Autoimmune thyroid patients with CTLA-4 (+49A/G) GG/AG genotypes have high seropositivity to thyroid peroxidase than thyroglobulin

IF 0.8 Q4 GENETICS & HEREDITY

Meta Gene Pub Date : 2022-02-01 DOI:10.1016/j.mgene.2022.101010

Nusrath Fathima , Qursheed Sultana , Syyeda Anees , Kaleem Ullah , Vitaly Ryu , Aleem Ahmed Khan , Mohammed Ishaq

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引用次数: 1

Abstract

Despite increasing burden of autoimmune thyroid disease (AITD) in India, there is paucity of data for the genetic susceptibility in Indian population which represents an important aspect to uncover the genetic factors for the cause or progression of different forms of AITD. It is known that production of auto-antibodies to thyroglobulin (TG) and thyroid peroxidase (TPO) is a characteristic feature of AITD. Production of high titres of auto-antibodies against TG and TPO is one the hallmarks of AITD that often precedes the development of clinical disease. The gene coding for cytotoxic T lymphocyte antigen-4 (CTLA-4) is considered an important candidate gene for susceptibility to AITD and also determines seropositivity. Earlier studies have demonstrated that polymorphic variants in CTLA-4 gene are linked to determine seropositivity. Hence, this study investigates a relationship between CTLA-4 (+49A/G) genotypes and seropositivity to anti-TPO and anti-TG antibodies in AITD patients. A total of 85 Indian participants were included and genotyped for CTLA-4 (+49A/G) single nucleotide polymorphism and further analyzed for seropositivity of TPO and TG auto-antibodies in mean age group of 35.76 ± 12.42 years of both the genders. The TPO seropositivity was reported higher in G allele containing genotypes GG and AG (66.66% and 58%, respectively) compared to AA (44.8%) genotype. Whereas, TG auto-antibodies showed highest seropositivity for genotype AA (55.17%) compared to AG (36%) and GG (16.6%). Higher median titre values were observed for TPO antibodies with GG genotype (1587.461 U/mL). In contrast to this, higher titres of auto-antibodies to TG were observed for individuals with AA genotype (520.746 U/mL). In conclusion, this study demonstrates a relationship between CTLA4 (+49A/G) genotype and seropositivity to thyroid auto antigens TPO and TG in Indian patients with clinical manifestation of Hashimoto's thyroiditis and Graves' disease, two different forms of AITD.

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CTLA-4 (+49A/G) GG/AG基因型自身免疫性甲状腺患者血清甲状腺过氧化物酶阳性高于甲状腺球蛋白阳性

尽管印度自身免疫性甲状腺疾病(AITD)的负担不断增加，但印度人群的遗传易感性数据缺乏，这是揭示不同形式AITD病因或进展的遗传因素的重要方面。众所周知，对甲状腺球蛋白(TG)和甲状腺过氧化物酶(TPO)的自身抗体的产生是AITD的一个特征。抗TG和TPO的高滴度自身抗体的产生是AITD的标志之一，通常先于临床疾病的发展。细胞毒性T淋巴细胞抗原-4 (CTLA-4)的基因编码被认为是AITD易感性的重要候选基因，也决定血清阳性。早期的研究表明，CTLA-4基因的多态性变异与血清阳性有关。因此，本研究探讨了CTLA-4 (+49A/G)基因型与AITD患者抗tpo和抗tg抗体血清阳性的关系。共纳入85名印度参与者，对CTLA-4 (+49A/G)单核苷酸多态性进行基因分型，并进一步分析TPO和TG自身抗体的血清阳性，平均年龄为35.76±12.42岁，男女均有。含GG和AG基因型的G等位基因TPO阳性率分别为66.66%和58%，高于AA基因型的44.8%。而TG自身抗体在AA基因型的血清阳性率最高(55.17%)，而AG(36%)和GG(16.6%)的血清阳性率最高。GG基因型TPO抗体滴度中值较高(1587.461 U/mL)。与此相反，AA基因型个体的TG自身抗体滴度较高(520.746 U/mL)。综上所述，本研究表明CTLA4 (+49A/G)基因型与印度临床表现为桥本甲状腺炎和Graves病两种不同形式AITD患者甲状腺自身抗原TPO和TG血清阳性的关系。

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来源期刊

Meta Gene Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

1.10

自引率

0.00%

发文量

期刊介绍： Meta Gene publishes meta-analysis, polymorphism and population study papers that are relevant to both human and non-human species. Examples include but are not limited to: (Relevant to human specimens): 1Meta-Analysis Papers - statistical reviews of the published literature of human genetic variation (typically linked to medical conditionals and/or congenital diseases) 2Genome Wide Association Studies (GWAS) - examination of large patient cohorts to identify common genetic factors that influence health and disease 3Human Genetics Papers - original studies describing new data on genetic variation in smaller patient populations 4Genetic Case Reports - short communications describing novel and in formative genetic mutations or chromosomal aberrations (e.g., probands) in very small demographic groups (e.g., family or unique ethnic group). (Relevant to non-human specimens): 1Small Genome Papers - Analysis of genetic variation in organelle genomes (e.g., mitochondrial DNA) 2Microbiota Papers - Analysis of microbiological variation through analysis of DNA sequencing in different biological environments 3Ecological Diversity Papers - Geographical distribution of genetic diversity of zoological or botanical species.