Meta Gene最新文献

{"title":"Identification of hub genes associated with human osteoarthritis cartilage: An in silico approach","authors":"Swetha Sunkar,&nbsp;K. Namratha,&nbsp;Desam Neeharika","doi":"10.1016/j.mgene.2022.101015","DOIUrl":"10.1016/j.mgene.2022.101015","url":null,"abstract":"<div><p>Osteoarthritis is a common orthopedic disease among the greatest causes of morbidity and disability worldwide; however, research on its pathogenesis and diagnostic methods remains limited. The present study focuses on analyzing the microarray dataset to elucidate the hub genes and pathways related to the osteoarthritis. The gene expression data was retrieved from GEO database (GSE169077) and differentially expressed genes were determined using GEO2R tool based on adjusted <em>P</em>-value and log2FC values based on which 27 genes were found to be significant of which 9 genes were up-regulated while 18 genes were down-regulated. This was followed by gene enrichment analysis identify the related Gene Ontology terms and pathways. The Protein-Protein Network is constructed with 15 nodes and 22 edges using STRING database and then exported to Cytoscape 3.8 to predict the hub genes. The hub genes identified are <em>POSTN, COL1A2, COL1A1, BMP1, MXRA5, MMP13 and SERPINF1</em>. The hub genes identified were not only found to be associated with bone related disorders but also few were involved in other diseases. Therefore targeting these genes for disease management could be a viable option in osteoarthritis.</p></div>","PeriodicalId":38190,"journal":{"name":"Meta Gene","volume":"31 ","pages":"Article 101015"},"PeriodicalIF":0.7,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48584541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Linkage and association of PAX7 polymorphisms (rs742071, rs766325, and rs4920520) with the risk of non-syndromic cleft lip with/without cleft palate: A systematic review and meta-analysis","authors":"Mohammad Moslem Imani ,&nbsp;Rahil Rahimi ,&nbsp;Masoud Sadeghi","doi":"10.1016/j.mgene.2021.101007","DOIUrl":"10.1016/j.mgene.2021.101007","url":null,"abstract":"<div><p>The studies have reported several additional non-syndromic cleft lip/palate (NSCL/P) susceptibility loci. This systematic review and meta-analysis aimed to evaluate the linkage and association of <em>PAX7</em><span> polymorphisms with the NSCL/P risk. A comprehensive search was conducted in the PubMed, Cochrane Library<span>, Web of Science, and Scopus databases until May 15, 2021. The association between </span></span><em>PAX7</em><span> polymorphisms and NSCL/P susceptibility was analyzed by calculation of the odds ratios (ORs) and 95% confidence intervals (CIs), and the linkage was assessed by the allelic transmission disequilibrium test. Some articles included more than one study (reporting more than one polymorphism). Therefore, nine articles including 13 studies were entered into the meta-analysis. With regard to the association of </span><em>rs742071</em> polymorphism with the risk of NSCL/P, the pooled OR was 1.33 for the allelic (<em>P</em> &lt; 0.0001), 1.98 for the homozygous (<em>P</em> = 0.0007), 1.41 for the heterozygous (<em>P</em> = 0.0267), and 1.15 for the dominant (<em>P</em> = 0.2542) models. For the association of <em>PAX7 rs766325</em> and <em>rs4920520</em> polymorphisms with the risk of NSCL/P, the pooled OR was 0.96 (<em>P</em> = 0.5571) and 1.14 (<em>P</em> = 0.1020), respectively. In addition, the pooled allelic transmission disequilibrium test for <em>s742071</em> and <em>rs766325</em> polymorphisms did not show any allelic linkage between these polymorphisms and susceptibility to NSCL/P. The main results of the present systematic review and meta-analysis showed an association between <em>PAX7 rs742071</em> polymorphism and NSCL/P susceptibility; but there was no linkage or association with <em>rs766325</em> and <em>rs4920520</em> polymorphisms.</p></div>","PeriodicalId":38190,"journal":{"name":"Meta Gene","volume":"31 ","pages":"Article 101007"},"PeriodicalIF":0.7,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46215954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Are the genetic variants/haplotypes of the CDH1 gene contribute to skin tags and internal malignancies in skin tag subjects? A pilot study","authors":"Noha Rabie Bayomy ,&nbsp;Suzy Fawzy Gohar ,&nbsp;Reem Ahmed Abd El-Aziz ,&nbsp;Amira Ibrahim Aldesoky ,&nbsp;Nashwa Mahmoud Mouhamed Muharram","doi":"10.1016/j.mgene.2022.101011","DOIUrl":"10.1016/j.mgene.2022.101011","url":null,"abstract":"<div><h3>Background</h3><p>What is the etiology of skin tags? What factors are involved in the patho-biochemistry of combined skin tags and internal malignancy? This study aimed to assess the possible linkage between <em>CDH1</em> gene polymorphisms and skin tags and whether certain <em>CDH1-SNPs</em> are the key to developing internal malignancies in the subjects with skin tags.</p></div><div><h3>Materials and methods</h3><p><em>CDH1</em> polymorphisms were assessed in 164 skin tag subjects and 126 controls. We followed up the subjects with skin tags every six months for 48 months to assess whether anyone developed internal malignancy and if that malignancy was linked to certain <em>CDH1</em> polymorphism.</p></div><div><h3>Results</h3><p><em>CDH1</em>rs13689 genotypes were T/T (1.2% vs 4.8%), T/C (46% vs 60%) and C/C (70.7% vs 47.6%) for the skin tag subjects and the controls, respectively (<em>p</em> &lt; 0.001). The <em>CDH1</em>rs17715799 genotypes were A/A (29.3% vs 50.8%), A/T (56.1% vs 44.4%) and T/T (14.6% vs 4.8%) for the skin tag subjects and the controls, respectively, (<em>p</em> &lt; 0.001). After follow up, six patients with skin tags developed internal malignancies.</p></div><div><h3>Conclusions</h3><p><em>CDH1</em><span> genetic variants may be incriminated in the process of skin tag formation through epithelial-mesenchymal transition (EMT). Obesity and diabetes mellitus (DM) are substantial risk factors for EMT. Internal malignancy might develop with skin tags, so these patients should be followed up. The mutant </span><em>CDH1</em>rs13689 and/or rs17715799 may be the key to the presence of combined skin tags and malignancy.</p></div>","PeriodicalId":38190,"journal":{"name":"Meta Gene","volume":"31 ","pages":"Article 101011"},"PeriodicalIF":0.7,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41836446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular profiles of predictive biomarkers for platinum-based chemotherapy in Non-Small Cell Lung Cancer (NSCLC)","authors":"NiloofarTaleghani Seyedabadi ,&nbsp;Sara YousefZadeh Shoushtari ,&nbsp;Asma Soofi ,&nbsp;Javad Arabpour ,&nbsp;Zinat Shams ,&nbsp;Homa Akhavan ,&nbsp;Saied Hosseini-Asl","doi":"10.1016/j.mgene.2021.100993","DOIUrl":"10.1016/j.mgene.2021.100993","url":null,"abstract":"<div><h3>Background</h3><p>Non-small cell lung cancer (NSCLC) is the principal subtype of lung cancer. Among all therapeutic options, platinum-based chemotherapy agents, especially Cisplatin<span>, are still commonly used treatment for NSCLC patients. However, developing chemoresistance in NSCLC cells often gives rise to chemotherapy failure. Therefore, more studies are required to shed light on gene interaction and cellular pathways involved in initiating and developing resistance to platinum-based chemotherapy in NSCLC. Hence, it is urgent to find the key genes, microRNA (miRNAs), and potential molecular mechanisms implicated in chemoresistance and present markers to predict response to platinum-based chemotherapy in NSCLC patients.</span></p></div><div><h3>Methods</h3><p>The microarray datasets GSE6410, GSE7035, GSE14814, GSE26704, GSE73302 were downloaded from the Gene Expression Omnibus (GEO) database and were analyzed using R software. Functional and pathway enrichment analyses were performed using the Enrich R site. Then, the protein-protein interaction (PPI) network and hub genes<span> were obtained using the Cytoscape software. Further, the miRSystem database was performed to predict the miRNAs regulating the hub genes. Moreover, Cytoscape software and the CytoHubba plugin were used to construct the miRNA-target interaction network and hub modules. Finally, the Kaplan–Meier curve was used to demonstrate the survival curves and assess the association of the genes signature with clinical outcomes.</span></p></div><div><h3>Results</h3><p><span>A total of 142 differentially expressed genes (DEGs) were found. The gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses present the p53 </span>signaling pathway as the most significant pathway.</p><p><span>Besides, from the top ten terms obtained of Biological Process<span>, Molecular Function, and Cellular Component, the first ones, including cholesterol biosynthetic process, the extrinsic component of external side of plasma membrane, cytokine activity, were selected respectively. Based on the PPI network, the ten nodes with the highest degree were screened as hub genes. In addition, from the miRNA–target regulatory network in Cytoscape, ten hub nodes were found. Ultimately, according to Kaplan–Meier curve, BTG2 and TP53I3 with </span></span><em>p</em>-value &lt;0.05 were associated with a better prognosis.</p></div><div><h3>Conclusions</h3><p>In the present study, DEGs, candidate miRNAs, and underlying mechanisms involved in chemoresistance were identified to suggest potential biomarkers to provide new clues for the prediction of response to platinum-based chemotherapy.</p></div>","PeriodicalId":38190,"journal":{"name":"Meta Gene","volume":"31 ","pages":"Article 100993"},"PeriodicalIF":0.7,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45941555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is there any relationship between serum zinc levels and angiotensin-converting enzyme 2 gene expression in patients with coronavirus disease 2019?","authors":"Zahra Bagheri-Hosseinabadi ,&nbsp;Ali Pirsadeghi ,&nbsp;Amir Rahnama ,&nbsp;Fatemeh Bahrehmand ,&nbsp;Mitra Abbasifard","doi":"10.1016/j.mgene.2021.100991","DOIUrl":"10.1016/j.mgene.2021.100991","url":null,"abstract":"<div><h3>Background</h3><p>The level of angiotensin-converting enzyme 2 (ACE2) expression in different tissues is essential in the sensitivity, symptoms and consequences of COVID-19 infection. It seems that zinc is involved in the structure of the ACE2 enzyme has been identified; nonetheless, the relationship between <em>ACE2</em> expression and zinc serum levels in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-infected patients is still unclear. This study aimed to evaluate the expression of <em>ACE2</em> in peripheral blood-derived immune cells of COVID-19 patients and its relationship with serum zinc levels.</p></div><div><h3>Methods</h3><p>Thirty healthy subjects and thirty patients with COVID-19 were enrolled in this study. The COVID-19 infection was confirmed by positive real-time polymerase chain reaction (RT-PCR) and radiological data. Peripheral blood samples were taken from healthy subjects and COVID-19 patients. Whole blood samples were also used to measure <em>ACE2</em> gene expression by RT-PCR technique. The correlation matrix evaluated the relationship between <em>ACE2</em> expression, serum zinc levels, and other related variables.</p></div><div><h3>Results</h3><p>The outcomes showed no considerable alteration in serum zinc levels between patients and the control group. Likewise, the <em>ACE2</em> gene expression results showed a significant decrease in this receptor's expression in COVID-19 patients compared with the healthy subjects. A significant positive correlation was observed between serum zinc level and <em>ACE2</em> gene expression in patients with COVID-19.</p></div><div><h3>Conclusion</h3><p>The immune system seems to reduce the mRNA expression of the <em>ACE2</em> in the peripheral blood leukocytes following SARS-CoV-2 infection. Moreover, zinc deficiency can make patients more susceptible to SARS-CoV-2 infection.</p></div>","PeriodicalId":38190,"journal":{"name":"Meta Gene","volume":"31 ","pages":"Article 100991"},"PeriodicalIF":0.7,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8572017/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39732088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of CTLA4 c.49A > G (rs231775; p.Thr17Ala) gene variant with the risk of hepatocellular carcinoma and gastric cancer: A meta-analysis and meta-regression","authors":"Akram Abbas El Awady ,&nbsp;Rami M. Elshazli ,&nbsp;Ahmed Akram El Awady ,&nbsp;Abdelaziz Elgaml ,&nbsp;Ahmed K. Khalifa ,&nbsp;Ahmad Settin","doi":"10.1016/j.mgene.2021.100999","DOIUrl":"10.1016/j.mgene.2021.100999","url":null,"abstract":"<div><h3>Objective</h3><p>Various reports have examined the contribution of the <em>CTLA4 c.49A</em> <em>&gt;</em> <em>G (rs231775; p.Thr17Ala)</em><span> gene variant with different cancerous disorders. This meta-analysis was executed to further probe into the involvement of this missense variant with the susceptibility for hepatocellular carcinoma (HCC) and gastric cancer (GC).</span></p></div><div><h3>Methodology</h3><p>Following a wide-based scrutinized search of the internet done by three independent researchers for the contribution of the <em>CTLA4 c.49A</em> <em>&gt;</em> <em>G (rs231775; p.Thr17Ala)</em><span> variant with the cancer risk up to February 2021, only 16 case-control studies were found relevant and usable to the analysis out of 575 total retrieved reports. Multiple genetic models were checked for the proposed association through the computation of the odds ratio (OR) in addition to their 95% confidence intervals (95%CIs). Stratification and regression analysis was also carried out for the analyzed reports based on their geographical distributions, genotyping techniques, source of cancer-free controls, genetic equilibrium within cancer-free controls, and quality score. In addition, trial sequential analysis (TSA) was applied to test for the adequacy of the total sample size.</span></p></div><div><h3>Results</h3><p>This meta-analysis has included 4320 HCC and GC patients in conjunction with 6601 cancer-free controls. This work disclosed a significant association for the <em>CTLA4 c.49A</em> <em>&gt;</em> <em>G (rs231775; p.Thr17Ala)</em> variant with HCC among overall subjects tested by the recessive model [OR = 1.235, 95% CI = 1.050–1.453, <em>P-value</em> = 0.011]. Similarly, an elevated risk of GC was noticed associated with this variant within the overall subjects tested by the allelic model [OR = 1.225, 95% CI = 1.070–1.401, <em>P-value</em> = 0.003], and dominant model [OR = 1.352, 95% CI = 1.081–1.691, <em>P-value</em> = 0.008]. Furthermore, the stratification analysis showed a verification of correlation for this variant with HCC in Asian subjects under the recessive model, while the association was observed to be significant with GC in Asian and Caucasian patients under the dominant model. TSA confirmed that this work had significant findings noting that the collective <em>Z</em>-curve spanned the examining borderlines prior to attaining sample size confirming the acceptability of the study sample size.</p></div><div><h3>Conclusion</h3><p><em>The CTLA4 c.49A</em> <em>&gt;</em> <em>G (rs231775; p.Thr17Ala)</em><span> gene variant could be considered as an actual risk factor for the susceptibility of HCC and GC warranting efficient and adequate genetic counseling for this gene variant carriers.</span></p></div>","PeriodicalId":38190,"journal":{"name":"Meta Gene","volume":"31 ","pages":"Article 100999"},"PeriodicalIF":0.7,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48279495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of the associations of the T-786C and Glu298Asp polymorphisms of the eNOS gene as risk factors in the rupture of intracranial aneurysms","authors":"Marta Marques de Carvalho Lopes ,&nbsp;Hugo Alberto Rojas ,&nbsp;Luiza Franklin Polizzi ,&nbsp;Kênia Cristina S.F. Magalhães ,&nbsp;Valéria Cristina Sandrim ,&nbsp;Marcos Dellaretti ,&nbsp;Fernando Victor Martins Rubatino ,&nbsp;Renata Toscano Simões","doi":"10.1016/j.mgene.2021.101003","DOIUrl":"10.1016/j.mgene.2021.101003","url":null,"abstract":"<div><h3>Summary</h3><p><span>The mechanisms involved in aneurysmal etiology are complex and only partially understood. Genetic risk factors have already been related to the process of aneurysm rupture. Among the genetic factors, the T-786C and Glu298Asp polymorphisms of the </span><span><em>eNOS</em></span><span> gene have great clinical relevance, as they can affect the bioavailability of nitric oxide for the cerebrovascular system.</span></p></div><div><h3>Objective</h3><p>To evaluate the relationship between <em>eNOS</em> T-786C and Glu298Asp polymorphisms and the aneurysm pathogenesis of patients seen in the Neurosurgery Department of Santa Casa de Belo Horizonte, as well as to compare them with sociodemographic characteristics and risk factors.</p></div><div><h3>Methods</h3><p>A total of 211 whole blood samples were collected from patients with cerebral aneurysms, 160 with ruptured aneurysms, 51 with unruptured aneurysms and 215 controls. After DNA extraction, genotyping was performed using the PCR-RFLP technique. Allele and genotype frequencies were obtained using the GENEPOP 4.2 software, and statistical analysis was performed using the GraphPad Prism 5.0 program and RStudio version 1.4.</p></div><div><h3>Results</h3><p>Age, female sex, smoking and small diameter of the aneurysms were associated with aneurysm development and rupture in the study population. The CC genotype of the T-786C polymorphism was associated with unruptured aneurysms with a diameter &gt;12 mm.</p></div><div><h3>Conclusion</h3><p>Age, female sex and smoking were associated with rupture. This study revealed that the CC mutant genotype of the <em>eNOS</em> gene T-786C polymorphism was associated with unruptured intracranial aneurysms larger than 12 mm in our study population, revealing a new association.</p></div>","PeriodicalId":38190,"journal":{"name":"Meta Gene","volume":"31 ","pages":"Article 101003"},"PeriodicalIF":0.7,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43899553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autoimmune thyroid patients with CTLA-4 (+49A/G) GG/AG genotypes have high seropositivity to thyroid peroxidase than thyroglobulin","authors":"Nusrath Fathima ,&nbsp;Qursheed Sultana ,&nbsp;Syyeda Anees ,&nbsp;Kaleem Ullah ,&nbsp;Vitaly Ryu ,&nbsp;Aleem Ahmed Khan ,&nbsp;Mohammed Ishaq","doi":"10.1016/j.mgene.2022.101010","DOIUrl":"10.1016/j.mgene.2022.101010","url":null,"abstract":"<div><p><span>Despite increasing burden of autoimmune thyroid disease (AITD) in India, there is paucity of data for the genetic susceptibility<span> in Indian population which represents an important aspect to uncover the genetic factors for the cause or progression of different forms of AITD. It is known that production of auto-antibodies to </span></span>thyroglobulin<span><span> (TG) and thyroid peroxidase (TPO) is a characteristic feature of AITD. Production of high titres of auto-antibodies against TG and TPO is one the hallmarks of AITD that often precedes the development of clinical disease. The gene coding for cytotoxic T lymphocyte antigen-4 (CTLA-4) is considered an important candidate gene for susceptibility to AITD and also determines seropositivity. Earlier studies have demonstrated that polymorphic variants in CTLA-4 gene are linked to determine seropositivity. Hence, this study investigates a relationship between CTLA-4 (+49A/G) genotypes and seropositivity to anti-TPO and anti-TG antibodies in AITD patients. A total of 85 Indian participants were included and genotyped for CTLA-4 (+49A/G) </span>single nucleotide polymorphism<span> and further analyzed for seropositivity of TPO and TG auto-antibodies in mean age group of 35.76 ± 12.42 years of both the genders. The TPO seropositivity was reported higher in G allele containing genotypes GG and AG (66.66% and 58%, respectively) compared to AA (44.8%) genotype. Whereas, TG auto-antibodies showed highest seropositivity for genotype AA (55.17%) compared to AG (36%) and GG (16.6%). Higher median titre values were observed for TPO antibodies with GG genotype (1587.461 U/mL). In contrast to this, higher titres of auto-antibodies to TG were observed for individuals with AA genotype (520.746 U/mL). In conclusion, this study demonstrates a relationship between CTLA4 (+49A/G) genotype and seropositivity to thyroid auto antigens TPO and TG in Indian patients with clinical manifestation of Hashimoto's thyroiditis and Graves' disease, two different forms of AITD.</span></span></p></div>","PeriodicalId":38190,"journal":{"name":"Meta Gene","volume":"31 ","pages":"Article 101010"},"PeriodicalIF":0.7,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47053376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of sex hormone-binding globulin gene polymorphisms on the serum level of SHBG hormone in the men with prostate cancer","authors":"Zahra Tahmasebi Fard","doi":"10.1016/j.mgene.2021.101000","DOIUrl":"10.1016/j.mgene.2021.101000","url":null,"abstract":"<div><p><span>To evaluated the possible correlations of prostate cancer and three coding region polymorphisms (rs 6257, rs6258, rs 6259), &amp; changing repeat of TAAAA in promoter of SHBG gene regarding to concentration of Prostate specific Antigen and SHBG levels. For study purpose, a total of 352 subjects (176 patients and 176 controls) were recruited. The </span>ELISA<span> technique and the RFLP-PCR method were used. Logistic and adjusted regression of genotypes showed that the (TAAAA)n polymorphism in individuals carrying more than five repeats of the five-nucleotide sequence of TAAAA, as well as AA+AG (rs6257) and AA+AG (rs6259), increased the risk of prostate cancer by the chance ratio of 1.622, 2.005, and 2.469, respectively. The studied polymorphisms showed a significant relationship with the stage of the disease and Gleason score. The individuals carrying the mutants were less likely to develop prostate cancer than the ones with wild genotypes. All the genotypes in the cancer group had higher serum levels of tPSA and fPSA than those in the control group. A significant association was observed (except tPSA and fPSA for GT (rs6258) and fPSA for GA (rs6259)). Serum level of SHBG also showed a significant correlation with prostate cancer in genotypes &gt;Panta TAAAA (TAAAA)n, GG (rs6257), TT and TT + CT (rs6258), and GG, AA, and AA+GA (rs6259). These polymorphisms by changing the serum levels SHBG contributed to the incidence of prostate cancer.</span></p></div>","PeriodicalId":38190,"journal":{"name":"Meta Gene","volume":"31 ","pages":"Article 101000"},"PeriodicalIF":0.7,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42025419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of interferon-induced helicase (IFIH1) gene polymorphism rs1990760 with type two diabetes mellitus in Iraqi population","authors":"Suhad Rasheed Majeed ,&nbsp;Ali M. Omara ,&nbsp;Dhafer A.F. Al-Koofee","doi":"10.1016/j.mgene.2021.100952","DOIUrl":"10.1016/j.mgene.2021.100952","url":null,"abstract":"<div><h3>Background</h3><p>Type 2 diabetes mellitus (T2DM) is the most common type of diabetes. It is caused by inefficient insulin use, as well as the progressive loss of pancreatic β cells function. T2DM is the most common type of diabetes in the world. It is the major cause of death in adults and children, mainly of heart disease. The non-synonymous polymorphism rs1990760 is located in the HNF-3b binding site within exon 15 of the <em>IFIH1</em> coding region. At codon 946, it encodes a change from alanine to threonine (Ala 946 Thr).</p></div><div><h3>Methods</h3><p>On 100 patients and 100 controls, a case-control study was conducted. After extraction of genomic DNA, the SNP rs 1,990,760 analysis was performed using Taq-man real time PCR (RT-PCR). Fasting blood sugar, insulin level, HOMA-IR and lipid profile were determined. Anthropometric parameters such as height, weight and BMI were calculated. T2DM risk was determined as an odds ratio.</p></div><div><h3>Results</h3><p>TT, CT genotype carriers were less likely to develop T2DM [OR = 0.38, CI 95% = 0.17–0.82, <em>P</em> = 0.01] and [OR = 0.40, CI 95% = 0.16–5.99, <em>P</em> = 0.04] respectively.</p><p>High density lipoprotein (HDL) is shown significant differences in codominant model among genotypes in the patient's group.</p></div><div><h3>Conclusion</h3><p>Interferon-induced helicase (<em>IFIH1</em>) gene polymorphism rs1990760 is involved in reduced risk of T2DM. In addition this SNP may play a role in the development of cardiovascular diseases by affecting HDL levels.</p></div>","PeriodicalId":38190,"journal":{"name":"Meta Gene","volume":"30 ","pages":"Article 100952"},"PeriodicalIF":0.7,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.mgene.2021.100952","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45303227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}