Korean Journal of Veterinary Research最新文献_第5页

Rabies neutralizing antibody titers in Korean dogs and cats intended for overseas travel 用于海外旅行的韩国猫狗狂犬病中和抗体滴度

Korean Journal of Veterinary Research Pub Date : 2023-06-30 DOI: 10.14405/kjvr.20220043

Dong-Kun Yang, Yu-Ri Park, Ha-Hyun Kim, Eun-ju Kim, H. Lee, Young-Ju Cheong, B. Hyun

引用次数: 0

Serological survey for Getah virus in domestic pigs of South Korea 韩国家猪Getah病毒血清学调查

Korean Journal of Veterinary Research Pub Date : 2023-03-31 DOI: 10.14405/kjvr.20220034

Yu-Ri Park, Eun-ju Kim, H. Lee, B. Hyun, Dong-Kun Yang

引用次数: 0

Retraction: Isolation, characterization and neutralizing activity of porcine epidemic diarrhea viruses from Vietnam 撤回:越南猪流行性腹泻病毒的分离、鉴定和中和活性

Korean Journal of Veterinary Research Pub Date : 2023-03-31 DOI: 10.14405/kjvr.2021.61.e23.r1

Van Tan Do, Quang Lam Truong, Hoai Thu Dao, Thi Lan Nguyen, Jini Kim, Tae-Wook Hahn

引用次数: 0

Differential cytotoxic effects of fenbendazole on mouse lymphoma EL-4 cells and spleen cells 芬苯达唑对小鼠淋巴瘤EL-4细胞和脾细胞的差异细胞毒作用

Korean Journal of Veterinary Research Pub Date : 2023-03-31 DOI: 10.14405/kjvr.20220040

Hae-Jung Jung, You-Jeong Lee, H. Joo

{"title":"Differential cytotoxic effects of fenbendazole on mouse lymphoma EL-4 cells and spleen cells","authors":"Hae-Jung Jung, You-Jeong Lee, H. Joo","doi":"10.14405/kjvr.20220040","DOIUrl":"https://doi.org/10.14405/kjvr.20220040","url":null,"abstract":"Fenbendazole (FBZ) is a benzimidazole anthelmintic widely used to treat parasitic infections. The anticancer effect of FBZ has been recently highlighted leading to its consideration as a potential anticancer agent. Although previous studies have demonstrated the effect of FBZ on cancer cells, there is a paucity of studies on the effect of FBZ on lymphoma cells and normal immune cells. Herein, we investigated the effects of FBZ on a mouse lymphoma cell line, EL-4 cells, and spleen cells, using vincristine as a positive control. The cellular metabolic activity of EL-4 cells was decreased by FBZ, but that of the spleen cells was not decreased. Moreover, FBZ reduced the mitochondrial membrane potential and induced reactive oxygen species production in EL-4 cells, but not in spleen cells. FBZ induced G2/M phase arrest and increased the sub G0/G1 phase ratio, indicating apoptosis. Furthermore, compared to the control cells, the reactivity of spleen cells pretreated with FBZ to lipopolysaccharide was maintained. In summary, FBZ is cytotoxic to EL-4 cells, but not to spleen cells. This study provides experimental evidence that FBZ exerts an anticancer effect, and less cytotoxic effects and functional damage to normal spleen cells.","PeriodicalId":38891,"journal":{"name":"Korean Journal of Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44466631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunogenicity of a new inactivated vaccine against feline panleukopenia virus, calicivirus, and herpesvirus-1 for cats 猫全白细胞减少症病毒、杯状病毒和疱疹病毒-1灭活疫苗的免疫原性

Korean Journal of Veterinary Research Pub Date : 2023-03-31 DOI: 10.14405/kjvr.20220042

Dong-Kun Yang, Yu-Ri Park, Eun-ju Kim, H. Lee, Subin Oh, B. Hyun

{"title":"Immunogenicity of a new inactivated vaccine against feline panleukopenia virus, calicivirus, and herpesvirus-1 for cats","authors":"Dong-Kun Yang, Yu-Ri Park, Eun-ju Kim, H. Lee, Subin Oh, B. Hyun","doi":"10.14405/kjvr.20220042","DOIUrl":"https://doi.org/10.14405/kjvr.20220042","url":null,"abstract":"Feline panleukopenia virus (FPV), feline calicivirus (FCV), and feline herpesvirus type-1 (FHV-1) are major infectious pathogens in cats. We evaluated the immunogenicity of a new vaccine containing inactivated FPV, two FCVs, and FHV-1 in animals. An FPV, two FCVs, and an FHV-1 isolate were continuously passaged 70, 50, 80, and 100 times in CRFK cells. FP70, FC50, FC80, and FH100 were propagated and used as vaccine antigens. Two inactivated feline virus vaccines, Rehydragel-adjuvanted vaccine (FRAV) and Cabopol-adjuvanted vaccine (FCAV) were prepared and inoculated into mice and guinea pigs. Humoral immune responses were measured using hemagglutination inhibition (HI) for FPV and virus-neutralizing antibody (VNA) for two FCVs and FHV-1 tests. Serial passages in CRFK cells resulted in increase in titers of FPV and two FCVs but not FHV-1 The FCAV induced higher mean HI and VNA titers than the FRAV in guinea pigs; therefore, the FCAV was selected. Cats inoculated with FCAV developed a mean HI titer of 259.9 against FPV, and VNA titers of 64, 256, and 3.2 against FCV17D03, FCV17D283, and FHV191071, respectively. Therefore, cats inoculated with the FCAV showed a considerable immune response after receiving a booster vaccination.","PeriodicalId":38891,"journal":{"name":"Korean Journal of Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45361983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence of infectious reproductive diseases in sows from Tolima-Colombia 哥伦比亚托利马母猪传染性生殖疾病的患病率

Korean Journal of Veterinary Research Pub Date : 2023-03-31 DOI: 10.14405/kjvr.20220039

Nicolas Carrillo-Godoy, Valentina Rueda-García, H. F. Uribe-García, I. Rondón-Barragán

引用次数: 0

Hemorrhagic disease caused by bovine viral diarrhea virus-2a in Korean Indigenous Cattle: case reports 韩国本土牛病毒性腹泻病毒2a引起的出血性疾病:病例报告

Korean Journal of Veterinary Research Pub Date : 2023-03-31 DOI: 10.14405/kjvr.20230005

Hyung-Chul Cho, Byoung-Soo Kim, Dong-Hun Jang, Kyunghyun Lee, Kyoung-Seong Choi

{"title":"Hemorrhagic disease caused by bovine viral diarrhea virus-2a in Korean Indigenous Cattle: case reports","authors":"Hyung-Chul Cho, Byoung-Soo Kim, Dong-Hun Jang, Kyunghyun Lee, Kyoung-Seong Choi","doi":"10.14405/kjvr.20230005","DOIUrl":"https://doi.org/10.14405/kjvr.20230005","url":null,"abstract":"Bovine viral diarrhea virus (BVDV), which is prevalent worldwide, is one of the most important viral pathogens and causes substantial economic loss to the livestock industry. Despite its importance, BVDV is largely unnoticed in the Republic of Korea (ROK). In this study, we report the case of a steer with BVDV that died suddenly due to severe enteritis. Two 1-year-old Korean indigenous cattle in the same herd presented severe hemorrhagic diarrhea. Case 1 had severe dehydration and died after 3 days, whereas case 2 had anorexia, depression, and severe diarrhea with mucus and blood. Only case 2 was necropsied, and BVDV2a was detected in the tissues of its alimentary tract. Gross lesions, including erosion, ulceration, and extensive hemorrhage, were observed in the digestive tract mucosa. Immunohistochemistry revealed marked positive staining for BVDV2a antigen in the large intestine. This report describes the first case of hemorrhagic disease caused by acute BVDV2a infection, which is characterized by high mortality in Korean indigenous cattle. This study will help establish vaccination and control strategies for BVDV in the ROK.","PeriodicalId":38891,"journal":{"name":"Korean Journal of Veterinary Research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41836450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developmental competence of chimeric porcine embryos through the aggregation of parthenogenetic embryos and somatic cell nuclear transfer embryos 通过孤雌胚胎和体细胞核移植胚胎的聚集研究嵌合猪胚胎的发育能力

Korean Journal of Veterinary Research Pub Date : 2023-03-31 DOI: 10.14405/kjvr.20230003

Joohyeong Lee, L. Cai, Mirae Kim, Hyerin Choi, D. Oh, Ali Jawad, Eunsong Lee, S. Hyun

{"title":"Developmental competence of chimeric porcine embryos through the aggregation of parthenogenetic embryos and somatic cell nuclear transfer embryos","authors":"Joohyeong Lee, L. Cai, Mirae Kim, Hyerin Choi, D. Oh, Ali Jawad, Eunsong Lee, S. Hyun","doi":"10.14405/kjvr.20230003","DOIUrl":"https://doi.org/10.14405/kjvr.20230003","url":null,"abstract":"The efficiency of somatic cell nuclear transfer (NT) in pigs is low and requires enhancement. We identified the most efficient method for zona pellucida (ZP) removal and blastomere aggregation in pigs and investigated whether the aggregation of NT and parthenogenetic activation (PA) of blastomeres could reduce embryonic apoptosis and improve the quality of NT-derived embryos by investigating. Embryonic developmental competence after ZP removal using acid Tyrode's solution or protease (pronase E). The embryonic developmental potential of NT-derived blastomeres was also investigated using well-of-the-well or phytohemagglutinin-L. We analyzed apoptosis in aggregate-derived blastocysts. The aggregation rate of protease-treated embryos was lower than that of Tyrode’s solution-treated embryos (69.2% vs. 88.3%). No significant difference was observed between phytohemagglutinin-L and well-of-the-well (35.7%–38.5%). However, 2P1N showed a higher number of blastocysts compared to 3N (73.8% vs. 24.3%) and an increased blastocyst diameter compared to the control and 1P2N (274 μm vs. 230–234 μm). In blastomeres aggregated using phytohemagglutinin-L, the apoptotic cell ratio was significantly higher in 1P2N and 3N than in 3P (5.91%–6.46% vs. 2.94%, respectively). Our results indicate that aggregation of one NT embryo with two PA embryos improved the rate of blastocysts with increased blastocyst diameter.","PeriodicalId":38891,"journal":{"name":"Korean Journal of Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48399316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alleviation of γ-enolase decrease by the chlorogenic acid administration in the stroke animal model 绿原酸对脑卒中动物模型γ烯醇化酶降低的缓解作用

Korean Journal of Veterinary Research Pub Date : 2023-03-31 DOI: 10.14405/kjvr.20230008

Ju-Bin Kang, Murad-Ali Shah, Min-Seo Ko, P. Koh

{"title":"Alleviation of γ-enolase decrease by the chlorogenic acid administration in the stroke animal model","authors":"Ju-Bin Kang, Murad-Ali Shah, Min-Seo Ko, P. Koh","doi":"10.14405/kjvr.20230008","DOIUrl":"https://doi.org/10.14405/kjvr.20230008","url":null,"abstract":"Stroke is a major cause of death and long-term disability. Chlorogenic acid is a phenolic compound with a potent neuroprotective effect. γ-enolase is a phosphopyruvate hydratase found in mature neurons and plays an important role in the neuronal survival. This study investigated whether chlorogenic acid regulates the expression of γ-enolase during cerebral ischemia. Middle cerebral artery occlusion (MCAO) was performed to indcue cerebral ischemia. Adult male rats were used and chlorogenic acid (30 mg/kg) or phosphate buffered saline (PBS) was injected intraperitoneally 2 hours after MCAO surgery. Cerebral cortical tissues were collected 24 hours after MCAO surgery. Our proteomic approach identified the reduction of γ-enolase caused by MCAO damage and the mitigation of this reduction by chlorogenic acid treatment. Results of reverse transcription-polymerase chain reaction and Western blot analyses showed decrease in γ-enolase expression in PBS-treated MCAO group. However, chlorogenic acid treatment attenuated this decrease. Results of immunofluorescence staining showed the change of γ-enolase by chlorogenic acid treatment. These results demonstrated that chlorogenic acid regulates the γ-enolase expression during MCAO-induced ischemia. Therefore, we suggest that chlorogenic acid mediates the neuroprotective function by regulating the γ-enolase expression in cerebral ischemia and may be used as a therapeutic agent for brain diseases including stroke.","PeriodicalId":38891,"journal":{"name":"Korean Journal of Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46947974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serological responses and protection levels in chickens administered with Newcastle disease vaccines 新城疫疫苗对鸡的血清学反应和保护水平

Korean Journal of Veterinary Research Pub Date : 2022-12-31 DOI: 10.14405/kjvr.20220031

Geumji Seung, Ji-Yeon Kim, Hyobi Kim, Ji-Yeon Kim, Yang-Ho Jang, Y. Kim, M. Her, S. Yi, Keun-Woo Lee, Il Jang, Y. J. Lee

{"title":"Serological responses and protection levels in chickens administered with Newcastle disease vaccines","authors":"Geumji Seung, Ji-Yeon Kim, Hyobi Kim, Ji-Yeon Kim, Yang-Ho Jang, Y. Kim, M. Her, S. Yi, Keun-Woo Lee, Il Jang, Y. J. Lee","doi":"10.14405/kjvr.20220031","DOIUrl":"https://doi.org/10.14405/kjvr.20220031","url":null,"abstract":"Vaccination against Newcastle disease (ND) is the most effective means of controlling the disease, and these vaccines are commercialized only after their safety and effectiveness have been verified through tests that comply with Korean Standards of National Lot Release for Veterinary Biologics. This study investigated whether a relatively convenient and safe serological test can be used in place of the challenge test using highly virulent ND virus. Hemagglutination inhibition (HI) assay and enzyme-linked immunosorbent assay (ELISA) were considered positive of log2 2 or more and cutoff value of 200 or more, respectively, in both live and inactivated vaccines. However, when the antibody levels of the live and inactivated vaccines induced using the Ulster 2C, KBNP-C4152R2L, and K148/08 strains were compared, the antibody titers for inactivated vaccines were significantly higher than those for live vaccines in both the HI assay and ELISA. A strong positive correlation was observed between HI and ELISA antibody titers. The live vaccines corresponded to a survival rates of ≥ 80% and the inactivated vaccines corresponded to 100% survival rates. This study confirmed that standard efficacy tests can serve as serological tests, and can replace the challenge test and that the vaccine approval process can be improved.","PeriodicalId":38891,"journal":{"name":"Korean Journal of Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42399092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0