DOIUrl":"10.4103/jcd.jcd_54_23","url":null,"abstract":"<p><p>Contemporary paradigms of dental caries management focus on the biological approaches to treating the disease and its principal symptom, the carious lesion. This narrative review traces the evolution of carious lesion management from the operative and invasive approaches of G. V. Black's era to the current period of minimally invasive biological approaches. The paper explains the rationale for adopting biological approaches to dental caries management and lists the five core principles of this management approach. The paper also details the aims, features, and the most recent evidence base for the different biological carious lesion management approaches. Based on current practice guidelines, collated clinical pathways for lesion management are also presented in the paper to aid clinicians in their decision-making. It is hoped that the biological rationale and evidence summarized in this paper will bolster the shift to modern biological carious lesion management approaches among dental practitioners.</p>","PeriodicalId":38892,"journal":{"name":"Journal of Conservative Dentistry","volume":"26 3","pages":"249-257"},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10309123/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9749596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Conservative esthetic management of brown enamel fluorosis using combination therapy: A clinical report. 应用联合疗法保守美学治疗棕色釉质氟中毒:一份临床报告。
Journal of Conservative Dentistry Pub Date : 2023-05-01 Epub Date: 2022-12-08 DOI: 10.4103/jcd.jcd_632_20
Lim Songping Nicholas, Quek Eng Yew Christopher, Lee Kong Fei Frank
{"title":"Conservative esthetic management of brown enamel fluorosis using combination therapy: A clinical report.","authors":"Lim Songping Nicholas,&nbsp;Quek Eng Yew Christopher,&nbsp;Lee Kong Fei Frank","doi":"10.4103/jcd.jcd_632_20","DOIUrl":"10.4103/jcd.jcd_632_20","url":null,"abstract":"<p><p>Dental fluorosis is caused by ingestion of excessive fluoride during tooth development resulting in hypomineralization of the enamel and can present as white or brown intrinsic lesions. This case report describes a combination of minimally invasive techniques: microabrasion, bleaching, and resin infiltration to address brown enamel fluorosis present on the maxillary anterior teeth of a young patient. Before resin infiltration, air microabrasion was targeted at subsurface lesions located on the maxillary central and lateral incisors, and chairside bleaching was done with 37% hydrogen peroxide (Opalescence). After which, hypoplastic lesions on the buccal surfaces were etched before being treated with two sessions of resin infiltration (ICON and DMG). Following treatment, satisfactory esthetic outcomes were achieved. Accurate diagnosis, depth of lesions, and understanding the effectiveness and limitations of each technique are essential to select the appropriate type of treatment to obtain the best esthetic outcome. In conclusion, the conservative management of varying severity of dental fluorosis may require the appropriate use of combination treatment modalities, such as microabrasion, bleaching and resin infiltration, when clinically indicated, to address the treatment needs and achieve a desired outcome.</p>","PeriodicalId":38892,"journal":{"name":"Journal of Conservative Dentistry","volume":"26 3","pages":"349-354"},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10309132/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9743974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Intracellular stress caused by composite resins: An in vitro study using a bioluminescent antioxidant-responsive element reporter assay. 复合树脂引起的细胞内应激:一项使用生物发光抗氧化反应元件报告基因测定的体外研究。
Journal of Conservative Dentistry Pub Date : 2023-05-01 Epub Date: 2023-05-16 DOI: 10.4103/jcd.jcd_30_23
Mari Masuda, Miki Hori, Junko Inukai, Takahiro Suzuki, Satoshi Imazato, Tatsushi Kawai
{"title":"Intracellular stress caused by composite resins: An <i>in vitro</i> study using a bioluminescent antioxidant-responsive element reporter assay.","authors":"Mari Masuda,&nbsp;Miki Hori,&nbsp;Junko Inukai,&nbsp;Takahiro Suzuki,&nbsp;Satoshi Imazato,&nbsp;Tatsushi Kawai","doi":"10.4103/jcd.jcd_30_23","DOIUrl":"10.4103/jcd.jcd_30_23","url":null,"abstract":"<p><strong>Context: </strong>Elucidating the effects of leachates from composite resins (CRs) on cells by examining the transcription level of detoxification genes and the antioxidant-responsive element (ARE), would be helpful in clinical practice.</p><p><strong>Aims: </strong>The aim of the study is to investigate the cytotoxicity of commercially available CRs, we used a reporter assay system to evaluate intracellular stress based on ARE-mediated transcription.</p><p><strong>Setting and design: </strong>The study design was an <i>in vitro</i> study.</p><p><strong>Materials and methods: </strong>Seven kinds of CRs were each placed in four-well plates to which culture medium was added and then light-cured. The prepared samples were used either immediately (sample A) or after incubation at 37°C for 24 h (sample B) in the subsequent ARE-luciferase reporter assay, in which HepG2 cells stably expressing an ARE-regulated luciferase reporter gene (HepG2-AD13 cells) were cultured for 6 h in culture media with the CR eluate (samples A or B) or without (control) (<i>n</i> = 4). In the cell viability assay, cell viability in various solutions with the same incubation time was confirmed by MTT assay (<i>n</i> = 4). Statistical analysis was performed using the paired <i>t</i>-test and one-way analysis of variance.</p><p><strong>Results: </strong>All CR solutions showed an increase in ARE activation rate; a CR with spherical nanofillers showed the highest ARE activation rate of 108.5-fold in sample A. Cell viability was not significantly reduced for any of the CRs in sample A. However, the CR-containing bisphenol A-glycidyl methacrylate (Bis-GMA) caused a significant decrease in cell viability in sample B.</p><p><strong>Conclusions: </strong>The intracellular stress in the viable cells differed among the CRs, depending on the type of monomer used. In particular, Bis-GMA-containing hydroxyl groups showed high cytotoxicity.</p>","PeriodicalId":38892,"journal":{"name":"Journal of Conservative Dentistry","volume":"26 3","pages":"275-280"},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10309129/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9743972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Influence of the restoration after pulpotomy on the strength of electrical stimulus reaching the pulp space: An in vitro investigation. 牙髓切断术后修复对到达牙髓间隙的电刺激强度的影响:体外研究。
Journal of Conservative Dentistry Pub Date : 2023-05-01 Epub Date: 2023-05-16 DOI: 10.4103/jcd.jcd_67_23
Aakanksha Chopra, Sidhartha Sharma, Vijay Kumar, Amrita Chawla, Suman Jain, Ajay Logani
{"title":"Influence of the restoration after pulpotomy on the strength of electrical stimulus reaching the pulp space: An <i>in vitro</i> investigation.","authors":"Aakanksha Chopra,&nbsp;Sidhartha Sharma,&nbsp;Vijay Kumar,&nbsp;Amrita Chawla,&nbsp;Suman Jain,&nbsp;Ajay Logani","doi":"10.4103/jcd.jcd_67_23","DOIUrl":"10.4103/jcd.jcd_67_23","url":null,"abstract":"<p><strong>Introduction: </strong>The study evaluated the influence of coronal restoration after pulpotomy on the strength of electrical stimulus reaching the radicular pulp using an electric pulp test (EPT).</p><p><strong>Materials and methods: </strong>The pulp tissue from ten freshly extracted mandibular premolar teeth was removed and replaced with an electroconductive gel. The cathode probe of Powerlab was inserted into the pulp space and the anode probe was attached to the EPT handpiece. The EPT probe coated with electro-conducting material was positioned in the middle third of the buccal crown surface. The EPT stimulus reaching the pulp space of an intact tooth at 40 numerical readings was recorded. The tooth was removed from model and endodontic access was made. The 2-mm thick mineral trioxide aggregate was placed at the cementoenamel junction followed by composite resin restoration. The experimental setup was re-established and postpulpotomy EPT stimulus data were recorded. The data collected were compared using the Wilcoxon signed-rank test.</p><p><strong>Results: </strong>There was a statistically significant difference (<i>P</i> = 0.038) between observed between the strength of EPT stimulus reaching the pulp space in prepulpotomy (mean 91.18 ± 101.02 V and median 25.79 V) and postpulpotomy (mean 58.49 ± 77.13 V and median 13.75 V) tooth samples.</p><p><strong>Conclusion: </strong>The placement of the restoration and pulp capping agent after pulpotomy dampens the strength of EPT stimulus reaching the pulp canal space.</p>","PeriodicalId":38892,"journal":{"name":"Journal of Conservative Dentistry","volume":"26 3","pages":"338-343"},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10309130/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9746312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信