The Egyptian Journal of Anatomy最新文献

{"title":"Effects of Hypothyroidism and Thyroxin Replacement on Postnatal Development of Lumbar Vertebrae in Albino Rats","authors":"R. Mohamed, Dorriea Abdallah Mohamed, Heba Mohamed, H. Hassanin","doi":"10.21608/ejana.2019.11222.1020","DOIUrl":"https://doi.org/10.21608/ejana.2019.11222.1020","url":null,"abstract":"Background: Normal skeletal development and adult bone maintenance requires normal level of thyroid hormones. Growth arrest, delayed bone maturation, and epiphyseal dysgenesis are fundamental result of hypothyroidism in children.     \u0000Aim of the work: To detect the effects of hypothyroidism and thyroxin treatment on the postnatal development of lumbar vertebrae in albino rats. \u0000 Materials and Methods: Sixty adult female rats were randomly subdivided into three equal groups; control group (received distilled water), carbimazole-treated group (received carbimazole in a dose of 6 microgram/gram body weight daily orally from 10th gestational day till the day 21 postnatally then carbimazole administration was continued to pups until the end of 8th week postnatally) and carbimazole+thyroxin treated group (received carbimazole on 10th gestational day in a dose of 6 microgram /gram body weight orally and thyroxin at birth in a dose of 10 microgram/kg/day orally and the administration of both drugs was continued until the day 21 postnatally then the administration was further continued to pups until the end of 8th week postnatally). The offsprings at ages (newborn, two weeks and eight weeks) anesthetized, sacrificed, their lumbar vertebrae were manibulated for histological examination. Morphometric and statistical studies were performed.   \u0000Results: The lumbar vertebrae markedly affected by hypothyroidism especially the proliferating zone and the collagen component of the matrix. Morphometric results revealed highly-significant decrease in the thickness of the epiphysis in hypothyroid rats. Most of these changes reverted by giving thyroxin. \u0000Conclusion: Vertebral column growth is markedly affected by hypothyroidism and thyroxin replacement has a protective role.","PeriodicalId":383073,"journal":{"name":"The Egyptian Journal of Anatomy","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128947209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The possible role of folic acid against sodium fluoride induced toxicity in the skin of adult male albino rats.","authors":"Heba Mohamed, Rasha I. Anwar","doi":"10.21608/ejana.2019.10703.1016","DOIUrl":"https://doi.org/10.21608/ejana.2019.10703.1016","url":null,"abstract":"Background:Exposure of human being to fluoride occurs through various sources as food, water, toothpastes and fluoride additives. This exposure is either to low concentrations for long periods or to high concentrations and both may lead to poisoning.  Folic acid (FA) has an important free radical scavenging property which makes it the most potent antioxidants. Moreover, it suppresses apoptosis and boost cell survival. \u0000Aim of the work: To evaluate the protective and therapeutic role of FA against sodium fluoride (NaF) induced toxicity on adult male rat skin. \u0000Materials and Methods:Forty adult (3 months) male albino rats(weighing 200-220 gm) were equally divided into 4 groups (10 rats each):group 1 (control),group 2 (NaF, for 4 weeks),group 3 (NaF, for 4 weeks followed by FA for another 4 weeks) and group 4 (NaF plus FA for 4 weeks). Both NaF (25mg/Kg b.w.) and FA (8 mg/Kg b.w.) were given orally daily. The rats were sacrificed and samples of the back skin were processed for histological, immunohistochemical (Bcl2 antiapoptotic protein) and morphometric studies. \u0000Results:Group 2 revealed signs of degeneration in epidermal cells, expansion of intercellular spaces and destructed organelles. There wasa reduction in epidermal thickness with disturbance in collagen fibers arrangement and weak immunoreactivity of Bcl-2. FA induced relative improvement in the dermal and epidermal structures in group 3 while there was preservation of these structures in group 4. \u0000Conclusion:FA ameliorates NaF induced harmful effects on skin in addition to its valuable preventive role.","PeriodicalId":383073,"journal":{"name":"The Egyptian Journal of Anatomy","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132827593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Histological Study of the Rat Duodenal Mucosa During Pre- and Post-weaning Periods","authors":"Haidy Farid Abdel Salam, S. Ali","doi":"10.21608/ejana.2019.11801.1022","DOIUrl":"https://doi.org/10.21608/ejana.2019.11801.1022","url":null,"abstract":"Background:   Changes in food constituent during suckling, weaning and post-weaning periods documented histological and functional adaptation in rat small intestine. \u0000Objectives: identify histological changing events, which represent such adaptation in the rat duodenal mucosa. \u0000 Material and methods: Twenty four male rat pups are divided into equal 4 groups including day one, 7 &15 (pre-weaning) and day 21 (weaning). In addition, two other groups including 12 male adult rats equally divided, aged 45 days (post-weaning) and 90 days, were used. The animals are euthanized at the fore mentioned days and the duodenum is processed for light and scanning electron microscopic examination. Paraffin sections are stained with haematoxylin & eosin and combined alcian blue/ PAS. Computer image analysis and statistical study are done for the height of the villi, depth of the crypts and villous / crypt ratio. \u0000 Results: The villi are broadening with the advance of age. The height of the villi   and the depth of the crypts show a statistical non-significant increase in the pre-weaning groups. At weaning, there is a significant decrease in the height of the villi with a decrease of the villous/crypt ratio. The villi are covered by brushed columnar enterocytes and some vacuolated enterocytes are seen only in pre-weaning period. Acidic and neutral mucin positive stained goblet cells are distributed on villi and within the crypts   of pre-weaning pups. At weaning, goblet cells are positive for acidic mucin only. Crypts are lined with pyramidal enterocytes and paneth cells are dominating in day 21. Both villi and crypts at postnatal day 21(weaning) acquire structural appearance similar to that of adult.","PeriodicalId":383073,"journal":{"name":"The Egyptian Journal of Anatomy","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130063812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential Protective Effect of Pentoxifylline on Myocardial Injury Induced by Ischemia Reperfusion in Rat Model: A Histological and Immuno-Histochemical Study.","authors":"Eman K. Habib, A. Hasanin","doi":"10.21608/ejana.2019.10605.1015","DOIUrl":"https://doi.org/10.21608/ejana.2019.10605.1015","url":null,"abstract":"Background: Although, myocardial reperfusion is a pre-requisite to salvaging viable myocardium, the process of restoring coronary blood flow can paradoxically induce myocardial injury and cardiomyocyte death. A number of new therapeutic strategies are currently under investigation for preventing myocardial reperfusion injury and to improve clinical outcomes. Aim of the work: This study was performed to assess the potential benefit of pentoxifylline in the reduction of myocardial injury induced by ischemia/reperfusion .  Material and Methods: In this study, 30 adult male albino rats were divided into 3 groups; control group, group II: subjected to ischemia (temporary occlusion of the left anterior descending coronary artery for 45 minutes) followed by reperfusion (for 120 minutes), and group III: single dose of pentoxifylline (40 mg/kg/bw) was injected intraperitoneal, 15 minutes before induction of ischemia/reperfusion. Samples from left ventricle from all animals were excised immediately, at the end of experimental time, and processed for examination by light and transmission electron microscopes. Also, immunohistochemical examination using caspase 3 and Anti TNF-α antibodies was performed. Results: Histological assessment revealed that the pretreatment with pentoxphilline protected the myocardium against ischemic/reperfusion induced injury; as demonstrated by improvement of histological structure of cardiac myocytes, minimal edema, hemorrage and fibrosis. No cellular inflammatory infiltration. Also, marked reduction of cardiomyocyte necrosis, indicating strong anti- apoptotic effect of pentoxphilline, and weak expression of TNF-α , indicating strong anti-inflammatory effect of pentoxphilline.","PeriodicalId":383073,"journal":{"name":"The Egyptian Journal of Anatomy","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116302877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Streptozotocin - Induced Diabetes on the Tongue of Adult Male Albino Rat and the Possible Protective Role of Cod Liver Oil","authors":"M. Isaac, E. Bekheet","doi":"10.21608/ejana.2019.7997.1007","DOIUrl":"https://doi.org/10.21608/ejana.2019.7997.1007","url":null,"abstract":"Introduction: Oral problems are among the common diabetes induced complications. Cod liver oil (CLO) is a rich source of polyunsaturated fatty acids that was found to provide a better control of glucose and lipid metabolism. Aim: to study the histological changes of streptozotocin (STZ) induced diabetes on rat’s tongue and evaluate the possible protective role of CLO. Material and methods: Thirty-six adult male albino rats, aging 4-6 months and weighting 180-200 gm, were used in this study. Animals were divided into three equal groups. Group I: was equally subdivided into three subgroups; subgroup IA: kept as negative control, subgroup IB: received single intraperitoneal injection of citrate buffer and Subgroup IC: received CLO by gastric tube daily for 12 weeks. Group II: received single intraperitoneal injection of STZ to induce diabetes. Group III: diabetes was induced as group II then rats were given CLO daily by gastric tube for 12 weeks. At the end of the experiment for each group, animals were anesthetized and tongues were dissected out and processed for light and scanning electron microscopic examination and immunohistochemical studies. Results: The present study revealed that diabetes induced marked irregularities, flattening and hyperkeratosis of tongue papillae in addition to distortion of the muscle layer. Group III revealed evident improvement of these changes. Conclusion: Obvious diabetes induced changes of tongue histoarchitecture were detected. However, CLO exerted a marked protective role against these changes. Thus, it could be a promising protector against diabetes induced tongue hazards.","PeriodicalId":383073,"journal":{"name":"The Egyptian Journal of Anatomy","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130887959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Hazards of Tramadol on the Liver Structure of Adult Male Albino Rats and the Possible Protective Role of N-acetylcysteine: Histological and Immunohistochemical Study","authors":"Marwa M Ahmad, A. Alsemeh, Hayam Rashed, Dalia El Shafei","doi":"10.21608/ejana.2019.251313","DOIUrl":"https://doi.org/10.21608/ejana.2019.251313","url":null,"abstract":"Background: Tramadol administration is associated with hepatic congestion, apoptosis and induces oxidative damage in tissues. The thiol group in N-acetyl-cysteine (NAC) interacts directly with reactive oxygen species leading to cellular protection against such damage. Aim of Work: The present study was designed to highlight the hazards of tramadol (TR) on liver structure of adult male albino rat and the efficacy of NAC on the histological structure, liver functions for protection against such possible induced hazards. Materials and Methods: Forty male albino rats were used. Rats were divided into four groups with 10 rats per each group. Control group received normal saline 2ml/day orally by gavage for 30 days. NAC-treated group received 150 mg/kg/day of NAC dissolved in normal saline by oral gavage for 30 days. TR- treated group received TR 40mg/kg/day dissolved in normal saline by oral gavage for 30 days. TR + NAC group were administered TR and NAC in similar doses as in TR and NAC groups' respectively for 30 days. The livers were removed and processed for light microscopic examination and different biochemical markers in addition to immunohistochemical examination of both Bax and 8- hydroxyl-2-deoxy guanosine (8-OHDG). Results: The TR- treated group revealed disrupted arrangement of the hepatic cords together with congestion of central vein and mononuclear perivascular infiltration. Most of the hepatocytes showed necrosis and apoptotic hepatocytes with bile duct duplication. There were diffuse fibrous tissue formation and a significant increase in the number of Bax and 8-OHdG positive cells. As regard to liver functions, there were highly significant elevations of serum levels of aspartate aminotransferase (AST), Alanine aminotransferase (ALT), Serum alkaline phosphatase (ALP) and bilirubin and reduction of serum albumin. As regard to the antioxidant enzymes, there was an increase of malondialdehyde (MDA) and decrease in superoxide dismutase (SOD), glutathione (GSH) and catalase (CAT) in the liver tissues. In contrast with TR+NAC group where liver parenchyma restored its uniform architecture with minimal collagen fibers, significant reductions in the number of Bax and 8-OHdG positive cells. Liver functions and antioxidant enzymes level nearly restored its normal values. Conclusion: The TR is suggested to have hepatotoxic effect and NAC might have protective effects against such induced hepatotoxicity.","PeriodicalId":383073,"journal":{"name":"The Egyptian Journal of Anatomy","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129251623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anatomical and Radiological Study of Adult Human Sacrotuberous Ligament and Its Relation to the Sacroiliac Joint","authors":"Dalia F. Kallini, M. Naguib, Enas Ahmed, Marwa Soliman","doi":"10.21608/ejana.2019.251315","DOIUrl":"https://doi.org/10.21608/ejana.2019.251315","url":null,"abstract":"Introduction: Low back pain (LBP) is an important problem that increased nowadays and affects quality of life. Sacroiliac joint (SIJ) is one of the important causes of LBP in about 15%_25% of the cases. Sacrotuberous ligament (STL) is a part of the fibromuscular network of the joint that may alter its biomechanics and become a diagnostic or management tool in dealing with LBP patients and sacroiliitis. Aim of the Work: The study aimed to give an anatomical and radiological review of the STL and correlate it with LBP and sacroiliitis. Materials and methods: Cadaveric study: 20 formalin fixed and 20 fresh frozen specimens of adult male human hip, gluteal and upper thigh were dissected exposing the STL and surrounding communications, also the pelvic cavity was dissected. The length and width of the ligament were measured and calculated as Mean ±SD. Radiological study: In a retrospective study, 35 pelvic reconstructed CT scans were collected from adult human male ranging from 20-40 years old and divided into three groups: 2 control groups, group (A): no LBP nor sacroiliitis, group (B): LBP but no sacroiliitis and group (C): LBP and sacroiliitis and this group was further subdivided into: group (C-): Normal STL and group (C+): Redundant free limb of the STL. Shape and length of STL in each group and measurements were statistically analyzed. Results: In cadaveric study: STL was divided to three limbs: two attached, one extending from posterior superior iliac spine to back of S3 and its length ranged from 20mm-35mm in formalin fixed specimens and from 32.9mm-44mm in fresh frozen specimens, and the other one extending from back of S3 to back of S5 and back of the coccyx and measured 28.1mm-38.4mm in formalin fixed specimens and from 35.2mm-45mm in fresh frozen specimens. The third limb (free limb) extending from the previous point to midpoint of medial surface of ischial tuberosity and its mean length was 45.21± 6.04mm in formalin fixed specimens and 52.17± 8.39mm in fresh frozen specimens. In radiological study: Redundancy of free limb of the STL was found in 10 out of 16 of CT scans with sacroiliitis with increased mean length up to 69.38 mm. Conclusion: Wide attachment of STL to the surrounding was observed and may affect SIJ biomechanics and increases probability of LBP. We recommend in further studies to consider STL in diagnosis and treatment of LBP patients and clinically evaluate the intensity of the condition and correlate it with the degree of the laxity of the ligament in CT scans.","PeriodicalId":383073,"journal":{"name":"The Egyptian Journal of Anatomy","volume":"2012 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127390269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Mercuric Chloride Exposure during Pregnancy and Lactation on the Postnatal Development of the Liver in the Albino Rat","authors":"M. Mohamed, M. El-Meligy, R. Bushra, Esraa Mohamed","doi":"10.21608/ejana.2019.251310","DOIUrl":"https://doi.org/10.21608/ejana.2019.251310","url":null,"abstract":"Background: Mercury (Hg) is a prominent environmental contaminant that causes detrimental effects to the human health. It is used in some thermometers, electrical switches, batteries, fluorescent lamps, paints, fungicides, insecticides and in mercuric vapours lamps. Mercury and its compounds have been also used in medicine as in topical antiseptics, stimulant laxatives, skin lightening products, diaper rash ointment, eye and nasal sprays. Elemental mercury is an ingredient in dental amalgams. Thiomersal (mercury-based preservative) is an organic compound that is used as a preservative in vaccines and in the manufacture of mascara. Aim of the Work: To detect the effects of mercuric chloride (HgCl 2 ) exposure during pregnancy and lactation on the postnatal development of the liver in albino rat. Materials and Methods: A total number of sixteen pregnant albino rats were used in the study. They were equally divided into control and experimental groups. During the whole periods of gestation and lactation, the control females received an oral daily saline of 2 mg /kg body weight. The experimental females received an oral daily dose of 2 mg HgCl 2 /kg body weight. After weaning, the offspring of the treated group was given HgCl 2 of the same oral daily dose. The control and treated mothers’ offspring was sacrificed at the following ages: 1 day (group I), 21 days (group II) and 2 months (group III). Each group consisted of 6 rats. At the time of scarification, the rats were weighed, anaesthetized and the livers were extracted and weighed. The specimens from the fixed livers were dissected and processed for the light and the electron microscopic examination. Morphometric studies were also done. Results: Light microscopic study of the treated groups revealed vacuolization, degeneration of the hepatocytes, inflammatory cell infiltration, dilated and congested hepatic sinusoids as well as portal venules. Weak PAS reaction was observed in the treated liver specimens of groups I and II and a strong PAS reaction in the treated group III when compared with the corresponding controls. The electron microscopic study showed degeneration of the mitochondria, vacuolization of the cytoplasm, congested sinusoids with perisinusoidal fibrosis. Morphometric studies revealed a significant increase in the liver weight inspit of the decrease in the body weight. Conclusion: Ingestion of HgCl 2 during pregnancy and lactation produces hepatic affections of the offspring.","PeriodicalId":383073,"journal":{"name":"The Egyptian Journal of Anatomy","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124556996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Effects of Allium Cepa on Testis of Rats Exposed to GlyPhosate","authors":"N. Sarg, Samia M. Manawy, K. Kamal","doi":"10.21608/ejana.2019.251309","DOIUrl":"https://doi.org/10.21608/ejana.2019.251309","url":null,"abstract":"Background: Glyphosate (N-(phosphonomethyl)glycine) is one of the most widely used organophosphorus herbicides. Allium cepa (AcE), popularly known as onion, has been reported to have an antioxidant properties in both rats and human. Aim of Study: Is to investigate the histological, and immunohistochemical effects of Glyphosate (GP) as an Organophosphrous compound (OPC) on rat testis and to assess the protective effect of Allium cepa on testis of rats exposed to glyphosate. Materials and Methods: This study included 30 adult male Albino rats divided into 3groups. Group I (Control group): Each rat received distilled water(0.2 ml/day). Group II: Each rat received glyphosate at a dose of 125 mg /kg, body weight. Group III: Each rat was given Allium cepa (AcE) 1 ml/100 g BW two hours before administration of GP at a dose of 125 mg /kg body weight. All the drugs as well as distilled water were given daily by oral gavages for 30days. Results: The study had demonstrated that glyphosate caused a degeneration of all layers of the germ cells, congestion of the blood vessels, and increased of the collagen fibers in the capsule . Immunohistochemical results showed a decrease in the expression of the antiapoptotic protein(Bcl2). Allium cepa administration partially ameliorated the degeneration effect of glyphosate on the seminiferous tubules. Conclusion: Allium Cepa protects the testis from the toxic effect of glyphosate.","PeriodicalId":383073,"journal":{"name":"The Egyptian Journal of Anatomy","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121899961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Histological Study to Evaluate the Efficacy of the Antidepressant Fluoxetine Versus the Anti-inflammatory Sulfasalazine in Experimentally Induced Colitis in Adult Male Albino Rats","authors":"A. Soliman, Dalia F. Kallini, Rania A. Salah El Din, H. A. Abdel Hamid, S. Kamar, Shimaa S. Mohammed","doi":"10.21608/ejana.2019.251311","DOIUrl":"https://doi.org/10.21608/ejana.2019.251311","url":null,"abstract":"Background: The growing prevalence of the inflammatory bowel diseases in Arab community increases both economic and health care burden. Thus, better and more affordable treatment cure is greatly needed. Aim of the Work: T he goal of work was to study the histological effect of the antidepressant drug (fluoxetine) versus the traditional anti-inflammatory drug (sulfasalazine) on induced colitis in adult male albino rats. Material and Methods: Twenty-four adult male albino rats were randomly divided into four groups, each containing six rats.: group (A) which served as the control group; group (B), in which colitis was induced by intra-rectal administration of 1 ml of 2% acetic acid daily for 3 consecutive days then left without any treatment for 14 days; group (C), in which the rats received sulfasalazine for 14 days after induction of colitis as in group B; and group (D) in which the rats received fluoxetine 14 days after induction of colitis. Both drugs in group (C) and (D) were dissolved in distilled water and administered by oral gavage once daily. At the end of the experiment, distal colon (10 cm proximal to the anus) was removed and processed for light microscopic examination using H & E, combined alcian blue – PAS and Mallory’s trichrome stains. Toluidine blue stain was used for semi-thin sections. Computer image analysis and statistical study were also performed for the number of goblet cells and area percent of collagen fibers content. Results: Colitis induction showed mucosal injury. There was loss of surface epithelium and disruption of crypt architecture. The muscularis mucosae showed vacuolations. Moreover, there were dilated blood vessels in submucosa. Administration of fluoxetine improved the colonic structure especially the surface epithelium while sulfasalazine was by far less efficient in improving induced colitis. Conclusion: Fluoxetine improved the colonic structure and proved to be more effective management of experimentally induced colitis with less adverse effects than the conventional management by sulfasalazine.","PeriodicalId":383073,"journal":{"name":"The Egyptian Journal of Anatomy","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128450814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}