EuPA Open Proteomics最新文献_第4页

Public proteomics data: How the field has evolved from sceptical inquiry to the promise of in silico proteomics 公共蛋白质组学数据:该领域如何从怀疑的调查发展到计算机蛋白质组学的前景

EuPA Open Proteomics Pub Date : 2016-06-01 DOI: 10.1016/j.euprot.2016.02.005

Lennart Martens

引用次数: 5

Proteomics: Back to the future 蛋白质组学:回到未来

EuPA Open Proteomics Pub Date : 2016-06-01 DOI: 10.1016/j.euprot.2016.04.001

Paola Roncada (Guest editor), Gabriella Tedeschi, Mauro Fasano (Guest editor), Peter Verhaert (Editor in chief)

引用次数: 0

Peptide-mediated ‘miniprep’ isolation of extracellular vesicles is suitable for high-throughput proteomics 肽介导的细胞外囊泡的“迷你”分离适用于高通量蛋白质组学

EuPA Open Proteomics Pub Date : 2016-06-01 DOI: 10.1016/j.euprot.2016.02.001

Jaco C. Knol , Inge de Reus , Tim Schelfhorst , Robin Beekhof , Meike de Wit , Sander R. Piersma , Thang V. Pham , Egbert F. Smit , Henk M.W. Verheul , Connie R. Jiménez

{"title":"Peptide-mediated ‘miniprep’ isolation of extracellular vesicles is suitable for high-throughput proteomics","authors":"Jaco C. Knol , Inge de Reus , Tim Schelfhorst , Robin Beekhof , Meike de Wit , Sander R. Piersma , Thang V. Pham , Egbert F. Smit , Henk M.W. Verheul , Connie R. Jiménez","doi":"10.1016/j.euprot.2016.02.001","DOIUrl":"10.1016/j.euprot.2016.02.001","url":null,"abstract":"<div><p>Extracellular vesicles (EVs) are cell-secreted membrane vesicles enclosed by a lipid bilayer derived from endosomes or from the plasma membrane. Since EVs are released into body fluids, and their cargo includes tissue-specific and disease-related molecules, they represent a rich source for disease biomarkers. However, standard ultracentrifugation methods for EV isolation are laborious, time-consuming, and require high inputs. Ghosh and co-workers recently described an isolation method utilizing Heat Shock Protein (HSP)-binding peptide Vn96 to aggregate HSP-decorated EVs, which can be performed at small ‘miniprep’ scale. Based on microscopic, immunoblot, and RNA sequencing analyses this method compared well with ultracentrifugation-mediated EV isolation, but a detailed proteomic comparison was lacking. Therefore, we compared both methods using label-free proteomics of replicate EV isolations from HT-29 cell-conditioned medium. Despite a 30-fold different scale (ultracentrifugation: 60ml/Vn96-mediated aggregation: 2ml) both methods yielded comparable numbers of identified proteins (3115/3085), with similar reproducibility of identification (72.5%/75.5%) and spectral count-based quantification (average CV: 31%/27%). EV fractions obtained with either method contained established EV markers and proteins linked to vesicle-related gene ontologies. Thus, Vn96 peptide-mediated aggregation is an advantageous, simple and rapid approach for EV isolation from small biological samples, enabling high-throughput analysis in a biomarker discovery setting.</p></div>","PeriodicalId":38260,"journal":{"name":"EuPA Open Proteomics","volume":"11 ","pages":"Pages 11-15"},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.euprot.2016.02.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36220633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

A novel preparation technique of red (sparkling) wine for protein analysis 蛋白质分析用红葡萄酒(起泡酒)制备新工艺

EuPA Open Proteomics Pub Date : 2016-06-01 DOI: 10.1016/j.euprot.2016.03.001

Elisabeth I. Vogt , Veronika M. Kupfer , Rudi F. Vogel, Ludwig Niessen

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引用次数: 13

EuPACa: An Opportunity for EuPA to co-ordinate and promote Prostate Cancer Proteomics in Europe as part of Global Initiatives EuPACa:作为全球计划的一部分，EuPA在欧洲协调和促进前列腺癌蛋白质组学的机会

EuPA Open Proteomics Pub Date : 2016-06-01 DOI: 10.1016/j.euprot.2016.03.012

Corinna Henkel , Stephen R Pennington , Andrea Urbani

引用次数: 0

The Serbian Proteomics Association (SePA) 塞尔维亚蛋白质组学协会(SePA)

EuPA Open Proteomics Pub Date : 2016-06-01 DOI: 10.1016/j.euprot.2016.03.013

Tanja Ćirković Veličković , Tatjana Simić , Svetlana Dinić

引用次数: 0

Experimental setup for the identification of mitochondrial protease substrates by shotgun and top-down proteomics 采用鸟枪法和自上而下的蛋白质组学方法鉴定线粒体蛋白酶底物的实验装置

EuPA Open Proteomics Pub Date : 2016-06-01 DOI: 10.1016/j.euprot.2016.02.002

Alice Di Pierro , Heather Bondi , Chiara Monti , Luisa Pieroni , Enrico Cilio , Andrea Urbani , Tiziana Alberio , Mauro Fasano , Maurizio Ronci

引用次数: 2

Systemic amyloidoses and proteomics: The state of the art 系统性淀粉样变性和蛋白质组学:最新进展

EuPA Open Proteomics Pub Date : 2016-06-01 DOI: 10.1016/j.euprot.2016.02.003

Francesca Lavatelli , Andrea di Fonzo , Giovanni Palladini , Giampaolo Merlini

引用次数: 16

The EuPA Standardization Initiative EuPA标准化倡议

EuPA Open Proteomics Pub Date : 2016-06-01 DOI: 10.1016/j.euprot.2016.03.007

F. Canals , F. Elortza , A. Paradela , G. Corthals , Michelle Camenzuli , A. Muñoz , O. Schiltz , Anne Gonzalez de Peredo , A Sickman , C. Borchers , Fernando J. Corrales

引用次数: 0

Call for Candidates for the 2016 EuPA Young Investigator Prize (PhD) 2016年EuPA青年研究者奖(博士)候选人征集

EuPA Open Proteomics Pub Date : 2016-06-01 DOI: 10.1016/j.euprot.2016.03.010

Karl Mechtler

引用次数: 0