Journal of Cancer and Biomedical Research最新文献

Influence of Moringa oleifera flaxseed oil and atorvastatin on hyperlipidemic male albino rats 辣木、亚麻籽油和阿托伐他汀对高脂血症雄性白化大鼠的影响

Journal of Cancer and Biomedical Research Pub Date : 2019-06-01 DOI: 10.21608/JCBR.2019.37772

Somia Z Abdelhalim, M. Hegazi, Mohamed M.H. El-Bagoury

{"title":"Influence of Moringa oleifera flaxseed oil and atorvastatin on hyperlipidemic male albino rats","authors":"Somia Z Abdelhalim, M. Hegazi, Mohamed M.H. El-Bagoury","doi":"10.21608/JCBR.2019.37772","DOIUrl":"https://doi.org/10.21608/JCBR.2019.37772","url":null,"abstract":"The main aim of this study was to investigate the beneficial effects of lipitor (LIP), a commercial brand name of atorvastatin drug, flaxseed oil (FSO) and Moringa oleifera oil (MO), extract (ME) or meal (Mm) alone or in combination on hyperlipidemia induced in adult male albino rats. Rats were fed for 2 weeks on basal diet as a negative control group or fed on high fat diet (HFD) and a weekly dose of triton X-100 to induce hyperlipidemia as positive controls. The latter group was treated with: LIP, LIP + FSO, LIP + ME, FSO, FSO + ME, ME, MO or Mm for 4 weeks. At the end of the experiment, the livers were quickly excised and processed for analyzing the antioxidative enzymes activities including total superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione reductase (GR) and malondialdehyde (MDA). Feeding on MO and ME) showed no effects on the body weight or on anti-oxidative enzyme activities. Feeding on FSO, however, induced only increase in the body weight. ME of dry leaves and roots showed a decrease in the body weight and improved antioxidative enzymes activities. These effects of FSO, ME and MO on the body weight of rats may be attributed to captivity and reduction of the energy expenditure, while their marked effects on the antioxidative enzyme activity may need longer time of treatments. These results indicate that Moringa oleifera can be used in packed food supplement products.","PeriodicalId":381217,"journal":{"name":"Journal of Cancer and Biomedical Research","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131548088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Assessing mother’s antibiotics information by comparing internet and healthcare professionals as sources of informatics 通过比较互联网和卫生保健专业人员作为信息源来评估母亲的抗生素信息

Journal of Cancer and Biomedical Research Pub Date : 2019-06-01 DOI: 10.21608/JCBR.2019.37773

H. Kutrani, Antisar A Atiyah, H. M. Adam, H. A. Elhashmi

{"title":"Assessing mother’s antibiotics information by comparing internet and healthcare professionals as sources of informatics","authors":"H. Kutrani, Antisar A Atiyah, H. M. Adam, H. A. Elhashmi","doi":"10.21608/JCBR.2019.37773","DOIUrl":"https://doi.org/10.21608/JCBR.2019.37773","url":null,"abstract":"Parents especially mothers view the internet as a source to obtain information about their children’s health care. This study was designed to assess mother’s antibiotics information by comparing the Internet and healthcare professionals as sources of informatics. A cross-sectional descriptive study was performed in the outpatient pediatrics clinics at the Children Hospital and Benghazi Medical Centre, from February 2018 to June 2018. The sample size was 308 mothers attending the outpatient pediatrics clinic during the period of data collection. Knowledge/beliefs, behaviors, adherence, and awareness of antibiotic resistance were determined through the analysis of data obtained from the questionnaires. Eighty-two percent of mothers had a poor level of knowledge and beliefs regarding antibiotic use; however, about half of the mothers had good behaviors toward the antibiotic and a good level of awareness of antibiotics resistance. Despite this poor knowledge, 61.7% of the mothers had an overall good Adherence to antibiotic use. Furthermore, half of the mothers obtained their information about the antibiotic from the internet. A good knowledge level and good awareness of antibiotics resistance levels were attained from the Internet source. Meanwhile a good behavior and good adherence regarding antibiotic usage were gained from a healthcare professional. In conclusion, mother’s good information showed a significant difference existed between the Internet and the healthcare professional sources. Therefore, this study recommends that healthcare professionals should educate mothers concerning online information. Moreover, there is a need to improve mother’s knowledge about antibiotics uses.","PeriodicalId":381217,"journal":{"name":"Journal of Cancer and Biomedical Research","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123778911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Protective effect of Spirulina platensis on cyclophophamide- induced toxicity in experimental mice 螺旋藻对环磷酰胺致小鼠毒性的保护作用

Journal of Cancer and Biomedical Research Pub Date : 2019-06-01 DOI: 10.21608/JCBR.2019.37771

Rana Medhat, M. El-sheekh, A. M. Abo-Shady, M. Salem

{"title":"Protective effect of Spirulina platensis on cyclophophamide- induced toxicity in experimental mice","authors":"Rana Medhat, M. El-sheekh, A. M. Abo-Shady, M. Salem","doi":"10.21608/JCBR.2019.37771","DOIUrl":"https://doi.org/10.21608/JCBR.2019.37771","url":null,"abstract":"Spirulina platensis (SP) is a blue-green alga used as a dietary supplement as it possesses antioxidant, anti-inflammatory and hepatoprotective effects with clinical importance in several disorders. The aim of this study was to evaluate the ameliorating effects of SP extract on toxicity induced mice by treatment with the anticancer drug cyclophosphamide (CTX). To this end, SP was cultured under optimal conditions of growth at pH 9 for 9 days using Zarrouk,s medium. The active ingredients including pigments, phycobiliproteins and total soluble proteins were estimated in SP suspensions which were then dried to a powder form. To assess antitoxicity, adult male albino mice were treated with interperitoneal (i.p.) injection of PBS, single dose of CTX (4mg/mouse), CTX (i.p.) followed by subcutaneous (s.c.) injection of G-CSF (5μg/mouse) daily for 5 consecutive days or CTX (i.p.) followed by oral administration of 0.5 gm/mouse SP extract for 7 consecutive days. After 7 days of initial treatment with CTX, mice were sacrificed; blood, spleen, bone marrow and liver were harvested to assess CBC, total count for spleen and bone marrow and liver for biochemical analysis. The results showed that SP showed the highest yield of dry weight and pigment content C-phycocyanin (CPC), allophycocyanin (APC), phycoerthrin (PE) and total phycobiliproteins (phycobilins) at the 9th day of growth at pH 9 in culture. Oral administration of SP induced amelioration of CTX induced leucopenia in blood, bone marrow and spleen comparable to those of G-CSF. It also ameliorated the dysfunction in the liver enzymes ALT and AST. Interestingly, however, SP showed higher antioxidant effects than those of G-CSF as reflected by the higher activities of the anti-oxidant MDA and GSH. Conclusion: SP showed potent antitoxic effect through antioxidant activity and thus could be a useful co-adjuvant agent against chemotherapeutic drugs toxicity including cyclophosphamide. Article history:","PeriodicalId":381217,"journal":{"name":"Journal of Cancer and Biomedical Research","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121478151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochemical Alteration and Immunohistochemical Characterization of Lymphoid Cells Involved in Type 2 Diabetes Mellitus 2型糖尿病相关淋巴样细胞的生化改变和免疫组织化学特征

Journal of Cancer and Biomedical Research Pub Date : 2019-04-15 DOI: 10.21608/JCBR.2019.37782

Nahla H. El-shaer

{"title":"Biochemical Alteration and Immunohistochemical Characterization of Lymphoid Cells Involved in Type 2 Diabetes Mellitus","authors":"Nahla H. El-shaer","doi":"10.21608/JCBR.2019.37782","DOIUrl":"https://doi.org/10.21608/JCBR.2019.37782","url":null,"abstract":"T cells are crucial for the inflammation development of metabolic disorder and insulin resistance and the cytokines of T cells are considered as an excellent evaluating tools for the immune system activity in many diseases. As cytokine profile associates with the development of type-2 diabetes mellitus (T2-DM) is well understood the link between this disease and the involved immune cells still unclear. Therefore, it was hypothesized that Th1 pathway is disrupted in the pathogenesis of type 2DM. As such, we aimed to evaluate IL-12, which induces Th1 pathway, and IL-2 which mediates Th1 function. Paraffin-embedded sections of peripheral blood lymphocytes with T2-DM patients were stained with antibodies against CD4 and CD8. Computerized image analysis was used to calculate areas of stained lymphocytes taken from diabetic and control patients. Clinical data and blood samples were collected from Eighty-one (37.6±13.9 years old) diabetic Egyptian patients and 103 (40.5±12.7 years old) of normal control volunteer to evaluate the level of serum interleukin IL-2 and IL-12 in their blood by using ELISA technique. Routine laboratory analysis, including fasting blood glucose, CRP, lipid profile, fasting insulin, and CBC for each patient were measured. Immunohistochmical results in 2DM showed an increase in the numbers of CD8+ cells with a decrease in the numbers of CD4+ cells displayed. Diabetic patients showed decreased and increased values of IL-2 and IL_12, respectively as compared to control values. Conclusion: This data indicate the importance of these two cytokines in the pathogenesis of type-2 Diabetes mellitus. Article history: Received: March 18, 2019 Revised: April 5, 2019 Accepted: April 11, 2019","PeriodicalId":381217,"journal":{"name":"Journal of Cancer and Biomedical Research","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133070428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro Apoptotic effects of the medicinal plants Achillea santolina and Raphanus sativus extracts on different cancer cell lines 药用植物阿喀琉叶和莴苣提取物对不同癌细胞的体外凋亡作用

Journal of Cancer and Biomedical Research Pub Date : 2019-03-05 DOI: 10.21608/jcbr.2019.37759

R. Mohasseb, M. Salem, R. Gaafar, Mohamed Abd-Elbaseer

{"title":"In vitro Apoptotic effects of the medicinal plants Achillea santolina and Raphanus sativus extracts on different cancer cell lines","authors":"R. Mohasseb, M. Salem, R. Gaafar, Mohamed Abd-Elbaseer","doi":"10.21608/jcbr.2019.37759","DOIUrl":"https://doi.org/10.21608/jcbr.2019.37759","url":null,"abstract":"The extracts of the medicinal plants Achillea santolina and Raphanus sativus have been reported to show anti-cancer effects in vitro. However, the cellular and molecular mechanism of these effects are not clear yet. To compare the apoptotic effects of these plant extracts on different cancer cell lines in vitro. The phenolic, flavonoids and antioxidant activity were determined in the crude extracts. Then, Caco2 (colon adenocarcinoma), HepG2 (hepatic carcinoma), MCF7 (breast cancer) and the normal WISH (amniotic cell line) were treated in vitro with different concentrations of crude extracts for 72 hours. The half maximal inhibitory concentration (IC50) were detected by MTT assay, while cell cycle and apoptosis were assessed by flow cytometry. The methanolic extract of R. sativus seeds (cultivar: Balady) showed higher phenolic content (791.98 mg/d.wt) and higher antioxidant activity (93%) than those of the ethanolic extract of A. santolina (340.23 mg/d.wt) and (72.72%), respectively. R. sativus methanolic extract showed lower flavonoids contents (1.025 mg/g d.wt) than A. santolina ethanolic extract (24.66 mg/g d.wt). Treatment of Caco2, HepG2, MCF7 and WISH cell lines with A. santolina extract showed IC50 of 17.67 µg/ml, 15.12 µg/ml, 42.19 µg/ml and 50.99 µg/ml, respectively. While treatment of the same cell line with R. sativus showed IC50 of 40.77 µg/ml, 27.42 µg/ml, 54.16 µg/ml and 86.37 µg/ml respectively. A. santolina and R. sativus extracts induced similar cell cycle arrest in Caco2 at G1 phase by 42.4%. This study indicates that A. santolina has a potent anticancer activity against the selected cancer cell lines.","PeriodicalId":381217,"journal":{"name":"Journal of Cancer and Biomedical Research","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132264320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The therapeutic efficacy for targeting the PI3K signaling pathway for fighting breast, prostate and multiple myeloma cancer cells 靶向PI3K信号通路对抗乳腺癌、前列腺癌和多发性骨髓瘤细胞的疗效

Journal of Cancer and Biomedical Research Pub Date : 2019-03-01 DOI: 10.21608/JCBR.2019.37768

Gamal Badr, B. M. Badr

引用次数: 1

Association between HCV infection and the incidence of Diabetes Mellitus in Egyptians 埃及人丙型肝炎病毒感染与糖尿病发病率之间的关系

Journal of Cancer and Biomedical Research Pub Date : 2019-03-01 DOI: 10.21608/JCBR.2019.37766

Y. El-Bolkiny, F. K. Harras, Merveet A Mansour, Abdelhalim A. Alkhazendar, Mohamed Raghaey

{"title":"Association between HCV infection and the incidence of Diabetes Mellitus in Egyptians","authors":"Y. El-Bolkiny, F. K. Harras, Merveet A Mansour, Abdelhalim A. Alkhazendar, Mohamed Raghaey","doi":"10.21608/JCBR.2019.37766","DOIUrl":"https://doi.org/10.21608/JCBR.2019.37766","url":null,"abstract":"The present work was planned to find a correlation between HCV infection and diabetes mellitus (DM) incidence in Egyptian patients by finding a possible relationship between HCV antibodies and both anti-insulin antibodies (AIAs) and pancreatic islet cell antibodies (PICAs). The screening results showed that the percentage of HCV patients out of 200 subjects were 35.5 and 14.5% in males and females, respectively. Also, the association between DM and HCV were 13.21 and 13.64% in men and women, respectively. The titers of antibodies in diabetic or non-diabetic patients ranged from 30 - 58 for AIAs and from 30 - 40 for PICAs, The percentage of patients with raised serum transaminases was significantly more in DM patients with positive HCV antibody (52.3%) than DM patients with negative HCV antibody (16.7%). Conclusion, it can be concluded that diabetic or non-diabetic patients with HCV might have a rise titer of the antibodies toward insulin or its secretory cells in pancreas or toward both. where are more susceptible to both HCV-infection and antibodies than women, indicating to here is a significant association between HCV infection and induction of diabetes in Egyptians.","PeriodicalId":381217,"journal":{"name":"Journal of Cancer and Biomedical Research","volume":"71 3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129655098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic changes in naïve and polyclonal activated T-cell from naive mice 幼稚小鼠naïve和多克隆活化t细胞的代谢变化

Journal of Cancer and Biomedical Research Pub Date : 2018-12-01 DOI: 10.21608/jcbr.2018.37764

Asmaa Eltaramsy, M. Salem, Tarek M. Mohamed, M. Hegazi

{"title":"Metabolic changes in naïve and polyclonal activated T-cell from naive mice","authors":"Asmaa Eltaramsy, M. Salem, Tarek M. Mohamed, M. Hegazi","doi":"10.21608/jcbr.2018.37764","DOIUrl":"https://doi.org/10.21608/jcbr.2018.37764","url":null,"abstract":"We have found previously that early activated T cells show higher activities and antitumor effects than late activated T cells upon their adoptive transfer. However, the exact mechanism is not clear. This study aimed to investigate the impact of polyclonal activation on the metabolic signature of T cells from naive mice. Splenocytes of Swiss albino mice were harvested and cultured at 2.5 × 106/ml in T25 flasks in presence of (Con-A) and IL2 for 24h or 72h Then, cells were harvested and processed to determine activities of hexokinase (HK), phosphofructokinase (PFK), lactate dehydrogenase (LDH) and glucose 6 phosphate dehydrogenase(G6PD). The obtained data showed that activity of enzymes in activated cellswas increased after 24h and 72h activation as compared to naive cells, when and supernatant their level at 72h activation was higher than 24h activation. Comparison of enzymes activity between cells and supernatant showed higher activity of HK, G6PD and LDH in cells, While PFK activity was higher in supernatant after 72h.these results indicate that Upon the early activation, naive T cells have higher glycolytic pathway than late activation. These results have a great implication in adoptive T cell therapy","PeriodicalId":381217,"journal":{"name":"Journal of Cancer and Biomedical Research","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116964571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Trans Disciplinary Biomedical Research 跨学科生物医学研究

Journal of Cancer and Biomedical Research Pub Date : 2018-06-01 DOI: 10.21608/jcbr.2018.37755

M. Salem

引用次数: 0

Analysis of Micro RNA 29, 122 and 155 Gene Expression in Immune Cells of Egyptian Patients with Chronic Hepatitis C Virus Infection 埃及慢性丙型肝炎病毒感染者免疫细胞中微RNA 29、122和155基因表达分析

Journal of Cancer and Biomedical Research Pub Date : 2018-06-01 DOI: 10.21608/jcbr.2019.34736

M. Salem, S. El-Naggar, Abdel-Aziz A Zidan, Hasan Elbatei, Ezar H Hamed, H. Hamada

{"title":"Analysis of Micro RNA 29, 122 and 155 Gene Expression in Immune Cells of Egyptian Patients with Chronic Hepatitis C Virus Infection","authors":"M. Salem, S. El-Naggar, Abdel-Aziz A Zidan, Hasan Elbatei, Ezar H Hamed, H. Hamada","doi":"10.21608/jcbr.2019.34736","DOIUrl":"https://doi.org/10.21608/jcbr.2019.34736","url":null,"abstract":"Infected patients with chronic hepatitis C virus (HCV) showed high numbers of myeloid-derived suppressor cells (MDSCs) which are known to suppress the immune response. Given that microRNAs regulate the expression of many genes, it is important to test whether MDSCs correlate with altered miRNA expression. Aim: The present study aimed to assess the expression of miR-29, 122 and 155 in chronic HCV patients under interferon (IFN) therapy and their correlation with the frequency of MDCS. A total of 28 subjects, including eighteen chronic HCV patients, and 10 healthy volunteers were enrolled in this study. MiR-29, 122 and 155 were evaluated using real-timepolymerase chain reaction (RT-PCR). Flow cytometric analysis was performed to evaluate the MDSCs which defined as Lin-HLA-DRCD33+ CD11b+. Results: miR-29 and 122 expressions in HCV patients showed significant decrease when compared to their control. However, there was an increase (but non-significance) in miR-155 in HCV patients than control subjects. Both relative and absolute numbers of MDSCs were increased in the peripheral blood in HCV patients as compared to control subjects. Moreover, nonsignificance change in IFNα/β receptors was observed in HCV patients as compared to control subjects. Conclusion: High numbers of MDSCs in chronic HCV patients correlate with low expression of miR-29 and122. Article history:","PeriodicalId":381217,"journal":{"name":"Journal of Cancer and Biomedical Research","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116685162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0