IJC Heart and Vasculature最新文献

{"title":"Accelerated biological aging and incident degenerative valvular heart disease: Findings from 408,783 UK Biobank participants","authors":"Chaoyang Lin ,&nbsp;Enhao Wei ,&nbsp;Qianyao Lai ,&nbsp;Hangpan Jiang ,&nbsp;Maosen Lin ,&nbsp;Feng Hu ,&nbsp;Lin Fan ,&nbsp;Enhui Yao","doi":"10.1016/j.ijcha.2025.101838","DOIUrl":"10.1016/j.ijcha.2025.101838","url":null,"abstract":"<div><h3>Background</h3><div>Although prior studies have linked frailty and accelerated biological aging to aortic stenosis, comprehensive evidence across the spectrum of degenerative valvular diseases (VHD) and related clinical events remains unclear in middle-aged adults.</div></div><div><h3>Methods</h3><div>We analyzed 408,783 UK Biobank participants free of baseline valvular disease. Biological age accelerations (BAA) measures were derived from clinical traits using Phenotypic Age (PhenoAge) algorithm and the Klemera-Doubal method Biological Age (KDM-BA). Outcomes included incident aortic stenosis (AS), aortic regurgitation (AR), mitral regurgitation (MR), and related interventions or mortality.</div></div><div><h3>Results</h3><div>Over a median follow-up of 13.9 years, 10,364 incident degenerative VHD events (2.5 %) were documented, comprising 4602 AS, 1678 AS-related events, 1639 AR, and 4903 MR cases. Elevated BAA was significantly associated with higher AS risk. For PhenoAge, adjusted AS incidence rates (per 10,000 person-years; 95 % confidence interval) across quartiles (Q1–Q4) were 3.73 (3.37–4.12), 4.44 (4.05–4.88), 5.11 (4.67–5.59), and 7.79 (7.18–8.46), yielding an adjusted hazard ratio (HR) of 2.15 (1.96–2.35) for Q4. Comparable trends were observed for KDM-BA, with an adjusted HR of 1.98 (1.83–2.15) for Q4 vs Q1. AS-related events followed a similar pattern, with HRs of 1.80 (1.55–2.09) for PhenoAge Q4 and 2.22 (1.94–2.54) for KDM-BA Q4. Significant associations were also found for AR, AR-related events, and MR, but not for MR-related events.</div></div><div><h3>Conclusions</h3><div>Among middle-aged adults, both BAA metrics were associated with increased risks of degenerative VHD and related adverse events, except for MR-related events. These findings highlight BAA as a potential tool for early risk stratification and targeted prevention.</div></div>","PeriodicalId":38026,"journal":{"name":"IJC Heart and Vasculature","volume":"61 ","pages":"Article 101838"},"PeriodicalIF":2.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145519471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sex differences in cardiac sarcoidosis","authors":"Katrina A. Williamson ,&nbsp;Jenna M. Davison ,&nbsp;Andrew N. Rosenbaum ,&nbsp;Panithaya Chareonthaitawee ,&nbsp;Nikhil Kolluri ,&nbsp;John P. Bois ,&nbsp;Omar F Abou Ezzeddine ,&nbsp;John A. Schirger ,&nbsp;Suraj Kapa ,&nbsp;Konstantinos C. Siontis ,&nbsp;Leslie T. Cooper ,&nbsp;Courtney A. Arment","doi":"10.1016/j.ijcha.2025.101777","DOIUrl":"10.1016/j.ijcha.2025.101777","url":null,"abstract":"<div><h3>Background</h3><div>Cardiac sarcoidosis (CS) is an inflammatory cardiomyopathy for which sex differences outcomes are not well described.</div></div><div><h3>Objectives</h3><div>This study aimed to understand differences in CS presentation, complications, and outcomes between females and males.</div></div><div><h3>Methods</h3><div>Patients meeting Japanese Circulation Society or Heart Rhythm Society criteria for CS were evaluated at a single institution from January 1, 1999, to December 31, 2023 (n = 455). Presenting characteristics including demographics, symptoms, conduction abnormalities, arrhythmias and left ventricular ejection fraction (LVEF) between females and males were compared. Sex-based differences in hospitalizations, LVAD implantation, transplantation and death were analyzed.</div></div><div><h3>Results</h3><div>The cohort was mostly male (60.7 %). Females presented at an older age (58.5, IQR 51–65 in females, vs 54, IQR 45–61 in males, <em>p</em> = 0.001), and more females experienced palpitations (46.4 %, <em>p</em> = 0.04), chest pain (34.6 % <em>p</em> = 0.02), and fatigue (53.6 % <em>p</em> = 0.01) at presentation. Females had higher New York Heart Association (NYHA) class (III or IV) (31.5 % vs 21.8 %; <em>p</em> = 0.05), and higher NT-proBNP (467 in females vs 257.5 in males, <em>p</em> = 0.03) at presentation. Cardiovascular hospitalization–free survival, LVAD/transplant-free survival, and overall survival were not significantly different.</div></div><div><h3>Conclusions</h3><div>Females presented at an older age and were more symptomatic with higher NYHA class symptoms than males, suggesting that females are presenting or being diagnosed with CS later than males. LVEF, rates of heart block, and ventricular arrhythmias were similar between sexes, suggesting greater heart failure severity in females with CS may be related to greater left ventricular stiffening or worse valvular disease. Differences in survival outcomes were not significant.</div></div>","PeriodicalId":38026,"journal":{"name":"IJC Heart and Vasculature","volume":"61 ","pages":"Article 101777"},"PeriodicalIF":2.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144917252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Linking factor XIII activity to all-cause mortality after myocardial infarction: the overlooked role of serum albumin","authors":"Jan Traub ,&nbsp;Makram Abu Hussein ,&nbsp;Dominik Schmitt ,&nbsp;Anna Frey","doi":"10.1016/j.ijcha.2025.101796","DOIUrl":"10.1016/j.ijcha.2025.101796","url":null,"abstract":"<div><h3>Background</h3><div>Acute myocardial infarction (MI) remains a major cause of morbidity and mortality despite therapeutic advances. Factor XIII (FXIII), a fibrin-stabilizing enzyme with roles in coagulation, inflammation, and tissue repair, has emerged as a potential biomarker in MI. While low FXIII activity has been linked to adverse outcomes, the underlying determinants and its independent prognostic value remain unclear.</div></div><div><h3>Methods</h3><div>In this retrospective study, FXIII activity was measured in 926 MI patients treated at University Hospital Würzburg between 2018 and 2023. Blood samples were collected within 24 h of cardiac catheterization. FXIII activity was assessed photometrically, and patients were followed for all-cause mortality. Multivariable regression and Cox models were used to identify predictors of FXIII activity and mortality.</div></div><div><h3>Results</h3><div>Median FXIII activity was 110 %. Lower FXIII activity was associated with older age, female sex, lower albumin, higher CRP, and reduced kidney function. While crude mortality at 30 days and 1 year was significantly higher in patients with FXIII ≤ 110 %, FXIII activity was not an independent predictor of mortality after adjustment. Key predictors included albumin (HR = 0.221, p &lt; 0.001), age (HR = 1.048, p &lt; 0.001), eGFR (HR = 0.988, p = 0.001), and ASAT (HR = 1.001, p = 0.002).</div></div><div><h3>Conclusions</h3><div>Although lower FXIII activity is associated with higher mortality post-MI, this effect is largely mediated by albumin levels. Albumin appears to be a central determinant of both FXIII activity and prognosis, highlighting its potential role as a key marker in risk stratification. Further studies are warranted to explore therapeutic implications of hypoalbuminemia in MI.</div></div>","PeriodicalId":38026,"journal":{"name":"IJC Heart and Vasculature","volume":"61 ","pages":"Article 101796"},"PeriodicalIF":2.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145050040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of comorbidity awareness in patients with atrial fibrillation: The ACAPAF study","authors":"Rana Önder ,&nbsp;Lien Desteghe ,&nbsp;Johan Vijgen ,&nbsp;Hein Heidbuchel","doi":"10.1016/j.ijcha.2025.101813","DOIUrl":"10.1016/j.ijcha.2025.101813","url":null,"abstract":"<div><h3>Background and aim</h3><div>Systematic and integrated comorbidity management in patients with atrial fibrillation (AF) requires patient involvement, starting with comorbidity awareness. This study evaluates comorbidity awareness in patients with AF before and after a first nurse-led AF clinic visit and after six months. We also measured the time needed for full comorbidity mapping using the EHRA-PATHS software.</div></div><div><h3>Methods</h3><div>This prospective two-centre study included patients diagnosed with AF attending the AF clinic for the first time. The software systematically assessed 23 comorbidities. Patients completed a comorbidity awareness questionnaire, focusing on nine AF-related comorbidities two weeks before their first visit, less than a week after, and six months later. Patients also had a telephone consultation with the AF nurse to discuss their comorbidities 1–3 months post-visit.</div></div><div><h3>Results</h3><div>The study included 76 patients (mean age 68.3 ± 10.3 y). Baseline awareness of comorbidity relevance for AF ranged between 11.1–100.0 %. Awareness about own alcohol consumption was the most ‘underestimated’ comorbidity before first contact (50.0 %), while smoking was most ‘overestimated’ (55.6 %; i.e. admitted in private but not during formal evaluation by nurses). The impact of an AF clinic visit on awareness of personal comorbidities was limited (p = 0.456), and also after an additional phone consultation, awareness was suboptimal after six months (p = 0.099). AF nurses needed 18.4 ± 8.7 min to complete the software.</div></div><div><h3>Conclusions</h3><div>Patients’ comorbidity awareness is moderate, and more educational efforts are needed to improve their awareness. A systematic and complete comorbidity evaluation at the AF clinic using EHRA-PATHS software can be done within a reasonable time frame.</div></div>","PeriodicalId":38026,"journal":{"name":"IJC Heart and Vasculature","volume":"61 ","pages":"Article 101813"},"PeriodicalIF":2.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145220614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of miR-21-5p in the pathogenesis of abdominal aortic aneurysm regarding the Th17 pathway","authors":"Dorota Studzińska ,&nbsp;Sabina Lichołai ,&nbsp;Kamil Polok ,&nbsp;Hanna Plutecka ,&nbsp;Piotr Kica ,&nbsp;Piotr Grazda ,&nbsp;Maciej Chwała ,&nbsp;Marek Sanak ,&nbsp;Wojciech Szczeklik","doi":"10.1016/j.ijcha.2025.101821","DOIUrl":"10.1016/j.ijcha.2025.101821","url":null,"abstract":"<div><h3>Background and aims</h3><div>The interplay between Th17-related immune activity and miRNA-driven regulation in abdominal aortic aneurysm (AAA) pathogenesis remains poorly understood. In this prospective study, we aimed to assess the potential role of miR-21-5p corresponding to the Th17 pathway disturbances in the development of AAA.</div></div><div><h3>Methods</h3><div>Biological samples were collected from patients with true infrarenal AAA, undergoing elective open abdominal aortic surgery, and patients with peripheral arterial disease (PAD) without concomitant aneurysms on the day of hospital admission for surgery. A fragment of aneurysmal abdominal aortic tissue was collected from patients intraoperatively. Total RNA was extracted from the samples, and the expression level of miR-21-5p and selected markers associated with Th17 pathway were assessed using quantitative real-time PCR (RT-qPCR) and Luminex assays, respectively. Moreover, we performed an in vitro model using human endothelial cells, transfected with a synthetic miR-21-5p mimic.</div></div><div><h3>Results</h3><div>We included 60 patients (30 in the study group and 30 in the control group, selected based on the propensity score matching method). Circulating miR-21-5p levels were significantly higher in AAA patients compared to controls. miR-21-5p downregulates genes involved in endothelial development, regulation of vascular permeability, or endothelial cell response to growth factors. We demonstrated that the Th17 circulating mediators were higher among AAA patients compared to controls, and were higher in the middle part of the aneurysmal aorta when compared to its upper pole.</div></div><div><h3>Conclusion</h3><div>Our study showed that, regarding AAA pathogenesis, endothelial dysfunction followed by inflammation could be mediated by an increased level of miR-21-5p.</div></div>","PeriodicalId":38026,"journal":{"name":"IJC Heart and Vasculature","volume":"61 ","pages":"Article 101821"},"PeriodicalIF":2.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145267282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cardamonin attenuates angiotensin II-induced abdominal aortic aneurysms through activation of the Nrf2/HO-1 pathway","authors":"Hsiao-Ya Tsai ,&nbsp;Yu-Juei Hsu ,&nbsp;Chih-Yuan Lin ,&nbsp;Po-Hsun Huang ,&nbsp;Chin-Wang Hsu ,&nbsp;Shih-Hung Tsai","doi":"10.1016/j.ijcha.2025.101815","DOIUrl":"10.1016/j.ijcha.2025.101815","url":null,"abstract":"<div><h3>Background</h3><div>Cardamonin is a natural chalcone compound. It has been shown to have various pharmacological properties. Cardamonin can activate the Nrf2 signaling pathway. Abdominal aortic aneurysm (AAA) is a complex degenerative aortic disease. Ruptured AAAs continue to be among the leading causes of sudden death in elderly individuals. No medical therapy has proven clinical benefits for preventing AAA progression. Therefore, an adjunctive medical therapy is essential to address this unmet clinical need.</div></div><div><h3>Methods</h3><div>Human aortic smooth muscle cells (HASMCs) were used to determine the molecular mechanism of cardamonin. A murine model of angiotensin II (AngII)-induced AAA was used to assess the therapeutic effects of cardamonin on AAA growth in apolipoprotein E knockout (ApoE KO) mice <em>in vivo</em>. Immunoblotting, senescence assays, and reactive oxygen species (ROS) production assays were used to determine the protective effects of cardamonin <em>in vivo</em> and <em>in vitro</em>.</div></div><div><h3>Results</h3><div>Cardamonin induced Nrf2 translocation from the cytosol to the nuclear compartment in HASMCs. Cardamonin reduced AngII-induced ROS production and matrix metalloproteinases (MMPs) overexpression through activation of the Nrf2/heme oxygenase-1 (HO-1) antioxidant pathway in HASMCs. Silencing HO-1 attenuated the anti-ROS effects of cardamonin and abolished the protective effects of cardamonin in AngII-challenged HASMCs. Cardamonin (20 mg/kg/day) reduced AngII-induced AAA <em>in vivo</em>. Cardamonin also reduced the overexpression of MMPs and the production of ROS and attenuated elastin degradation in aortic tissues.</div></div><div><h3>Conclusion</h3><div>Cardamonin inhibits the progression of AngII-induced AAA through Nrf2/HO-1-mediated antioxidant and anti-inflammatory pathways. Cardamonin could have the potential to be an adjunctive therapy for small AAAs.</div></div>","PeriodicalId":38026,"journal":{"name":"IJC Heart and Vasculature","volume":"61 ","pages":"Article 101815"},"PeriodicalIF":2.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145267287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution of coronary artery calcium in a large European all-comer population referred for cardiac imaging","authors":"Julia Duelli,&nbsp;Christoph Ryffel,&nbsp;Magdalena Stuetz,&nbsp;Raffael Ghenzi,&nbsp;Marko Gajic,&nbsp;Dimitrios Moysidis,&nbsp;Dominik C. Benz,&nbsp;Aju P. Pazhenkottil,&nbsp;Andreas A. Giannopoulos,&nbsp;Philipp A. Kaufmann,&nbsp;Ronny R. Buechel","doi":"10.1016/j.ijcha.2025.101792","DOIUrl":"10.1016/j.ijcha.2025.101792","url":null,"abstract":"<div><h3>Background and aims</h3><div>The coronary artery calcium score (CACS) is a well-established surrogate marker of atherosclerotic plaque burden and is highly valuable for risk stratification. However, contemporary data on the distribution of CACS across age and sex is lacking, particularly for European countries and quantified by multi-slice CT. We assessed predictors of CACS and provide granular age- and sex-specific reference values derived from a real-world clinical population referred for cardiac imaging.</div></div><div><h3>Methods</h3><div>This single-center, retrospective study examined patients clinically referred for non-invasive cardiac imaging from May 2013 to May 2024. Patients without coronary artery disease, cardiomyopathy, cardiac surgery or intervention, renal or hepatic failure were included. Multiple linear regression was used to identify independent predictors of CACS, and predictor importance was calculated. CACS percentiles were then calculated for both sexes and stratified across nine age groups.</div></div><div><h3>Results</h3><div>The final population consisted of 18′225 individuals (39.6 % women; 60.8 % symptomatic). Age and sex were the most influential predictors of CACS, accounting for 69 % and 18 % relative predictor importance, respectively. Men exhibited a significantly higher median CACS than women (59 [IQR 1–338] vs. 10 [IQR 0–129], <em>p</em> &lt; 0.001) across all age groups.</div></div><div><h3>Conclusions</h3><div>This study provides contemporary age- and sex-based CAC score reference values, as observed in a large European real-world cohort referred for cardiac imaging. Age and sex exhibit the most relevant impact on expected CAC scores, while other conventional risk factors appear to be less important. Our results enable improved classification regarding the coronary calcium burden.</div></div>","PeriodicalId":38026,"journal":{"name":"IJC Heart and Vasculature","volume":"61 ","pages":"Article 101792"},"PeriodicalIF":2.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145007675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent highlights from the International Journal of Cardiology: Heart & Vasculature: Valvular heart disease","authors":"Francesco Pelliccia,&nbsp;Artur Dziewierz,&nbsp;Marco Zimarino,&nbsp;Dobromir Dobrev","doi":"10.1016/j.ijcha.2025.101828","DOIUrl":"10.1016/j.ijcha.2025.101828","url":null,"abstract":"","PeriodicalId":38026,"journal":{"name":"IJC Heart and Vasculature","volume":"61 ","pages":"Article 101828"},"PeriodicalIF":2.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145362287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical utility of segmental native T1 mapping for diagnosis and risk-stratification in cardiac sarcoidosis","authors":"Hidehiro Iwakawa ,&nbsp;Nobuhiro Suzuki ,&nbsp;Hirokazu Yoshida ,&nbsp;Yohei Sasaki ,&nbsp;Ryosuke Kato ,&nbsp;Ryota Kaimori ,&nbsp;Hiroyuki Watanabe","doi":"10.1016/j.ijcha.2025.101787","DOIUrl":"10.1016/j.ijcha.2025.101787","url":null,"abstract":"<div><h3>Background</h3><div>The clinical utility and optimal analytical approach for native T1 mapping in cardiac sarcoidosis (CS) remain unclear. This study investigated the clinical value of segmental native T1 assessment in patients with CS.</div></div><div><h3>Methods</h3><div>We recruited 55 participants, including 41 patients undergoing diagnostic evaluation of CS and 14 healthy controls. Of the 41 patients, 29 were diagnosed with CS and 12 were classified as non-CS. Segmental cardiac magnetic resonance findings of the left ventricle were evaluated using a 1.5-T scanner. The primary endpoint was a composite of all-cause death, fatal ventricular arrhythmia, bradycardia, or hospitalization for cardiovascular events.</div></div><div><h3>Results</h3><div>Maximum and global native T1 values were significantly higher in CS patients than in healthy controls and non-CS patients. Maximum and global T1 values demonstrated comparable predictive performance in differentiating CS from the other groups, with areas under the curve (AUCs) of 0.92 and 0.90, respectively. The AUC for predicting segments with late gadolinium enhancement (LGE) was highest for extracellular volume, followed by native T1 and T2 (<em>P</em> &lt; 0.05 for all). Patients with six or more segments showing native T1 ≥ 1091 ms had a significantly worse prognosis than those without (55.6 % vs. 18.2 %, <em>P</em> = 0.028).</div></div><div><h3>Conclusions</h3><div>Maximum and global native T1 values were highly predictive for differentiating CS. The high segmental native T1 values in patients with CS may reflect the regional presence of LGE. Segmental native T1 assessment can aid in estimating long-term outcomes.</div></div>","PeriodicalId":38026,"journal":{"name":"IJC Heart and Vasculature","volume":"61 ","pages":"Article 101787"},"PeriodicalIF":2.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144917251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated proteomics and metabolomics analyses reveal potential molecular signatures of rabbit atherosclerotic plaques","authors":"Chunfang Zan ,&nbsp;Tianxiong Ji ,&nbsp;Xinyu Zhang ,&nbsp;Min-Fu Yang ,&nbsp;Zhifang Wu ,&nbsp;Sijin Li","doi":"10.1016/j.ijcha.2025.101840","DOIUrl":"10.1016/j.ijcha.2025.101840","url":null,"abstract":"<div><h3>Objective</h3><div>This study aims to explore potential mechanisms associated with differentially abundant proteins and metabolites in rabbit plaques through integrated proteomics and untargeted metabolomics analyses.</div></div><div><h3>Methods</h3><div>Experimental rabbits were randomly divided into the model group and the sham group. Abdominal aortas were isolated, collected, and treated with proteinase K. Subsequently, a tandem mass tag (TMT)-labeled quantitative proteomics analysis and an untargeted metabolomics analysis via liquid chromatography-mass spectrometry (LC-MS) of abdominal aortas were performed to evaluate the possible protein and metabolite fingerprints in arterial plaques. Acquired data were analyzed using uni‐ and multivariate statistics. The correlation between differentially abundant proteins and metabolites was analyzed using the <em>Pearson</em> correlation coefficient strategy, and their possibly involved functional pathways were predicted by Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis.</div></div><div><h3>Results</h3><div>Advanced plaques develop in the model group. A total of 207 proteins are significantly altered in injured aortas compared to uninjured ones, with 133 upregulated and 74 downregulated proteins (fold changes &gt; 1.2, <em>P</em> &lt; 0.05). In plaques, 234 metabolites are significantly changed under the positive ion mode, and 187 under the negative ion mode. Notably, increases are observed in phosphatidylcholines (PCs) [PC 9:0] and lysophosphatidylcholines (LPCs) [LPC 20:2], two key lipid components. These metabolites are involved in some key metabolic pathways, including purine metabolism and vascular smooth muscle contraction.</div></div><div><h3>Conclusions</h3><div>The results confirm the potential of integrated proteomics and untargeted metabolomics in exploring the molecular characteristics of atherosclerosis. Identified proteins and metabolites may serve as promising biomarkers for plaque diagnosis.</div></div>","PeriodicalId":38026,"journal":{"name":"IJC Heart and Vasculature","volume":"61 ","pages":"Article 101840"},"PeriodicalIF":2.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145519603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}